UniProtKB - P14061 (DHB1_HUMAN)
Protein
17-beta-hydroxysteroid dehydrogenase type 1
Gene
HSD17B1
Organism
Homo sapiens (Human)
Status
Functioni
Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2) (PubMed:8994190). Also has 20-alpha-HSD activity. Uses preferentially NADH.1 Publication
Catalytic activityi
- EC:1.1.1.621 Publication
- EC:1.1.1.62By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:1.1.1.51By similarityThis reaction proceeds in the backwardBy similarity direction.
Kineticsi
- KM=2.11 µM for 17beta-estradiol1 Publication
- KM=0.9 µM for estrone1 Publication
: estrogen biosynthesis Pathwayi
This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 66 | NADP1 Publication | 1 | |
Binding sitei | 143 | Substrate1 Publication | 1 | |
Active sitei | 156 | Proton acceptor | 1 | |
Binding sitei | 160 | NADP1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 38 | NADP1 PublicationAdd BLAST | 29 |
GO - Molecular functioni
- 17-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: UniProtKB
- catalytic activity Source: ProtInc
- estradiol 17-beta-dehydrogenase activity Source: UniProtKB
- estradiol binding Source: CAFA
- NADP+ binding Source: CAFA
- NADP binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- steroid binding Source: CAFA
- testosterone dehydrogenase [NAD(P)] activity Source: UniProtKB
GO - Biological processi
- estrogen biosynthetic process Source: UniProtKB
- estrogen metabolic process Source: ProtInc
- steroid biosynthetic process Source: ProtInc
- testosterone biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.62, 2681 |
PathwayCommonsi | P14061 |
Reactomei | R-HSA-193144, Estrogen biosynthesis R-HSA-2453902, The canonical retinoid cycle in rods (twilight vision) |
SABIO-RKi | P14061 |
UniPathwayi | UPA00769 |
Chemistry databases
SwissLipidsi | SLP:000001218 |
Names & Taxonomyi
Protein namesi | Recommended name: 17-beta-hydroxysteroid dehydrogenase type 1Curated (EC:1.1.1.51By similarity)Short name: 17-beta-HSD 1 Alternative name(s): 20 alpha-hydroxysteroid dehydrogenase Short name: 20-alpha-HSD E2DH Estradiol 17-beta-dehydrogenase 1Curated (EC:1.1.1.621 Publication) Placental 17-beta-hydroxysteroid dehydrogenase Short chain dehydrogenase/reductase family 28C member 1 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000108786.10 |
HGNCi | HGNC:5210, HSD17B1 |
MIMi | 109684, gene |
neXtProti | NX_P14061 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 112 – 114 | LDV → F: Loss of 17-beta-hydroxysteroid dehydrogenase activity. 1 Publication | 3 | |
Mutagenesisi | 135 | S → A: Abolishes phosphorylation. No effect on 17-beta-hydroxysteroid dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 143 | S → A: Loss of 17-beta-hydroxysteroid dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 150 | L → V: Alters substrate specificity. 1 Publication | 1 | |
Mutagenesisi | 156 | Y → A: Loss of 17-beta-hydroxysteroid dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 160 | K → A: Loss of 17-beta-hydroxysteroid dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 171 | A → AEF: Loss of 17-beta-hydroxysteroid dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 222 | H → A: No effect on conversion of estrone to 17beta-estradiol. 1 Publication | 1 | |
Mutagenesisi | 283 | E → A: No effect on conversion of estrone to 17beta-estradiol. 1 Publication | 1 | |
Mutagenesisi | 283 | E → Q: No effect on conversion of estrone to 17beta-estradiol. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 3292 |
OpenTargetsi | ENSG00000108786 |
PharmGKBi | PA29478 |
Miscellaneous databases
Pharosi | P14061, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3181 |
DrugBanki | DB01561, Androstanedione DB01536, Androstenedione DB02323, EM-1745 DB02187, Equilin DB00157, NADH DB03461, Nicotinamide adenine dinucleotide phosphate DB01708, Prasterone DB02901, Stanolone DB13951, Stanolone acetate |
DrugCentrali | P14061 |
Polymorphism and mutation databases
BioMutai | HSD17B1 |
DMDMi | 313104233 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000054567 | 2 – 328 | 17-beta-hydroxysteroid dehydrogenase type 1Add BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 135 | Phosphoserine; by PKA1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P14061 |
MassIVEi | P14061 |
PaxDbi | P14061 |
PeptideAtlasi | P14061 |
PRIDEi | P14061 |
ProteomicsDBi | 53018 |
PTM databases
SwissPalmi | P14061 |
Expressioni
Gene expression databases
Bgeei | ENSG00000108786, Expressed in chorionic villus and 139 other tissues |
ExpressionAtlasi | P14061, baseline and differential |
Genevisiblei | P14061, HS |
Organism-specific databases
HPAi | ENSG00000108786, Tissue enriched (placenta) |
Interactioni
Subunit structurei
Homodimer. Exists predominantly as an homodimer but also exits as monomer.
2 PublicationsBinary interactionsi
P14061
With | #Exp. | IntAct |
---|---|---|
ALOX5 [P09917] | 3 | EBI-12867244,EBI-79934 |
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109525, 16 interactors |
IntActi | P14061, 2 interactors |
STRINGi | 9606.ENSP00000466799 |
Chemistry databases
BindingDBi | P14061 |
Miscellaneous databases
RNActi | P14061, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P14061 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P14061 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG1205, Eukaryota |
GeneTreei | ENSGT00940000160415 |
HOGENOMi | CLU_010194_2_9_1 |
InParanoidi | P14061 |
PhylomeDBi | P14061 |
Family and domain databases
DisProti | DP00023 |
InterProi | View protein in InterPro IPR011348, 17beta_DH IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PIRSFi | PIRSF000095, 17beta-HSD, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P14061-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARTVVLITG CSSGIGLHLA VRLASDPSQS FKVYATLRDL KTQGRLWEAA
60 70 80 90 100
RALACPPGSL ETLQLDVRDS KSVAAARERV TEGRVDVLVC NAGLGLLGPL
110 120 130 140 150
EALGEDAVAS VLDVNVVGTV RMLQAFLPDM KRRGSGRVLV TGSVGGLMGL
160 170 180 190 200
PFNDVYCASK FALEGLCESL AVLLLPFGVH LSLIECGPVH TAFMEKVLGS
210 220 230 240 250
PEEVLDRTDI HTFHRFYQYL AHSKQVFREA AQNPEEVAEV FLTALRAPKP
260 270 280 290 300
TLRYFTTERF LPLLRMRLDD PSGSNYVTAM HREVFGDVPA KAEAGAEAGG
310 320
GAGPGAEDEA GRGAVGDPEL GDPPAAPQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MQS7 | A0A0A0MQS7_HUMAN | Estradiol 17-beta-dehydrogenase | HSD17B1 | 329 | Annotation score: | ||
B4DU11 | B4DU11_HUMAN | Estradiol 17-beta-dehydrogenase 1 | HSD17B1 | 149 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | R → E AA sequence (PubMed:5045524).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_006951 | 238 | A → V1 PublicationCorresponds to variant dbSNP:rs147402365Ensembl. | 1 | |
Natural variantiVAR_006952 | 313 | G → S10 PublicationsCorresponds to variant dbSNP:rs605059Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13440 mRNA Translation: CAA31792.1 M36263 mRNA Translation: AAA35600.1 M27138 Genomic DNA Translation: AAB16941.1 M84472 Genomic DNA Translation: AAB16942.1 U34879 Genomic DNA Translation: AAD05019.1 AK127832 mRNA Translation: BAG54583.1 AC067852 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60836.1 BC104752 mRNA Translation: AAI04753.1 BC111935 mRNA Translation: AAI11936.1 |
CCDSi | CCDS11428.1 |
PIRi | A36081, DEHUE7 |
RefSeqi | NP_000404.2, NM_000413.3 |
Genome annotation databases
Ensembli | ENST00000585807; ENSP00000466799; ENSG00000108786 |
GeneIDi | 3292 |
KEGGi | hsa:3292 |
UCSCi | uc002hzw.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
SHMPD The Singapore human mutation and polymorphism database |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13440 mRNA Translation: CAA31792.1 M36263 mRNA Translation: AAA35600.1 M27138 Genomic DNA Translation: AAB16941.1 M84472 Genomic DNA Translation: AAB16942.1 U34879 Genomic DNA Translation: AAD05019.1 AK127832 mRNA Translation: BAG54583.1 AC067852 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60836.1 BC104752 mRNA Translation: AAI04753.1 BC111935 mRNA Translation: AAI11936.1 |
CCDSi | CCDS11428.1 |
PIRi | A36081, DEHUE7 |
RefSeqi | NP_000404.2, NM_000413.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A27 | X-ray | 1.90 | A | 2-290 | [»] | |
1BHS | X-ray | 2.20 | A | 2-328 | [»] | |
1DHT | X-ray | 2.24 | A | 2-328 | [»] | |
1EQU | X-ray | 3.00 | A/B | 2-328 | [»] | |
1FDS | X-ray | 1.70 | A | 2-328 | [»] | |
1FDT | X-ray | 2.20 | A | 2-328 | [»] | |
1FDU | X-ray | 2.70 | A/B/C/D | 2-328 | [»] | |
1FDV | X-ray | 3.10 | A/B/C/D | 2-328 | [»] | |
1FDW | X-ray | 2.70 | A | 2-328 | [»] | |
1I5R | X-ray | 1.60 | A | 2-328 | [»] | |
1IOL | X-ray | 2.30 | A | 2-328 | [»] | |
1JTV | X-ray | 1.54 | A | 2-328 | [»] | |
1QYV | X-ray | 1.81 | A | 2-328 | [»] | |
1QYW | X-ray | 1.63 | A | 2-328 | [»] | |
1QYX | X-ray | 1.89 | A | 2-328 | [»] | |
3DEY | X-ray | 1.70 | X | 2-328 | [»] | |
3DHE | X-ray | 2.30 | A | 2-328 | [»] | |
3HB4 | X-ray | 2.21 | X | 2-328 | [»] | |
3HB5 | X-ray | 2.00 | X | 2-328 | [»] | |
3KLM | X-ray | 1.70 | X | 2-328 | [»] | |
3KLP | X-ray | 2.50 | X | 2-328 | [»] | |
3KM0 | X-ray | 2.30 | A/B | 2-328 | [»] | |
6CGC | X-ray | 2.10 | A/B | 1-328 | [»] | |
6CGE | X-ray | 2.20 | A/B | 1-328 | [»] | |
6DTP | X-ray | 2.00 | A/B | 1-328 | [»] | |
6MNC | X-ray | 2.40 | A/B | 1-328 | [»] | |
6MNE | X-ray | 1.86 | A/B | 1-328 | [»] | |
SMRi | P14061 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109525, 16 interactors |
IntActi | P14061, 2 interactors |
STRINGi | 9606.ENSP00000466799 |
Chemistry databases
BindingDBi | P14061 |
ChEMBLi | CHEMBL3181 |
DrugBanki | DB01561, Androstanedione DB01536, Androstenedione DB02323, EM-1745 DB02187, Equilin DB00157, NADH DB03461, Nicotinamide adenine dinucleotide phosphate DB01708, Prasterone DB02901, Stanolone DB13951, Stanolone acetate |
DrugCentrali | P14061 |
SwissLipidsi | SLP:000001218 |
PTM databases
SwissPalmi | P14061 |
Polymorphism and mutation databases
BioMutai | HSD17B1 |
DMDMi | 313104233 |
Proteomic databases
jPOSTi | P14061 |
MassIVEi | P14061 |
PaxDbi | P14061 |
PeptideAtlasi | P14061 |
PRIDEi | P14061 |
ProteomicsDBi | 53018 |
Protocols and materials databases
Antibodypediai | 16966, 284 antibodies |
Genome annotation databases
Ensembli | ENST00000585807; ENSP00000466799; ENSG00000108786 |
GeneIDi | 3292 |
KEGGi | hsa:3292 |
UCSCi | uc002hzw.4, human |
Organism-specific databases
CTDi | 3292 |
DisGeNETi | 3292 |
EuPathDBi | HostDB:ENSG00000108786.10 |
GeneCardsi | HSD17B1 |
HGNCi | HGNC:5210, HSD17B1 |
HPAi | ENSG00000108786, Tissue enriched (placenta) |
MIMi | 109684, gene |
neXtProti | NX_P14061 |
OpenTargetsi | ENSG00000108786 |
PharmGKBi | PA29478 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1205, Eukaryota |
GeneTreei | ENSGT00940000160415 |
HOGENOMi | CLU_010194_2_9_1 |
InParanoidi | P14061 |
PhylomeDBi | P14061 |
Enzyme and pathway databases
UniPathwayi | UPA00769 |
BRENDAi | 1.1.1.62, 2681 |
PathwayCommonsi | P14061 |
Reactomei | R-HSA-193144, Estrogen biosynthesis R-HSA-2453902, The canonical retinoid cycle in rods (twilight vision) |
SABIO-RKi | P14061 |
Miscellaneous databases
BioGRID-ORCSi | 3292, 3 hits in 844 CRISPR screens |
ChiTaRSi | HSD17B1, human |
EvolutionaryTracei | P14061 |
GeneWikii | HSD17B1 |
GenomeRNAii | 3292 |
Pharosi | P14061, Tchem |
PROi | PR:P14061 |
RNActi | P14061, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000108786, Expressed in chorionic villus and 139 other tissues |
ExpressionAtlasi | P14061, baseline and differential |
Genevisiblei | P14061, HS |
Family and domain databases
DisProti | DP00023 |
InterProi | View protein in InterPro IPR011348, 17beta_DH IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PIRSFi | PIRSF000095, 17beta-HSD, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHB1_HUMAN | |
Accessioni | P14061Primary (citable) accession number: P14061 Secondary accession number(s): B3KXS1, Q2M2L8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | November 30, 2010 | |
Last modified: | December 2, 2020 | |
This is version 214 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations