Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartate-semialdehyde dehydrogenase

Gene

HOM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.By similarity

Miscellaneous

Present with 18400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
  4. Homoserine kinase (THR1)
  5. Threonine synthase (THR4)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112PhosphateBy similarity1
Active sitei156Acyl-thioester intermediateBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei211SubstrateBy similarity1
Binding sitei214PhosphateBy similarity1
Binding sitei249SubstrateBy similarity1
Active sitei256Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 16NADPBy similarity4
Nucleotide bindingi40 – 41NADPBy similarity2
Nucleotide bindingi187 – 188NADPBy similarity2
Nucleotide bindingi343 – 344NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • homoserine biosynthetic process Source: SGD
  • isoleucine biosynthetic process Source: InterPro
  • lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
  • methionine biosynthetic process Source: SGD
  • threonine biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Lysine biosynthesis, Methionine biosynthesis, Threonine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciYEAST:YDR158W-MONOMER
UniPathwayiUPA00034; UER00016
UPA00050; UER00463
UPA00051; UER00464

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate-semialdehyde dehydrogenase (EC:1.2.1.11)
Short name:
ASA dehydrogenase
Short name:
ASADH
Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenase
Gene namesi
Name:HOM2
Ordered Locus Names:YDR158W
ORF Names:YD8358.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR158W
SGDiS000002565 HOM2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001414001 – 365Aspartate-semialdehyde dehydrogenaseAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphothreonineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei323PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13663
PaxDbiP13663
PRIDEiP13663

2D gel databases

SWISS-2DPAGEiP13663

PTM databases

iPTMnetiP13663

Expressioni

Inductioni

By high concentrations of Met (general AA biosynthesis control).

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32209, 339 interactors
DIPiDIP-5204N
IntActiP13663, 23 interactors
STRINGi4932.YDR158W

Structurei

3D structure databases

ProteinModelPortaliP13663
SMRiP13663
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000013358
InParanoidiP13663
KOiK00133
OMAiCEEEMKM
OrthoDBiEOG092C309U

Family and domain databases

HAMAPiMF_02121 ASADH, 1 hit
InterProiView protein in InterPro
IPR000319 Asp-semialdehyde_DH_CS
IPR005676 Asp_semi-ald_DH_pep-lack
IPR012080 Asp_semialdehyde_DH
IPR036291 NAD(P)-bd_dom_sf
IPR000534 Semialdehyde_DH_NAD-bd
IPR012280 Semialdhyde_DH_dimer_dom
PfamiView protein in Pfam
PF01118 Semialdhyde_dh, 1 hit
PF02774 Semialdhyde_dhC, 1 hit
SMARTiView protein in SMART
SM00859 Semialdhyde_dh, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00978 asd_EA, 1 hit
PROSITEiView protein in PROSITE
PS01103 ASD, 1 hit

Sequencei

Sequence statusi: Complete.

P13663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKKIAGVL GATGSVGQRF ILLLANHPHF ELKVLGASSR SAGKKYVDAV
60 70 80 90 100
NWKQTDLLPE SATDIIVSEC KSEFFKECDI VFSGLDADYA GAIEKEFMEA
110 120 130 140 150
GIAIVSNAKN YRREQDVPLI VPVVNPEHLD IVAQKLDTAK AQGKPRPGFI
160 170 180 190 200
ICISNCSTAG LVAPLKPLIE KFGPIDALTT TTLQAISGAG FSPGVPGIDI
210 220 230 240 250
LDNIIPYIGG EEDKMEWETK KILAPLAEDK THVKLLTPEE IKVSAQCNRV
260 270 280 290 300
AVSDGHTECI SLRFKNRPAP SVEQVKTCLK EYVCDAYKLG CHSAPKQTIH
310 320 330 340 350
VLEQPDRPQP RLDRNRDSGY GVSVGRIRED PLLDFKMVVL SHNTIIGAAG
360
SGVLIAEILL ARNLI
Length:365
Mass (Da):39,544
Last modified:January 1, 1990 - v1
Checksum:iD33096DA8C2B6424
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15649 Genomic DNA Translation: CAA33675.1
Z50046 Genomic DNA Translation: CAA90378.1
AY557698 Genomic DNA Translation: AAS56024.1
BK006938 Genomic DNA Translation: DAA11998.1
PIRiJQ0198
RefSeqiNP_010442.3, NM_001180465.3

Genome annotation databases

EnsemblFungiiYDR158W; YDR158W; YDR158W
GeneIDi851736
KEGGisce:YDR158W

Similar proteinsi

Entry informationi

Entry nameiDHAS_YEAST
AccessioniPrimary (citable) accession number: P13663
Secondary accession number(s): D6VSD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 18, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health