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UniProtKB - P13656 (CHIA_ECOLI)
Protein
Probable bifunctional chitinase/lysozyme
Gene
chiA
Organism
Escherichia coli (strain K12)
Status
Functioni
Bifunctional enzyme with lysozyme/chitinase activity.
1 PublicationCatalytic activityi
- Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication EC:3.2.1.14
- Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication EC:3.2.1.17
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 700 | Proton donorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- carbohydrate binding Source: InterPro
- chitin binding Source: UniProtKB-KW
- endochitinase activity Source: EcoCyc
- lysozyme activity Source: UniProtKB-EC
GO - Biological processi
- chitin catabolic process Source: UniProtKB-KW
- polysaccharide catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase, Multifunctional enzyme |
Biological process | Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation |
Ligand | Chitin-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11237-MONOMER |
Protein family/group databases
CAZyi | CBM5, Carbohydrate-Binding Module Family 5 GH18, Glycoside Hydrolase Family 18 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:chiA Synonyms:yheB Ordered Locus Names:b3338, JW3300 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Note: Secreted via the Gsp type II secretion machinery under conditions of derepressed gsp gene expression.
Extracellular region or secreted
- extracellular region Source: InterPro
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
- periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000011907 | 25 – 897 | Probable bifunctional chitinase/lysozymeAdd BLAST | 873 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 628 ↔ 673 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P13656 |
PRIDEi | P13656 |
Expressioni
Inductioni
Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261016, 167 interactors |
DIPi | DIP-9276N |
IntActi | P13656, 2 interactors |
STRINGi | 511145.b3338 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 91 | Chitin-binding type-3 1Add BLAST | 67 | |
Domaini | 128 – 194 | Chitin-binding type-3 2Add BLAST | 67 | |
Domaini | 229 – 295 | Chitin-binding type-3 3Add BLAST | 67 | |
Domaini | 337 – 403 | Chitin-binding type-3 4Add BLAST | 67 | |
Domaini | 459 – 529 | Chitin-binding type-3 5Add BLAST | 71 | |
Domaini | 586 – 877 | GH18PROSITE-ProRule annotationAdd BLAST | 292 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 90 – 127 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 182 – 222 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 287 – 333 | DisorderedSequence analysisAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 182 – 199 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 200 – 214 | Pro residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | COG3979, Bacteria |
HOGENOMi | CLU_019399_2_0_6 |
InParanoidi | P13656 |
OMAi | CATSAID |
Family and domain databases
InterProi | View protein in InterPro IPR003610, CBM_fam5/12 IPR036573, CBM_sf_5/12 IPR001223, Glyco_hydro18_cat IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF02839, CBM_5_12, 1 hit |
SMARTi | View protein in SMART SM00495, ChtBD3, 7 hits |
SUPFAMi | SSF51055, SSF51055, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS51910, GH18_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P13656-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLNIFTKSM IGMGLVCSAL PALAMEAWNN QQGGNKYQVI FDGKIYENAW
60 70 80 90 100
WVSSTNCPGK AKANDATNPW RLKRTATAAE ISQFGNTLSC EKSGSSSSSN
110 120 130 140 150
SNTPASNTPA NGGSATPAQG TVPSNSSVVA WNKQQGGQTW YVVFNGAVYK
160 170 180 190 200
NAWWVASSNC PGDAKSNDAS NPWRYVRAAT ATEISETSNP QSCTSAPQPS
210 220 230 240 250
PDVKPAPDVK PAPDVQPAPA DKSNDNYAVV AWKGQEGSST WYVIYNGGIY
260 270 280 290 300
KNAWWVGAAN CPGDAKENDA SNPWRYVRAA TATEISQYGN PGSCSVKPDN
310 320 330 340 350
NGGAVTPVDP TPETPVTPTP DNSEPSTPAD SVNDYSLQAW SGQEGSEIYH
360 370 380 390 400
VIFNGNVYKN AWWVGSKDCP RGTSAENSNN PWRLERTATA AELSQYGNPT
410 420 430 440 450
TCEIDNGGVI VADGFQASKA YSADSIVDYN DAHYKTSVDQ DAWGFVPGGD
460 470 480 490 500
NPWKKYEPAK AWSASTVYVK GDRVVVDGQA YEALFWTQSD NPALVANQNA
510 520 530 540 550
TGSNSRPWKP LGKAQSYSNE ELNNAPQFNP ETLYASDTLI RFNGVNYISQ
560 570 580 590 600
SKVQKVSPSD SNPWRVFVDW TGTKERVGTP KKAWPKHVYA PYVDFTLNTI
610 620 630 640 650
PDLAALAKNH NVNHFTLAFV VSKDANTCLP TWGTAYGMQN YAQYSKIKAL
660 670 680 690 700
REAGGDVMLS IGGANNAPLA ASCKNVDDLM QHYYDIVDNL NLKVLDFDIE
710 720 730 740 750
GTWVADQASI ERRNLAVKKV QDKWKSEGKD IAIWYTLPIL PTGLTPEGMN
760 770 780 790 800
VLSDAKAKGV ELAGVNVMTM DYGNAICQSA NTEGQNIHGK CATSAIANLH
810 820 830 840 850
SQLKGLHPNK SDAEIDAMMG TTPMVGVNDV QGEVFYLSDA RLVMQDAQKR
860 870 880 890
NLGMVGIWSI ARDLPGGTNL SPEFHGLTKE QAPKYAFSEI FAPFTKQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 874 | F → I in AAC13985 (PubMed:2661540).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58135.1 U00096 Genomic DNA Translation: AAC76363.1 AP009048 Genomic DNA Translation: BAE77953.1 M27176 mRNA Translation: AAC13985.1 |
PIRi | E65127 |
RefSeqi | NP_417797.1, NC_000913.3 WP_000773156.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76363; AAC76363; b3338 BAE77953; BAE77953; BAE77953 |
GeneIDi | 947837 |
KEGGi | ecj:JW3300 eco:b3338 |
PATRICi | fig|1411691.4.peg.3393 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58135.1 U00096 Genomic DNA Translation: AAC76363.1 AP009048 Genomic DNA Translation: BAE77953.1 M27176 mRNA Translation: AAC13985.1 |
PIRi | E65127 |
RefSeqi | NP_417797.1, NC_000913.3 WP_000773156.1, NZ_LN832404.1 |
3D structure databases
AlphaFoldDBi | P13656 |
SMRi | P13656 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261016, 167 interactors |
DIPi | DIP-9276N |
IntActi | P13656, 2 interactors |
STRINGi | 511145.b3338 |
Protein family/group databases
CAZyi | CBM5, Carbohydrate-Binding Module Family 5 GH18, Glycoside Hydrolase Family 18 |
Proteomic databases
PaxDbi | P13656 |
PRIDEi | P13656 |
Genome annotation databases
EnsemblBacteriai | AAC76363; AAC76363; b3338 BAE77953; BAE77953; BAE77953 |
GeneIDi | 947837 |
KEGGi | ecj:JW3300 eco:b3338 |
PATRICi | fig|1411691.4.peg.3393 |
Organism-specific databases
EchoBASEi | EB1219 |
Phylogenomic databases
eggNOGi | COG3979, Bacteria |
HOGENOMi | CLU_019399_2_0_6 |
InParanoidi | P13656 |
OMAi | CATSAID |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11237-MONOMER |
Miscellaneous databases
PROi | PR:P13656 |
Family and domain databases
InterProi | View protein in InterPro IPR003610, CBM_fam5/12 IPR036573, CBM_sf_5/12 IPR001223, Glyco_hydro18_cat IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF02839, CBM_5_12, 1 hit |
SMARTi | View protein in SMART SM00495, ChtBD3, 7 hits |
SUPFAMi | SSF51055, SSF51055, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS51910, GH18_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHIA_ECOLI | |
Accessioni | P13656Primary (citable) accession number: P13656 Secondary accession number(s): Q2M703 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families