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Protein

ATP-dependent target DNA activator B

Gene

B

Organism
Escherichia phage D108 (Bacteriophage D108)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Selects the target DNA sites for transposition. Recruits DDE-recombinase A to the target sites and catalytically activates it. Displays non-specific DNA-binding properties. Polymerizes as helical filaments around the DNA. Coating of the DNA by the target DNA activator B might play a role in favoring target-primed replication over integration. Prevents self-integration into an integrated copy of the viral genome. This mechanism is called target immunity and is achieved by two mechanisms: first, the target DNA activator B dissociates from the viral genome ends upon interaction in cis with DDE-recombinase A, which makes the viral genome ends a poor target for new insertions. Second, the interior of the viral genome may also ne protected from integration events by the target DNA activator B being strongly bound throughout the whole viral genome (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase
Biological processDNA integration, DNA replication, Transposition, Viral DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent target DNA activator B (EC:3.6.1.3)
Alternative name(s):
Gene product B
Short name:
gpB
MuB
Gene namesi
Name:B
OrganismiEscherichia phage D108 (Bacteriophage D108)
Taxonomic identifieri665033 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMuvirusunclassified Mu-like viruses
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000320 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000776781 – 312ATP-dependent target DNA activator BAdd BLAST312

Expressioni

Inductioni

Expressed in the early phase of the viral replicative cycle. Expression of early genes is repressed by viral Repc (latency) and favored by viral Ner protein.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homomultimer. Polymerizes in presence of ATP, preferentially on DNA. ATP hydrolysis triggers polymers dissassembly. Interacts with DDE-recombinase A; this interaction stimulates the catalytic activity of the latter as well as the ATPase activity of MuB followed by its dissociation from DNA (By similarity).By similarity

Family & Domainsi

Domaini

The N-terminus contains the DNA-binding region required for target capture and an AAA+ ATPase domain. The C-terminus comprises four helices arranged in a loosely packed bundle which can also bind to dsDNA (By similarity).By similarity

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

KOiK07132
OrthoDBiVOG090002CQ

Family and domain databases

Gene3Di1.10.1180.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR036733 B_transposit_C_sf
IPR009084 B_transpositn_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF13401 AAA_22, 1 hit
PF09077 Phage-MuB_C, 1 hit
SUPFAMiSSF47681 SSF47681, 1 hit
SSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

P13771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNISDIRAGL RTLVENEETT FKQIALESGL STGTISSFIN DKYNGDNERV
60 70 80 90 100
SQMLQRWLEK YHAVAELPEP PRFVETQTVK QIWTSMRFAS LTESIAVVCG
110 120 130 140 150
NPGVGKTEAA REYRRTNNNV WMITITPSCA SVLECLTELA FELGMNDAPR
160 170 180 190 200
RKGPLSRALR RRLEGTQGLV IIDEADHLGA EVLEELRLLQ ESTRTGLVLM
210 220 230 240 250
GNHRVYSNMT GGNRTVEFAR LFSRIAKRTA INKTKKADVK AIADAWQING
260 270 280 290 300
ENELELLQQI AQKPGALRIL NHSLRLAAMT AHGKGERVNE DYLRQAFREL
310
DLDVDISTLL RN
Length:312
Mass (Da):35,080
Last modified:June 11, 2014 - v2
Checksum:i3F1455A1C72AFF44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18902 Genomic DNA Translation: AAA32205.1
GQ357916 Genomic DNA Translation: ACV50263.1
RefSeqiYP_003335752.1, NC_013594.1

Genome annotation databases

GeneIDi8658815
KEGGivg:8658815

Similar proteinsi

Entry informationi

Entry nameiTARGB_BPD10
AccessioniPrimary (citable) accession number: P13771
Secondary accession number(s): C9DGL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 11, 2014
Last modified: April 25, 2018
This is version 57 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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