UniProtKB - P14064 (HAP4_YEAST)
Transcriptional activator HAP4
HAP4
Functioni
Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP4 encodes a regulatory subunit of the DNA-bound complex and seems to provide the principal transcriptional activation domains. Does not bind DNA directly, but augments the binding of HAP2 and HAP3.
2 PublicationsGO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- RNA polymerase II-specific DNA-binding transcription factor binding Source: SGD
- transcription coactivator activity Source: SGD
GO - Biological processi
- carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
- regulation of cellular respiration Source: SGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator HAP4 |
Gene namesi | Name:HAP4 Ordered Locus Names:YKL109W ORF Names:YKL465 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001592, HAP4 |
VEuPathDBi | FungiDB:YKL109W |
Subcellular locationi
Nucleus
Mitochondrion
- mitochondrial respirasome Source: CACAO
Nucleus
- CCAAT-binding factor complex Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 148 – 151 | FLKF → SSKS: Greatly diminishes transcriptional activation. 1 Publication | 4 | |
Mutagenesisi | 390 – 394 | IWNYL → SSNSS: Greatly diminishes transcriptional activation. 1 Publication | 5 | |
Mutagenesisi | 427 – 430 | YLFL → SSSS: Greatly diminishes transcriptional activation. 1 Publication | 4 | |
Mutagenesisi | 456 – 459 | FSYL → SSSS: Greatly diminishes transcriptional activation. 1 Publication | 4 | |
Mutagenesisi | 466 – 467 | LM → SS: Abolishes transcriptional activation. | 2 | |
Mutagenesisi | 466 | L → A: Greatly diminishes transcriptional activation. 1 Publication | 1 | |
Mutagenesisi | 467 | M → A: Abolishes transcriptional activation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083900 | 1 – 554 | Transcriptional activator HAP4Add BLAST | 554 |
Proteomic databases
PaxDbi | P14064 |
PTM databases
iPTMneti | P14064 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.
1 PublicationGO - Molecular functioni
Protein-protein interaction databases
BioGRIDi | 34024, 288 interactors |
ComplexPortali | CPX-1830, CCAAT-binding factor complex |
DIPi | DIP-2360N |
IntActi | P14064, 3 interactors |
MINTi | P14064 |
STRINGi | 4932.YKL109W |
Miscellaneous databases
RNActi | P14064, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 327 | Probably encodes all the information necessary to anchor HAP4 to HAP2/3Add BLAST | 327 | |
Regioni | 1 – 127 | DisorderedSequence analysisAdd BLAST | 127 | |
Regioni | 124 – 300 | Activation domain 1Add BLAST | 177 | |
Regioni | 359 – 476 | Activation domain 2Add BLAST | 118 | |
Regioni | 393 – 422 | DisorderedSequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 16 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 79 – 118 | Polar residuesSequence analysisAdd BLAST | 40 |
Phylogenomic databases
eggNOGi | ENOG502SGDX, Eukaryota |
HOGENOMi | CLU_036426_0_0_1 |
InParanoidi | P14064 |
OMAi | HIQTIDE |
Family and domain databases
InterProi | View protein in InterPro IPR018287, Hap4_TF_heteromerisation |
Pfami | View protein in Pfam PF10297, Hap4_Hap_bind, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTAKTFLLQA SASRPRSNHF KNEHNNIPLA PVPIAPNTNH HNNSSLEFEN
60 70 80 90 100
DGSKKKKKSS LVVRTSKHWV LPPRPRPGRR SSSHNTLPAN NTNNILNVGP
110 120 130 140 150
NSRNSSNNNN NNNIISNRKQ ASKEKRKIPR HIQTIDEKLI NDSNYLAFLK
160 170 180 190 200
FDDLENEKFH SSASSISSPS YSSPSFSSYR NRKKSEFMDD ESCTDVETIA
210 220 230 240 250
AHNSLLTKNH HIDSSSNVHA PPTKKSKLND FDLLSLSSTS SSATPVPQLT
260 270 280 290 300
KDLNMNLNFH KIPHKASFPD SPADFSPADS VSLIRNHSLP TNLQVKDKIE
310 320 330 340 350
DLNEIKFFND FEKLEFFNKY AKVNTNNDVN ENNDLWNSYL QSMDDTTGKN
360 370 380 390 400
SGNYQQVDND DNMSLLNLPI LEETVSSGQD DKVEPDEEDI WNYLPSSSSQ
410 420 430 440 450
QEDSSRALKK NTNSEKANIQ AKNDETYLFL QDQDESADSH HHDELGSEIT
460 470 480 490 500
LADNKFSYLP PTLEELMEEQ DCNNGRSFKN FMFSNDTGID GSAGTDDDYT
510 520 530 540 550
KVLKSKKIST SKSNANLYDL NDNNNDATAT NELDQSSFID DLDEDVDFLK
VQVF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 160 | H → R in CAA34707 (PubMed:2676721).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16727 Genomic DNA Translation: CAA34707.1 X71133 Genomic DNA Translation: CAA50448.1 Z28109 Genomic DNA Translation: CAA81949.1 BK006944 Genomic DNA Translation: DAA09048.1 |
PIRi | S37936 |
RefSeqi | NP_012813.1, NM_001179675.1 |
Genome annotation databases
EnsemblFungii | YKL109W_mRNA; YKL109W; YKL109W |
GeneIDi | 853751 |
KEGGi | sce:YKL109W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16727 Genomic DNA Translation: CAA34707.1 X71133 Genomic DNA Translation: CAA50448.1 Z28109 Genomic DNA Translation: CAA81949.1 BK006944 Genomic DNA Translation: DAA09048.1 |
PIRi | S37936 |
RefSeqi | NP_012813.1, NM_001179675.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 34024, 288 interactors |
ComplexPortali | CPX-1830, CCAAT-binding factor complex |
DIPi | DIP-2360N |
IntActi | P14064, 3 interactors |
MINTi | P14064 |
STRINGi | 4932.YKL109W |
PTM databases
iPTMneti | P14064 |
Proteomic databases
PaxDbi | P14064 |
Genome annotation databases
EnsemblFungii | YKL109W_mRNA; YKL109W; YKL109W |
GeneIDi | 853751 |
KEGGi | sce:YKL109W |
Organism-specific databases
SGDi | S000001592, HAP4 |
VEuPathDBi | FungiDB:YKL109W |
Phylogenomic databases
eggNOGi | ENOG502SGDX, Eukaryota |
HOGENOMi | CLU_036426_0_0_1 |
InParanoidi | P14064 |
OMAi | HIQTIDE |
Miscellaneous databases
PROi | PR:P14064 |
RNActi | P14064, protein |
Family and domain databases
InterProi | View protein in InterPro IPR018287, Hap4_TF_heteromerisation |
Pfami | View protein in Pfam PF10297, Hap4_Hap_bind, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAP4_YEAST | |
Accessioni | P14064Primary (citable) accession number: P14064 Secondary accession number(s): D6VXH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | June 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names