UniProtKB - P13738 (NHAA_ECOLI)

BLAST

>sp|P13738|NHAA_ECOLI Na(+)/H(+) antiporter NhaA OS=Escherichia coli (strain K12) OX=83333 GN=nhaA PE=1 SV=3
MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLETPVQLRVGSLEINKNML
LWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAIGGMIVPALLYLAFNYADP
ITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALAIIDDLGAIIIIALFYTNDLS
MASLGVAAVAIAVLAVLNLCGARRTGVYILVGVVLWTAVLKSGVHATLAGVIVGFFIPLK
EKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSLQGVTLDGLTSILPLGIIAGLLIGK
PLGISLFCWLALRLKLAHLPEGTTYQQIMVVGILCGIGFTMSIFIASLAFGSVDPELINW
AKLGILVGSISSAVIGYSWLRVRLRPSV

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History
Entry version 163 (07 Nov 2018)
Sequence version 3 (01 Feb 1996)
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Protein

Na(+)/H(+) antiporter NhaA

Gene

nhaA

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Na⁺/H⁺ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H⁺ per Na⁺. Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	133	Essential for antiport activity	1
Siteⁱ	163 – 164	Essential for antiport activity	2
Siteⁱ	163	Essential for antiport activity	1
Siteⁱ	241	Important for pH sensorCurated	1
Siteⁱ	249	Important for pH sensorCurated	1
Siteⁱ	252	Important for pH sensorCurated	1

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular sodium ion homeostasis
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 2555351
regulation of intracellular pH
Source: EcoCyc
Inferred from direct assayⁱ
- 1645730
response to cation stress
Source: EcoCyc
Inferred from expression patternⁱ
- 1657980
sodium ion export across plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 1645730

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Antiport, Ion transport, Sodium transport, Transport
Ligand	Sodium

Enzyme and pathway databases

BioCycⁱ	EcoCyc:NHAA-MONOMER MetaCyc:NHAA-MONOMER

BioCycⁱ

EcoCyc:NHAA-MONOMER
MetaCyc:NHAA-MONOMER

Protein family/group databases

TCDBⁱ	2.A.33.1.1 the nhaa na(+):h(+) antiporter (nhaa) family

TCDBⁱ

2.A.33.1.1 the nhaa na(+):h(+) antiporter (nhaa) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Na(+)/H(+) antiporter NhaA Alternative name(s): Sodium/proton antiporter NhaA
Gene namesⁱ	Name:nhaA Synonyms:ant Ordered Locus Names:b0019, JW0018
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10652 nhaA

EcoGeneⁱ

EG10652 nhaA

Subcellular locationⁱ

Cell inner membrane ; Multi-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 11	Cytoplasmic1 PublicationAdd BLAST	11
Transmembraneⁱ	12 – 30	Helical; Name=1Add BLAST	19
Topological domainⁱ	31 – 58	Periplasmic1 PublicationAdd BLAST	28
Transmembraneⁱ	59 – 85	Helical; Name=2Add BLAST	27
Topological domainⁱ	86 – 94	Cytoplasmic1 Publication	9
Transmembraneⁱ	95 – 116	Helical; Name=3Add BLAST	22
Topological domainⁱ	117 – 120	Periplasmic1 Publication	4
Transmembraneⁱ	121 – 143	Helical; Name=4Add BLAST	23
Topological domainⁱ	144 – 149	Cytoplasmic1 Publication	6
Transmembraneⁱ	150 – 175	Helical; Name=5Add BLAST	26
Topological domainⁱ	176 – 181	Periplasmic1 Publication	6
Transmembraneⁱ	182 – 200	Helical; Name=6Add BLAST	19
Topological domainⁱ	201 – 204	Cytoplasmic1 Publication	4
Transmembraneⁱ	205 – 218	Helical; Name=7Add BLAST	14
Topological domainⁱ	219 – 222	Periplasmic1 Publication	4
Transmembraneⁱ	223 – 236	Helical; Name=8Add BLAST	14
Topological domainⁱ	237 – 246	Cytoplasmic1 Publication	10
Transmembraneⁱ	247 – 271	Helical; Name=9Add BLAST	25
Topological domainⁱ	272 – 289	Periplasmic1 PublicationAdd BLAST	18
Transmembraneⁱ	290 – 311	Helical; Name=10Add BLAST	22
Topological domainⁱ	312 – 326	Cytoplasmic1 PublicationAdd BLAST	15
Transmembraneⁱ	327 – 350	Helical; Name=11Add BLAST	24
Topological domainⁱ	351 – 356	Periplasmic1 Publication	6
Transmembraneⁱ	357 – 382	Helical; Name=12Add BLAST	26
Topological domainⁱ	383 – 388	Cytoplasmic1 Publication	6

GO - Cellular componentⁱ

integral component of plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 8943289
plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 15919996

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	133	D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication	1
Mutagenesisⁱ	163	D → C: Loss of antiport activity. 2 Publications	1
Mutagenesisⁱ	163	D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 2 Publications	1
Mutagenesisⁱ	164	D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication	1
Mutagenesisⁱ	225	H → A: Loss of antiport activity. Abolishes ability to grow at alkaline pH. 2 Publications	1
Mutagenesisⁱ	225	H → C or S: Slightly reduced antiport activity. No effect on pH sensitivity. 2 Publications	1
Mutagenesisⁱ	225	H → D or R: Shifts threshold for pH-sensitive inactivation. 2 Publications	1
Mutagenesisⁱ	338	G → S: Loss of pH-sensitivity. 1 Publication	1

Chemistry databases

ChEMBLⁱ	CHEMBL5478

ChEMBLⁱ

CHEMBL5478

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000052410	1 – 388	Na(+)/H(+) antiporter NhaAAdd BLAST		388

Proteomic databases

PaxDbⁱ	P13738
PRIDEⁱ	P13738

Expressionⁱ

Inductionⁱ

Transcription stimulated by high Na⁺ concentrations.

Interactionⁱ

Subunit structureⁱ

Monomer and homodimer.2 Publications

Protein-protein interaction databases

BioGridⁱ	4259720, 41 interactors
Database of interacting proteins More...DIPⁱ	DIP-10335N
STRINGⁱ	316385.ECDH10B_0020

Chemistry databases

BindingDBⁱ

P13738

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	13 – 30	Combined sources	18
Beta strandⁱ	32 – 34	Combined sources	3
Helixⁱ	35 – 41	Combined sources	7
Beta strandⁱ	45 – 50	Combined sources	6
Beta strandⁱ	53 – 58	Combined sources	6
Helixⁱ	59 – 84	Combined sources	26
Turnⁱ	86 – 88	Combined sources	3
Turnⁱ	91 – 93	Combined sources	3
Helixⁱ	95 – 104	Combined sources	10
Turnⁱ	105 – 108	Combined sources	4
Helixⁱ	109 – 112	Combined sources	4
Helixⁱ	113 – 115	Combined sources	3
Helixⁱ	122 – 125	Combined sources	4
Beta strandⁱ	127 – 130	Combined sources	4
Helixⁱ	134 – 142	Combined sources	9
Beta strandⁱ	146 – 148	Combined sources	3
Beta strandⁱ	150 – 152	Combined sources	3
Helixⁱ	153 – 174	Combined sources	22
Helixⁱ	181 – 199	Combined sources	19
Helixⁱ	206 – 218	Combined sources	13
Turnⁱ	219 – 221	Combined sources	3
Helixⁱ	223 – 236	Combined sources	14
Helixⁱ	241 – 243	Combined sources	3
Helixⁱ	247 – 261	Combined sources	15
Helixⁱ	263 – 271	Combined sources	9
Beta strandⁱ	277 – 279	Combined sources	3
Helixⁱ	284 – 287	Combined sources	4
Turnⁱ	290 – 293	Combined sources	4
Helixⁱ	294 – 298	Combined sources	5
Helixⁱ	301 – 303	Combined sources	3
Turnⁱ	305 – 311	Combined sources	7
Turnⁱ	315 – 317	Combined sources	3
Helixⁱ	325 – 328	Combined sources	4
Helixⁱ	331 – 334	Combined sources	4
Helixⁱ	339 – 349	Combined sources	11
Turnⁱ	351 – 353	Combined sources	3
Helixⁱ	358 – 370	Combined sources	13
Turnⁱ	375 – 377	Combined sources	3
Helixⁱ	378 – 380	Combined sources	3
Turnⁱ	381 – 383	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P13738
SMRⁱ	P13738
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P13738

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	45 – 58	Important for dimerizationAdd BLAST		14

Sequence similaritiesⁱ

Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105DRC Bacteria COG3004 LUCA
HOGENOMⁱ	HOG000218489
InParanoidⁱ	P13738
KEGG Orthology (KO) More...KOⁱ	K03313
PhylomeDBⁱ	P13738

Family and domain databases

Gene3Dⁱ	1.20.1530.10, 1 hit
HAMAPⁱ	MF_01844 NhaA, 1 hit
InterProⁱ	View protein in InterPro IPR023171 Na/H_antiporter_dom_sf IPR004670 NhaA
PANTHERⁱ	PTHR30341 PTHR30341, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06965 Na_H_antiport_1, 1 hit
TIGRFAMsⁱ	TIGR00773 NhaA, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P13738-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV 
        60         70         80         90        100
GSLEINKNML LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA 
       110        120        130        140        150
AIGGMIVPAL LYLAFNYADP ITREGWAIPA ATDIAFALGV LALLGSRVPL 
       160        170        180        190        200
ALKIFLMALA IIDDLGAIII IALFYTNDLS MASLGVAAVA IAVLAVLNLC 
       210        220        230        240        250
GARRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK EKHGRSPAKR 
       260        270        280        290        300
LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK 
       310        320        330        340        350
PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF 
       360        370        380 
GSVDPELINW AKLGILVGSI SSAVIGYSWL RVRLRPSV

Length:388

Mass (Da):41,356

Last modified:February 1, 1996 - v3

Checksum:ⁱB508B1D2E5EE9130

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J03879 Genomic DNA Translation: AAA23448.2 U00096 Genomic DNA Translation: AAC73130.1 AP009048 Genomic DNA Translation: BAB96592.1 S67239 Genomic DNA Translation: AAB20348.1
PIRⁱ	C64722
NCBI Reference Sequences More...RefSeqⁱ	NP_414560.1, NC_000913.3 WP_000681354.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73130; AAC73130; b0019 BAB96592; BAB96592; BAB96592
GeneIDⁱ	944758
KEGGⁱ	ecj:JW0018 eco:b0019
PATRICⁱ	fig\|1411691.4.peg.2265

Protein	Similar proteins		Species	Score	Length	Source
P13738	Na+/H+ antiporter NhaA		Shigella sp.		388	UniRef100_P13738
Na(+)/H(+) antiporter NhaA		uncultured bacterium		388
Na(+)/H(+) antiporter NhaA		PSEST		388
Na+/H+ antiporter A (Fragment)		ENTFC		388
Na(+)/H(+) antiporter NhaA		ECOLX		388
+3

Protein	Similar proteins		Species	Score	Length	Source
P13738	Na(+)/H(+) antiporter NhaA		ECO24		388	UniRef90_P13738
Na(+)/H(+) antiporter NhaA		ECOHS		388
Na(+)/H(+) antiporter NhaA		SHIFL		388
Na(+)/H(+) antiporter NhaA		CITK8		388
Na(+)/H(+) antiporter NhaA		SHIF8		388
+781

Protein	Similar proteins		Species	Score	Length	Source
P13738	Na(+)/H(+) antiporter NhaA		SALCH		388	UniRef50_P13738
Na(+)/H(+) antiporter NhaA		SALPB		388
Na(+)/H(+) antiporter NhaA		SALTY		388
Na(+)/H(+) antiporter NhaA		SHIF8		388
Na(+)/H(+) antiporter NhaA		CITK8		388
+1571

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J03879 Genomic DNA Translation: AAA23448.2 U00096 Genomic DNA Translation: AAC73130.1 AP009048 Genomic DNA Translation: BAB96592.1 S67239 Genomic DNA Translation: AAB20348.1
PIRⁱ	C64722
RefSeqⁱ	NP_414560.1, NC_000913.3 WP_000681354.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1ZCD	X-ray	3.45	A/B	1-388	[»]
	3FI1	electron microscopy	7.00	A	9-384	[»]
	4ATV	X-ray	3.50	A/B/C/D	1-388	[»]
	4AU5	X-ray	3.70	A/B/C/D	1-388	[»]
ProteinModelPortalⁱ	P13738
SMRⁱ	P13738
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259720, 41 interactors
DIPⁱ	DIP-10335N
STRINGⁱ	316385.ECDH10B_0020

Chemistry databases

BindingDBⁱ	P13738
ChEMBLⁱ	CHEMBL5478

Protein family/group databases

TCDBⁱ	2.A.33.1.1 the nhaa na(+):h(+) antiporter (nhaa) family

TCDBⁱ

2.A.33.1.1 the nhaa na(+):h(+) antiporter (nhaa) family

Proteomic databases

PaxDbⁱ	P13738
PRIDEⁱ	P13738

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73130; AAC73130; b0019 BAB96592; BAB96592; BAB96592
GeneIDⁱ	944758
KEGGⁱ	ecj:JW0018 eco:b0019
PATRICⁱ	fig\|1411691.4.peg.2265

Organism-specific databases

EchoBASEⁱ	EB0646
EcoGeneⁱ	EG10652 nhaA

Phylogenomic databases

eggNOGⁱ	ENOG4105DRC Bacteria COG3004 LUCA
HOGENOMⁱ	HOG000218489
InParanoidⁱ	P13738
KOⁱ	K03313
PhylomeDBⁱ	P13738

Enzyme and pathway databases

BioCycⁱ	EcoCyc:NHAA-MONOMER MetaCyc:NHAA-MONOMER

BioCycⁱ

EcoCyc:NHAA-MONOMER
MetaCyc:NHAA-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P13738
Protein Ontology More...PROⁱ	PR:P13738

Family and domain databases

Gene3Dⁱ	1.20.1530.10, 1 hit
HAMAPⁱ	MF_01844 NhaA, 1 hit
InterProⁱ	View protein in InterPro IPR023171 Na/H_antiporter_dom_sf IPR004670 NhaA
PANTHERⁱ	PTHR30341 PTHR30341, 1 hit
Pfamⁱ	View protein in Pfam PF06965 Na_H_antiport_1, 1 hit
TIGRFAMsⁱ	TIGR00773 NhaA, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NHAA_ECOLI
Accessionⁱ	P13738Primary (citable) accession number: P13738
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
	Last sequence update:	February 1, 1996
	Last modified:	November 7, 2018
	This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

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Supporting data

UniProtKB - P13738 (NHAA_ECOLI)

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Na(+)/H(+) antiporter NhaA

nhaA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ