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Protein

60S ribosomal protein L30

Gene

RPL30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 59300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • pre-mRNA 5'-splice site binding Source: SGD
  • RNA binding Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: GO_Central
  • negative regulation of mRNA splicing, via spliceosome Source: SGD
  • rRNA processing Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30546-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Protein family/group databases

MoonProtiP14120

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L301 Publication
Alternative name(s):
L32
Large ribosomal subunit protein eL301 Publication
RP73
YL38
Gene namesi
Name:RPL301 Publication
Synonyms:RPL32
Ordered Locus Names:YGL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL030W
SGDiS000002998 RPL30

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001461432 – 10560S ribosomal protein L30Add BLAST104

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki83Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP14120
PaxDbiP14120
PRIDEiP14120
TopDownProteomicsiP14120

PTM databases

iPTMnetiP14120

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33216, 93 interactors
ComplexPortaliCPX-1601 60S cytosolic large ribosomal subunit
DIPiDIP-1891N
IntActiP14120, 94 interactors
MINTiP14120
STRINGi4932.YGL030W

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Beta strandi21 – 25Combined sources5
Helixi26 – 35Combined sources10
Beta strandi40 – 45Combined sources6
Helixi53 – 62Combined sources10
Beta strandi67 – 70Combined sources4
Helixi79 – 87Combined sources9
Beta strandi88 – 94Combined sources7
Helixi100 – 104Combined sources5

3D structure databases

ProteinModelPortaliP14120
SMRiP14120
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14120

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000012138
HOGENOMiHOG000055224
InParanoidiP14120
KOiK02908
OMAiYEFEGTS
OrthoDBiEOG092C5LQM

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
InterProiView protein in InterPro
IPR039109 L30/L30e/YlxQ
IPR029064 L30e-like
IPR000231 Ribosomal_L30e
IPR022991 Ribosomal_L30e_CS
IPR004038 Ribosomal_L7Ae/L30e/S12e/Gad45
PANTHERiPTHR11449 PTHR11449, 1 hit
PTHR11449:SF1 PTHR11449:SF1, 1 hit
PfamiView protein in Pfam
PF01248 Ribosomal_L7Ae, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
PROSITEiView protein in PROSITE
PS00709 RIBOSOMAL_L30E_1, 1 hit
PS00993 RIBOSOMAL_L30E_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV
60 70 80 90 100
LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI

LTTLA
Length:105
Mass (Da):11,415
Last modified:January 23, 2007 - v3
Checksum:i963C973A02E9C8E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03457 Genomic DNA Translation: AAA35005.1
Z72552 Genomic DNA Translation: CAA96731.1
BK006941 Genomic DNA Translation: DAA08069.1
PIRiA29779 R6BY30
RefSeqiNP_011485.1, NM_001180895.1

Genome annotation databases

EnsemblFungiiYGL030W; YGL030W; YGL030W
GeneIDi852853
KEGGisce:YGL030W

Similar proteinsi

Entry informationi

Entry nameiRL30_YEAST
AccessioniPrimary (citable) accession number: P14120
Secondary accession number(s): D6VUA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 182 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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