UniProtKB - P13738 (NHAA_ECOLI)
Protein
Na(+)/H(+) antiporter NhaA
Gene
nhaA
Organism
Escherichia coli (strain K12)
Status
Functioni
Na+/H+ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H+ per Na+. Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 133 | Essential for antiport activity | 1 | |
Sitei | 163 – 164 | Essential for antiport activity | 2 | |
Sitei | 163 | Essential for antiport activity | 1 | |
Sitei | 241 | Important for pH sensorCurated | 1 | |
Sitei | 249 | Important for pH sensorCurated | 1 | |
Sitei | 252 | Important for pH sensorCurated | 1 |
GO - Molecular functioni
- cardiolipin binding Source: EcoCyc
- sodium:proton antiporter activity Source: EcoCyc
GO - Biological processi
- cellular sodium ion homeostasis Source: EcoCyc
- regulation of intracellular pH Source: EcoCyc
- response to cation stress Source: EcoCyc
- sodium ion export across plasma membrane Source: EcoCyc
Keywordsi
Biological process | Antiport, Ion transport, Sodium transport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
BioCyci | EcoCyc:NHAA-MONOMER MetaCyc:NHAA-MONOMER |
Protein family/group databases
TCDBi | 2.A.33.1.1, the nhaa na(+):h(+) antiporter (nhaa) family |
Names & Taxonomyi
Protein namesi | Recommended name: Na(+)/H(+) antiporter NhaAAlternative name(s): Sodium/proton antiporter NhaA |
Gene namesi | Name:nhaA Synonyms:ant Ordered Locus Names:b0019, JW0018 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | Cytoplasmic1 PublicationAdd BLAST | 11 | |
Transmembranei | 12 – 30 | Helical; Name=1Add BLAST | 19 | |
Topological domaini | 31 – 58 | Periplasmic1 PublicationAdd BLAST | 28 | |
Transmembranei | 59 – 85 | Helical; Name=2Add BLAST | 27 | |
Topological domaini | 86 – 94 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 95 – 116 | Helical; Name=3Add BLAST | 22 | |
Topological domaini | 117 – 120 | Periplasmic1 Publication | 4 | |
Transmembranei | 121 – 143 | Helical; Name=4Add BLAST | 23 | |
Topological domaini | 144 – 149 | Cytoplasmic1 Publication | 6 | |
Transmembranei | 150 – 175 | Helical; Name=5Add BLAST | 26 | |
Topological domaini | 176 – 181 | Periplasmic1 Publication | 6 | |
Transmembranei | 182 – 200 | Helical; Name=6Add BLAST | 19 | |
Topological domaini | 201 – 204 | Cytoplasmic1 Publication | 4 | |
Transmembranei | 205 – 218 | Helical; Name=7Add BLAST | 14 | |
Topological domaini | 219 – 222 | Periplasmic1 Publication | 4 | |
Transmembranei | 223 – 236 | Helical; Name=8Add BLAST | 14 | |
Topological domaini | 237 – 246 | Cytoplasmic1 Publication | 10 | |
Transmembranei | 247 – 271 | Helical; Name=9Add BLAST | 25 | |
Topological domaini | 272 – 289 | Periplasmic1 PublicationAdd BLAST | 18 | |
Transmembranei | 290 – 311 | Helical; Name=10Add BLAST | 22 | |
Topological domaini | 312 – 326 | Cytoplasmic1 PublicationAdd BLAST | 15 | |
Transmembranei | 327 – 350 | Helical; Name=11Add BLAST | 24 | |
Topological domaini | 351 – 356 | Periplasmic1 Publication | 6 | |
Transmembranei | 357 – 382 | Helical; Name=12Add BLAST | 26 | |
Topological domaini | 383 – 388 | Cytoplasmic1 Publication | 6 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 133 | D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication | 1 | |
Mutagenesisi | 163 | D → C: Loss of antiport activity. 2 Publications | 1 | |
Mutagenesisi | 163 | D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 2 Publications | 1 | |
Mutagenesisi | 164 | D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication | 1 | |
Mutagenesisi | 225 | H → A: Loss of antiport activity. Abolishes ability to grow at alkaline pH. 2 Publications | 1 | |
Mutagenesisi | 225 | H → C or S: Slightly reduced antiport activity. No effect on pH sensitivity. 2 Publications | 1 | |
Mutagenesisi | 225 | H → D or R: Shifts threshold for pH-sensitive inactivation. 2 Publications | 1 | |
Mutagenesisi | 338 | G → S: Loss of pH-sensitivity. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:3266 |
Chemistry databases
ChEMBLi | CHEMBL5478 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000052410 | 1 – 388 | Na(+)/H(+) antiporter NhaAAdd BLAST | 388 |
Proteomic databases
jPOSTi | P13738 |
PaxDbi | P13738 |
PRIDEi | P13738 |
Expressioni
Inductioni
Transcription stimulated by high Na+ concentrations.
Interactioni
Subunit structurei
Monomer and homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259720, 41 interactors |
DIPi | DIP-10335N |
STRINGi | 511145.b0019 |
Chemistry databases
BindingDBi | P13738 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P13738 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13738 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 45 – 58 | Important for dimerizationAdd BLAST | 14 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3004, Bacteria |
HOGENOMi | CLU_015803_1_0_6 |
InParanoidi | P13738 |
PhylomeDBi | P13738 |
Family and domain databases
Gene3Di | 1.20.1530.10, 1 hit |
HAMAPi | MF_01844, NhaA, 1 hit |
InterProi | View protein in InterPro IPR023171, Na/H_antiporter_dom_sf IPR004670, NhaA |
PANTHERi | PTHR30341, PTHR30341, 1 hit |
Pfami | View protein in Pfam PF06965, Na_H_antiport_1, 1 hit |
TIGRFAMsi | TIGR00773, NhaA, 1 hit |
i Sequence
Sequence statusi: Complete.
P13738-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV
60 70 80 90 100
GSLEINKNML LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA
110 120 130 140 150
AIGGMIVPAL LYLAFNYADP ITREGWAIPA ATDIAFALGV LALLGSRVPL
160 170 180 190 200
ALKIFLMALA IIDDLGAIII IALFYTNDLS MASLGVAAVA IAVLAVLNLC
210 220 230 240 250
GARRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK EKHGRSPAKR
260 270 280 290 300
LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK
310 320 330 340 350
PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF
360 370 380
GSVDPELINW AKLGILVGSI SSAVIGYSWL RVRLRPSV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03879 Genomic DNA Translation: AAA23448.2 U00096 Genomic DNA Translation: AAC73130.1 AP009048 Genomic DNA Translation: BAB96592.1 S67239 Genomic DNA Translation: AAB20348.1 |
PIRi | C64722 |
RefSeqi | NP_414560.1, NC_000913.3 WP_000681354.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73130; AAC73130; b0019 BAB96592; BAB96592; BAB96592 |
GeneIDi | 944758 |
KEGGi | ecj:JW0018 eco:b0019 |
PATRICi | fig|1411691.4.peg.2265 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03879 Genomic DNA Translation: AAA23448.2 U00096 Genomic DNA Translation: AAC73130.1 AP009048 Genomic DNA Translation: BAB96592.1 S67239 Genomic DNA Translation: AAB20348.1 |
PIRi | C64722 |
RefSeqi | NP_414560.1, NC_000913.3 WP_000681354.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZCD | X-ray | 3.45 | A/B | 1-388 | [»] | |
3FI1 | electron microscopy | 7.00 | A | 9-384 | [»] | |
4ATV | X-ray | 3.50 | A/B/C/D | 1-388 | [»] | |
4AU5 | X-ray | 3.70 | A/B/C/D | 1-388 | [»] | |
SMRi | P13738 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259720, 41 interactors |
DIPi | DIP-10335N |
STRINGi | 511145.b0019 |
Chemistry databases
BindingDBi | P13738 |
ChEMBLi | CHEMBL5478 |
Protein family/group databases
TCDBi | 2.A.33.1.1, the nhaa na(+):h(+) antiporter (nhaa) family |
Proteomic databases
jPOSTi | P13738 |
PaxDbi | P13738 |
PRIDEi | P13738 |
Genome annotation databases
EnsemblBacteriai | AAC73130; AAC73130; b0019 BAB96592; BAB96592; BAB96592 |
GeneIDi | 944758 |
KEGGi | ecj:JW0018 eco:b0019 |
PATRICi | fig|1411691.4.peg.2265 |
Organism-specific databases
EchoBASEi | EB0646 |
Phylogenomic databases
eggNOGi | COG3004, Bacteria |
HOGENOMi | CLU_015803_1_0_6 |
InParanoidi | P13738 |
PhylomeDBi | P13738 |
Enzyme and pathway databases
BioCyci | EcoCyc:NHAA-MONOMER MetaCyc:NHAA-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P13738 |
PHI-basei | PHI:3266 |
PROi | PR:P13738 |
Family and domain databases
Gene3Di | 1.20.1530.10, 1 hit |
HAMAPi | MF_01844, NhaA, 1 hit |
InterProi | View protein in InterPro IPR023171, Na/H_antiporter_dom_sf IPR004670, NhaA |
PANTHERi | PTHR30341, PTHR30341, 1 hit |
Pfami | View protein in Pfam PF06965, Na_H_antiport_1, 1 hit |
TIGRFAMsi | TIGR00773, NhaA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NHAA_ECOLI | |
Accessioni | P13738Primary (citable) accession number: P13738 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 175 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families