UniProtKB - P14295 (DHL2_WEICO)
Protein
L-2-hydroxyisocaproate dehydrogenase
Gene
N/A
Organism
Weissella confusa (Lactobacillus confusus)
Status
Functioni
Catalyzes the NADP dependent reversible and stereospecific interconversion between 2-ketocarboxylic acids and L-2-hydroxy-carboxylic acids. 2-ketoacids with medium chain length (five to six C-atoms) are the best substrates.
Miscellaneous
Can be applied in an industrial process for the production of L-amino acid.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | SubstrateBy similarity | 1 | |
Binding sitei | 124 | NAD or substrateBy similarity | 1 | |
Binding sitei | 155 | SubstrateBy similarity | 1 | |
Active sitei | 179 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 55 | NAD1 PublicationAdd BLAST | 22 |
GO - Molecular functioni
- L-lactate dehydrogenase activity Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- carboxylic acid metabolic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.337, 2857 |
SABIO-RKi | P14295 |
Names & Taxonomyi
Protein namesi | |
Organismi | Weissella confusa (Lactobacillus confusus) |
Taxonomic identifieri | 1583 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Leuconostocaceae › Weissella |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000168501 | 2 – 310 | L-2-hydroxyisocaproate dehydrogenaseAdd BLAST | 309 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14295 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14295 |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR018177, L-lactate_DH_AS IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
PRINTSi | PR00086, LLDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS00064, L_LDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P14295-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARKIGIIGL GNVGAAVAHG LIAQGVADDY VFIDANEAKV KADQIDFQDA
60 70 80 90 100
MANLEAHGNI VINDWAALAD ADVVISTLGN IKLQQDNPTG DRFAELKFTS
110 120 130 140 150
SMVQSVGTNL KESGFHGVLV VISNPVDVIT ALFQHVTGFP AHKVIGTGTL
160 170 180 190 200
LDTARMQRAV GEAFDLDPRS VSGYNLGEHG NSQFVAWSTV RVMGQPIVTL
210 220 230 240 250
ADAGDIDLAA IEEEARKGGF TVLNGKGYTS YGVATSAIRI AKAVMADAHA
260 270 280 290 300
ELVVSNRRDD MGMYLSYPAI IGRDGVLAET TLDLTTDEQE KLLQSRDYIQ
310
QRFDEIVDTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31425 Genomic DNA Translation: AAA88213.1 |
PIRi | JQ0114 |
RefSeqi | WP_003607654.1, NZ_QSBT01000002.1 |
Genome annotation databases
GeneIDi | 57979094 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31425 Genomic DNA Translation: AAA88213.1 |
PIRi | JQ0114 |
RefSeqi | WP_003607654.1, NZ_QSBT01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HYH | X-ray | 2.20 | A/B/C/D | 2-310 | [»] | |
SMRi | P14295 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
GeneIDi | 57979094 |
Enzyme and pathway databases
BRENDAi | 1.1.1.337, 2857 |
SABIO-RKi | P14295 |
Miscellaneous databases
EvolutionaryTracei | P14295 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR018177, L-lactate_DH_AS IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
PRINTSi | PR00086, LLDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS00064, L_LDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHL2_WEICO | |
Accessioni | P14295Primary (citable) accession number: P14295 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 115 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families