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Protein

Acylamino-acid-releasing enzyme

Gene

APEH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.2 Publications

Catalytic activityi

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei587Charge relay systemPROSITE-ProRule annotation1 Publication1
Active sitei675Charge relay systemPROSITE-ProRule annotation1
Active sitei707Charge relay systemPROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • omega peptidase activity Source: UniProtKB
  • RNA binding Source: UniProtKB
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciMetaCyc:HS08997-MONOMER
ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-72764 Eukaryotic Translation Termination

Protein family/group databases

ESTHERihuman-APEH ACPH_Peptidase_S9
MEROPSiS09.004

Names & Taxonomyi

Protein namesi
Recommended name:
Acylamino-acid-releasing enzyme (EC:3.4.19.1)
Short name:
AARE
Alternative name(s):
Acyl-peptide hydrolase
Short name:
APH
Acylaminoacyl-peptidase
Oxidized protein hydrolase
Short name:
OPH
Gene namesi
Name:APEH
Synonyms:D3F15S2, D3S48E, DNF15S2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000164062.12
HGNCiHGNC:586 APEH
MIMi102645 gene
neXtProtiNX_P13798

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi327
OpenTargetsiENSG00000164062
PharmGKBiPA24878

Chemistry databases

ChEMBLiCHEMBL1741174
GuidetoPHARMACOLOGYi2328

Polymorphism and mutation databases

BioMutaiAPEH
DMDMi38258902

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224301 – 732Acylamino-acid-releasing enzymeAdd BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei185PhosphoserineCombined sources1
Modified residuei187PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP13798
MaxQBiP13798
PaxDbiP13798
PeptideAtlasiP13798
PRIDEiP13798
ProteomicsDBi52988

PTM databases

iPTMnetiP13798
PhosphoSitePlusiP13798

Expressioni

Gene expression databases

BgeeiENSG00000164062 Expressed in 211 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_APEH
ExpressionAtlasiP13798 baseline and differential
GenevisibleiP13798 HS

Organism-specific databases

HPAiHPA029700
HPA029701
HPA029702
HPA029703

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106824, 41 interactors
IntActiP13798, 5 interactors
STRINGi9606.ENSP00000296456

Structurei

3D structure databases

ProteinModelPortaliP13798
SMRiP13798
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9C family.Curated

Phylogenomic databases

eggNOGiKOG2100 Eukaryota
COG1506 LUCA
GeneTreeiENSGT00390000013172
HOGENOMiHOG000007443
HOVERGENiHBG000869
InParanoidiP13798
KOiK01303
PhylomeDBiP13798
TreeFamiTF312937

Family and domain databases

Gene3Di2.120.10.30, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR029058 AB_hydrolase
IPR002471 Pept_S9_AS
IPR001375 Peptidase_S9
PfamiView protein in Pfam
PF00326 Peptidase_S9, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00708 PRO_ENDOPEP_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.iShow all

P13798-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERQVLLSEP EEAAALYRGL SRQPALSAAC LGPEVTTQYG GQYRTVHTEW
60 70 80 90 100
TQRDLERMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG
110 120 130 140 150
TMKAVLRKAG GTGPGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF
160 170 180 190 200
GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDVSASDDE IARLKKPDQA
210 220 230 240 250
IKGDQFVFYE DWGENMVSKS IPVLCVLDVE SGNISVLEGV PENVSPGQAF
260 270 280 290 300
WAPGDAGVVF VGWWHEPFRL GIRFCTNRRS ALYYVDLIGG KCELLSDDSL
310 320 330 340 350
AVSSPRLSPD QCRIVYLQYP SLIPHHQCSQ LCLYDWYTKV TSVVVDVVPR
360 370 380 390 400
QLGENFSGIY CSLLPLGCWS ADSQRVVFDS AQRSRQDLFA VDTQVGTVTS
410 420 430 440 450
LTAGGSGGSW KLLTIDQDLM VAQFSTPSLP PTLKVGFLPS AGKEQSVLWV
460 470 480 490 500
SLEEAEPIPD IHWGIRVLQP PPEQENVQYA GLDFEAILLQ PGSPPDKTQV
510 520 530 540 550
PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS TGFGQDSILS
560 570 580 590 600
LPGNVGHQDV KDVQFAVEQV LQEEHFDASH VALMGGSHGG FISCHLIGQY
610 620 630 640 650
PETYRACVAR NPVINIASML GSTDIPDWCV VEAGFPFSSD CLPDLSVWAE
660 670 680 690 700
MLDKSPIRYI PQVKTPLLLM LGQEDRRVPF KQGMEYYRAL KTRNVPVRLL
710 720 730
LYPKSTHALS EVEVESDSFM NAVLWLRTHL GS
Length:732
Mass (Da):81,225
Last modified:November 7, 2003 - v4
Checksum:iA2C370516324D851
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JIF9C9JIF9_HUMAN
Acylamino-acid-releasing enzyme
APEH
737Annotation score:
H7C393H7C393_HUMAN
Acylamino-acid-releasing enzyme
APEH
284Annotation score:
H7C1U0H7C1U0_HUMAN
Acylamino-acid-releasing enzyme
APEH
308Annotation score:
C9JLK2C9JLK2_HUMAN
Acylamino-acid-releasing enzyme
APEH
232Annotation score:
H0YFE5H0YFE5_HUMAN
Acylamino-acid-releasing enzyme
APEH
47Annotation score:
F8WEH5F8WEH5_HUMAN
Acylamino-acid-releasing enzyme
APEH
50Annotation score:
H7C273H7C273_HUMAN
Acylamino-acid-releasing enzyme
APEH
54Annotation score:
F8WBC4F8WBC4_HUMAN
Acylamino-acid-releasing enzyme
APEH
58Annotation score:

Sequence cautioni

The sequence AAA35769 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101T → S in BAA07476 (PubMed:8724851).Curated1
Sequence conflicti137A → V in BAA07476 (PubMed:8724851).Curated1
Sequence conflicti168K → R in BAA07476 (PubMed:8724851).Curated1
Sequence conflicti200A → P in BAA07476 (PubMed:8724851).Curated1
Sequence conflicti403A → V in AAF37321 (PubMed:10719179).Curated1

Mass spectrometryi

Molecular mass is 81269.9±8.7 Da from positions 1 - 732. Determined by ESI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051580541T → M. Corresponds to variant dbSNP:rs3816877Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38441 mRNA Translation: BAA07476.1
AF141383 mRNA Translation: AAF37321.1
BC000362 mRNA Translation: AAH00362.1
BC001499 mRNA Translation: AAH01499.1
BC001826 mRNA Translation: AAH01826.1
J03068 mRNA Translation: AAA35769.1 Frameshift.
CCDSiCCDS2801.1
PIRiJC4655
RefSeqiNP_001631.3, NM_001640.3
XP_011531962.1, XM_011533660.2
UniGeneiHs.517969

Genome annotation databases

EnsembliENST00000296456; ENSP00000296456; ENSG00000164062
GeneIDi327
KEGGihsa:327
UCSCiuc003cxf.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38441 mRNA Translation: BAA07476.1
AF141383 mRNA Translation: AAF37321.1
BC000362 mRNA Translation: AAH00362.1
BC001499 mRNA Translation: AAH01499.1
BC001826 mRNA Translation: AAH01826.1
J03068 mRNA Translation: AAA35769.1 Frameshift.
CCDSiCCDS2801.1
PIRiJC4655
RefSeqiNP_001631.3, NM_001640.3
XP_011531962.1, XM_011533660.2
UniGeneiHs.517969

3D structure databases

ProteinModelPortaliP13798
SMRiP13798
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106824, 41 interactors
IntActiP13798, 5 interactors
STRINGi9606.ENSP00000296456

Chemistry databases

ChEMBLiCHEMBL1741174
GuidetoPHARMACOLOGYi2328

Protein family/group databases

ESTHERihuman-APEH ACPH_Peptidase_S9
MEROPSiS09.004

PTM databases

iPTMnetiP13798
PhosphoSitePlusiP13798

Polymorphism and mutation databases

BioMutaiAPEH
DMDMi38258902

Proteomic databases

EPDiP13798
MaxQBiP13798
PaxDbiP13798
PeptideAtlasiP13798
PRIDEiP13798
ProteomicsDBi52988

Protocols and materials databases

DNASUi327
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296456; ENSP00000296456; ENSG00000164062
GeneIDi327
KEGGihsa:327
UCSCiuc003cxf.4 human

Organism-specific databases

CTDi327
DisGeNETi327
EuPathDBiHostDB:ENSG00000164062.12
GeneCardsiAPEH
HGNCiHGNC:586 APEH
HPAiHPA029700
HPA029701
HPA029702
HPA029703
MIMi102645 gene
neXtProtiNX_P13798
OpenTargetsiENSG00000164062
PharmGKBiPA24878
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2100 Eukaryota
COG1506 LUCA
GeneTreeiENSGT00390000013172
HOGENOMiHOG000007443
HOVERGENiHBG000869
InParanoidiP13798
KOiK01303
PhylomeDBiP13798
TreeFamiTF312937

Enzyme and pathway databases

BioCyciMetaCyc:HS08997-MONOMER
ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-72764 Eukaryotic Translation Termination

Miscellaneous databases

ChiTaRSiAPEH human
GeneWikiiAPEH_(gene)
GenomeRNAii327
PROiPR:P13798
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164062 Expressed in 211 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_APEH
ExpressionAtlasiP13798 baseline and differential
GenevisibleiP13798 HS

Family and domain databases

Gene3Di2.120.10.30, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR029058 AB_hydrolase
IPR002471 Pept_S9_AS
IPR001375 Peptidase_S9
PfamiView protein in Pfam
PF00326 Peptidase_S9, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00708 PRO_ENDOPEP_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACPH_HUMAN
AccessioniPrimary (citable) accession number: P13798
Secondary accession number(s): Q9BQ33, Q9P0Y2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 7, 2003
Last modified: September 12, 2018
This is version 180 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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