UniProtKB - P13717 (NUCA_SERMA)
Protein
Nuclease
Gene
nucA
Organism
Serratia marcescens
Status
Functioni
Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.
Miscellaneous
The active site contains 1 hydrated magnesium ion that has only 1 direct interaction with the protein; all other interactions are via water molecules.
Catalytic activityi
- Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.PROSITE-ProRule annotation EC:3.1.30.2
Cofactori
Mg2+Note: Binds 1 Mg2+ ion.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 110 | Proton acceptorPROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 140 | Magnesium; catalytic | 1 |
GO - Molecular functioni
- endonuclease activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- nucleic acid binding Source: InterPro
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.30.2, 5690 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclease (EC:3.1.30.2)Alternative name(s): Endonuclease Cleaved into the following 3 chains: |
Gene namesi | Name:nucA Synonyms:nuc |
Organismi | Serratia marcescens |
Taxonomic identifieri | 615 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Yersiniaceae › Serratia |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | R → A: Reduced activity 1000-fold. 1 Publication | 1 | |
Mutagenesisi | 78 | R → K: Reduced activity 100-fold. 1 Publication | 1 | |
Mutagenesisi | 108 | R → A: Reduced activity 1000-fold. 1 Publication | 1 | |
Mutagenesisi | 108 | R → K: Reduced activity 50-fold. 1 Publication | 1 | |
Mutagenesisi | 110 | H → A, D, E, K or Q: Reduced activity 100000-fold. 1 Publication | 1 | |
Mutagenesisi | 110 | H → N: Reduced activity 1000-fold. 1 Publication | 1 | |
Mutagenesisi | 140 | N → A, D or H: Reduced activity over 10000-fold. 1 Publication | 1 | |
Mutagenesisi | 140 | N → Q: Reduced activity 1000-fold. 1 Publication | 1 | |
Mutagenesisi | 148 | E → A, D or Q: Reduced activity over 800-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Add BLAST | 21 | |
ChainiPRO_0000019914 | 22 – 266 | Nuclease isoform Sm2Add BLAST | 245 | |
ChainiPRO_0000019915 | 23 – 266 | Nuclease isoform Sm3Add BLAST | 244 | |
ChainiPRO_0000019916 | 25 – 266 | Nuclease isoform Sm1Add BLAST | 242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 34 | |||
Disulfide bondi | 222 ↔ 264 |
Keywords - PTMi
Disulfide bondProteomic databases
PRIDEi | P13717 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P13717 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13717 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DNA/RNA non-specific endonuclease family.Curated
Keywords - Domaini
SignalPhylogenomic databases
OrthoDBi | 1438016at2 |
Family and domain databases
InterProi | View protein in InterPro IPR018524, DNA/RNA_endonuclease_AS IPR001604, DNA/RNA_non-sp_Endonuclease IPR020821, Extracellular_endonuc_su_A IPR040255, Non-specific_endonuclease |
PANTHERi | PTHR13966, PTHR13966, 1 hit |
Pfami | View protein in Pfam PF01223, Endonuclease_NS, 1 hit |
SMARTi | View protein in SMART SM00892, Endonuclease_NS, 1 hit SM00477, NUC, 1 hit |
PROSITEi | View protein in PROSITE PS01070, NUCLEASE_NON_SPEC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P13717-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFNNKMLAL AALLFAAQAS ADTLESIDNC AVGCPTGGSS NVSIVRHAYT
60 70 80 90 100
LNNNSTTKFA NWVAYHITKD TPASGKTRNW KTDPALNPAD TLAPADYTGA
110 120 130 140 150
NAALKVDRGH QAPLASLAGV SDWESLNYLS NITPQKSDLN QGAWARLEDQ
160 170 180 190 200
ERKLIDRADI SSVYTVTGPL YERDMGKLPG TQKAHTIPSA YWKVIFINNS
210 220 230 240 250
PAVNHYAAFL FDQNTPKGAD FCQFRVTVDE IEKRTGLIIW AGLPDDVQAS
260
LKSKPGVLPE LMGCKN
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19495 Genomic DNA Translation: AAA26560.1 |
RefSeqi | WP_015377376.1, NZ_WVHX01000034.1 |
Genome annotation databases
GeneIDi | 57760601 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19495 Genomic DNA Translation: AAA26560.1 |
RefSeqi | WP_015377376.1, NZ_WVHX01000034.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G8T | X-ray | 1.10 | A/B | 22-266 | [»] | |
1QAE | X-ray | 2.05 | A/B | 22-266 | [»] | |
1QL0 | X-ray | 1.10 | A/B | 26-266 | [»] | |
1SMN | X-ray | 2.04 | A/B | 22-266 | [»] | |
4E3Y | X-ray | 0.95 | A/B | 22-266 | [»] | |
SMRi | P13717 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P13717 |
Genome annotation databases
GeneIDi | 57760601 |
Phylogenomic databases
OrthoDBi | 1438016at2 |
Enzyme and pathway databases
BRENDAi | 3.1.30.2, 5690 |
Miscellaneous databases
EvolutionaryTracei | P13717 |
Family and domain databases
InterProi | View protein in InterPro IPR018524, DNA/RNA_endonuclease_AS IPR001604, DNA/RNA_non-sp_Endonuclease IPR020821, Extracellular_endonuc_su_A IPR040255, Non-specific_endonuclease |
PANTHERi | PTHR13966, PTHR13966, 1 hit |
Pfami | View protein in Pfam PF01223, Endonuclease_NS, 1 hit |
SMARTi | View protein in SMART SM00892, Endonuclease_NS, 1 hit SM00477, NUC, 1 hit |
PROSITEi | View protein in PROSITE PS01070, NUCLEASE_NON_SPEC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUCA_SERMA | |
Accessioni | P13717Primary (citable) accession number: P13717 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | February 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families