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Protein

Probable bifunctional chitinase/lysozyme

Gene

chiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with lysozyme/chitinase activity.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei700Proton donorBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: InterPro
  • chitin binding Source: UniProtKB-KW
  • endochitinase activity Source: EcoCyc
  • lysozyme activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Multifunctional enzyme
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandChitin-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11237-MONOMER
MetaCyc:EG11237-MONOMER

Protein family/group databases

CAZyiCBM5 Carbohydrate-Binding Module Family 5
GH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional chitinase/lysozyme
Including the following 2 domains:
Chitinase (EC:3.2.1.14)
Lysozyme (EC:3.2.1.17)
Gene namesi
Name:chiA
Synonyms:yheB
Ordered Locus Names:b3338, JW3300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11237 chiA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001190725 – 897Probable bifunctional chitinase/lysozymeAdd BLAST873

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi628 ↔ 673By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP13656
PRIDEiP13656

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261016, 167 interactors
DIPiDIP-9276N
IntActiP13656, 2 interactors
STRINGi316407.85676703

Structurei

3D structure databases

ProteinModelPortaliP13656
SMRiP13656
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 91Chitin-binding type-3 1Add BLAST67
Domaini128 – 194Chitin-binding type-3 2Add BLAST67
Domaini229 – 295Chitin-binding type-3 3Add BLAST67
Domaini337 – 403Chitin-binding type-3 4Add BLAST67
Domaini459 – 529Chitin-binding type-3 5Add BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni647 – 897CatalyticBy similarityAdd BLAST251

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi93 – 101Poly-Ser9

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108NNZ Bacteria
ENOG4111APV LUCA
HOGENOMiHOG000125409
InParanoidiP13656
KOiK13381

Family and domain databases

InterProiView protein in InterPro
IPR003610 CBM_fam5/12
IPR036573 CBM_sf_5/12
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF02839 CBM_5_12, 1 hit
SMARTiView protein in SMART
SM00495 ChtBD3, 7 hits
SUPFAMiSSF51055 SSF51055, 1 hit
SSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13656-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLNIFTKSM IGMGLVCSAL PALAMEAWNN QQGGNKYQVI FDGKIYENAW
60 70 80 90 100
WVSSTNCPGK AKANDATNPW RLKRTATAAE ISQFGNTLSC EKSGSSSSSN
110 120 130 140 150
SNTPASNTPA NGGSATPAQG TVPSNSSVVA WNKQQGGQTW YVVFNGAVYK
160 170 180 190 200
NAWWVASSNC PGDAKSNDAS NPWRYVRAAT ATEISETSNP QSCTSAPQPS
210 220 230 240 250
PDVKPAPDVK PAPDVQPAPA DKSNDNYAVV AWKGQEGSST WYVIYNGGIY
260 270 280 290 300
KNAWWVGAAN CPGDAKENDA SNPWRYVRAA TATEISQYGN PGSCSVKPDN
310 320 330 340 350
NGGAVTPVDP TPETPVTPTP DNSEPSTPAD SVNDYSLQAW SGQEGSEIYH
360 370 380 390 400
VIFNGNVYKN AWWVGSKDCP RGTSAENSNN PWRLERTATA AELSQYGNPT
410 420 430 440 450
TCEIDNGGVI VADGFQASKA YSADSIVDYN DAHYKTSVDQ DAWGFVPGGD
460 470 480 490 500
NPWKKYEPAK AWSASTVYVK GDRVVVDGQA YEALFWTQSD NPALVANQNA
510 520 530 540 550
TGSNSRPWKP LGKAQSYSNE ELNNAPQFNP ETLYASDTLI RFNGVNYISQ
560 570 580 590 600
SKVQKVSPSD SNPWRVFVDW TGTKERVGTP KKAWPKHVYA PYVDFTLNTI
610 620 630 640 650
PDLAALAKNH NVNHFTLAFV VSKDANTCLP TWGTAYGMQN YAQYSKIKAL
660 670 680 690 700
REAGGDVMLS IGGANNAPLA ASCKNVDDLM QHYYDIVDNL NLKVLDFDIE
710 720 730 740 750
GTWVADQASI ERRNLAVKKV QDKWKSEGKD IAIWYTLPIL PTGLTPEGMN
760 770 780 790 800
VLSDAKAKGV ELAGVNVMTM DYGNAICQSA NTEGQNIHGK CATSAIANLH
810 820 830 840 850
SQLKGLHPNK SDAEIDAMMG TTPMVGVNDV QGEVFYLSDA RLVMQDAQKR
860 870 880 890
NLGMVGIWSI ARDLPGGTNL SPEFHGLTKE QAPKYAFSEI FAPFTKQ
Length:897
Mass (Da):97,058
Last modified:November 1, 1995 - v2
Checksum:i968D145BA1F954F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti874F → I in AAC13985 (PubMed:2661540).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58135.1
U00096 Genomic DNA Translation: AAC76363.1
AP009048 Genomic DNA Translation: BAE77953.1
M27176 mRNA Translation: AAC13985.1
PIRiE65127
RefSeqiNP_417797.1, NC_000913.3
WP_000773156.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76363; AAC76363; b3338
BAE77953; BAE77953; BAE77953
GeneIDi947837
KEGGiecj:JW3300
eco:b3338
PATRICifig|1411691.4.peg.3393

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58135.1
U00096 Genomic DNA Translation: AAC76363.1
AP009048 Genomic DNA Translation: BAE77953.1
M27176 mRNA Translation: AAC13985.1
PIRiE65127
RefSeqiNP_417797.1, NC_000913.3
WP_000773156.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP13656
SMRiP13656
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261016, 167 interactors
DIPiDIP-9276N
IntActiP13656, 2 interactors
STRINGi316407.85676703

Protein family/group databases

CAZyiCBM5 Carbohydrate-Binding Module Family 5
GH18 Glycoside Hydrolase Family 18

Proteomic databases

PaxDbiP13656
PRIDEiP13656

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76363; AAC76363; b3338
BAE77953; BAE77953; BAE77953
GeneIDi947837
KEGGiecj:JW3300
eco:b3338
PATRICifig|1411691.4.peg.3393

Organism-specific databases

EchoBASEiEB1219
EcoGeneiEG11237 chiA

Phylogenomic databases

eggNOGiENOG4108NNZ Bacteria
ENOG4111APV LUCA
HOGENOMiHOG000125409
InParanoidiP13656
KOiK13381

Enzyme and pathway databases

BioCyciEcoCyc:EG11237-MONOMER
MetaCyc:EG11237-MONOMER

Miscellaneous databases

PROiPR:P13656

Family and domain databases

InterProiView protein in InterPro
IPR003610 CBM_fam5/12
IPR036573 CBM_sf_5/12
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF02839 CBM_5_12, 1 hit
SMARTiView protein in SMART
SM00495 ChtBD3, 7 hits
SUPFAMiSSF51055 SSF51055, 1 hit
SSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHIA_ECOLI
AccessioniPrimary (citable) accession number: P13656
Secondary accession number(s): Q2M703
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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