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UniProtKB - P14377 (ZRAS_ECOLI)
Protein
Sensor protein ZraS
Gene
zraS
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system ZraS/ZraR. May function as a membrane-associated protein kinase that phosphorylates ZraR in response to high concentrations of zinc or lead in the medium.
1 PublicationCaution
Was originally thought to be involved in the regulation of the labile hydrogenase activity.1 Publication
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphorelay sensor kinase activity Source: InterPro
- protein histidine kinase activity Source: EcoCyc
GO - Biological processi
- cellular response to cell envelope stress Source: EcoCyc
- cellular response to lead ion Source: EcoCyc
- cellular response to zinc ion Source: EcoCyc
- peptidyl-histidine phosphorylation Source: EcoCyc
- protein autophosphorylation Source: EcoCyc
- signal transduction Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:HYDH-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:zraS Synonyms:hydH Ordered Locus Names:b4003, JW3967 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 15 – 35 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 202 – 222 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074909 | 1 – 465 | Sensor protein ZraSAdd BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 254 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Autophosphorylated.
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P14377 |
PRIDEi | P14377 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263453, 21 interactors |
DIPi | DIP-9981N |
IntActi | P14377, 1 interactor |
STRINGi | 511145.b4003 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 251 – 458 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 208 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG4191, Bacteria |
HOGENOMi | CLU_000445_89_29_6 |
InParanoidi | P14377 |
OMAi | QAIFTPY |
PhylomeDBi | P14377 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P14377-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFMQRSKDS LAKWLSAILP VVIVGLVGLF AVTVIRDYGR ASEADRQALL
60 70 80 90 100
EKGNVLIRAL ESGSRVGMGM RMHHVQQQAL LEEMAGQPGV LWFAVTDAQG
110 120 130 140 150
IIILHSDPDK VGRALYSPDE MQKLKPEENS RWRLLGKTET TPALEVYRLF
160 170 180 190 200
QPMSAPWRHG MHNMPRCNGK AVPQVDAQQA IFIAVDASDL VATQSGEKRN
210 220 230 240 250
TLIILFALAT VLLASVLSFF WYRRYLRSRQ LLQDEMKRKE KLVALGHLAA
260 270 280 290 300
GVAHEIRNPL SSIKGLAKYF AERAPAGGEA HQLAQVMAKE ADRLNRVVSE
310 320 330 340 350
LLELVKPTHL ALQAVDLNTL INHSLQLVSQ DANSREIQLR FTANDTLPEI
360 370 380 390 400
QADPDRLTQV LLNLYLNAIQ AIGQHGVISV TASESGAGVK ISVTDSGKGI
410 420 430 440 450
AADQLDAIFT PYFTTKAEGT GLGLAVVHNI VEQHGGTIQV ASQEGKGSTF
460
TLWLPVNITR KDPQG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 361 | L → V in AAA24003 (PubMed:2666400).Curated | 1 | |
Sequence conflicti | 383 – 387 | SESGA → TKAG in AAA24003 (PubMed:2666400).Curated | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43101.1 U00096 Genomic DNA Translation: AAC76977.1 AP009048 Genomic DNA Translation: BAE77316.1 M28369 Genomic DNA Translation: AAA24003.1 |
PIRi | F65207 |
RefSeqi | NP_418431.1, NC_000913.3 WP_001211892.1, NZ_SSZK01000047.1 |
Genome annotation databases
EnsemblBacteriai | AAC76977; AAC76977; b4003 BAE77316; BAE77316; BAE77316 |
GeneIDi | 948506 |
KEGGi | ecj:JW3967 eco:b4003 |
PATRICi | fig|1411691.4.peg.2707 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43101.1 U00096 Genomic DNA Translation: AAC76977.1 AP009048 Genomic DNA Translation: BAE77316.1 M28369 Genomic DNA Translation: AAA24003.1 |
PIRi | F65207 |
RefSeqi | NP_418431.1, NC_000913.3 WP_001211892.1, NZ_SSZK01000047.1 |
3D structure databases
AlphaFoldDBi | P14377 |
SMRi | P14377 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263453, 21 interactors |
DIPi | DIP-9981N |
IntActi | P14377, 1 interactor |
STRINGi | 511145.b4003 |
Proteomic databases
PaxDbi | P14377 |
PRIDEi | P14377 |
Genome annotation databases
EnsemblBacteriai | AAC76977; AAC76977; b4003 BAE77316; BAE77316; BAE77316 |
GeneIDi | 948506 |
KEGGi | ecj:JW3967 eco:b4003 |
PATRICi | fig|1411691.4.peg.2707 |
Organism-specific databases
EchoBASEi | EB0008 |
Phylogenomic databases
eggNOGi | COG4191, Bacteria |
HOGENOMi | CLU_000445_89_29_6 |
InParanoidi | P14377 |
OMAi | QAIFTPY |
PhylomeDBi | P14377 |
Enzyme and pathway databases
BioCyci | EcoCyc:HYDH-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Miscellaneous databases
PROi | PR:P14377 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZRAS_ECOLI | |
Accessioni | P14377Primary (citable) accession number: P14377 Secondary accession number(s): Q2M8U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |