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Protein

Cathepsin E

Gene

CTSE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.1 Publication

Catalytic activityi

Similar to cathepsin D, but slightly broader specificity.2 Publications

Kineticsi

  1. KM=0.06 mM for hemoglobin1 Publication
  2. KM=0.13 mM for Pro-Pro-Thr-Ile-Phe-Phe(4-NO2)-Arg-Leu1 Publication
  3. KM=0.04 mM for Lys-Pro-Ile-Glu-Phe-Phe(4-NO2)-Arg-Leu1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei96PROSITE-ProRule annotation1
    Active sitei281PROSITE-ProRule annotation1

    GO - Molecular functioni

    • aspartic-type endopeptidase activity Source: UniProtKB
    • protein homodimerization activity Source: Ensembl

    GO - Biological processi

    Keywordsi

    Molecular functionAspartyl protease, Hydrolase, Protease

    Enzyme and pathway databases

    BRENDAi3.4.23.34 2681
    ReactomeiR-HSA-2132295 MHC class II antigen presentation
    SABIO-RKiP14091

    Protein family/group databases

    MEROPSiA01.010

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cathepsin E (EC:3.4.23.34)
    Cleaved into the following 2 chains:
    Gene namesi
    Name:CTSE
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000196188.10
    HGNCiHGNC:2530 CTSE
    MIMi116890 gene
    neXtProtiNX_P14091

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi60C → A: Abolishes homodimerization. 1 Publication1

    Organism-specific databases

    DisGeNETi1510
    OpenTargetsiENSG00000196188
    PharmGKBiPA27030

    Chemistry databases

    ChEMBLiCHEMBL3092
    GuidetoPHARMACOLOGYi2346

    Polymorphism and mutation databases

    BioMutaiCTSE
    DMDMi46397366

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 191 PublicationAdd BLAST19
    PropeptideiPRO_000002597420 – 53Activation peptide2 PublicationsAdd BLAST34
    ChainiPRO_000002597554 – 396Cathepsin E form IAdd BLAST343
    ChainiPRO_000035466857 – 396Cathepsin E form IIAdd BLAST340

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi60Interchain1 Publication
    Glycosylationi90N-linked (GlcNAc...) asparagine1
    Disulfide bondi109 ↔ 114By similarity
    Disulfide bondi272 ↔ 276By similarity
    Disulfide bondi314 ↔ 351By similarity

    Post-translational modificationi

    Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.3 Publications
    Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.

    Keywords - PTMi

    Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP14091
    PeptideAtlasiP14091
    PRIDEiP14091
    ProteomicsDBi53019
    53020 [P14091-1]
    53021 [P14091-2]

    PTM databases

    iPTMnetiP14091
    PhosphoSitePlusiP14091

    Miscellaneous databases

    PMAP-CutDBiP14091

    Expressioni

    Tissue specificityi

    Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.3 Publications

    Gene expression databases

    BgeeiENSG00000196188 Expressed in 96 organ(s), highest expression level in duodenum
    CleanExiHS_CTSE
    GenevisibleiP14091 HS

    Organism-specific databases

    HPAiCAB032687
    HPA012940

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.1 Publication

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi107890, 8 interactors
    IntActiP14091, 2 interactors
    STRINGi9606.ENSP00000350911

    Chemistry databases

    BindingDBiP14091

    Structurei

    Secondary structure

    1396
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP14091
    SMRiP14091
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14091

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini78 – 396Peptidase A1PROSITE-ProRule annotationAdd BLAST319

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG1339 Eukaryota
    ENOG410XNV7 LUCA
    GeneTreeiENSGT00760000118929
    HOGENOMiHOG000197681
    HOVERGENiHBG000482
    InParanoidiP14091
    KOiK01382
    OMAiKEPGQTF
    OrthoDBiEOG091G0JP7
    PhylomeDBiP14091
    TreeFamiTF314990

    Family and domain databases

    Gene3Di2.40.70.10, 2 hits
    InterProiView protein in InterPro
    IPR001461 Aspartic_peptidase_A1
    IPR001969 Aspartic_peptidase_AS
    IPR012848 Aspartic_peptidase_N
    IPR033145 Cathepsin_E
    IPR033121 PEPTIDASE_A1
    IPR021109 Peptidase_aspartic_dom_sf
    PANTHERiPTHR13683 PTHR13683, 1 hit
    PTHR13683:SF81 PTHR13683:SF81, 1 hit
    PfamiView protein in Pfam
    PF07966 A1_Propeptide, 1 hit
    PF00026 Asp, 1 hit
    PRINTSiPR00792 PEPSIN
    SUPFAMiSSF50630 SSF50630, 1 hit
    PROSITEiView protein in PROSITE
    PS00141 ASP_PROTEASE, 2 hits
    PS51767 PEPTIDASE_A1, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
    Isoform 1 (identifier: P14091-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MKTLLLLLLV LLELGEAQGS LHRVPLRRHP SLKKKLRARS QLSEFWKSHN
    60 70 80 90 100
    LDMIQFTESC SMDQSAKEPL INYLDMEYFG TISIGSPPQN FTVIFDTGSS
    110 120 130 140 150
    NLWVPSVYCT SPACKTHSRF QPSQSSTYSQ PGQSFSIQYG TGSLSGIIGA
    160 170 180 190 200
    DQVSVEGLTV VGQQFGESVT EPGQTFVDAE FDGILGLGYP SLAVGGVTPV
    210 220 230 240 250
    FDNMMAQNLV DLPMFSVYMS SNPEGGAGSE LIFGGYDHSH FSGSLNWVPV
    260 270 280 290 300
    TKQAYWQIAL DNIQVGGTVM FCSEGCQAIV DTGTSLITGP SDKIKQLQNA
    310 320 330 340 350
    IGAAPVDGEY AVECANLNVM PDVTFTINGV PYTLSPTAYT LLDFVDGMQF
    360 370 380 390
    CSSGFQGLDI HPPAGPLWIL GDVFIRQFYS VFDRGNNRVG LAPAVP
    Length:396
    Mass (Da):42,794
    Last modified:March 28, 2018 - v3
    Checksum:i40B643C5FB01521E
    GO
    Isoform 2 (identifier: P14091-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         263-395: IQVGGTVMFC...NNRVGLAPAV → MLWSVPTLTS...PCVCACLSDR

    Show »
    Length:363
    Mass (Da):39,553
    Checksum:i4443819D6393C1FC
    GO
    Isoform 3 (identifier: P14091-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         154-154: S → SAFATQ

    Note: Dubious isoform produced through aberrant splice sites.Curated
    Show »
    Length:401
    Mass (Da):43,312
    Checksum:i46A2BA35266032CB
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_06173182I → V. Corresponds to variant dbSNP:rs57621203Ensembl.1
    Natural variantiVAR_014572324T → I. Corresponds to variant dbSNP:rs6503Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_059425154S → SAFATQ in isoform 3. 1 Publication1
    Alternative sequenceiVSP_059426263 – 395IQVGG…LAPAV → MLWSVPTLTSCRMSPSPLTE SPIPSAQLPTPYWTSWMECS SAAVAFKDLTSTLQLGPSGS WGMSSFDSFTQSLTVGITVW DWPQQSPKEGPCVCACLSDR in isoform 2. 1 PublicationAdd BLAST133

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J05036 mRNA Translation: AAA52130.1
    M84424
    , M84413, M84417, M84418, M84419, M84420, M84421, M84422 Genomic DNA Translation: AAA52300.1
    AJ250716 mRNA Translation: CAB82849.1
    AJ250717 mRNA Translation: CAB82850.1
    AK292057 mRNA Translation: BAF84746.1
    BX571818 Genomic DNA No translation available.
    CH471067 Genomic DNA Translation: EAW91592.1
    CH471067 Genomic DNA Translation: EAW91593.1
    BC042537 mRNA Translation: AAH42537.1
    CCDSiCCDS73012.1 [P14091-2]
    CCDS73013.1 [P14091-1]
    PIRiA42038 A34401
    RefSeqiNP_001304260.1, NM_001317331.1
    NP_001901.1, NM_001910.3 [P14091-1]
    NP_683865.1, NM_148964.2 [P14091-2]
    UniGeneiHs.644082

    Genome annotation databases

    EnsembliENST00000358184; ENSP00000350911; ENSG00000196188 [P14091-1]
    ENST00000360218; ENSP00000353350; ENSG00000196188 [P14091-2]
    GeneIDi1510
    KEGGihsa:1510
    UCSCiuc001hdu.3 human [P14091-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiCATE_HUMAN
    AccessioniPrimary (citable) accession number: P14091
    Secondary accession number(s): Q5TZ01
    , Q5TZ02, Q9NY58, Q9UCE3, Q9UCE4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: March 28, 2018
    Last modified: September 12, 2018
    This is version 197 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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