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Protein

cAMP-dependent protein kinase type II-alpha regulatory subunit

Gene

PRKAR2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208cAMP 11
Binding sitei217cAMP 11
Binding sitei338cAMP 21
Binding sitei347cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 260cAMP 1Add BLAST122
Nucleotide bindingi261 – 404cAMP 2Add BLAST144

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-163615 PKA activation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-180024 DARPP-32 events
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-5610787 Hedgehog 'off' state
R-HSA-9010642 ROBO receptors bind AKAP5
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiP13861
SIGNORiP13861

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type II-alpha regulatory subunit
Gene namesi
Name:PRKAR2A
Synonyms:PKR2, PRKAR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114302.15
HGNCiHGNC:9391 PRKAR2A
MIMi176910 gene
neXtProtiNX_P13861

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5576
OpenTargetsiENSG00000114302
PharmGKBiPA33757

Chemistry databases

DrugBankiDB05798 GEM-231
GuidetoPHARMACOLOGYi1474

Polymorphism and mutation databases

BioMutaiPRKAR2A
DMDMi125198

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002053852 – 404cAMP-dependent protein kinase type II-alpha regulatory subunitAdd BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei48PhosphoserineCombined sources1
Modified residuei54PhosphothreonineCombined sources1
Modified residuei58PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei80PhosphoserineCombined sources1
Modified residuei99Phosphoserine; by PKACombined sources1
Modified residuei215Phosphothreonine; by PDPK1By similarity1
Modified residuei350PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the activated catalytic chain.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP13861
MaxQBiP13861
PaxDbiP13861
PeptideAtlasiP13861
PRIDEiP13861
ProteomicsDBi52996

2D gel databases

OGPiP13861

PTM databases

iPTMnetiP13861
PhosphoSitePlusiP13861

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSG00000114302 Expressed in 243 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_PRKAR2A
ExpressionAtlasiP13861 baseline and differential
GenevisibleiP13861 HS

Organism-specific databases

HPAiCAB005023
HPA038268

Interactioni

Subunit structurei

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (PubMed:27484798).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111562, 71 interactors
CORUMiP13861
DIPiDIP-552N
IntActiP13861, 64 interactors
MINTiP13861
STRINGi9606.ENSP00000265563

Structurei

Secondary structure

1404
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13861
SMRiP13861
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13861

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 138Dimerization and phosphorylationAdd BLAST137

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196668
HOVERGENiHBG002025
InParanoidiP13861
KOiK04739
OMAiKTDQQRC
OrthoDBiEOG091G0F1K
PhylomeDBiP13861
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P13861-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSHIQIPPGL TELLQGYTVE VLRQQPPDLV EFAVEYFTRL REARAPASVL
60 70 80 90 100
PAATPRQSLG HPPPEPGPDR VADAKGDSES EEDEDLEVPV PSRFNRRVSV
110 120 130 140 150
CAETYNPDEE EEDTDPRVIH PKTDEQRCRL QEACKDILLF KNLDQEQLSQ
160 170 180 190 200
VLDAMFERIV KADEHVIDQG DDGDNFYVIE RGTYDILVTK DNQTRSVGQY
210 220 230 240 250
DNRGSFGELA LMYNTPRAAT IVATSEGSLW GLDRVTFRRI IVKNNAKKRK
260 270 280 290 300
MFESFIESVP LLKSLEVSER MKIVDVIGEK IYKDGERIIT QGEKADSFYI
310 320 330 340 350
IESGEVSILI RSRTKSNKDG GNQEVEIARC HKGQYFGELA LVTNKPRAAS
360 370 380 390 400
AYAVGDVKCL VMDVQAFERL LGPCMDIMKR NISHYEEQLV KMFGSSVDLG

NLGQ
Length:404
Mass (Da):45,518
Last modified:January 23, 2007 - v2
Checksum:iC7D6E8E476E1DB65
GO
Isoform 2 (identifier: P13861-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-313: Missing.

Show »
Length:382
Mass (Da):43,067
Checksum:i87D6AC1A5CE9E300
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J830C9J830_HUMAN
cAMP-dependent protein kinase type ...
PRKAR2A
114Annotation score:
H7C1L0H7C1L0_HUMAN
cAMP-dependent protein kinase type ...
PRKAR2A
200Annotation score:
H7C330H7C330_HUMAN
cAMP-dependent protein kinase type ...
PRKAR2A
92Annotation score:
H7C4A9H7C4A9_HUMAN
cAMP-dependent protein kinase type ...
PRKAR2A
99Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056821292 – 313Missing in isoform 2. 2 PublicationsAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14968 mRNA Translation: CAA33094.1
BT007225 mRNA Translation: AAP35889.1
AC141002 Genomic DNA No translation available.
AC144546 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW64926.1
CH471055 Genomic DNA Translation: EAW64927.1
BC002763 mRNA Translation: AAH02763.1
X99455 Genomic DNA Translation: CAA67817.1
CCDSiCCDS2778.1 [P13861-1]
CCDS82771.1 [P13861-2]
PIRiS03885 OKHU2R
RefSeqiNP_001308911.1, NM_001321982.1 [P13861-1]
NP_001308912.1, NM_001321983.1 [P13861-2]
NP_004148.1, NM_004157.3 [P13861-1]
XP_011532244.1, XM_011533942.2 [P13861-1]
XP_016862304.1, XM_017006815.1
UniGeneiHs.631923
Hs.731573

Genome annotation databases

EnsembliENST00000265563; ENSP00000265563; ENSG00000114302 [P13861-1]
ENST00000296446; ENSP00000296446; ENSG00000114302 [P13861-2]
ENST00000454963; ENSP00000394041; ENSG00000114302 [P13861-1]
GeneIDi5576
KEGGihsa:5576
UCSCiuc003cux.2 human [P13861-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14968 mRNA Translation: CAA33094.1
BT007225 mRNA Translation: AAP35889.1
AC141002 Genomic DNA No translation available.
AC144546 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW64926.1
CH471055 Genomic DNA Translation: EAW64927.1
BC002763 mRNA Translation: AAH02763.1
X99455 Genomic DNA Translation: CAA67817.1
CCDSiCCDS2778.1 [P13861-1]
CCDS82771.1 [P13861-2]
PIRiS03885 OKHU2R
RefSeqiNP_001308911.1, NM_001321982.1 [P13861-1]
NP_001308912.1, NM_001321983.1 [P13861-2]
NP_004148.1, NM_004157.3 [P13861-1]
XP_011532244.1, XM_011533942.2 [P13861-1]
XP_016862304.1, XM_017006815.1
UniGeneiHs.631923
Hs.731573

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IZXX-ray1.30A/B4-44[»]
2KYGNMR-A/B1-45[»]
4ZP3X-ray2.63A/B/C/D/E/F/G/H/I/J/K/L2-44[»]
5H78X-ray2.00A/B5-49[»]
5XBYX-ray3.25A/B/C/D5-44[»]
ProteinModelPortaliP13861
SMRiP13861
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111562, 71 interactors
CORUMiP13861
DIPiDIP-552N
IntActiP13861, 64 interactors
MINTiP13861
STRINGi9606.ENSP00000265563

Chemistry databases

DrugBankiDB05798 GEM-231
GuidetoPHARMACOLOGYi1474

PTM databases

iPTMnetiP13861
PhosphoSitePlusiP13861

Polymorphism and mutation databases

BioMutaiPRKAR2A
DMDMi125198

2D gel databases

OGPiP13861

Proteomic databases

EPDiP13861
MaxQBiP13861
PaxDbiP13861
PeptideAtlasiP13861
PRIDEiP13861
ProteomicsDBi52996

Protocols and materials databases

DNASUi5576
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265563; ENSP00000265563; ENSG00000114302 [P13861-1]
ENST00000296446; ENSP00000296446; ENSG00000114302 [P13861-2]
ENST00000454963; ENSP00000394041; ENSG00000114302 [P13861-1]
GeneIDi5576
KEGGihsa:5576
UCSCiuc003cux.2 human [P13861-1]

Organism-specific databases

CTDi5576
DisGeNETi5576
EuPathDBiHostDB:ENSG00000114302.15
GeneCardsiPRKAR2A
HGNCiHGNC:9391 PRKAR2A
HPAiCAB005023
HPA038268
MIMi176910 gene
neXtProtiNX_P13861
OpenTargetsiENSG00000114302
PharmGKBiPA33757
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196668
HOVERGENiHBG002025
InParanoidiP13861
KOiK04739
OMAiKTDQQRC
OrthoDBiEOG091G0F1K
PhylomeDBiP13861
TreeFamiTF314920

Enzyme and pathway databases

ReactomeiR-HSA-163615 PKA activation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-180024 DARPP-32 events
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-5610787 Hedgehog 'off' state
R-HSA-9010642 ROBO receptors bind AKAP5
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiP13861
SIGNORiP13861

Miscellaneous databases

ChiTaRSiPRKAR2A human
EvolutionaryTraceiP13861
GeneWikiiPRKAR2A
GenomeRNAii5576
PROiPR:P13861
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114302 Expressed in 243 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_PRKAR2A
ExpressionAtlasiP13861 baseline and differential
GenevisibleiP13861 HS

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKAP2_HUMAN
AccessioniPrimary (citable) accession number: P13861
Secondary accession number(s): Q16823, Q9BUB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 194 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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