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Protein

DNA mismatch repair protein Msh3

Gene

Msh3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi850 – 857ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: GO_Central
  • centromeric DNA binding Source: MGI
  • damaged DNA binding Source: MGI
  • DNA binding Source: MGI
  • DNA-dependent ATPase activity Source: GO_Central
  • double-strand/single-strand DNA junction binding Source: GO_Central
  • enzyme binding Source: MGI
  • heteroduplex DNA loop binding Source: GO_Central
  • oxidized purine DNA binding Source: GO_Central
  • Y-form DNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein Msh3
Alternative name(s):
Protein repair-1
Short name:
REP-1
Protein repair-3
Short name:
REP-3
Gene namesi
Name:Msh3
Synonyms:Rep-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109519 Msh3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001151931 – 1091DNA mismatch repair protein Msh3Add BLAST1091

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP13705
MaxQBiP13705
PaxDbiP13705
PeptideAtlasiP13705
PRIDEiP13705

PTM databases

iPTMnetiP13705
PhosphoSitePlusiP13705

Expressioni

Gene expression databases

CleanExiMM_MSH3

Interactioni

Subunit structurei

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Interacts with MCM9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201526, 1 interactor
ComplexPortaliCPX-78 DNA mismatch repair MutSbeta complex
STRINGi10090.ENSMUSP00000022220

Structurei

3D structure databases

ProteinModelPortaliP13705
SMRiP13705
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0217 Eukaryota
COG0249 LUCA
HOVERGENiHBG006400
InParanoidiP13705
KOiK08736
PhylomeDBiP13705

Family and domain databases

Gene3Di3.30.420.110, 1 hit
3.40.1170.10, 1 hit
InterProiView protein in InterPro
IPR007695 DNA_mismatch_repair_MutS-lik_N
IPR017261 DNA_mismatch_repair_MutS/MSH
IPR000432 DNA_mismatch_repair_MutS_C
IPR007696 DNA_mismatch_repair_MutS_core
IPR016151 DNA_mismatch_repair_MutS_N
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01624 MutS_I, 1 hit
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF037677 DNA_mis_repair_Msh6, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
SSF53150 SSF53150, 1 hit
SSF55271 SSF55271, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

P13705-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPRGKSASGG STAAGPGPGR QTVLSRFFRS AGSLRSSASS TEPAEKVTEG
60 70 80 90 100
DSRKRSLGNG GPTKKKARKV PEKEEENISV ASHHPEAKKC LRPRIVLKSL
110 120 130 140 150
EKLKEFCCDS ALPQNRVQTE ALRERLEVLP RCTDFEDITL QRAKNAVLSE
160 170 180 190 200
DSKSQANQKD SQFGPCPEVF QKTSDCKPFN KRSKSVYTPL ELQYLDMKQQ
210 220 230 240 250
HKDAVLCVEC GYKYRFFGED AEIAARELNI YCHLDHNFMT ASIPTHRLFV
260 270 280 290 300
HVRRLVAKGY KVGVVKQTET AALKAIGDNK SSVFSRKLTA LYTKSTLIGE
310 320 330 340 350
DVNPLIRLDD SVNIDEVMTD TSTNYLLCIY EEKENIKDKK KGNLSVGVVG
360 370 380 390 400
VQPATGEVVF DCFQDSASRL ELETRISSLQ PVELLLPSDL SEPTEMLIQR
410 420 430 440 450
ATNVSVRDDR IRVERMNNTY FEYSHAFQTV TEFYAREIVD SQGSQSLSGV
460 470 480 490 500
INLEKPVICA LAAVIRYLKE FNLEKMLSKP ESFKQLSSGM EFMRINGTTL
510 520 530 540 550
RNLEMVQNQT DMKTKGSLLW VLDHTKTSFG RRKLKNWVTQ PLLKLREINA
560 570 580 590 600
RLDAVSDVLH SESSVFEQIE NLLRKLPDVE RGLCSIYHKK CSTQEFFLIV
610 620 630 640 650
KSLCQLKSEL QALMPAVNSH VQSDLLRALI VELLSPVEHY LKVLNGPAAK
660 670 680 690 700
VGDKTELFKD LSDFPLIKKR KNEIQEVIHS IQMRLQEFRK ILKLPSLQYV
710 720 730 740 750
TVSGQEFMIE IKNSAVSCIP ADWVKVGSTK AVSRFHPPFI VESYRRLNQL
760 770 780 790 800
REQLVLDCNA EWLGFLENFG EHYHTLCKAV DHLATVDCIF SLAKVAKQGN
810 820 830 840 850
YCRPTLQEEK KIIIKNGRHP MIDVLLGEQD QFVPNSTSLS DSERVMIITG
860 870 880 890 900
PNMGGKSSYI KQVTLVTIMA QIGSYVPAEE ATIGIVDGIF TRMGAADNIY
910 920 930 940 950
KGRSTFMEQL TDTAEIIRRA SPQSLVILDE LGRGTSTHDG IAIAYATLEY
960 970 980 990 1000
FIRDVKSLTL FVTHYPPVCE LEKCYPEQVG NYHMGFLVNE DESKQDSGDM
1010 1020 1030 1040 1050
EQMPDSVTFL YQITRGIAAR SYGLNVAKLA DVPREVLQKA AHKSKELEGL
1060 1070 1080 1090
VSLRRKRLEC FTDLWMTHSV KDLHTWADKL EMEEIQTSLP H
Length:1,091
Mass (Da):123,074
Last modified:July 1, 1993 - v3
Checksum:i822BF422436FD513
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WQ16A0A087WQ16_MOUSE
DNA mismatch repair protein Msh3
Msh3
1,095Annotation score:
A0A087WSS2A0A087WSS2_MOUSE
DNA mismatch repair protein Msh3
Msh3
360Annotation score:
E9QPY6E9QPY6_MOUSE
DNA mismatch repair protein Msh3
Msh3
1,094Annotation score:
A0A087WQZ2A0A087WQZ2_MOUSE
DNA mismatch repair protein Msh3
Msh3
129Annotation score:
A0A087WP43A0A087WP43_MOUSE
DNA mismatch repair protein Msh3
Msh3
101Annotation score:
A0A087WRJ5A0A087WRJ5_MOUSE
DNA mismatch repair protein Msh3
Msh3
159Annotation score:
A0A087WP45A0A087WP45_MOUSE
DNA mismatch repair protein Msh3
Msh3
167Annotation score:
A0A087WSR8A0A087WSR8_MOUSE
DNA mismatch repair protein Msh3
Msh3
56Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti505 – 506MV → IL in AAB60711 (PubMed:7926796).Curated2
Sequence conflicti864T → A in AAB60711 (PubMed:7926796).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80360 mRNA Translation: AAA40052.1
L10319
, L10295, L10296, L10297, L10298, L10299, L10300, L10301, L10304, L10305, L10306, L10307, L10308, L10309, L10310, L10311, L10312, L10313, L10314, L10315, L10316, L10317, L10318 Genomic DNA Translation: AAB60711.1
M24919 mRNA Translation: AAA40051.1 Sequence problems.
PIRiA32495
RefSeqiNP_034959.2, NM_010829.2
UniGeneiMm.343101

Genome annotation databases

GeneIDi17686
KEGGimmu:17686

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80360 mRNA Translation: AAA40052.1
L10319
, L10295, L10296, L10297, L10298, L10299, L10300, L10301, L10304, L10305, L10306, L10307, L10308, L10309, L10310, L10311, L10312, L10313, L10314, L10315, L10316, L10317, L10318 Genomic DNA Translation: AAB60711.1
M24919 mRNA Translation: AAA40051.1 Sequence problems.
PIRiA32495
RefSeqiNP_034959.2, NM_010829.2
UniGeneiMm.343101

3D structure databases

ProteinModelPortaliP13705
SMRiP13705
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201526, 1 interactor
ComplexPortaliCPX-78 DNA mismatch repair MutSbeta complex
STRINGi10090.ENSMUSP00000022220

PTM databases

iPTMnetiP13705
PhosphoSitePlusiP13705

Proteomic databases

EPDiP13705
MaxQBiP13705
PaxDbiP13705
PeptideAtlasiP13705
PRIDEiP13705

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17686
KEGGimmu:17686

Organism-specific databases

CTDi4437
MGIiMGI:109519 Msh3

Phylogenomic databases

eggNOGiKOG0217 Eukaryota
COG0249 LUCA
HOVERGENiHBG006400
InParanoidiP13705
KOiK08736
PhylomeDBiP13705

Miscellaneous databases

ChiTaRSiMsh3 mouse
PROiPR:P13705
SOURCEiSearch...

Gene expression databases

CleanExiMM_MSH3

Family and domain databases

Gene3Di3.30.420.110, 1 hit
3.40.1170.10, 1 hit
InterProiView protein in InterPro
IPR007695 DNA_mismatch_repair_MutS-lik_N
IPR017261 DNA_mismatch_repair_MutS/MSH
IPR000432 DNA_mismatch_repair_MutS_C
IPR007696 DNA_mismatch_repair_MutS_core
IPR016151 DNA_mismatch_repair_MutS_N
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01624 MutS_I, 1 hit
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF037677 DNA_mis_repair_Msh6, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
SSF53150 SSF53150, 1 hit
SSF55271 SSF55271, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMSH3_MOUSE
AccessioniPrimary (citable) accession number: P13705
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 1, 1993
Last modified: November 7, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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