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Protein

Interferon regulatory factor 2

Gene

IRF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi5 – 113IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST109

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling
R-HSA-909733 Interferon alpha/beta signaling
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiP14316
SIGNORiP14316

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 2
Short name:
IRF-2
Gene namesi
Name:IRF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000168310.10
HGNCiHGNC:6117 IRF2
MIMi147576 gene
neXtProtiNX_P14316

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75K → R: Diminished acetylation by both CREBBP/p300 and PCAF. Greatly reduced enhancement of H4 promoter activity. 1 Publication1
Mutagenesisi78K → R: Greatly diminished acetylation by PCAF. Lesser loss of acetylation by CREBBP/p300. Loss of DNA binding and no enhancement of H4 promoter activity. 1 Publication1
Mutagenesisi137K → R: Some loss of sumoylation. Increases IRF2-mediation activation of ISRE and H4 promoters. Increased inhibition of IRF1-mediated transcription. Additional small loss of sumoylation; when associated with R-166. Great loss of sumoylation; when associated with R-296. Abolishes sumoylation. Greatly increased activation of ISRE and H4 promoters and further increased ability to inhibit IRF1-mediated transcription; when associated with R-166 and R-293. 1 Publication1
Mutagenesisi166K → R: Little loss of sumoylation. Increases IRF2-mediation activation of ISRE and H4 promoters. Increased inhibition of IRF1-mediated transcription. Greater loss of sumoylation; when associated with R-137. Further loss of sumoylation; when associated with R-293. Abolishes sumoylation. Greatly increased activation of ISRE and H4 promoters and further increased ability to inhibit IRF1-mediated transcription; when associated with R-137 and R-293. 1 Publication1
Mutagenesisi293K → R: Some loss of sumoylation. Increases IRF2-mediation activation of ISRE and H4 promoters. Increased inhibition of IRF1-mediated transcription. Further small loss of sumoylation; when associated with R-166. Great loss of sumoylation; when associated with R-137. Abolishes sumoylation. Greatly increased activation of ISRE and H4 promoters and further increased ability to inhibit IRF1-mediated transcription; when associated with R-166 and R-293. 1 Publication1

Organism-specific databases

DisGeNETi3660
OpenTargetsiENSG00000168310
PharmGKBiPA29916

Polymorphism and mutation databases

BioMutaiIRF2
DMDMi20141499

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001545491 – 349Interferon regulatory factor 2Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei75N6-acetyllysine1 Publication1
Modified residuei78N6-acetyllysine1 Publication1
Cross-linki137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki166Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei225PhosphoserineCombined sources1
Cross-linki260Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.1 Publication
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP14316
PaxDbiP14316
PeptideAtlasiP14316
PRIDEiP14316
ProteomicsDBi53043
53044 [P14316-2]

PTM databases

iPTMnetiP14316
PhosphoSitePlusiP14316

Expressioni

Tissue specificityi

Expressed throughout the epithelium of the colon. Also expressed in lamina propria.1 Publication

Inductioni

By viruses and IFN.

Gene expression databases

BgeeiENSG00000168310 Expressed in 201 organ(s), highest expression level in leukocyte
CleanExiHS_IRF2
ExpressionAtlasiP14316 baseline and differential
GenevisibleiP14316 HS

Organism-specific databases

HPAiCAB032306
HPA030813
HPA057327

Interactioni

Subunit structurei

Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi109868, 29 interactors
ELMiP14316
IntActiP14316, 14 interactors
MINTiP14316
STRINGi9606.ENSP00000377218

Structurei

3D structure databases

ProteinModelPortaliP14316
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGY9 Eukaryota
ENOG411152R LUCA
GeneTreeiENSGT00760000119093
HOGENOMiHOG000037937
HOVERGENiHBG003455
InParanoidiP14316
KOiK10153
OMAiSWPPFPD
OrthoDBiEOG091G0CB8
PhylomeDBiP14316
TreeFamiTF328512

Family and domain databases

CDDicd00103 IRF, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR019817 Interferon_reg_fac_CS
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR017431 IRF1/IRF2
IPR031218 IRF2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11949 PTHR11949, 1 hit
PTHR11949:SF22 PTHR11949:SF22, 1 hit
PfamiView protein in Pfam
PF00605 IRF, 1 hit
PIRSFiPIRSF038196 IFN_RF1/2, 1 hit
PRINTSiPR00267 INTFRNREGFCT
SMARTiView protein in SMART
SM00348 IRF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00601 IRF_1, 1 hit
PS51507 IRF_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P14316-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK
60 70 80 90 100
DAPLFRNWAI HTGKHQPGVD KPDPKTWKAN FRCAMNSLPD IEEVKDKSIK
110 120 130 140 150
KGNNAFRVYR MLPLSERPSK KGKKPKTEKE DKVKHIKQEP VESSLGLSNG
160 170 180 190 200
VSDLSPEYAV LTSTIKNEVD STVNIIVVGQ SHLDSNIENQ EIVTNPPDIC
210 220 230 240 250
QVVEVTTESD EQPVSMSELY PLQISPVSSY AESETTDSVP SDEESAEGRP
260 270 280 290 300
HWRKRNIEGK QYLSNMGTRG SYLLPGMASF VTSNKPDLQV TIKEESNPVP
310 320 330 340
YNSSWPPFQD LPLSSSMTPA SSSSRPDRET RASVIKKTSD ITQARVKSC
Length:349
Mass (Da):39,354
Last modified:February 11, 2002 - v2
Checksum:i6298341652466560
GO
Isoform 2 (identifier: P14316-2) [UniParc]FASTAAdd to basket
Also known as: IRF-2s, IRF-2[S]

The sequence of this isoform differs from the canonical sequence as follows:
     177-178: Missing.

Note: Unable to bind to IRF2BP1 and IRF2BP2 corepressors and cannot mediate repression.
Show »
Length:347
Mass (Da):39,156
Checksum:iEE67F41029BFD6BD
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K4DIA5K4DIA5_HUMAN
Interferon regulatory factor 2
IRF2
176Annotation score:
D6R9N5D6R9N5_HUMAN
Interferon regulatory factor 2
IRF2
137Annotation score:
D6RB08D6RB08_HUMAN
Interferon regulatory factor 2
IRF2
160Annotation score:
H0Y956H0Y956_HUMAN
Interferon regulatory factor 2
IRF2
113Annotation score:
D6RED1D6RED1_HUMAN
Interferon regulatory factor 2
IRF2
83Annotation score:
K4DIA4K4DIA4_HUMAN
Interferon regulatory factor 2
IRF2
196Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58W → R in CAA34073 (PubMed:2813069).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043965177 – 178Missing in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15949 mRNA Translation: CAA34073.1
BT007264 mRNA Translation: AAP35928.1
AK312953 mRNA Translation: BAG35793.1
CR457077 mRNA Translation: CAG33358.1
AC099343 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX04677.1
CH471056 Genomic DNA Translation: EAX04678.1
CH471056 Genomic DNA Translation: EAX04680.1
BC015803 mRNA Translation: AAH15803.1
CCDSiCCDS3835.1 [P14316-1]
PIRiA53340
RefSeqiNP_002190.2, NM_002199.3 [P14316-1]
UniGeneiHs.654566

Genome annotation databases

EnsembliENST00000393593; ENSP00000377218; ENSG00000168310 [P14316-1]
GeneIDi3660
KEGGihsa:3660
UCSCiuc003iwf.5 human [P14316-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15949 mRNA Translation: CAA34073.1
BT007264 mRNA Translation: AAP35928.1
AK312953 mRNA Translation: BAG35793.1
CR457077 mRNA Translation: CAG33358.1
AC099343 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX04677.1
CH471056 Genomic DNA Translation: EAX04678.1
CH471056 Genomic DNA Translation: EAX04680.1
BC015803 mRNA Translation: AAH15803.1
CCDSiCCDS3835.1 [P14316-1]
PIRiA53340
RefSeqiNP_002190.2, NM_002199.3 [P14316-1]
UniGeneiHs.654566

3D structure databases

ProteinModelPortaliP14316
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109868, 29 interactors
ELMiP14316
IntActiP14316, 14 interactors
MINTiP14316
STRINGi9606.ENSP00000377218

PTM databases

iPTMnetiP14316
PhosphoSitePlusiP14316

Polymorphism and mutation databases

BioMutaiIRF2
DMDMi20141499

Proteomic databases

EPDiP14316
PaxDbiP14316
PeptideAtlasiP14316
PRIDEiP14316
ProteomicsDBi53043
53044 [P14316-2]

Protocols and materials databases

DNASUi3660
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393593; ENSP00000377218; ENSG00000168310 [P14316-1]
GeneIDi3660
KEGGihsa:3660
UCSCiuc003iwf.5 human [P14316-1]

Organism-specific databases

CTDi3660
DisGeNETi3660
EuPathDBiHostDB:ENSG00000168310.10
GeneCardsiIRF2
H-InvDBiHIX0004671
HGNCiHGNC:6117 IRF2
HPAiCAB032306
HPA030813
HPA057327
MIMi147576 gene
neXtProtiNX_P14316
OpenTargetsiENSG00000168310
PharmGKBiPA29916
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGY9 Eukaryota
ENOG411152R LUCA
GeneTreeiENSGT00760000119093
HOGENOMiHOG000037937
HOVERGENiHBG003455
InParanoidiP14316
KOiK10153
OMAiSWPPFPD
OrthoDBiEOG091G0CB8
PhylomeDBiP14316
TreeFamiTF328512

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling
R-HSA-909733 Interferon alpha/beta signaling
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiP14316
SIGNORiP14316

Miscellaneous databases

ChiTaRSiIRF2 human
GeneWikiiIRF2
GenomeRNAii3660
PROiPR:P14316
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168310 Expressed in 201 organ(s), highest expression level in leukocyte
CleanExiHS_IRF2
ExpressionAtlasiP14316 baseline and differential
GenevisibleiP14316 HS

Family and domain databases

CDDicd00103 IRF, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR019817 Interferon_reg_fac_CS
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR017431 IRF1/IRF2
IPR031218 IRF2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11949 PTHR11949, 1 hit
PTHR11949:SF22 PTHR11949:SF22, 1 hit
PfamiView protein in Pfam
PF00605 IRF, 1 hit
PIRSFiPIRSF038196 IFN_RF1/2, 1 hit
PRINTSiPR00267 INTFRNREGFCT
SMARTiView protein in SMART
SM00348 IRF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00601 IRF_1, 1 hit
PS51507 IRF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIRF2_HUMAN
AccessioniPrimary (citable) accession number: P14316
Secondary accession number(s): D6RCK5
, H0Y8S3, Q6IAS7, Q96B99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 11, 2002
Last modified: November 7, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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