HAP4

Functionⁱ

Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP4 encodes a regulatory subunit of the DNA-bound complex and seems to provide the principal transcriptional activation domains. Does not bind DNA directly, but augments the binding of HAP2 and HAP3.2 Publications

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW
RNA polymerase II activating transcription factor binding
Source: SGD
Inferred from direct assayⁱ
- 11423663

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

carbon catabolite activation of transcription from RNA polymerase II promoter
Source: SGD
Inferred from mutant phenotypeⁱ
- 2676721
regulation of cellular respiration
Source: SGD
Inferred from mutant phenotypeⁱ
- 2676721

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31895-MONOMER

BioCycⁱ

YEAST:G3O-31895-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcriptional activator HAP4
Gene namesⁱ	Name:HAP4 Ordered Locus Names:YKL109W ORF Names:YKL465
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XI

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000001592 HAP4

SGDⁱ

S000001592 HAP4

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	148 – 151	FLKF → SSKS: Greatly diminishes transcriptional activation. 1 Publication	4
Mutagenesisⁱ	390 – 394	IWNYL → SSNSS: Greatly diminishes transcriptional activation. 1 Publication	5
Mutagenesisⁱ	427 – 430	YLFL → SSSS: Greatly diminishes transcriptional activation. 1 Publication	4
Mutagenesisⁱ	456 – 459	FSYL → SSSS: Greatly diminishes transcriptional activation. 1 Publication	4
Mutagenesisⁱ	466 – 467	LM → SS: Abolishes transcriptional activation.	2
Mutagenesisⁱ	466	L → A: Greatly diminishes transcriptional activation. 1 Publication	1
Mutagenesisⁱ	467	M → A: Abolishes transcriptional activation. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000083900	1 – 554	Transcriptional activator HAP4Add BLAST		554

Proteomic databases

PaxDbⁱ	P14064
PRIDEⁱ	P14064

PTM databases

iPTMnetⁱ	P14064

iPTMnetⁱ

P14064

Expressionⁱ

Inductionⁱ

Its expression is glucose-repressible.

Interactionⁱ

Subunit structureⁱ

Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.1 Publication

GO - Molecular functionⁱ

RNA polymerase II activating transcription factor binding
Source: SGD
Inferred from direct assayⁱ
- 11423663

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	34024, 284 interactors
ComplexPortalⁱ	CPX-1830 CCAAT-binding factor complex
Database of interacting proteins More...DIPⁱ	DIP-2360N
IntActⁱ	P14064, 3 interactors
Molecular INTeraction database More...MINTⁱ	P14064
STRINGⁱ	4932.YKL109W

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P14064
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 327	Probably encodes all the information necessary to anchor HAP4 to HAP2/3Add BLAST	327
Regionⁱ	124 – 300	Activation domain 1Add BLAST	177
Regionⁱ	359 – 476	Activation domain 2Add BLAST	118

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	54 – 80	Arg/Lys-rich (basic)Add BLAST	27
Compositional biasⁱ	90 – 113	Asn-richAdd BLAST	24
Compositional biasⁱ	107 – 113	Poly-Asn	7
Compositional biasⁱ	424 – 471	Asp/Glu-rich (acidic)Add BLAST	48
Compositional biasⁱ	519 – 549	Asp/Glu-rich (acidic)Add BLAST	31

Phylogenomic databases

InParanoidⁱ	P14064
OMAⁱ	HIQTIDE
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C22W5

Family and domain databases

InterProⁱ	View protein in InterPro IPR018287 Hap4_TF_heteromerisation
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10297 Hap4_Hap_bind, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P14064-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTAKTFLLQA SASRPRSNHF KNEHNNIPLA PVPIAPNTNH HNNSSLEFEN 
        60         70         80         90        100
DGSKKKKKSS LVVRTSKHWV LPPRPRPGRR SSSHNTLPAN NTNNILNVGP 
       110        120        130        140        150
NSRNSSNNNN NNNIISNRKQ ASKEKRKIPR HIQTIDEKLI NDSNYLAFLK 
       160        170        180        190        200
FDDLENEKFH SSASSISSPS YSSPSFSSYR NRKKSEFMDD ESCTDVETIA 
       210        220        230        240        250
AHNSLLTKNH HIDSSSNVHA PPTKKSKLND FDLLSLSSTS SSATPVPQLT 
       260        270        280        290        300
KDLNMNLNFH KIPHKASFPD SPADFSPADS VSLIRNHSLP TNLQVKDKIE 
       310        320        330        340        350
DLNEIKFFND FEKLEFFNKY AKVNTNNDVN ENNDLWNSYL QSMDDTTGKN 
       360        370        380        390        400
SGNYQQVDND DNMSLLNLPI LEETVSSGQD DKVEPDEEDI WNYLPSSSSQ 
       410        420        430        440        450
QEDSSRALKK NTNSEKANIQ AKNDETYLFL QDQDESADSH HHDELGSEIT 
       460        470        480        490        500
LADNKFSYLP PTLEELMEEQ DCNNGRSFKN FMFSNDTGID GSAGTDDDYT 
       510        520        530        540        550
KVLKSKKIST SKSNANLYDL NDNNNDATAT NELDQSSFID DLDEDVDFLK 

VQVF

Length:554

Mass (Da):62,412

Last modified:June 1, 1994 - v2

Checksum:ⁱCF8A46219F8CA431

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	160	H → R in CAA34707 (PubMed:2676721).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X16727 Genomic DNA Translation: CAA34707.1 X71133 Genomic DNA Translation: CAA50448.1 Z28109 Genomic DNA Translation: CAA81949.1 BK006944 Genomic DNA Translation: DAA09048.1
PIRⁱ	S37936
NCBI Reference Sequences More...RefSeqⁱ	NP_012813.1, NM_001179675.1

Genome annotation databases

EnsemblFungiⁱ	YKL109W; YKL109W; YKL109W
GeneIDⁱ	853751
KEGGⁱ	sce:YKL109W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P14064	Hap4p		YEASC		554	UniRef100_P14064
Transcriptional activator protein of CYC1		YEAS7		554

Protein	Similar proteins		Species	Score	Length	Source
P14064	Hap4p		YEASC		554	UniRef90_P14064
Transcriptional activator protein of CYC1		YEAS7		554
Hap4p		YEASB		553
Transcriptional activator protein of CYC1		YEAS1		553
Hap4p		YEAS8		553
+5

Protein	Similar proteins		Species	Score	Length	Source
P14064	Hap4p		YEASC		554	UniRef50_P14064
Transcriptional activator protein of CYC1		YEAS7		554
Hap4p		YEASB		553
Hap4p		YEAS2		553
Transcriptional activator protein of CYC1		YEAS1		553
+7

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X16727 Genomic DNA Translation: CAA34707.1 X71133 Genomic DNA Translation: CAA50448.1 Z28109 Genomic DNA Translation: CAA81949.1 BK006944 Genomic DNA Translation: DAA09048.1
PIRⁱ	S37936
RefSeqⁱ	NP_012813.1, NM_001179675.1

3D structure databases

ProteinModelPortalⁱ	P14064
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	34024, 284 interactors
ComplexPortalⁱ	CPX-1830 CCAAT-binding factor complex
DIPⁱ	DIP-2360N
IntActⁱ	P14064, 3 interactors
MINTⁱ	P14064
STRINGⁱ	4932.YKL109W

PTM databases

iPTMnetⁱ	P14064

iPTMnetⁱ

P14064

Proteomic databases

PaxDbⁱ	P14064
PRIDEⁱ	P14064

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YKL109W; YKL109W; YKL109W
GeneIDⁱ	853751
KEGGⁱ	sce:YKL109W

Organism-specific databases

SGDⁱ	S000001592 HAP4

Phylogenomic databases

InParanoidⁱ	P14064
OMAⁱ	HIQTIDE
OrthoDBⁱ	EOG092C22W5

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31895-MONOMER

BioCycⁱ

YEAST:G3O-31895-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P14064

PROⁱ

PR:P14064

Family and domain databases

InterProⁱ	View protein in InterPro IPR018287 Hap4_TF_heteromerisation
Pfamⁱ	View protein in Pfam PF10297 Hap4_Hap_bind, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HAP4_YEAST
Accessionⁱ	P14064Primary (citable) accession number: P14064 Secondary accession number(s): D6VXH8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
	Last sequence update:	June 1, 1994
	Last modified:	November 7, 2018
	This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P14064 (HAP4_YEAST)

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Transcriptional activator HAP4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Mitochondrion

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Compositional bias

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ