UniProtKB - P13861 (KAP2_HUMAN)
cAMP-dependent protein kinase type II-alpha regulatory subunit
PRKAR2A
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 208 | cAMP 1 | 1 | |
Binding sitei | 217 | cAMP 1 | 1 | |
Binding sitei | 338 | cAMP 2 | 1 | |
Binding sitei | 347 | cAMP 2 | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 139 – 260 | cAMP 1Add BLAST | 122 | |
Nucleotide bindingi | 261 – 404 | cAMP 2Add BLAST | 144 |
GO - Molecular functioni
- cAMP binding Source: UniProtKB-KW
- cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
- cAMP-dependent protein kinase regulator activity Source: BHF-UCL
- protein domain specific binding Source: UniProtKB
- protein kinase A catalytic subunit binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- activation of protein kinase A activity Source: Reactome
- blood coagulation Source: Reactome
- cellular response to glucagon stimulus Source: Reactome
- intracellular signal transduction Source: ProtInc
- negative regulation of cAMP-dependent protein kinase activity Source: BHF-UCL
- regulation of cAMP-mediated signaling Source: GO_Central
- regulation of protein kinase A signaling Source: GO_Central
- renal water homeostasis Source: Reactome
Keywordsi
Ligand | cAMP, cAMP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P13861 |
Reactomei | R-HSA-163615, PKA activation R-HSA-164378, PKA activation in glucagon signalling R-HSA-180024, DARPP-32 events R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-HSA-5610787, Hedgehog 'off' state R-HSA-9010642, ROBO receptors bind AKAP5 R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-983231, Factors involved in megakaryocyte development and platelet production |
SignaLinki | P13861 |
SIGNORi | P13861 |
Names & Taxonomyi
Protein namesi | Recommended name: cAMP-dependent protein kinase type II-alpha regulatory subunit |
Gene namesi | Name:PRKAR2A Synonyms:PKR2, PRKAR2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000114302.15 |
HGNCi | HGNC:9391, PRKAR2A |
MIMi | 176910, gene |
neXtProti | NX_P13861 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: Colocalizes with PJA2 in the cytoplasm and the cell membrane.
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
- plasma membrane raft Source: UniProtKB
Other locations
- cAMP-dependent protein kinase complex Source: UniProtKB
- ciliary base Source: Reactome
- cytoplasm Source: UniProtKB
- focal adhesion Source: UniProtKB
- membrane Source: UniProtKB
- nucleotide-activated protein kinase complex Source: BHF-UCL
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 5576 |
OpenTargetsi | ENSG00000114302 |
PharmGKBi | PA33757 |
Miscellaneous databases
Pharosi | P13861, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2221 |
DrugBanki | DB05798, GEM-231 |
GuidetoPHARMACOLOGYi | 1474 |
Polymorphism and mutation databases
BioMutai | PRKAR2A |
DMDMi | 125198 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000205385 | 2 – 404 | cAMP-dependent protein kinase type II-alpha regulatory subunitAdd BLAST | 403 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 48 | PhosphoserineCombined sources | 1 | |
Modified residuei | 54 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 78 | PhosphoserineCombined sources | 1 | |
Modified residuei | 80 | PhosphoserineCombined sources | 1 | |
Modified residuei | 99 | Phosphoserine; by PKACombined sources | 1 | |
Modified residuei | 215 | Phosphothreonine; by PDPK1By similarity | 1 | |
Modified residuei | 350 | PhosphoserineCombined sources | 1 | |
Modified residuei | 395 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P13861 |
jPOSTi | P13861 |
MassIVEi | P13861 |
MaxQBi | P13861 |
PaxDbi | P13861 |
PeptideAtlasi | P13861 |
PRIDEi | P13861 |
ProteomicsDBi | 52996 [P13861-1] 79069 |
2D gel databases
OGPi | P13861 |
PTM databases
iPTMneti | P13861 |
PhosphoSitePlusi | P13861 |
SwissPalmi | P13861 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000114302, Expressed in buccal mucosa cell and 255 other tissues |
ExpressionAtlasi | P13861, baseline and differential |
Genevisiblei | P13861, HS |
Organism-specific databases
HPAi | ENSG00000114302, Tissue enhanced (testis) |
Interactioni
Subunit structurei
The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
Interacts with AKAP4 and CBFA2T3.
Interacts with the phosphorylated form of PJA2.
Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity).
Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (PubMed:27484798).
Interacts with ADCY8; inhibits adenylate cyclase activity through PKA phosphorylation (PubMed:22976297).
By similarity2 PublicationsBinary interactionsi
Hide detailsP13861
Isoform 2 [P13861-2]
With | #Exp. | IntAct |
---|---|---|
AKAP14 [Q86UN6] | 3 | EBI-11752137,EBI-2119626 |
AKAP7 - isoform Alpha [O43687-2] | 5 | EBI-11752137,EBI-10185182 |
LRIF1 [Q5T3J3] | 3 | EBI-11752137,EBI-473196 |
GO - Molecular functioni
- protein domain specific binding Source: UniProtKB
- protein kinase A catalytic subunit binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111562, 92 interactors |
CORUMi | P13861 |
DIPi | DIP-552N |
IntActi | P13861, 86 interactors |
MINTi | P13861 |
STRINGi | 9606.ENSP00000265563 |
Miscellaneous databases
RNActi | P13861, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P13861 |
SMRi | P13861 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13861 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 138 | Dimerization and phosphorylationAdd BLAST | 137 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1113, Eukaryota |
GeneTreei | ENSGT00940000154836 |
HOGENOMi | CLU_018310_2_0_1 |
InParanoidi | P13861 |
OMAi | IARCHRG |
PhylomeDBi | P13861 |
TreeFami | TF314920 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits |
DisProti | DP02333 |
Gene3Di | 2.60.120.10, 2 hits |
IDEALi | IID00411 |
InterProi | View protein in InterPro IPR012198, cAMP_dep_PK_reg_su IPR003117, cAMP_dep_PK_reg_su_I/II_a/b IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF02197, RIIa, 1 hit |
PIRSFi | PIRSF000548, PK_regulatory, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00394, RIIa, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits |
PROSITEi | View protein in PROSITE PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSHIQIPPGL TELLQGYTVE VLRQQPPDLV EFAVEYFTRL REARAPASVL
60 70 80 90 100
PAATPRQSLG HPPPEPGPDR VADAKGDSES EEDEDLEVPV PSRFNRRVSV
110 120 130 140 150
CAETYNPDEE EEDTDPRVIH PKTDEQRCRL QEACKDILLF KNLDQEQLSQ
160 170 180 190 200
VLDAMFERIV KADEHVIDQG DDGDNFYVIE RGTYDILVTK DNQTRSVGQY
210 220 230 240 250
DNRGSFGELA LMYNTPRAAT IVATSEGSLW GLDRVTFRRI IVKNNAKKRK
260 270 280 290 300
MFESFIESVP LLKSLEVSER MKIVDVIGEK IYKDGERIIT QGEKADSFYI
310 320 330 340 350
IESGEVSILI RSRTKSNKDG GNQEVEIARC HKGQYFGELA LVTNKPRAAS
360 370 380 390 400
AYAVGDVKCL VMDVQAFERL LGPCMDIMKR NISHYEEQLV KMFGSSVDLG
NLGQ
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J830 | C9J830_HUMAN | cAMP-dependent protein kinase type ... | PRKAR2A | 114 | Annotation score: | ||
H7C1L0 | H7C1L0_HUMAN | cAMP-dependent protein kinase type ... | PRKAR2A | 200 | Annotation score: | ||
H7C330 | H7C330_HUMAN | cAMP-dependent protein kinase type ... | PRKAR2A | 92 | Annotation score: | ||
H7C4A9 | H7C4A9_HUMAN | cAMP-dependent protein kinase type ... | PRKAR2A | 99 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056821 | 292 – 313 | Missing in isoform 2. 2 PublicationsAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14968 mRNA Translation: CAA33094.1 BT007225 mRNA Translation: AAP35889.1 AC141002 Genomic DNA No translation available. AC144546 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64926.1 CH471055 Genomic DNA Translation: EAW64927.1 BC002763 mRNA Translation: AAH02763.1 X99455 Genomic DNA Translation: CAA67817.1 |
CCDSi | CCDS2778.1 [P13861-1] CCDS82771.1 [P13861-2] |
PIRi | S03885, OKHU2R |
RefSeqi | NP_001308911.1, NM_001321982.1 [P13861-1] NP_001308912.1, NM_001321983.1 [P13861-2] NP_004148.1, NM_004157.3 [P13861-1] XP_011532244.1, XM_011533942.2 [P13861-1] XP_016862304.1, XM_017006815.1 |
Genome annotation databases
Ensembli | ENST00000265563; ENSP00000265563; ENSG00000114302 [P13861-1] ENST00000296446; ENSP00000296446; ENSG00000114302 [P13861-2] ENST00000454963; ENSP00000394041; ENSG00000114302 [P13861-1] |
GeneIDi | 5576 |
KEGGi | hsa:5576 |
UCSCi | uc003cux.2, human [P13861-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14968 mRNA Translation: CAA33094.1 BT007225 mRNA Translation: AAP35889.1 AC141002 Genomic DNA No translation available. AC144546 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64926.1 CH471055 Genomic DNA Translation: EAW64927.1 BC002763 mRNA Translation: AAH02763.1 X99455 Genomic DNA Translation: CAA67817.1 |
CCDSi | CCDS2778.1 [P13861-1] CCDS82771.1 [P13861-2] |
PIRi | S03885, OKHU2R |
RefSeqi | NP_001308911.1, NM_001321982.1 [P13861-1] NP_001308912.1, NM_001321983.1 [P13861-2] NP_004148.1, NM_004157.3 [P13861-1] XP_011532244.1, XM_011533942.2 [P13861-1] XP_016862304.1, XM_017006815.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IZX | X-ray | 1.30 | A/B | 4-44 | [»] | |
2KYG | NMR | - | A/B | 1-45 | [»] | |
4ZP3 | X-ray | 2.63 | A/B/C/D/E/F/G/H/I/J/K/L | 2-44 | [»] | |
5H78 | X-ray | 2.00 | A/B | 5-49 | [»] | |
5XBY | X-ray | 3.25 | A/B/C/D | 5-44 | [»] | |
BMRBi | P13861 | |||||
SMRi | P13861 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111562, 92 interactors |
CORUMi | P13861 |
DIPi | DIP-552N |
IntActi | P13861, 86 interactors |
MINTi | P13861 |
STRINGi | 9606.ENSP00000265563 |
Chemistry databases
ChEMBLi | CHEMBL2221 |
DrugBanki | DB05798, GEM-231 |
GuidetoPHARMACOLOGYi | 1474 |
PTM databases
iPTMneti | P13861 |
PhosphoSitePlusi | P13861 |
SwissPalmi | P13861 |
Polymorphism and mutation databases
BioMutai | PRKAR2A |
DMDMi | 125198 |
2D gel databases
OGPi | P13861 |
Proteomic databases
EPDi | P13861 |
jPOSTi | P13861 |
MassIVEi | P13861 |
MaxQBi | P13861 |
PaxDbi | P13861 |
PeptideAtlasi | P13861 |
PRIDEi | P13861 |
ProteomicsDBi | 52996 [P13861-1] 79069 |
Protocols and materials databases
Antibodypediai | 3555, 439 antibodies |
DNASUi | 5576 |
Genome annotation databases
Ensembli | ENST00000265563; ENSP00000265563; ENSG00000114302 [P13861-1] ENST00000296446; ENSP00000296446; ENSG00000114302 [P13861-2] ENST00000454963; ENSP00000394041; ENSG00000114302 [P13861-1] |
GeneIDi | 5576 |
KEGGi | hsa:5576 |
UCSCi | uc003cux.2, human [P13861-1] |
Organism-specific databases
CTDi | 5576 |
DisGeNETi | 5576 |
EuPathDBi | HostDB:ENSG00000114302.15 |
GeneCardsi | PRKAR2A |
HGNCi | HGNC:9391, PRKAR2A |
HPAi | ENSG00000114302, Tissue enhanced (testis) |
MIMi | 176910, gene |
neXtProti | NX_P13861 |
OpenTargetsi | ENSG00000114302 |
PharmGKBi | PA33757 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1113, Eukaryota |
GeneTreei | ENSGT00940000154836 |
HOGENOMi | CLU_018310_2_0_1 |
InParanoidi | P13861 |
OMAi | IARCHRG |
PhylomeDBi | P13861 |
TreeFami | TF314920 |
Enzyme and pathway databases
PathwayCommonsi | P13861 |
Reactomei | R-HSA-163615, PKA activation R-HSA-164378, PKA activation in glucagon signalling R-HSA-180024, DARPP-32 events R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-HSA-5610787, Hedgehog 'off' state R-HSA-9010642, ROBO receptors bind AKAP5 R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-983231, Factors involved in megakaryocyte development and platelet production |
SignaLinki | P13861 |
SIGNORi | P13861 |
Miscellaneous databases
BioGRID-ORCSi | 5576, 34 hits in 844 CRISPR screens |
ChiTaRSi | PRKAR2A, human |
EvolutionaryTracei | P13861 |
GeneWikii | PRKAR2A |
GenomeRNAii | 5576 |
Pharosi | P13861, Tbio |
PROi | PR:P13861 |
RNActi | P13861, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114302, Expressed in buccal mucosa cell and 255 other tissues |
ExpressionAtlasi | P13861, baseline and differential |
Genevisiblei | P13861, HS |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits |
DisProti | DP02333 |
Gene3Di | 2.60.120.10, 2 hits |
IDEALi | IID00411 |
InterProi | View protein in InterPro IPR012198, cAMP_dep_PK_reg_su IPR003117, cAMP_dep_PK_reg_su_I/II_a/b IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF02197, RIIa, 1 hit |
PIRSFi | PIRSF000548, PK_regulatory, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00394, RIIa, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits |
PROSITEi | View protein in PROSITE PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAP2_HUMAN | |
Accessioni | P13861Primary (citable) accession number: P13861 Secondary accession number(s): Q16823, Q9BUB1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 210 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families