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Protein

DNA mismatch repair protein PMS1

Gene

PMS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for DNA mismatch repair (MMR), correcting base-base mismatches and insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLalpha heterodimer that forms a ternary complex with the MutS heterodimers, which initially recognize the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in maintaining the genetic stability of simple sequence repeats and in the repair of heteroduplex sites present in meiotic recombination intermediates.3 Publications

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Kineticsi

  1. KM=1.5 mM for ATP1 Publication

    GO - Molecular functioni

    • ATPase activity Source: SGD
    • ATP binding Source: SGD
    • mismatched DNA binding Source: InterPro
    • single-stranded DNA binding Source: GO_Central

    GO - Biological processi

    Keywordsi

    Biological processDNA damage, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33111-MONOMER
    ReactomeiR-SCE-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA mismatch repair protein PMS1
    Alternative name(s):
    Postmeiotic segregation protein 1
    Gene namesi
    Name:PMS1
    Ordered Locus Names:YNL082W
    ORF Names:N2317
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIV

    Organism-specific databases

    SGDiS000005026 PMS1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi30E → A: Reduces ATPase activity by 62%. Displays 60-fold increase in spontaneous mutation accumulation. 2 Publications1
    Mutagenesisi34N → A: Reduces ATPase activity by 84%. Displays 11000-fold increase in spontaneous mutation accumulation. 1 Publication1
    Mutagenesisi95F → A: Displays an increase in spontaneous mutation accumulation. Does not impair heterodimer formation. 1 Publication1
    Mutagenesisi97G → A: Displays an increase in spontaneous mutation accumulation. 1 Publication1
    Mutagenesisi297K → E: Displays a 60-fold increase in spontaneous mutation accumulation. 1 Publication1
    Mutagenesisi851G → E: Confers a strong defect in the repair of primer strand-specific 1-bp loops during DNA replication, but not during meoitic recombination. Does not impair heterodimer formation. 1 Publication1
    Mutagenesisi857H → R: Confers a strong defect in the repair of primer strand-specific 1-bp loops during DNA replication, but not during meoitic recombination. Does not impair heterodimer formation. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002455711 – 873DNA mismatch repair protein PMS1Add BLAST873

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei393PhosphoserineCombined sources1
    Modified residuei566PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP14242
    PaxDbiP14242
    PRIDEiP14242

    PTM databases

    iPTMnetiP14242

    Interactioni

    Subunit structurei

    Heterodimer of MLH1 and PMS1, called MutLalpha, which is the major MMR MutL activity correcting base-base mismatches as well as IDLs. The heterodimer binds double strand DNA independently of a mismatch with positive cooperativity and has more than one DNA binding site. Forms a ternary complex with either the MSH2-MSH6 (MutSalpha) or the MSH2-MSH3 heterodimer (MutSbeta), which recognize and bind to mismatch DNA. Ternary complex formation is promoted by ATP binding.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MLH1P3892012EBI-13561,EBI-11003

    Protein-protein interaction databases

    BioGridi35741, 240 interactors
    ComplexPortaliCPX-1666 MutLalpha complex
    DIPiDIP-2416N
    IntActiP14242, 8 interactors
    MINTiP14242
    STRINGi4932.YNL082W

    Structurei

    Secondary structure

    1873
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP14242
    SMRiP14242
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14242

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 357DNA- and ATP-bindingAdd BLAST357
    Regioni661 – 873Interaction with MLH1Add BLAST213

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00530000063289
    InParanoidiP14242
    KOiK10858
    OMAiFFFINQR
    OrthoDBiEOG092C22BC

    Family and domain databases

    CDDicd00075 HATPase_c, 1 hit
    Gene3Di3.30.230.10, 1 hit
    3.30.565.10, 1 hit
    InterProiView protein in InterPro
    IPR014762 DNA_mismatch_repair_CS
    IPR002099 DNA_mismatch_repair_N
    IPR013507 DNA_mismatch_S5_2-like
    IPR003594 HATPase_C
    IPR036890 HATPase_C_sf
    IPR038973 MutL/Mlh/Pms
    IPR014790 MutL_C
    IPR037198 MutL_C_sf
    IPR020568 Ribosomal_S5_D2-typ_fold
    IPR014721 Ribosomal_S5_D2-typ_fold_subgr
    PANTHERiPTHR10073 PTHR10073, 1 hit
    PfamiView protein in Pfam
    PF01119 DNA_mis_repair, 1 hit
    PF08676 MutL_C, 1 hit
    SMARTiView protein in SMART
    SM01340 DNA_mis_repair, 1 hit
    SM00853 MutL_C, 1 hit
    SUPFAMiSSF118116 SSF118116, 1 hit
    SSF54211 SSF54211, 1 hit
    SSF55874 SSF55874, 1 hit
    TIGRFAMsiTIGR00585 mutl, 1 hit
    PROSITEiView protein in PROSITE
    PS00058 DNA_MISMATCH_REPAIR_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P14242-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTQIHQINDI DVHRITSGQV ITDLTTAVKE LVDNSIDANA NQIEIIFKDY
    60 70 80 90 100
    GLESIECSDN GDGIDPSNYE FLALKHYTSK IAKFQDVAKV QTLGFRGEAL
    110 120 130 140 150
    SSLCGIAKLS VITTTSPPKA DKLEYDMVGH ITSKTTTSRN KGTTVLVSQL
    160 170 180 190 200
    FHNLPVRQKE FSKTFKRQFT KCLTVIQGYA IINAAIKFSV WNITPKGKKN
    210 220 230 240 250
    LILSTMRNSS MRKNISSVFG AGGMRGLEEV DLVLDLNPFK NRMLGKYTDD
    260 270 280 290 300
    PDFLDLDYKI RVKGYISQNS FGCGRNSKDR QFIYVNKRPV EYSTLLKCCN
    310 320 330 340 350
    EVYKTFNNVQ FPAVFLNLEL PMSLIDVNVT PDKRVILLHN ERAVIDIFKT
    360 370 380 390 400
    TLSDYYNRQE LALPKRMCSQ SEQQAQKRLK TEVFDDRSTT HESDNENYHT
    410 420 430 440 450
    ARSESNQSNH AHFNSTTGVI DKSNGTELTS VMDGNYTNVT DVIGSECEVS
    460 470 480 490 500
    VDSSVVLDEG NSSTPTKKLP SIKTDSQNLS DLNLNNFSNP EFQNITSPDK
    510 520 530 540 550
    ARSLEKVVEE PVYFDIDGEK FQEKAVLSQA DGLVFVDNEC HEHTNDCCHQ
    560 570 580 590 600
    ERRGSTDTEQ DDEADSIYAE IEPVEINVRT PLKNSRKSIS KDNYRSLSDG
    610 620 630 640 650
    LTHRKFEDEI LEYNLSTKNF KEISKNGKQM SSIISKRKSE AQENIIKNKD
    660 670 680 690 700
    ELEDFEQGEK YLTLTVSKND FKKMEVVGQF NLGFIIVTRK VDNKYDLFIV
    710 720 730 740 750
    DQHASDEKYN FETLQAVTVF KSQKLIIPQP VELSVIDELV VLDNLPVFEK
    760 770 780 790 800
    NGFKLKIDEE EEFGSRVKLL SLPTSKQTLF DLGDFNELIH LIKEDGGLRR
    810 820 830 840 850
    DNIRCSKIRS MFAMRACRSS IMIGKPLNKK TMTRVVHNLS ELDKPWNCPH
    860 870
    GRPTMRHLME LRDWSSFSKD YEI
    Length:873
    Mass (Da):99,355
    Last modified:July 11, 2006 - v3
    Checksum:i2E0FFBC59B718854
    GO

    Sequence cautioni

    The sequence AAA34885 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00521 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00533 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00545 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00551 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00563 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00569 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA60176 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA61428 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA95956 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA95957 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti695Y → S in AAA34885 (PubMed:2676974).Curated1
    Sequence conflicti861L → I in AAA34885 (PubMed:2676974).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti41N → S in strain: SK1 and YJM421. 2 Publications1
    Natural varianti112I → T in strain: SK1 and YJM421. 2 Publications1
    Natural varianti384F → V in strain: SK1, YJM320, YJM339 and YJM421. 2 Publications1
    Natural varianti392E → V in strain: YJM320. 1 Publication1
    Natural varianti400T → S in strain: SK1, YJM320, YJM339 and YJM421. 2 Publications1
    Natural varianti401A → S in strain: YJM320 and YJM421. 1 Publication1
    Natural varianti416T → TCEGT in strain: SK1, YJM320, YJM339 and YJM421. 1
    Natural varianti458D → Y in strain: EAY1068. 1 Publication1
    Natural varianti475D → N in strain: YJM339. 1 Publication1
    Natural varianti513Y → F in strain: SK1. 1 Publication1
    Natural varianti564A → V in strain: YJM320. 1 Publication1
    Natural varianti768K → R in strain: YJM320. 1 Publication1
    Natural varianti818R → K in strain: SK1 and YJM320; forms a non-functional heterodimer with MHL1 from strain S288c, resulting in an accumulation of mutations in spore progeny of crosses between these strains. 2 Publications1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M29688 Genomic DNA Translation: AAA34885.1 Different initiation.
    AF458969 Genomic DNA Translation: AAM00521.1 Different initiation.
    AF458971 Genomic DNA Translation: AAM00533.1 Different initiation.
    AF458973 Genomic DNA Translation: AAM00545.1 Different initiation.
    AF458974 Genomic DNA Translation: AAM00551.1 Different initiation.
    AF458976 Genomic DNA Translation: AAM00563.1 Different initiation.
    AF458977 Genomic DNA Translation: AAM00569.1 Different initiation.
    DQ115393 Genomic DNA Translation: AAZ22526.1
    DQ356628 Genomic DNA Translation: ABC86932.1
    DQ356629 Genomic DNA Translation: ABC86933.1
    DQ356630 Genomic DNA Translation: ABC86934.1
    DQ356631 Genomic DNA Translation: ABC86935.1
    DQ356632 Genomic DNA Translation: ABC86936.1
    X86470 Genomic DNA Translation: CAA60176.1 Different initiation.
    Z71357 Genomic DNA Translation: CAA95956.1 Different initiation.
    Z71358 Genomic DNA Translation: CAA95957.1 Different initiation.
    X89016 Genomic DNA Translation: CAA61428.1 Different initiation.
    BK006947 Genomic DNA Translation: DAA10463.1
    PIRiS53896
    RefSeqiNP_014317.4, NM_001182920.3

    Genome annotation databases

    EnsemblFungiiYNL082W; YNL082W; YNL082W
    GeneIDi855642
    KEGGisce:YNL082W

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M29688 Genomic DNA Translation: AAA34885.1 Different initiation.
    AF458969 Genomic DNA Translation: AAM00521.1 Different initiation.
    AF458971 Genomic DNA Translation: AAM00533.1 Different initiation.
    AF458973 Genomic DNA Translation: AAM00545.1 Different initiation.
    AF458974 Genomic DNA Translation: AAM00551.1 Different initiation.
    AF458976 Genomic DNA Translation: AAM00563.1 Different initiation.
    AF458977 Genomic DNA Translation: AAM00569.1 Different initiation.
    DQ115393 Genomic DNA Translation: AAZ22526.1
    DQ356628 Genomic DNA Translation: ABC86932.1
    DQ356629 Genomic DNA Translation: ABC86933.1
    DQ356630 Genomic DNA Translation: ABC86934.1
    DQ356631 Genomic DNA Translation: ABC86935.1
    DQ356632 Genomic DNA Translation: ABC86936.1
    X86470 Genomic DNA Translation: CAA60176.1 Different initiation.
    Z71357 Genomic DNA Translation: CAA95956.1 Different initiation.
    Z71358 Genomic DNA Translation: CAA95957.1 Different initiation.
    X89016 Genomic DNA Translation: CAA61428.1 Different initiation.
    BK006947 Genomic DNA Translation: DAA10463.1
    PIRiS53896
    RefSeqiNP_014317.4, NM_001182920.3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3H4LX-ray2.50A/B1-365[»]
    4E4WX-ray2.50B635-873[»]
    4FMNX-ray2.69B635-873[»]
    4FMOX-ray3.04B635-873[»]
    ProteinModelPortaliP14242
    SMRiP14242
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35741, 240 interactors
    ComplexPortaliCPX-1666 MutLalpha complex
    DIPiDIP-2416N
    IntActiP14242, 8 interactors
    MINTiP14242
    STRINGi4932.YNL082W

    PTM databases

    iPTMnetiP14242

    Proteomic databases

    MaxQBiP14242
    PaxDbiP14242
    PRIDEiP14242

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYNL082W; YNL082W; YNL082W
    GeneIDi855642
    KEGGisce:YNL082W

    Organism-specific databases

    SGDiS000005026 PMS1

    Phylogenomic databases

    GeneTreeiENSGT00530000063289
    InParanoidiP14242
    KOiK10858
    OMAiFFFINQR
    OrthoDBiEOG092C22BC

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33111-MONOMER
    ReactomeiR-SCE-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)

    Miscellaneous databases

    EvolutionaryTraceiP14242
    PROiPR:P14242

    Family and domain databases

    CDDicd00075 HATPase_c, 1 hit
    Gene3Di3.30.230.10, 1 hit
    3.30.565.10, 1 hit
    InterProiView protein in InterPro
    IPR014762 DNA_mismatch_repair_CS
    IPR002099 DNA_mismatch_repair_N
    IPR013507 DNA_mismatch_S5_2-like
    IPR003594 HATPase_C
    IPR036890 HATPase_C_sf
    IPR038973 MutL/Mlh/Pms
    IPR014790 MutL_C
    IPR037198 MutL_C_sf
    IPR020568 Ribosomal_S5_D2-typ_fold
    IPR014721 Ribosomal_S5_D2-typ_fold_subgr
    PANTHERiPTHR10073 PTHR10073, 1 hit
    PfamiView protein in Pfam
    PF01119 DNA_mis_repair, 1 hit
    PF08676 MutL_C, 1 hit
    SMARTiView protein in SMART
    SM01340 DNA_mis_repair, 1 hit
    SM00853 MutL_C, 1 hit
    SUPFAMiSSF118116 SSF118116, 1 hit
    SSF54211 SSF54211, 1 hit
    SSF55874 SSF55874, 1 hit
    TIGRFAMsiTIGR00585 mutl, 1 hit
    PROSITEiView protein in PROSITE
    PS00058 DNA_MISMATCH_REPAIR_1, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPMS1_YEAST
    AccessioniPrimary (citable) accession number: P14242
    Secondary accession number(s): D6W197
    , Q2I044, Q2I045, Q45TY4, Q8TG48, Q8TG50, Q8TG54, Q8TG57
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: July 11, 2006
    Last modified: November 7, 2018
    This is version 185 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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