UniProtKB - P14373 (TRI27_HUMAN)
Zinc finger protein RFP
TRIM27
Functioni
E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.
4 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 96 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 99 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 118 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 124 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 57 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 96 – 127 | B box-typePROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- identical protein binding Source: UniProtKB
- metal ion binding Source: ProtInc
- nucleic acid binding Source: ProtInc
- protein homodimerization activity Source: GO_Central
- protein kinase binding Source: GO_Central
- SUMO transferase activity Source: Reactome
- transcription coactivator activity Source: ARUK-UCL
- ubiquitin protein ligase activity Source: Reactome
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- Arp2/3 complex-mediated actin nucleation Source: UniProtKB
- innate immune response Source: UniProtKB
- negative regulation of adaptive immune response Source: UniProtKB
- negative regulation of calcium ion import Source: UniProtKB
- negative regulation of gene expression, epigenetic Source: MGI
- negative regulation of interleukin-2 production Source: UniProtKB
- negative regulation of protein kinase activity Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- negative regulation of tumor necrosis factor production Source: UniProtKB
- negative regulation of viral transcription Source: UniProtKB
- positive regulation of actin nucleation Source: Ensembl
- positive regulation of autophagy Source: GO_Central
- positive regulation of DNA-binding transcription factor activity Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: GO_Central
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: GO_Central
- protein K63-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
- protein ubiquitination Source: GO_Central
- regulation of gene expression Source: GO_Central
- regulation of protein localization Source: GO_Central
- regulation of viral entry into host cell Source: GO_Central
- retrograde transport, endosome to Golgi Source: UniProtKB
- spermatogenesis Source: ProtInc
- suppression of viral release by host Source: Ensembl
Keywordsi
Molecular function | DNA-binding, Repressor, Transferase |
Biological process | Transcription, Transcription regulation, Transport, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P14373 |
Reactomei | R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-8948751, Regulation of PTEN stability and activity R-HSA-9635465, Suppression of apoptosis |
SignaLinki | P14373 |
SIGNORi | P14373 |
Protein family/group databases
MoonDBi | P14373, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein RFP (EC:2.3.2.27)Alternative name(s): RING finger protein 76 RING-type E3 ubiquitin transferase TRIM27Curated Ret finger protein Tripartite motif-containing protein 27 |
Gene namesi | Name:TRIM27 Synonyms:RFP, RNF76 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9975, TRIM27 |
MIMi | 602165, gene |
neXtProti | NX_P14373 |
VEuPathDBi | HostDB:ENSG00000204713 |
Subcellular locationi
Nucleus
Endosome
- Early endosome 3 Publications
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853).By similarity1 Publication
Cytosol
- cytosol Source: GO_Central
Endosome
- early endosome Source: UniProtKB-SubCell
- endosome Source: UniProtKB
Nucleus
- fibrillar center Source: HPA
- nuclear membrane Source: MGI
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- PML body Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endosome, NucleusPathology & Biotechi
Involvement in diseasei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 315 – 316 | Breakpoint for translocation to form the TRIM27/RET oncogene | 2 |
Keywords - Diseasei
Proto-oncogeneOrganism-specific databases
DisGeNETi | 5987 |
MalaCardsi | TRIM27 |
OpenTargetsi | ENSG00000204713 |
Orphaneti | 146, Differentiated thyroid carcinoma |
PharmGKBi | PA162406956 |
Miscellaneous databases
Pharosi | P14373, Tbio |
Genetic variation databases
BioMutai | TRIM27 |
DMDMi | 132517 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056240 | 1 – 513 | Zinc finger protein RFPAdd BLAST | 513 |
Proteomic databases
EPDi | P14373 |
jPOSTi | P14373 |
MassIVEi | P14373 |
MaxQBi | P14373 |
PaxDbi | P14373 |
PeptideAtlasi | P14373 |
PRIDEi | P14373 |
ProteomicsDBi | 53047 [P14373-1] 53048 [P14373-2] |
PTM databases
GlyGeni | P14373, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P14373 |
MetOSitei | P14373 |
PhosphoSitePlusi | P14373 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000204713, Expressed in right lobe of liver and 235 other tissues |
ExpressionAtlasi | P14373, baseline and differential |
Genevisiblei | P14373, HS |
Organism-specific databases
HPAi | ENSG00000204713, Low tissue specificity |
Interactioni
Subunit structurei
Homomultimerizes.
Interacts with PML, EIF3S6, EPC1, CHD4 and EID1.
Interacts with MAGED4, MAGEF1 and MAGEL2.
Interacts with herpes simplex virus protein ICP0.
10 Publications(Microbial infection) Interacts with M.tuberculosis PtpA, whick blocks TRIM27-promoted JNK/p38 MAPK pathway activation and cell apoptosis.
1 PublicationBinary interactionsi
P14373
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: GO_Central
- protein kinase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111919, 508 interactors |
CORUMi | P14373 |
IntActi | P14373, 418 interactors |
MINTi | P14373 |
STRINGi | 9606.ENSP00000366404 |
Miscellaneous databases
RNActi | P14373, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 298 – 492 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 195 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 132 – 172 | Sequence analysisAdd BLAST | 41 | |
Coiled coili | 282 – 311 | Sequence analysisAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 57 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 96 – 127 | B box-typePROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000158537 |
HOGENOMi | CLU_013137_6_1_1 |
InParanoidi | P14373 |
OMAi | ICCACLT |
PhylomeDBi | P14373 |
TreeFami | TF350411 |
Family and domain databases
CDDi | cd15814, SPRY_PRY_TRIM27, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035791, SPRY/PRY_TRIM27 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR020457, Znf_B-box_chordata IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit |
PRINTSi | PR01406, BBOXZNFINGER PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASGSVAECL QQETTCPVCL QYFAEPMMLD CGHNICCACL ARCWGTAETN
60 70 80 90 100
VSCPQCRETF PQRHMRPNRH LANVTQLVKQ LRTERPSGPG GEMGVCEKHR
110 120 130 140 150
EPLKLYCEED QMPICVVCDR SREHRGHSVL PLEEAVEGFK EQIQNQLDHL
160 170 180 190 200
KRVKDLKKRR RAQGEQARAE LLSLTQMERE KIVWEFEQLY HSLKEHEYRL
210 220 230 240 250
LARLEELDLA IYNSINGAIT QFSCNISHLS SLIAQLEEKQ QQPTRELLQD
260 270 280 290 300
IGDTLSRAER IRIPEPWITP PDLQEKIHIF AQKCLFLTES LKQFTEKMQS
310 320 330 340 350
DMEKIQELRE AQLYSVDVTL DPDTAYPSLI LSDNLRQVRY SYLQQDLPDN
360 370 380 390 400
PERFNLFPCV LGSPCFIAGR HYWEVEVGDK AKWTIGVCED SVCRKGGVTS
410 420 430 440 450
APQNGFWAVS LWYGKEYWAL TSPMTALPLR TPLQRVGIFL DYDAGEVSFY
460 470 480 490 500
NVTERCHTFT FSHATFCGPV RPYFSLSYSG GKSAAPLIIC PMSGIDGFSG
510
HVGNHGHSME TSP
The sequence of this isoform differs from the canonical sequence as follows:
354-358: FNLFP → SPSTT
359-513: Missing.
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JIZ4 | A0A0G2JIZ4_HUMAN | RING-type E3 ubiquitin transferase | TRIM27 | 249 | Annotation score: | ||
H0Y551 | H0Y551_HUMAN | Zinc finger protein RFP | TRIM27 | 248 | Annotation score: | ||
A0A140T9V9 | A0A140T9V9_HUMAN | Zinc finger protein RFP | TRIM27 | 247 | Annotation score: | ||
A0A140T9B9 | A0A140T9B9_HUMAN | Zinc finger protein RFP | TRIM27 | 207 | Annotation score: | ||
A0A140T9N6 | A0A140T9N6_HUMAN | Zinc finger protein RFP | TRIM27 | 231 | Annotation score: | ||
A0A140T9Q6 | A0A140T9Q6_HUMAN | Zinc finger protein RFP | TRIM27 | 52 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 446 | E → K in AAH66924 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010896 | 354 – 358 | FNLFP → SPSTT in isoform Beta. 1 Publication | 5 | |
Alternative sequenceiVSP_010897 | 359 – 513 | Missing in isoform Beta. 1 PublicationAdd BLAST | 155 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03407 mRNA Translation: AAA36564.1 AF230393 mRNA Translation: AAG50172.1 AF230394 mRNA Translation: AAG50173.1 AL662859 Genomic DNA No translation available. AL662871 Genomic DNA No translation available. BX000360 Genomic DNA No translation available. BX537153 Genomic DNA No translation available. BX005144 Genomic DNA No translation available. BX119924 Genomic DNA No translation available. CR759942 Genomic DNA No translation available. Z84474, Z84476 Genomic DNA Translation: CAB06480.2 Z84476, Z84474 Genomic DNA Translation: CAI19959.1 CH471081 Genomic DNA Translation: EAX03176.1 CH471081 Genomic DNA Translation: EAX03177.1 BC013580 mRNA Translation: AAH13580.1 BC066924 mRNA Translation: AAH66924.1 |
CCDSi | CCDS4654.1 [P14373-1] |
PIRi | A28101, TVHURF |
RefSeqi | NP_006501.1, NM_006510.4 [P14373-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicing, Chromosomal rearrangementSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03407 mRNA Translation: AAA36564.1 AF230393 mRNA Translation: AAG50172.1 AF230394 mRNA Translation: AAG50173.1 AL662859 Genomic DNA No translation available. AL662871 Genomic DNA No translation available. BX000360 Genomic DNA No translation available. BX537153 Genomic DNA No translation available. BX005144 Genomic DNA No translation available. BX119924 Genomic DNA No translation available. CR759942 Genomic DNA No translation available. Z84474, Z84476 Genomic DNA Translation: CAB06480.2 Z84476, Z84474 Genomic DNA Translation: CAI19959.1 CH471081 Genomic DNA Translation: EAX03176.1 CH471081 Genomic DNA Translation: EAX03177.1 BC013580 mRNA Translation: AAH13580.1 BC066924 mRNA Translation: AAH66924.1 |
CCDSi | CCDS4654.1 [P14373-1] |
PIRi | A28101, TVHURF |
RefSeqi | NP_006501.1, NM_006510.4 [P14373-1] |
3D structure databases
SMRi | P14373 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111919, 508 interactors |
CORUMi | P14373 |
IntActi | P14373, 418 interactors |
MINTi | P14373 |
STRINGi | 9606.ENSP00000366404 |
Protein family/group databases
MoonDBi | P14373, Predicted |
PTM databases
GlyGeni | P14373, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P14373 |
MetOSitei | P14373 |
PhosphoSitePlusi | P14373 |
Genetic variation databases
BioMutai | TRIM27 |
DMDMi | 132517 |
Proteomic databases
EPDi | P14373 |
jPOSTi | P14373 |
MassIVEi | P14373 |
MaxQBi | P14373 |
PaxDbi | P14373 |
PeptideAtlasi | P14373 |
PRIDEi | P14373 |
ProteomicsDBi | 53047 [P14373-1] 53048 [P14373-2] |
Protocols and materials databases
Antibodypediai | 25916, 279 antibodies from 25 providers |
DNASUi | 5987 |
Genome annotation databases
Organism-specific databases
CTDi | 5987 |
DisGeNETi | 5987 |
GeneCardsi | TRIM27 |
HGNCi | HGNC:9975, TRIM27 |
HPAi | ENSG00000204713, Low tissue specificity |
MalaCardsi | TRIM27 |
MIMi | 602165, gene |
neXtProti | NX_P14373 |
OpenTargetsi | ENSG00000204713 |
Orphaneti | 146, Differentiated thyroid carcinoma |
PharmGKBi | PA162406956 |
VEuPathDBi | HostDB:ENSG00000204713 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000158537 |
HOGENOMi | CLU_013137_6_1_1 |
InParanoidi | P14373 |
OMAi | ICCACLT |
PhylomeDBi | P14373 |
TreeFami | TF350411 |
Enzyme and pathway databases
PathwayCommonsi | P14373 |
Reactomei | R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-8948751, Regulation of PTEN stability and activity R-HSA-9635465, Suppression of apoptosis |
SignaLinki | P14373 |
SIGNORi | P14373 |
Miscellaneous databases
BioGRID-ORCSi | 5987, 10 hits in 1090 CRISPR screens |
ChiTaRSi | TRIM27, human |
GeneWikii | TRIM27 |
GenomeRNAii | 5987 |
Pharosi | P14373, Tbio |
PROi | PR:P14373 |
RNActi | P14373, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204713, Expressed in right lobe of liver and 235 other tissues |
ExpressionAtlasi | P14373, baseline and differential |
Genevisiblei | P14373, HS |
Family and domain databases
CDDi | cd15814, SPRY_PRY_TRIM27, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035791, SPRY/PRY_TRIM27 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR020457, Znf_B-box_chordata IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit |
PRINTSi | PR01406, BBOXZNFINGER PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI27_HUMAN | |
Accessioni | P14373Primary (citable) accession number: P14373 Secondary accession number(s): A2BE15 Q9UJL3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 232 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families