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Protein

Type-2 restriction enzyme SmaI

Gene

smaIR

Organism
Serratia marcescens
Status
Reviewed-Annotation score: -Protein predictedi

Functioni

Recognizes the double-stranded sequence CCCGGG and cleaves after C-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium

Enzyme and pathway databases

BRENDAi3.1.21.4 5690

Protein family/group databases

REBASEi1704 SmaI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SmaI (EC:3.1.21.4)
Short name:
R.SmaI
Alternative name(s):
Endonuclease SmaI
Type II restriction enzyme SmaI
Gene namesi
Name:smaIR
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773621 – 247Type-2 restriction enzyme SmaIAdd BLAST247

Sequencei

Sequence statusi: Complete.

P14229-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRDDQLFTL WGKLNDRQKD NFLKWMKAFD VEKTYQKTSG DIFNDDFFDI
60 70 80 90 100
FGDRLITHHF SSTQALTKTL FEHAFNDSLN ESGVISSLAE SRTNPGHDIT
110 120 130 140 150
IDSIKVALKT EAAKNISKSY IHVSKWMELG KGEWILELLL ERFLEHLENY
160 170 180 190 200
ERIFTLRYFK ISEYKFSYQL VEIPKSLLLE AKNAKLEIMS GSKQSPKPGY
210 220 230 240
GYVLDENENK KFSLYFDGGA ERKLQIKHLN LEHCIVHGVW DFILPPP
Length:247
Mass (Da):28,782
Last modified:January 1, 1990 - v1
Checksum:iDBE6CD66362DCF93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16458 Genomic DNA Translation: CAA34478.1
M98769 Genomic DNA Translation: AAA26569.1
PIRiS06035

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16458 Genomic DNA Translation: CAA34478.1
M98769 Genomic DNA Translation: AAA26569.1
PIRiS06035

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1704 SmaI

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.21.4 5690

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiT2SM_SERMA
AccessioniPrimary (citable) accession number: P14229
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 12, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
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Main funding by: National Institutes of Health

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