UniProtKB - P14100 (PDE1A_BOVIN)
Protein
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Gene
PDE1A
Organism
Bos taurus (Bovine)
Status
Functioni
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).By similarity
Catalytic activityi
- EC:3.1.4.17
Cofactori
a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity
Activity regulationi
Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 219 | Proton donorBy similarity | 1 | |
Metal bindingi | 223 | Divalent metal cation 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 259 | Divalent metal cation 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 260 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 260 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 366 | Divalent metal cation 1By similarity | 1 |
GO - Molecular functioni
- 3',5'-cyclic-nucleotide phosphodiesterase activity Source: GO_Central
- calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
- calmodulin binding Source: UniProtKB-KW
- calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase |
Ligand | cAMP, cGMP, Metal-binding |
Enzyme and pathway databases
Reactomei | R-BTA-111957, Cam-PDE 1 activation R-BTA-418457, cGMP effects R-BTA-418555, G alpha (s) signalling events |
SABIO-RKi | P14100 |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A (EC:3.1.4.17)Short name: Cam-PDE 1A Alternative name(s): 61 kDa Cam-PDE |
Gene namesi | Name:PDE1A |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:32671, PDE1A |
Subcellular locationi
Other locations
- neuronal cell body Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198784 | 1 – 530 | Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1AAdd BLAST | 530 |
Proteomic databases
PaxDbi | P14100 |
PTM databases
iPTMneti | P14100 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000012100, Expressed in brain and 18 other tissues |
Interactioni
Subunit structurei
Homodimer.
Interacts with YWHAZ (By similarity).
By similarityBinary interactionsi
Hide detailsP14100
With | #Exp. | IntAct |
---|---|---|
NCALD [P61602] | 2 | EBI-907809,EBI-908133 |
Hpca [P84076] from Rattus norvegicus. | 2 | EBI-907809,EBI-908193 |
Ncs1 [P62168] from Rattus norvegicus. | 2 | EBI-907809,EBI-907774 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 159260, 2 interactors |
IntActi | P14100, 3 interactors |
STRINGi | 9913.ENSBTAP00000051204 |
Chemistry databases
BindingDBi | P14100 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 142 – 508 | PDEasePROSITE-ProRule annotationAdd BLAST | 367 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 24 – 44 | Calmodulin-bindingAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3688, Eukaryota |
GeneTreei | ENSGT00940000157043 |
HOGENOMi | CLU_005940_1_3_1 |
InParanoidi | P14100 |
OMAi | MQFPPDV |
OrthoDBi | 904682at2759 |
TreeFami | TF314638 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS IPR013706, PDEase_N |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit PF08499, PDEase_I_N, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 2 (identifier: P14100-1) [UniParc]FASTAAdd to basket
Also known as: PDE1A2
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSTATETEE LENTTFKYLI GEQTEKMWQR LKGILRCLVK QLEKGDVNVI
60 70 80 90 100
DLKKNIEYAA SVLEAVYIDE TRRLLDTDDE LSDIQSDSVP SEVRDWLAST
110 120 130 140 150
FTRKMGMMKK KSEEKPRFRS IVHVVQAGIF VERMYRKSYH MVGLAYPEAV
160 170 180 190 200
IVTLKDVDKW SFDVFALNEA SGEHSLKFMI YELFTRYDLI NRFKIPVSCL
210 220 230 240 250
IAFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI MHWLTELEIL
260 270 280 290 300
AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM
310 320 330 340 350
QEEEMNVLIN LSKDDWRDLR NLVIEMVLST DMSGHFQQIK NIRNSLQQPE
360 370 380 390 400
GLDKAKTMSL ILHAADISHP AKSWKLHHRW TMALMEEFFL QGDKEAELGL
410 420 430 440 450
PFSPLCDRKS TMVAQSQIGF IDFIVEPTFS LLTDSTEKII IPLIEEDSKT
460 470 480 490 500
KTPSYGASRR SNMKGTTNDG TYSPDYSLAS VDLKSFKNSL VDIIQQNKER
510 520 530
WKELAAQGEP DPHKNSDLVN AEEKHAETHS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 237 | H → G AA sequence (PubMed:3025833).Curated | 1 | |
Sequence conflicti | 321 | N → W AA sequence (PubMed:3025833).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004546 | 1 – 34 | MGSTA…RLKGI → MDDHVTIRRKHLQRPIFR in isoform 1. 2 PublicationsAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90358 mRNA Translation: AAA74560.1 L34069 mRNA Translation: AAA92555.1 BC123449 mRNA Translation: AAI23450.1 |
PIRi | A45334 |
RefSeqi | NP_776839.1, NM_174414.3 [P14100-2] XP_010800147.1, XM_010801845.1 [P14100-1] |
Genome annotation databases
Ensembli | ENSBTAT00000016060; ENSBTAP00000016060; ENSBTAG00000012100 [P14100-2] ENSBTAT00000052318; ENSBTAP00000051204; ENSBTAG00000012100 [P14100-1] |
GeneIDi | 281969 |
KEGGi | bta:281969 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90358 mRNA Translation: AAA74560.1 L34069 mRNA Translation: AAA92555.1 BC123449 mRNA Translation: AAI23450.1 |
PIRi | A45334 |
RefSeqi | NP_776839.1, NM_174414.3 [P14100-2] XP_010800147.1, XM_010801845.1 [P14100-1] |
3D structure databases
SMRi | P14100 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 159260, 2 interactors |
IntActi | P14100, 3 interactors |
STRINGi | 9913.ENSBTAP00000051204 |
Chemistry databases
BindingDBi | P14100 |
ChEMBLi | CHEMBL3774 |
DrugCentrali | P14100 |
PTM databases
iPTMneti | P14100 |
Proteomic databases
PaxDbi | P14100 |
Genome annotation databases
Ensembli | ENSBTAT00000016060; ENSBTAP00000016060; ENSBTAG00000012100 [P14100-2] ENSBTAT00000052318; ENSBTAP00000051204; ENSBTAG00000012100 [P14100-1] |
GeneIDi | 281969 |
KEGGi | bta:281969 |
Organism-specific databases
CTDi | 5136 |
VGNCi | VGNC:32671, PDE1A |
Phylogenomic databases
eggNOGi | KOG3688, Eukaryota |
GeneTreei | ENSGT00940000157043 |
HOGENOMi | CLU_005940_1_3_1 |
InParanoidi | P14100 |
OMAi | MQFPPDV |
OrthoDBi | 904682at2759 |
TreeFami | TF314638 |
Enzyme and pathway databases
Reactomei | R-BTA-111957, Cam-PDE 1 activation R-BTA-418457, cGMP effects R-BTA-418555, G alpha (s) signalling events |
SABIO-RKi | P14100 |
Gene expression databases
Bgeei | ENSBTAG00000012100, Expressed in brain and 18 other tissues |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS IPR013706, PDEase_N |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit PF08499, PDEase_I_N, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDE1A_BOVIN | |
Accessioni | P14100Primary (citable) accession number: P14100 Secondary accession number(s): Q08E30, Q28063 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families