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UniProtKB - P13650 (DHGB_ACICA)
Protein
Quinoprotein glucose dehydrogenase B
Gene
gdhB
Organism
Acinetobacter calcoaceticus
Status
Functioni
Oxidizes glucose to gluconolactone.
Miscellaneous
Acinetobacter calcoaceticus contains two different PQQ dependent enzymes with GDH activity. GDH-A prefers 2-deoxyglucose as substrate, the specific substrates for GDH-B are disaccharides.
Catalytic activityi
- EC:1.1.5.2
Cofactori
Protein has several cofactor binding sites:- pyrroloquinoline quinoneNote: Binds 1 PQQ group per subunit.
- Ca2+Note: Binds 3 Ca2+ ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 100 | Glucose1 Publication | 1 | |
Binding sitei | 167 | Glucose1 Publication | 1 | |
Active sitei | 168 | Proton acceptor | 1 | |
Binding sitei | 192 | Glucose1 Publication | 1 | |
Binding sitei | 252 | Glucose1 Publication | 1 | |
Metal bindingi | 271 | Calcium 1; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 272 | Calcium 1; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 277 | Calcium 21 Publication | 1 | |
Metal bindingi | 287 | Calcium 2; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 293 | Calcium 3; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 295 | Calcium 3; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 297 | Calcium 31 Publication | 1 | |
Metal bindingi | 333 | Calcium 31 Publication | 1 | |
Binding sitei | 367 | PQQ; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 372 | PQQ1 Publication | 1 | |
Binding sitei | 401 | PQQ1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- quinoprotein glucose dehydrogenase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Calcium, Metal-binding, PQQ |
Enzyme and pathway databases
BRENDAi | 1.1.5.2, 99 1.1.99.35, 99 |
Names & Taxonomyi
Protein namesi | Recommended name: Quinoprotein glucose dehydrogenase B (EC:1.1.5.2)Alternative name(s): Glucose dehydrogenase B [pyrroloquinoline-quinone] Soluble glucose dehydrogenase Short name: s-GDH |
Gene namesi | Name:gdhB |
Organismi | Acinetobacter calcoaceticus |
Taxonomic identifieri | 471 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Moraxellales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex |
Pathology & Biotechi
Biotechnological usei
Potent biocatalyst for the accurate in vivo monitoring of blood glucose in the management of diabetes.
Chemistry databases
DrugBanki | DB02379, Beta-D-Glucose DB04361, Methyldiazene DB03205, Pyrroloquinoline Quinone |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Add BLAST | 24 | |
ChainiPRO_0000025583 | 25 – 478 | Quinoprotein glucose dehydrogenase BAdd BLAST | 454 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
STRINGi | 471.BUM88_11175 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P13650 |
SMRi | P13650 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13650 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 252 – 253 | PQQ | 2 | |
Regioni | 430 – 432 | PQQ | 3 |
Sequence similaritiesi
Belongs to the PQQ oxidoreductase GdhB family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 2.120.10.30, 1 hit |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR012938, Glc/Sorbosone_DH IPR019893, PQQ-dependent_DH_s-GDH IPR011041, Quinoprot_gluc/sorb_DH |
Pfami | View protein in Pfam PF07995, GSDH, 2 hits |
SUPFAMi | SSF50952, SSF50952, 1 hit |
TIGRFAMsi | TIGR03606, non_repeat_PQQ, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P13650-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKHLLAKIA LLSAVQLVTL SAFADVPLTP SQFAKAKSEN FDKKVILSNL
60 70 80 90 100
NKPHALLWGP DNQIWLTERA TGKILRVNPE SGSVKTVFQV PEIVNDADGQ
110 120 130 140 150
NGLLGFAFHP DFKNNPYIYI SGTFKNPKST DKELPNQTII RRYTYNKSTD
160 170 180 190 200
TLEKPVDLLA GLPSSKDHQS GRLVIGPDQK IYYTIGDQGR NQLAYLFLPN
210 220 230 240 250
QAQHTPTQQE LNGKDYHTYM GKVLRLNLDG SIPKDNPSFN GVVSHIYTLG
260 270 280 290 300
HRNPQGLAFT PNGKLLQSEQ GPNSDDEINL IVKGGNYGWP NVAGYKDDSG
310 320 330 340 350
YAYANYSAAA NKSIKDLAQN GVKVAAGVPV TKESEWTGKN FVPPLKTLYT
360 370 380 390 400
VQDTYNYNDP TCGEMTYICW PTVAPSSAYV YKGGKKAITG WENTLLVPSL
410 420 430 440 450
KRGVIFRIKL DPTYSTTYDD AVPMFKSNNR YRDVIASPDG NVLYVLTDTA
460 470
GNVQKDDGSV TNTLENPGSL IKFTYKAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15871 Genomic DNA Translation: CAA33881.1 |
PIRi | S04784 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15871 Genomic DNA Translation: CAA33881.1 |
PIRi | S04784 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C9U | X-ray | 2.20 | A/B | 25-478 | [»] | |
1CQ1 | X-ray | 1.90 | A/B | 25-478 | [»] | |
1CRU | X-ray | 1.50 | A/B | 25-478 | [»] | |
1QBI | X-ray | 1.72 | A/B | 25-478 | [»] | |
5MIN | X-ray | 1.76 | A/B | 25-477 | [»] | |
AlphaFoldDBi | P13650 | |||||
SMRi | P13650 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 471.BUM88_11175 |
Chemistry databases
DrugBanki | DB02379, Beta-D-Glucose DB04361, Methyldiazene DB03205, Pyrroloquinoline Quinone |
Enzyme and pathway databases
BRENDAi | 1.1.5.2, 99 1.1.99.35, 99 |
Miscellaneous databases
EvolutionaryTracei | P13650 |
Family and domain databases
Gene3Di | 2.120.10.30, 1 hit |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR012938, Glc/Sorbosone_DH IPR019893, PQQ-dependent_DH_s-GDH IPR011041, Quinoprot_gluc/sorb_DH |
Pfami | View protein in Pfam PF07995, GSDH, 2 hits |
SUPFAMi | SSF50952, SSF50952, 1 hit |
TIGRFAMsi | TIGR03606, non_repeat_PQQ, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHGB_ACICA | |
Accessioni | P13650Primary (citable) accession number: P13650 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families