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Protein

General transcription factor IIF subunit 2

Gene

GTF2F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIF subunit 2 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase GTF2F2
General transcription factor IIF 30 kDa subunit
Transcription initiation factor IIF subunit beta
Short name:
TFIIF-beta
Transcription initiation factor RAP30
Gene namesi
Name:GTF2F2
Synonyms:RAP30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000188342.11
HGNCiHGNC:4653 GTF2F2
MIMi189969 gene
neXtProtiNX_P13984

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2963
OpenTargetsiENSG00000188342
PharmGKBiPA29039

Polymorphism and mutation databases

BioMutaiGTF2F2
DMDMi464519

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002112352 – 249General transcription factor IIF subunit 2Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei22N6-acetyllysineCombined sources1
Modified residuei33N6-acetyllysineCombined sources1
Modified residuei137N6-acetyllysineCombined sources1
Modified residuei142PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP13984
MaxQBiP13984
PaxDbiP13984
PeptideAtlasiP13984
PRIDEiP13984
ProteomicsDBi53012

PTM databases

iPTMnetiP13984
PhosphoSitePlusiP13984

Expressioni

Gene expression databases

BgeeiENSG00000188342 Expressed in 222 organ(s), highest expression level in skin of abdomen
CleanExiHS_GTF2F2
ExpressionAtlasiP13984 baseline and differential
GenevisibleiP13984 HS

Organism-specific databases

HPAiHPA006912

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1. Interacts with GTF2B (via N-terminus); this interaction is inhibited in presence of GTF2F1 (PubMed:8504927, PubMed:8662660).By similarity4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi109218, 51 interactors
ComplexPortaliCPX-79 Transcription factor TFIIF complex
CORUMiP13984
DIPiDIP-41680N
IntActiP13984, 43 interactors
MINTiP13984
STRINGi9606.ENSP00000340823

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13984
SMRiP13984
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13984

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIF beta subunit family.Curated

Phylogenomic databases

eggNOGiKOG2905 Eukaryota
COG5090 LUCA
GeneTreeiENSGT00390000016051
HOGENOMiHOG000294179
HOVERGENiHBG001606
InParanoidiP13984
KOiK03139
OMAiNMWELKK
OrthoDBiEOG091G0IN2
PhylomeDBiP13984
TreeFamiTF314290

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003196 TFIIF_beta
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10445 PTHR10445, 1 hit
PfamiView protein in Pfam
PF02270 TFIIF_beta, 1 hit
PIRSFiPIRSF015849 TFIIF-beta, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13984-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG
60 70 80 90 100
RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS
110 120 130 140 150
SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVR LSQQLDKVVT
160 170 180 190 200
TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK
210 220 230 240
DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD
Length:249
Mass (Da):28,380
Last modified:February 1, 1994 - v2
Checksum:i05A1A9F8D31B749C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16901 mRNA Translation: CAA34775.1
X59745 mRNA Translation: CAA42419.1
AK291545 mRNA Translation: BAF84234.1
BT019525 mRNA Translation: AAV38332.1
AL138963 Genomic DNA No translation available.
AL138693 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08728.1
BC001771 mRNA Translation: AAH01771.1
CCDSiCCDS9395.1
PIRiS06141
S18677
RefSeqiNP_004119.1, NM_004128.2
UniGeneiHs.654582

Genome annotation databases

EnsembliENST00000340473; ENSP00000340823; ENSG00000188342
GeneIDi2963
KEGGihsa:2963
UCSCiuc001uzw.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16901 mRNA Translation: CAA34775.1
X59745 mRNA Translation: CAA42419.1
AK291545 mRNA Translation: BAF84234.1
BT019525 mRNA Translation: AAV38332.1
AL138963 Genomic DNA No translation available.
AL138693 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08728.1
BC001771 mRNA Translation: AAH01771.1
CCDSiCCDS9395.1
PIRiS06141
S18677
RefSeqiNP_004119.1, NM_004128.2
UniGeneiHs.654582

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BBYNMR-A175-243[»]
1F3UX-ray1.70A/C/E/G2-119[»]
2BBYNMR-A175-243[»]
5IY6electron microscopy7.20T1-249[»]
5IY7electron microscopy8.60T1-249[»]
5IY8electron microscopy7.90T1-249[»]
5IY9electron microscopy6.30T1-249[»]
5IYAelectron microscopy5.40T1-249[»]
5IYBelectron microscopy3.90T1-249[»]
5IYCelectron microscopy3.90T1-249[»]
5IYDelectron microscopy3.90T1-249[»]
ProteinModelPortaliP13984
SMRiP13984
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109218, 51 interactors
ComplexPortaliCPX-79 Transcription factor TFIIF complex
CORUMiP13984
DIPiDIP-41680N
IntActiP13984, 43 interactors
MINTiP13984
STRINGi9606.ENSP00000340823

PTM databases

iPTMnetiP13984
PhosphoSitePlusiP13984

Polymorphism and mutation databases

BioMutaiGTF2F2
DMDMi464519

Proteomic databases

EPDiP13984
MaxQBiP13984
PaxDbiP13984
PeptideAtlasiP13984
PRIDEiP13984
ProteomicsDBi53012

Protocols and materials databases

DNASUi2963
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340473; ENSP00000340823; ENSG00000188342
GeneIDi2963
KEGGihsa:2963
UCSCiuc001uzw.4 human

Organism-specific databases

CTDi2963
DisGeNETi2963
EuPathDBiHostDB:ENSG00000188342.11
GeneCardsiGTF2F2
HGNCiHGNC:4653 GTF2F2
HPAiHPA006912
MIMi189969 gene
neXtProtiNX_P13984
OpenTargetsiENSG00000188342
PharmGKBiPA29039
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2905 Eukaryota
COG5090 LUCA
GeneTreeiENSGT00390000016051
HOGENOMiHOG000294179
HOVERGENiHBG001606
InParanoidiP13984
KOiK03139
OMAiNMWELKK
OrthoDBiEOG091G0IN2
PhylomeDBiP13984
TreeFamiTF314290

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiGTF2F2 human
EvolutionaryTraceiP13984
GeneWikiiGTF2F2
GenomeRNAii2963
PROiPR:P13984
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188342 Expressed in 222 organ(s), highest expression level in skin of abdomen
CleanExiHS_GTF2F2
ExpressionAtlasiP13984 baseline and differential
GenevisibleiP13984 HS

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003196 TFIIF_beta
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10445 PTHR10445, 1 hit
PfamiView protein in Pfam
PF02270 TFIIF_beta, 1 hit
PIRSFiPIRSF015849 TFIIF-beta, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiT2FB_HUMAN
AccessioniPrimary (citable) accession number: P13984
Secondary accession number(s): A6NNS5, Q5W0H3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1994
Last modified: November 7, 2018
This is version 191 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
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