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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADUniRule annotationCombined sources1 Publication1
Binding sitei42NADUniRule annotation1
Binding sitei68NADUniRule annotation1
Binding sitei85SubstrateUniRule annotation1
Binding sitei91SubstrateUniRule annotation1
Binding sitei146NADUniRule annotation1
Binding sitei156Allosteric activatorUniRule annotationCombined sources1 Publication1
Active sitei178Proton acceptorUniRule annotationCombined sources1 Publication1
Binding sitei232SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 16NADCombined sources1 Publication2
Nucleotide bindingi82 – 83NADUniRule annotationCombined sources1 Publication2
Nucleotide bindingi121 – 123NADUniRule annotationCombined sources1 Publication3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciBSUB:BSU03050-MONOMER
SABIO-RKiP13714
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDH1 PublicationUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Synonyms:lctE1 Publication
Ordered Locus Names:BSU03050
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683291 – 320L-lactate dehydrogenaseAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineUniRule annotation1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13714
PRIDEiP13714

PTM databases

iPTMnetiP13714

Expressioni

Inductioni

Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM.1 Publication

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001683

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi15 – 27Combined sources13
Beta strandi31 – 36Combined sources6
Helixi40 – 52Combined sources13
Helixi54 – 56Combined sources3
Beta strandi57 – 59Combined sources3
Beta strandi62 – 66Combined sources5
Helixi68 – 71Combined sources4
Beta strandi75 – 79Combined sources5
Helixi91 – 111Combined sources21
Beta strandi116 – 120Combined sources5
Beta strandi122 – 124Combined sources3
Helixi125 – 136Combined sources12
Helixi140 – 142Combined sources3
Beta strandi143 – 145Combined sources3
Helixi149 – 160Combined sources12
Turni161 – 164Combined sources4
Helixi167 – 169Combined sources3
Beta strandi174 – 179Combined sources6
Helixi186 – 188Combined sources3
Helixi196 – 201Combined sources6
Beta strandi204 – 206Combined sources3
Helixi208 – 229Combined sources22
Helixi234 – 248Combined sources15
Beta strandi253 – 255Combined sources3
Beta strandi258 – 263Combined sources6
Helixi264 – 266Combined sources3
Beta strandi268 – 273Combined sources6
Beta strandi276 – 279Combined sources4
Beta strandi282 – 286Combined sources5
Helixi293 – 310Combined sources18
Turni311 – 313Combined sources3

3D structure databases

ProteinModelPortaliP13714
SMRiP13714
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 126Substrate bindingUniRule annotation4
Regioni151 – 154Substrate bindingUniRule annotation4
Regioni168 – 172Allosteric activator bindingCombined sources1 Publication5

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
InParanoidiP13714
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

P13714-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKHVNKVAL IGAGFVGSSY AFALINQGIT DELVVIDVNK EKAMGDVMDL
60 70 80 90 100
NHGKAFAPQP VKTSYGTYED CKDADIVCIC AGANQKPGET RLELVEKNLK
110 120 130 140 150
IFKGIVSEVM ASGFDGIFLV ATNPVDILTY ATWKFSGLPK ERVIGSGTTL
160 170 180 190 200
DSARFRFMLS EYFGAAPQNV HAHIIGEHGD TELPVWSHAN VGGVPVSELV
210 220 230 240 250
EKNDAYKQEE LDQIVDDVKN AAYHIIEKKG ATYYGVAMSL ARITKAILHN
260 270 280 290 300
ENSILTVSTY LDGQYGADDV YIGVPAVVNR GGIAGITELN LNEKEKEQFL
310 320
HSAGVLKNIL KPHFAEQKVN
Length:320
Mass (Da):34,802
Last modified:May 26, 2009 - v3
Checksum:iF0B71F5A760FCAE1
GO

Sequence cautioni

The sequence BAA08939 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB12099 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38V → L AA sequence (PubMed:3098260).Curated1
Sequence conflicti51N → P in BAA08939 (PubMed:8969502).Curated1
Sequence conflicti57 – 58AP → GL in BAA08939 (PubMed:8969502).Curated2
Sequence conflicti120V → I AA sequence (PubMed:3098260).Curated1
Sequence conflicti224H → T AA sequence (PubMed:3098260).Curated1
Sequence conflicti315 – 318Missing AA sequence (PubMed:3098260).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50453 Genomic DNA Translation: BAA08939.1 Different initiation.
AL009126 Genomic DNA Translation: CAB12099.2 Different initiation.
PIRiE69649
RefSeqiNP_388187.2, NC_000964.3

Genome annotation databases

EnsemblBacteriaiCAB12099; CAB12099; BSU03050
GeneIDi938348
KEGGibsu:BSU03050
PATRICifig|224308.43.peg.313

Similar proteinsi

Entry informationi

Entry nameiLDH_BACSU
AccessioniPrimary (citable) accession number: P13714
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 26, 2009
Last modified: May 23, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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