chiA

Functionⁱ

Bifunctional enzyme with lysozyme/chitinase activity.1 Publication

Catalytic activityⁱ

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	700	Proton donorBy similarity		1

GO - Molecular functionⁱ

carbohydrate binding Source: InterPro
chitin binding Source: UniProtKB-KW
endochitinase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 10760150
lysozyme activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

chitin catabolic process Source: UniProtKB-KW
polysaccharide catabolic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase, Multifunctional enzyme
Biological process	Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation
Ligand	Chitin-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11237-MONOMER MetaCyc:EG11237-MONOMER

BioCycⁱ

EcoCyc:EG11237-MONOMER
MetaCyc:EG11237-MONOMER

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM5 Carbohydrate-Binding Module Family 5 GH18 Glycoside Hydrolase Family 18

CAZyⁱ

CBM5 Carbohydrate-Binding Module Family 5
GH18 Glycoside Hydrolase Family 18

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable bifunctional chitinase/lysozyme Including the following 2 domains: Chitinase (EC:3.2.1.14) Lysozyme (EC:3.2.1.17)
Gene namesⁱ	Name:chiA Synonyms:yheB Ordered Locus Names:b3338, JW3300
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11237 chiA

EcoGeneⁱ

EG11237 chiA

Subcellular locationⁱ

Periplasm
1 Publication
Manual assertion based on experiment inⁱ
- Ref.5
  "The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS."
  Francetic O., Badaut C., Rimsky S., Pugsley A.P.
  Mol. Microbiol. 35:1506-1517(2000) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TRANSCRIPTIONAL REGULATION, GENE NAME.

Note:

Secreted via the Gsp type II secretion machinery under conditions of derepressed gsp gene expression.

GO - Cellular componentⁱ

extracellular region Source: InterPro
outer membrane-bounded periplasmic space
Source: EcoCyc
Inferred from direct assayⁱ
- 10760150
- 24140104
periplasmic space
Source: EcoCyc
Inferred from direct assayⁱ
- 10760150

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Periplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 24	Sequence analysisAdd BLAST		24
ChainⁱPRO_0000011907	25 – 897	Probable bifunctional chitinase/lysozymeAdd BLAST		873

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	628 ↔ 673	By similarity

Keywords - PTMⁱ

Disulfide bond

Proteomic databases

PaxDbⁱ	P13656
PRIDEⁱ	P13656

Expressionⁱ

Inductionⁱ

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4261016, 167 interactors
Database of interacting proteins More...DIPⁱ	DIP-9276N
IntActⁱ	P13656, 2 interactors
STRINGⁱ	316407.85676703

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P13656
SMRⁱ	P13656
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	25 – 91	Chitin-binding type-3 1Add BLAST	67
Domainⁱ	128 – 194	Chitin-binding type-3 2Add BLAST	67
Domainⁱ	229 – 295	Chitin-binding type-3 3Add BLAST	67
Domainⁱ	337 – 403	Chitin-binding type-3 4Add BLAST	67
Domainⁱ	459 – 529	Chitin-binding type-3 5Add BLAST	71

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	647 – 897	CatalyticBy similarityAdd BLAST		251

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	93 – 101	Poly-Ser		9

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.Curated

Keywords - Domainⁱ

Repeat, Signal

Phylogenomic databases

eggNOGⁱ	ENOG4108NNZ Bacteria ENOG4111APV LUCA
HOGENOMⁱ	HOG000125409
InParanoidⁱ	P13656
KEGG Orthology (KO) More...KOⁱ	K13381

Family and domain databases

InterProⁱ	View protein in InterPro IPR003610 CBM_fam5/12 IPR036573 CBM_sf_5/12 IPR017853 Glycoside_hydrolase_SF
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02839 CBM_5_12, 1 hit
SMARTⁱ	View protein in SMART SM00495 ChtBD3, 7 hits
SUPFAMⁱ	SSF51055 SSF51055, 1 hit SSF51445 SSF51445, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P13656-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLNIFTKSM IGMGLVCSAL PALAMEAWNN QQGGNKYQVI FDGKIYENAW 
        60         70         80         90        100
WVSSTNCPGK AKANDATNPW RLKRTATAAE ISQFGNTLSC EKSGSSSSSN 
       110        120        130        140        150
SNTPASNTPA NGGSATPAQG TVPSNSSVVA WNKQQGGQTW YVVFNGAVYK 
       160        170        180        190        200
NAWWVASSNC PGDAKSNDAS NPWRYVRAAT ATEISETSNP QSCTSAPQPS 
       210        220        230        240        250
PDVKPAPDVK PAPDVQPAPA DKSNDNYAVV AWKGQEGSST WYVIYNGGIY 
       260        270        280        290        300
KNAWWVGAAN CPGDAKENDA SNPWRYVRAA TATEISQYGN PGSCSVKPDN 
       310        320        330        340        350
NGGAVTPVDP TPETPVTPTP DNSEPSTPAD SVNDYSLQAW SGQEGSEIYH 
       360        370        380        390        400
VIFNGNVYKN AWWVGSKDCP RGTSAENSNN PWRLERTATA AELSQYGNPT 
       410        420        430        440        450
TCEIDNGGVI VADGFQASKA YSADSIVDYN DAHYKTSVDQ DAWGFVPGGD 
       460        470        480        490        500
NPWKKYEPAK AWSASTVYVK GDRVVVDGQA YEALFWTQSD NPALVANQNA 
       510        520        530        540        550
TGSNSRPWKP LGKAQSYSNE ELNNAPQFNP ETLYASDTLI RFNGVNYISQ 
       560        570        580        590        600
SKVQKVSPSD SNPWRVFVDW TGTKERVGTP KKAWPKHVYA PYVDFTLNTI 
       610        620        630        640        650
PDLAALAKNH NVNHFTLAFV VSKDANTCLP TWGTAYGMQN YAQYSKIKAL 
       660        670        680        690        700
REAGGDVMLS IGGANNAPLA ASCKNVDDLM QHYYDIVDNL NLKVLDFDIE 
       710        720        730        740        750
GTWVADQASI ERRNLAVKKV QDKWKSEGKD IAIWYTLPIL PTGLTPEGMN 
       760        770        780        790        800
VLSDAKAKGV ELAGVNVMTM DYGNAICQSA NTEGQNIHGK CATSAIANLH 
       810        820        830        840        850
SQLKGLHPNK SDAEIDAMMG TTPMVGVNDV QGEVFYLSDA RLVMQDAQKR 
       860        870        880        890 
NLGMVGIWSI ARDLPGGTNL SPEFHGLTKE QAPKYAFSEI FAPFTKQ

Length:897

Mass (Da):97,058

Last modified:November 1, 1995 - v2

Checksum:ⁱ968D145BA1F954F3

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	874	F → I in AAC13985 (PubMed:2661540).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U18997 Genomic DNA Translation: AAA58135.1 U00096 Genomic DNA Translation: AAC76363.1 AP009048 Genomic DNA Translation: BAE77953.1 M27176 mRNA Translation: AAC13985.1
PIRⁱ	E65127
NCBI Reference Sequences More...RefSeqⁱ	NP_417797.1, NC_000913.3 WP_000773156.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76363; AAC76363; b3338 BAE77953; BAE77953; BAE77953
GeneIDⁱ	947837
KEGGⁱ	ecj:JW3300 eco:b3338
PATRICⁱ	fig\|1411691.4.peg.3393

Protein	Similar proteins		Species	Score	Length	Source
P13656	Bifunctional chitinase/lysozyme		ECOLX		897	UniRef100_P13656
Bifunctional chitinase/lysozyme		SHIBO		897
Bifunctional chitinase/lysozyme		SHIDY		897
Uncharacterized protein		Escherichia coli UMNK88		897
Putative chitinase/lysozyme		Escherichia coli DEC6A		897
+13

Protein	Similar proteins		Species	Score	Length	Source
P13656	Bifunctional chitinase/lysozyme		ECOLX		897	UniRef90_P13656
Bifunctional chitinase/lysozyme		SHIBO		897
Bifunctional chitinase/lysozyme		SHIDY		897
Uncharacterized protein		Escherichia coli UMNK88		897
Putative chitinase/lysozyme		Escherichia coli DEC6A		897
+644

Protein	Similar proteins		Species	Score	Length	Source
P13656	Bifunctional chitinase/lysozyme		ECOLX		897	UniRef50_P13656
Bifunctional chitinase/lysozyme		SHIBO		897
Bifunctional chitinase/lysozyme		SHIDY		897
Uncharacterized protein		Escherichia coli UMNK88		897
Putative chitinase/lysozyme		Escherichia coli DEC6A		897
+710

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U18997 Genomic DNA Translation: AAA58135.1 U00096 Genomic DNA Translation: AAC76363.1 AP009048 Genomic DNA Translation: BAE77953.1 M27176 mRNA Translation: AAC13985.1
PIRⁱ	E65127
RefSeqⁱ	NP_417797.1, NC_000913.3 WP_000773156.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P13656
SMRⁱ	P13656
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261016, 167 interactors
DIPⁱ	DIP-9276N
IntActⁱ	P13656, 2 interactors
STRINGⁱ	316407.85676703

Protein family/group databases

CAZyⁱ	CBM5 Carbohydrate-Binding Module Family 5 GH18 Glycoside Hydrolase Family 18

CAZyⁱ

CBM5 Carbohydrate-Binding Module Family 5
GH18 Glycoside Hydrolase Family 18

Proteomic databases

PaxDbⁱ	P13656
PRIDEⁱ	P13656

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76363; AAC76363; b3338 BAE77953; BAE77953; BAE77953
GeneIDⁱ	947837
KEGGⁱ	ecj:JW3300 eco:b3338
PATRICⁱ	fig\|1411691.4.peg.3393

Organism-specific databases

EchoBASEⁱ	EB1219
EcoGeneⁱ	EG11237 chiA

Phylogenomic databases

eggNOGⁱ	ENOG4108NNZ Bacteria ENOG4111APV LUCA
HOGENOMⁱ	HOG000125409
InParanoidⁱ	P13656
KOⁱ	K13381

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11237-MONOMER MetaCyc:EG11237-MONOMER

BioCycⁱ

EcoCyc:EG11237-MONOMER
MetaCyc:EG11237-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P13656

PROⁱ

PR:P13656

Family and domain databases

InterProⁱ	View protein in InterPro IPR003610 CBM_fam5/12 IPR036573 CBM_sf_5/12 IPR017853 Glycoside_hydrolase_SF
Pfamⁱ	View protein in Pfam PF02839 CBM_5_12, 1 hit
SMARTⁱ	View protein in SMART SM00495 ChtBD3, 7 hits
SUPFAMⁱ	SSF51055 SSF51055, 1 hit SSF51445 SSF51445, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CHIA_ECOLI
Accessionⁱ	P13656Primary (citable) accession number: P13656 Secondary accession number(s): Q2M703
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
	Last sequence update:	November 1, 1995
	Last modified:	November 7, 2018
	This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P13656 (CHIA_ECOLI)

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Probable bifunctional chitinase/lysozyme

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

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GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

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Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ