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Protein

Na(+)/H(+) antiporter NhaA

Gene

nhaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Na+/H+ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H+ per Na+. Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei133Essential for antiport activity1
Sitei163 – 164Essential for antiport activity2
Sitei163Essential for antiport activity1
Sitei241Important for pH sensorCurated1
Sitei249Important for pH sensorCurated1
Sitei252Important for pH sensorCurated1

GO - Molecular functioni

  • cardiolipin binding Source: EcoCyc
  • sodium:proton antiporter activity Source: EcoCyc

GO - Biological processi

  • cellular sodium ion homeostasis Source: EcoCyc
  • regulation of intracellular pH Source: EcoCyc
  • response to cation stress Source: EcoCyc
  • sodium ion export across plasma membrane Source: EcoCyc

Keywordsi

Biological processAntiport, Ion transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

BioCyciEcoCyc:NHAA-MONOMER
MetaCyc:NHAA-MONOMER

Protein family/group databases

TCDBi2.A.33.1.1 the nhaa na(+):h(+) antiporter (nhaa) family

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) antiporter NhaA
Alternative name(s):
Sodium/proton antiporter NhaA
Gene namesi
Name:nhaA
Synonyms:ant
Ordered Locus Names:b0019, JW0018
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10652 nhaA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11Cytoplasmic1 PublicationAdd BLAST11
Transmembranei12 – 30Helical; Name=1Add BLAST19
Topological domaini31 – 58Periplasmic1 PublicationAdd BLAST28
Transmembranei59 – 85Helical; Name=2Add BLAST27
Topological domaini86 – 94Cytoplasmic1 Publication9
Transmembranei95 – 116Helical; Name=3Add BLAST22
Topological domaini117 – 120Periplasmic1 Publication4
Transmembranei121 – 143Helical; Name=4Add BLAST23
Topological domaini144 – 149Cytoplasmic1 Publication6
Transmembranei150 – 175Helical; Name=5Add BLAST26
Topological domaini176 – 181Periplasmic1 Publication6
Transmembranei182 – 200Helical; Name=6Add BLAST19
Topological domaini201 – 204Cytoplasmic1 Publication4
Transmembranei205 – 218Helical; Name=7Add BLAST14
Topological domaini219 – 222Periplasmic1 Publication4
Transmembranei223 – 236Helical; Name=8Add BLAST14
Topological domaini237 – 246Cytoplasmic1 Publication10
Transmembranei247 – 271Helical; Name=9Add BLAST25
Topological domaini272 – 289Periplasmic1 PublicationAdd BLAST18
Transmembranei290 – 311Helical; Name=10Add BLAST22
Topological domaini312 – 326Cytoplasmic1 PublicationAdd BLAST15
Transmembranei327 – 350Helical; Name=11Add BLAST24
Topological domaini351 – 356Periplasmic1 Publication6
Transmembranei357 – 382Helical; Name=12Add BLAST26
Topological domaini383 – 388Cytoplasmic1 Publication6

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication1
Mutagenesisi163D → C: Loss of antiport activity. 2 Publications1
Mutagenesisi163D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 2 Publications1
Mutagenesisi164D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication1
Mutagenesisi225H → A: Loss of antiport activity. Abolishes ability to grow at alkaline pH. 2 Publications1
Mutagenesisi225H → C or S: Slightly reduced antiport activity. No effect on pH sensitivity. 2 Publications1
Mutagenesisi225H → D or R: Shifts threshold for pH-sensitive inactivation. 2 Publications1
Mutagenesisi338G → S: Loss of pH-sensitivity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5478

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000524101 – 388Na(+)/H(+) antiporter NhaAAdd BLAST388

Proteomic databases

PaxDbiP13738
PRIDEiP13738

Expressioni

Inductioni

Transcription stimulated by high Na+ concentrations.

Interactioni

Subunit structurei

Monomer and homodimer.2 Publications

Protein-protein interaction databases

BioGridi4259720, 41 interactors
DIPiDIP-10335N
STRINGi316385.ECDH10B_0020

Chemistry databases

BindingDBiP13738

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13738
SMRiP13738
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13738

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 58Important for dimerizationAdd BLAST14

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DRC Bacteria
COG3004 LUCA
HOGENOMiHOG000218489
InParanoidiP13738
KOiK03313
PhylomeDBiP13738

Family and domain databases

Gene3Di1.20.1530.10, 1 hit
HAMAPiMF_01844 NhaA, 1 hit
InterProiView protein in InterPro
IPR023171 Na/H_antiporter_dom_sf
IPR004670 NhaA
PANTHERiPTHR30341 PTHR30341, 1 hit
PfamiView protein in Pfam
PF06965 Na_H_antiport_1, 1 hit
TIGRFAMsiTIGR00773 NhaA, 1 hit

Sequencei

Sequence statusi: Complete.

P13738-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV
60 70 80 90 100
GSLEINKNML LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA
110 120 130 140 150
AIGGMIVPAL LYLAFNYADP ITREGWAIPA ATDIAFALGV LALLGSRVPL
160 170 180 190 200
ALKIFLMALA IIDDLGAIII IALFYTNDLS MASLGVAAVA IAVLAVLNLC
210 220 230 240 250
GARRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK EKHGRSPAKR
260 270 280 290 300
LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK
310 320 330 340 350
PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF
360 370 380
GSVDPELINW AKLGILVGSI SSAVIGYSWL RVRLRPSV
Length:388
Mass (Da):41,356
Last modified:February 1, 1996 - v3
Checksum:iB508B1D2E5EE9130
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03879 Genomic DNA Translation: AAA23448.2
U00096 Genomic DNA Translation: AAC73130.1
AP009048 Genomic DNA Translation: BAB96592.1
S67239 Genomic DNA Translation: AAB20348.1
PIRiC64722
RefSeqiNP_414560.1, NC_000913.3
WP_000681354.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73130; AAC73130; b0019
BAB96592; BAB96592; BAB96592
GeneIDi944758
KEGGiecj:JW0018
eco:b0019
PATRICifig|1411691.4.peg.2265

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03879 Genomic DNA Translation: AAA23448.2
U00096 Genomic DNA Translation: AAC73130.1
AP009048 Genomic DNA Translation: BAB96592.1
S67239 Genomic DNA Translation: AAB20348.1
PIRiC64722
RefSeqiNP_414560.1, NC_000913.3
WP_000681354.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZCDX-ray3.45A/B1-388[»]
3FI1electron microscopy7.00A9-384[»]
4ATVX-ray3.50A/B/C/D1-388[»]
4AU5X-ray3.70A/B/C/D1-388[»]
ProteinModelPortaliP13738
SMRiP13738
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259720, 41 interactors
DIPiDIP-10335N
STRINGi316385.ECDH10B_0020

Chemistry databases

BindingDBiP13738
ChEMBLiCHEMBL5478

Protein family/group databases

TCDBi2.A.33.1.1 the nhaa na(+):h(+) antiporter (nhaa) family

Proteomic databases

PaxDbiP13738
PRIDEiP13738

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73130; AAC73130; b0019
BAB96592; BAB96592; BAB96592
GeneIDi944758
KEGGiecj:JW0018
eco:b0019
PATRICifig|1411691.4.peg.2265

Organism-specific databases

EchoBASEiEB0646
EcoGeneiEG10652 nhaA

Phylogenomic databases

eggNOGiENOG4105DRC Bacteria
COG3004 LUCA
HOGENOMiHOG000218489
InParanoidiP13738
KOiK03313
PhylomeDBiP13738

Enzyme and pathway databases

BioCyciEcoCyc:NHAA-MONOMER
MetaCyc:NHAA-MONOMER

Miscellaneous databases

EvolutionaryTraceiP13738
PROiPR:P13738

Family and domain databases

Gene3Di1.20.1530.10, 1 hit
HAMAPiMF_01844 NhaA, 1 hit
InterProiView protein in InterPro
IPR023171 Na/H_antiporter_dom_sf
IPR004670 NhaA
PANTHERiPTHR30341 PTHR30341, 1 hit
PfamiView protein in Pfam
PF06965 Na_H_antiport_1, 1 hit
TIGRFAMsiTIGR00773 NhaA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNHAA_ECOLI
AccessioniPrimary (citable) accession number: P13738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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