UniProtKB - P13721 (SIAT1_RAT)
Beta-galactoside alpha-2,6-sialyltransferase 1
St6gal1
Functioni
Catalytic activityi
- a β-D-galactoside + CMP-N-acetyl-β-neuraminate = an N-acetyl-α-neuraminyl-(2→6)-β-D-galactosyl derivative + CMP + H+3 PublicationsEC:2.4.99.13 Publications
Kineticsi
- KM=92 µM for donor CMP-Neu5Ac (at pH 6.5 and 37 degrees Celsius)
- KM=1.8 mM for acceptor beta-Gal1,4-GlcNAc (at pH 6.5 and 37 degrees Celsius)
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 186 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 209 | SubstrateBy similarity | 1 | |
Binding sitei | 230 | SubstrateBy similarity | 1 | |
Binding sitei | 350 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 351 | SubstrateBy similarity | 1 | |
Binding sitei | 362 | SubstrateBy similarity | 1 | |
Binding sitei | 366 | SubstrateBy similarity | 1 | |
Binding sitei | 373 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- beta-galactoside alpha-2,6-sialyltransferase activity Source: UniProtKB
- protein homodimerization activity Source: RGD
- sialyltransferase activity Source: RGD
GO - Biological processi
- N-acetylneuraminate metabolic process Source: UniProtKB
- negative regulation of chemotaxis Source: RGD
- negative regulation of macrophage apoptotic process Source: RGD
- positive regulation of mononuclear cell proliferation Source: RGD
- protein glycosylation Source: RGD
- protein N-linked glycosylation via asparagine Source: UniProtKB
- protein sialylation Source: RGD
- regulation of substrate adhesion-dependent cell spreading Source: RGD
- response to ethanol Source: RGD
- sialylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.99.1, 5301 |
Reactomei | R-RNO-4085001, Sialic acid metabolism R-RNO-975577, N-Glycan antennae elongation R-RNO-977068, Termination of O-glycan biosynthesis |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT29, Glycosyltransferase Family 29 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.13 Publications)Short name: Alpha 2,6-ST 1 Alternative name(s): CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1 ST6Gal I Short name: ST6GalI Sialyltransferase 1 |
Gene namesi | Name:St6gal1 Synonyms:Siat1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3676, St6gal1 |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Golgi apparatus
- Golgi stack membrane 1 Publication; Single-pass type II membrane protein Curated
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Golgi apparatus
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi medial cisterna Source: RGD
- Golgi membrane Source: RGD
- Golgi trans cisterna Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | Cytoplasmic | 9 | |
Transmembranei | 10 – 26 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 17 | |
Topological domaini | 27 – 403 | LumenalSequence analysisAdd BLAST | 377 |
Keywords - Cellular componenti
Golgi apparatus, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 119 | Y → A: Protein destabilization. 1 Publication | 1 | |
Mutagenesisi | 119 | Y → F: No effect on protein stability, nor on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 180 | R → A: Small decrease of sialyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 181 | C → A: Loss of sialyltransferase activity and aberrant subcellular location. 1 Publication | 1 | |
Mutagenesisi | 208 | F → A: Reduced KM/Kcat values for CMP-Neu5Ac, no effect on kinetic constants for beta-Gal1,4-GlcNAc. 1 Publication | 1 | |
Mutagenesisi | 230 | N → A: Loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 231 | S → A: Protein destabilization. 1 Publication | 1 | |
Mutagenesisi | 232 | Q → A: Decreased catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 271 | D → A: Decreased catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 272 | Y → A: Strongly decreased catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 319 | S → A: Protein destabilization. 1 Publication | 1 | |
Mutagenesisi | 332 | C → A: Loss of sialyltransferase activity and aberrant subcellular location. 1 Publication | 1 | |
Mutagenesisi | 332 | C → S: Loss of sialyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 333 | D → A: Small decrease of sialyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 350 | C → A: Loss of catalytic activity; when associated with A-361. 1 Publication | 1 | |
Mutagenesisi | 354 | Q → A: No effect on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 355 | K → A: No effect on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 361 | C → A: Loss of catalytic activity; when associated with A-350. 1 Publication | 1 | |
Mutagenesisi | 366 | Y → A: Loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 367 | H → A: Loss of catalytic activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4276 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149251 | 1 – 403 | Beta-galactoside alpha-2,6-sialyltransferase 1Add BLAST | 403 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 139 ↔ 403 | Combined sources1 Publication | ||
Glycosylationi | 146 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 181 ↔ 332 | Combined sources2 Publications | ||
Disulfide bondi | 350 ↔ 361 | By similarity | ||
Modified residuei | 366 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P13721 |
PRIDEi | P13721 |
PTM databases
GlyGeni | P13721, 2 sites |
iPTMneti | P13721 |
PhosphoSitePlusi | P13721 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000001823, Expressed in liver and 22 other tissues |
Interactioni
Subunit structurei
GO - Molecular functioni
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
IntActi | P13721, 1 interactor |
STRINGi | 10116.ENSRNOP00000002499 |
Chemistry databases
BindingDBi | P13721 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 403 | Catalytic (soluble form)1 PublicationAdd BLAST | 341 | |
Regioni | 319 – 321 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2692, Eukaryota |
HOGENOMi | CLU_038334_0_0_1 |
InParanoidi | P13721 |
OMAi | ALHCHLR |
OrthoDBi | 494294at2759 |
PhylomeDBi | P13721 |
TreeFami | TF323961 |
Family and domain databases
Gene3Di | 3.90.1480.20, 1 hit |
InterProi | View protein in InterPro IPR001675, Glyco_trans_29 IPR038578, GT29-like_sf IPR012163, Sialyl_trans |
Pfami | View protein in Pfam PF00777, Glyco_transf_29, 1 hit |
PIRSFi | PIRSF005557, Sialyl_trans, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIHTNLKKKF SLFILVFLLF AVICVWKKGS DYEALTLQAK EFQMPKSQEK
60 70 80 90 100
VAMGSASQVV FSNSKQDPKE DIPILSYHRV TAKVKPQPSF QVWDKDSTYS
110 120 130 140 150
KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV KFSVEALRCH LRDHVNVSMI
160 170 180 190 200
EATDFPFNTT EWEGYLPKEN FRTKVGPWQR CAVVSSAGSL KNSQLGREID
210 220 230 240 250
NHDAVLRFNG APTDNFQQDV GSKTTIRLMN SQLVTTEKRF LKDSLYTEGI
260 270 280 290 300
LIVWDPSVYH ADIPKWYQKP DYNFFETYKS YRRLNPSQPF YILKPQMPWE
310 320 330 340 350
LWDIIQEISA DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC
360 370 380 390 400
YYHQKFFDSA CTMGAYHPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN
IRC
The sequence of this isoform differs from the canonical sequence as follows:
1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGL...LSLLLLVLGK
The sequence of this isoform differs from the canonical sequence as follows:
1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGLPHS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF2Z3S3 | F2Z3S3_RAT | Beta-galactoside alpha-2,6-sialyltr... | St6gal1 | 214 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 367 | H → D in AAA41196 (PubMed:3121604). | 1 | ||
Isoform RKA1 Publication (identifier: P13721-2) | |||||
Sequence conflicti | 40 | V → L in AAB06269 (PubMed:1983783).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001782 | 1 – 232 | MIHTN…LMNSQ → MRYLLFWYGLPHSYSQCVCH WTPASGIFENEPLLSLLLLV LGK in isoform RKA. 1 PublicationAdd BLAST | 232 | |
Alternative sequenceiVSP_001783 | 1 – 232 | MIHTN…LMNSQ → MRYLLFWYGLPHS in isoform RKB. 1 PublicationAdd BLAST | 232 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18769 mRNA Translation: AAA41196.1 M73987 mRNA Translation: AAB06269.1 M83143 mRNA Translation: AAB07233.1 AABR07034602 Genomic DNA No translation available. AABR07034601 Genomic DNA No translation available. AABR07034600 Genomic DNA No translation available. CH473999 Genomic DNA Translation: EDL78084.1 CH473999 Genomic DNA Translation: EDL78085.1 |
PIRi | A28451 C42327 |
RefSeqi | NP_001106815.1, NM_001113344.1 [P13721-1] NP_671738.2, NM_147205.2 XP_006248563.1, XM_006248501.1 [P13721-1] XP_008767014.1, XM_008768792.2 [P13721-3] |
Genome annotation databases
GeneIDi | 25197 |
KEGGi | rno:25197 |
UCSCi | RGD:3676, rat [P13721-1] |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18769 mRNA Translation: AAA41196.1 M73987 mRNA Translation: AAB06269.1 M83143 mRNA Translation: AAB07233.1 AABR07034602 Genomic DNA No translation available. AABR07034601 Genomic DNA No translation available. AABR07034600 Genomic DNA No translation available. CH473999 Genomic DNA Translation: EDL78084.1 CH473999 Genomic DNA Translation: EDL78085.1 |
PIRi | A28451 C42327 |
RefSeqi | NP_001106815.1, NM_001113344.1 [P13721-1] NP_671738.2, NM_147205.2 XP_006248563.1, XM_006248501.1 [P13721-1] XP_008767014.1, XM_008768792.2 [P13721-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4MPS | X-ray | 2.40 | A/B | 95-403 | [»] | |
SMRi | P13721 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P13721, 1 interactor |
STRINGi | 10116.ENSRNOP00000002499 |
Chemistry databases
BindingDBi | P13721 |
ChEMBLi | CHEMBL4276 |
Protein family/group databases
CAZyi | GT29, Glycosyltransferase Family 29 |
PTM databases
GlyGeni | P13721, 2 sites |
iPTMneti | P13721 |
PhosphoSitePlusi | P13721 |
Proteomic databases
PaxDbi | P13721 |
PRIDEi | P13721 |
Genome annotation databases
GeneIDi | 25197 |
KEGGi | rno:25197 |
UCSCi | RGD:3676, rat [P13721-1] |
Organism-specific databases
CTDi | 6480 |
RGDi | 3676, St6gal1 |
Phylogenomic databases
eggNOGi | KOG2692, Eukaryota |
HOGENOMi | CLU_038334_0_0_1 |
InParanoidi | P13721 |
OMAi | ALHCHLR |
OrthoDBi | 494294at2759 |
PhylomeDBi | P13721 |
TreeFami | TF323961 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.99.1, 5301 |
Reactomei | R-RNO-4085001, Sialic acid metabolism R-RNO-975577, N-Glycan antennae elongation R-RNO-977068, Termination of O-glycan biosynthesis |
Miscellaneous databases
PROi | PR:P13721 |
Gene expression databases
Bgeei | ENSRNOG00000001823, Expressed in liver and 22 other tissues |
Family and domain databases
Gene3Di | 3.90.1480.20, 1 hit |
InterProi | View protein in InterPro IPR001675, Glyco_trans_29 IPR038578, GT29-like_sf IPR012163, Sialyl_trans |
Pfami | View protein in Pfam PF00777, Glyco_transf_29, 1 hit |
PIRSFi | PIRSF005557, Sialyl_trans, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SIAT1_RAT | |
Accessioni | P13721Primary (citable) accession number: P13721 Secondary accession number(s): D4ABR2, G3V680 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | December 2, 2020 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families