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UniProtKB - P14064 (HAP4_YEAST)

Entry version 156 (23 Feb 2022)
Sequence version 2 (01 Jun 1994)
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Protein

Transcriptional activator HAP4

Gene

HAP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP4 encodes a regulatory subunit of the DNA-bound complex and seems to provide the principal transcriptional activation domains. Does not bind DNA directly, but augments the binding of HAP2 and HAP3.

2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcriptional activator HAP4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:HAP4
Ordered Locus Names:YKL109W
ORF Names:YKL465
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XI

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000001592, HAP4

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YKL109W

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi148 – 151FLKF → SSKS: Greatly diminishes transcriptional activation. 1 Publication4
Mutagenesisi390 – 394IWNYL → SSNSS: Greatly diminishes transcriptional activation. 1 Publication5
Mutagenesisi427 – 430YLFL → SSSS: Greatly diminishes transcriptional activation. 1 Publication4
Mutagenesisi456 – 459FSYL → SSSS: Greatly diminishes transcriptional activation. 1 Publication4
Mutagenesisi466 – 467LM → SS: Abolishes transcriptional activation. 2
Mutagenesisi466L → A: Greatly diminishes transcriptional activation. 1 Publication1
Mutagenesisi467M → A: Abolishes transcriptional activation. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000839001 – 554Transcriptional activator HAP4Add BLAST554

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P14064

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P14064

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Its expression is glucose-repressible.

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.

1 Publication

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		34024, 288 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-1830, CCAAT-binding factor complex

Database of interacting proteins

More...DIPi		DIP-2360N

Protein interaction database and analysis system

More...IntActi		P14064, 3 interactors

Molecular INTeraction database

More...MINTi		P14064

STRING: functional protein association networks

More...STRINGi		4932.YKL109W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P14064, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 327Probably encodes all the information necessary to anchor HAP4 to HAP2/3Add BLAST327
Regioni1 – 127DisorderedSequence analysisAdd BLAST127
Regioni124 – 300Activation domain 1Add BLAST177
Regioni359 – 476Activation domain 2Add BLAST118
Regioni393 – 422DisorderedSequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1 – 16Polar residuesSequence analysisAdd BLAST16
Compositional biasi79 – 118Polar residuesSequence analysisAdd BLAST40

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502SGDX, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_036426_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P14064

Identification of Orthologs from Complete Genome Data

More...OMAi		HIQTIDE

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR018287, Hap4_TF_heteromerisation

Pfam protein domain database

More...Pfami		View protein in Pfam
PF10297, Hap4_Hap_bind, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P14064-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAKTFLLQA SASRPRSNHF KNEHNNIPLA PVPIAPNTNH HNNSSLEFEN 
        60         70         80         90        100
DGSKKKKKSS LVVRTSKHWV LPPRPRPGRR SSSHNTLPAN NTNNILNVGP 
       110        120        130        140        150
NSRNSSNNNN NNNIISNRKQ ASKEKRKIPR HIQTIDEKLI NDSNYLAFLK 
       160        170        180        190        200
FDDLENEKFH SSASSISSPS YSSPSFSSYR NRKKSEFMDD ESCTDVETIA 
       210        220        230        240        250
AHNSLLTKNH HIDSSSNVHA PPTKKSKLND FDLLSLSSTS SSATPVPQLT 
       260        270        280        290        300
KDLNMNLNFH KIPHKASFPD SPADFSPADS VSLIRNHSLP TNLQVKDKIE 
       310        320        330        340        350
DLNEIKFFND FEKLEFFNKY AKVNTNNDVN ENNDLWNSYL QSMDDTTGKN 
       360        370        380        390        400
SGNYQQVDND DNMSLLNLPI LEETVSSGQD DKVEPDEEDI WNYLPSSSSQ 
       410        420        430        440        450
QEDSSRALKK NTNSEKANIQ AKNDETYLFL QDQDESADSH HHDELGSEIT 
       460        470        480        490        500
LADNKFSYLP PTLEELMEEQ DCNNGRSFKN FMFSNDTGID GSAGTDDDYT 
       510        520        530        540        550
KVLKSKKIST SKSNANLYDL NDNNNDATAT NELDQSSFID DLDEDVDFLK 

VQVF
Length:554
Mass (Da):62,412
Last modified:June 1, 1994 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCF8A46219F8CA431
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti160H → R in CAA34707 (PubMed:2676721).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X16727 Genomic DNA Translation: CAA34707.1
X71133 Genomic DNA Translation: CAA50448.1
Z28109 Genomic DNA Translation: CAA81949.1
BK006944 Genomic DNA Translation: DAA09048.1

Protein sequence database of the Protein Information Resource

More...PIRi		S37936

NCBI Reference Sequences

More...RefSeqi		NP_012813.1, NM_001179675.1

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YKL109W_mRNA; YKL109W; YKL109W

Database of genes from NCBI RefSeq genomes

More...GeneIDi		853751

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YKL109W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16727 Genomic DNA Translation: CAA34707.1
X71133 Genomic DNA Translation: CAA50448.1
Z28109 Genomic DNA Translation: CAA81949.1
BK006944 Genomic DNA Translation: DAA09048.1
PIRiS37936
RefSeqiNP_012813.1, NM_001179675.1

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi34024, 288 interactors
ComplexPortaliCPX-1830, CCAAT-binding factor complex
DIPiDIP-2360N
IntActiP14064, 3 interactors
MINTiP14064
STRINGi4932.YKL109W

PTM databases

iPTMnetiP14064

Proteomic databases

PaxDbiP14064

Genome annotation databases

EnsemblFungiiYKL109W_mRNA; YKL109W; YKL109W
GeneIDi853751
KEGGisce:YKL109W

Organism-specific databases

SGDiS000001592, HAP4
VEuPathDBiFungiDB:YKL109W

Phylogenomic databases

eggNOGiENOG502SGDX, Eukaryota
HOGENOMiCLU_036426_0_0_1
InParanoidiP14064
OMAiHIQTIDE

Miscellaneous databases

Protein Ontology

More...PROi		PR:P14064
RNActiP14064, protein

Family and domain databases

InterProiView protein in InterPro
IPR018287, Hap4_TF_heteromerisation
PfamiView protein in Pfam
PF10297, Hap4_Hap_bind, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHAP4_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P14064
Secondary accession number(s): D6VXH8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 1994
Last modified: February 23, 2022
This is version 156 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
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