UniProtKB - P14081 (SELB_ECOLI)
Protein
Selenocysteine-specific elongation factor
Gene
selB
Organism
Escherichia coli (strain K12)
Status
Functioni
Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
Miscellaneous
There are about 1100 copies of SelB per E.coli cell.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 7 – 14 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 112 – 115 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: EcoCyc
- GTPase activity Source: EcoCyc
- GTP binding Source: EcoCyc
- selenocysteine insertion sequence binding Source: EcoCyc
- translation elongation factor activity Source: InterPro
- tRNA binding Source: EcoCyc
GO - Biological processi
- selenocysteine incorporation Source: EcoCyc
- selenocysteine metabolic process Source: EcoCyc
Keywordsi
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10942-MONOMER MetaCyc:EG10942-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Selenocysteine-specific elongation factorAlternative name(s): SelB translation factor |
Gene namesi | Name:selB Synonyms:fdhA Ordered Locus Names:b3590, JW3563 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091474 | 1 – 614 | Selenocysteine-specific elongation factorAdd BLAST | 614 |
Proteomic databases
jPOSTi | P14081 |
PaxDbi | P14081 |
PRIDEi | P14081 |
Interactioni
Binary interactionsi
P14081
With | #Exp. | IntAct |
---|---|---|
serC [P23721] | 3 | EBI-551705,EBI-557952 |
Protein-protein interaction databases
BioGRIDi | 4261875, 9 interactors |
DIPi | DIP-10848N |
IntActi | P14081, 42 interactors |
STRINGi | 511145.b3590 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14081 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14081 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 173 | tr-type GPROSITE-ProRule annotationAdd BLAST | 173 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 7 – 14 | G1PROSITE-ProRule annotation | 8 | |
Regioni | 35 – 39 | G2PROSITE-ProRule annotation | 5 | |
Regioni | 57 – 60 | G3PROSITE-ProRule annotation | 4 | |
Regioni | 112 – 115 | G4PROSITE-ProRule annotation | 4 | |
Regioni | 147 – 149 | G5PROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | COG3276, Bacteria |
HOGENOMi | CLU_023030_2_0_6 |
InParanoidi | P14081 |
PhylomeDBi | P14081 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR015190, Elong_fac_SelB-wing-hlx_typ-2 IPR015191, Elong_fac_SelB-wing-hlx_typ-3 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004535, Transl_elong_SelB IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF09106, SelB-wing_2, 1 hit PF09107, SelB-wing_3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF46785, SSF46785, 3 hits SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00475, selB, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P14081-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIIATAGHVD HGKTTLLQAI TGVNADRLPE EKKRGMTIDL GYAYWPQPDG
60 70 80 90 100
RVPGFIDVPG HEKFLSNMLA GVGGIDHALL VVACDDGVMA QTREHLAILQ
110 120 130 140 150
LTGNPMLTVA LTKADRVDEA RVDEVERQVK EVLREYGFAE AKLFITAATE
160 170 180 190 200
GRGMDALREH LLQLPEREHA SQHSFRLAID RAFTVKGAGL VVTGTALSGE
210 220 230 240 250
VKVGDSLWLT GVNKPMRVRA LHAQNQPTET ANAGQRIALN IAGDAEKEQI
260 270 280 290 300
NRGDWLLADV PPEPFTRVIV ELQTHTPLTQ WQPLHIHHAA SHVTGRVSLL
310 320 330 340 350
EDNLAELVFD TPLWLADNDR LVLRDISARN TLAGARVVML NPPRRGKRKP
360 370 380 390 400
EYLQWLASLA RAQSDADALS VHLERGAVNL ADFAWARQLN GEGMRELLQQ
410 420 430 440 450
PGYIQAGYSL LNAPVAARWQ RKILDTLATY HEQHRDEPGP GRERLRRMAL
460 470 480 490 500
PMEDEALVLL LIEKMRESGD IHSHHGWLHL PDHKAGFSEE QQAIWQKAEP
510 520 530 540 550
LFGDEPWWVR DLAKETGTDE QAMRLTLRQA AQQGIITAIV KDRYYRNDRI
560 570 580 590 600
VEFANMIRDL DQECGSTCAA DFRDRLGVGR KLAIQILEYF DRIGFTRRRG
610
NDHLLRDALL FPEK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16644 Genomic DNA Translation: CAA34637.1 U00039 Genomic DNA Translation: AAB18567.1 Frameshift. U00096 Genomic DNA Translation: AAC76614.1 AP009048 Genomic DNA Translation: BAE77703.1 |
PIRi | JV0050, EFECSB |
RefSeqi | NP_418047.1, NC_000913.3 WP_000582468.1, NZ_SSZK01000041.1 |
Genome annotation databases
EnsemblBacteriai | AAC76614; AAC76614; b3590 BAE77703; BAE77703; BAE77703 |
GeneIDi | 948103 |
KEGGi | ecj:JW3563 eco:b3590 |
PATRICi | fig|1411691.4.peg.3121 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16644 Genomic DNA Translation: CAA34637.1 U00039 Genomic DNA Translation: AAB18567.1 Frameshift. U00096 Genomic DNA Translation: AAC76614.1 AP009048 Genomic DNA Translation: BAE77703.1 |
PIRi | JV0050, EFECSB |
RefSeqi | NP_418047.1, NC_000913.3 WP_000582468.1, NZ_SSZK01000041.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PJP | X-ray | 2.30 | A | 487-607 | [»] | |
5LZB | electron microscopy | 5.30 | z | 1-614 | [»] | |
5LZC | electron microscopy | 4.80 | z | 1-614 | [»] | |
5LZD | electron microscopy | 3.40 | z | 1-614 | [»] | |
SMRi | P14081 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261875, 9 interactors |
DIPi | DIP-10848N |
IntActi | P14081, 42 interactors |
STRINGi | 511145.b3590 |
Proteomic databases
jPOSTi | P14081 |
PaxDbi | P14081 |
PRIDEi | P14081 |
Genome annotation databases
EnsemblBacteriai | AAC76614; AAC76614; b3590 BAE77703; BAE77703; BAE77703 |
GeneIDi | 948103 |
KEGGi | ecj:JW3563 eco:b3590 |
PATRICi | fig|1411691.4.peg.3121 |
Organism-specific databases
EchoBASEi | EB0935 |
Phylogenomic databases
eggNOGi | COG3276, Bacteria |
HOGENOMi | CLU_023030_2_0_6 |
InParanoidi | P14081 |
PhylomeDBi | P14081 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10942-MONOMER MetaCyc:EG10942-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P14081 |
PROi | PR:P14081 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR015190, Elong_fac_SelB-wing-hlx_typ-2 IPR015191, Elong_fac_SelB-wing-hlx_typ-3 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004535, Transl_elong_SelB IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF09106, SelB-wing_2, 1 hit PF09107, SelB-wing_3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF46785, SSF46785, 3 hits SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00475, selB, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SELB_ECOLI | |
Accessioni | P14081Primary (citable) accession number: P14081 Secondary accession number(s): Q2M7Q3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 173 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families