UniProtKB - P13607 (ATNA_DROME)
Sodium/potassium-transporting ATPase subunit alpha
Atpalpha
Functioni
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
3 PublicationsMiscellaneous
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 394 | 4-aspartylphosphate intermediateCurated | 1 | |
Binding sitei | 526 | ATPBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- P-type sodium:potassium-exchanging transporter activity Source: FlyBase
GO - Biological processi
- adult locomotory behavior Source: FlyBase
- cation transport Source: FlyBase
- cellular potassium ion homeostasis Source: GO_Central
- cellular sodium ion homeostasis Source: GO_Central
- chemical synaptic transmission Source: FlyBase
- determination of adult lifespan Source: FlyBase
- jump response Source: FlyBase
- locomotory behavior Source: FlyBase
- neuromuscular process Source: FlyBase
- potassium ion import across plasma membrane Source: GO_Central
- proton transmembrane transport Source: GO_Central
- regulation of tube diameter, open tracheal system Source: FlyBase
- regulation of tube length, open tracheal system Source: FlyBase
- response to mechanical stimulus Source: FlyBase
- response to temperature stimulus Source: FlyBase
- sensory perception of sound Source: FlyBase
- septate junction assembly Source: FlyBase
- sodium ion export across plasma membrane Source: GO_Central
- synaptic assembly at neuromuscular junction Source: FlyBase
- tissue homeostasis Source: FlyBase
- trachea morphogenesis Source: FlyBase
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | ATP-binding, Nucleotide-binding, Potassium, Sodium |
Enzyme and pathway databases
Reactomei | R-DME-936837, Ion transport by P-type ATPases |
SignaLinki | P13607 |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/potassium-transporting ATPase subunit alpha (EC:7.2.2.131 Publication)Short name: Na(+)/K(+) ATPase alpha subunit Alternative name(s): Sodium pump subunit alpha |
Gene namesi | Name:Atpalpha Synonyms:Na-p ORF Names:CG5670 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002921, Atpalpha |
VEuPathDBi | VectorBase:FBgn0002921 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein
Nucleus
- nucleus Source: FlyBase
Plasma Membrane
- basolateral plasma membrane Source: FlyBase
- plasma membrane Source: FlyBase
- sodium:potassium-exchanging ATPase complex Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- septate junction Source: FlyBase
- synapse Source: GOC
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 147 – 167 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 312 – 332 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 338 – 358 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 808 – 828 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 870 – 890 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 935 – 955 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 970 – 990 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1020 | D → N in allele ATP-alpha-dts2; homozygous lethal and temperature dependent bang sensitive paralysis, shortened life span and neurodegeneration when heterozygous. 1 Publication | 1 | |
Mutagenesisi | 1021 | E → K in allele ATP-alpha-dts1; homozygous lethal and temperature dependent bang sensitive paralysis, shortened life span and neurodegeneration when heterozygous. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046309 | 1 – 1041 | Sodium/potassium-transporting ATPase subunit alphaAdd BLAST | 1041 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P13607 |
PRIDEi | P13607 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | FBgn0002921, Expressed in adult Malpighian tubule (Drosophila) and 37 other tissues |
ExpressionAtlasi | P13607, baseline and differential |
Genevisiblei | P13607, DM |
Interactioni
Subunit structurei
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
CuratedProtein-protein interaction databases
BioGRIDi | 71866, 59 interactors |
DIPi | DIP-19649N |
IntActi | P13607, 36 interactors |
STRINGi | 7227.FBpp0088502 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0203, Eukaryota |
InParanoidi | P13607 |
OMAi | FINLVTD |
PhylomeDBi | P13607 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALRSDYEHG RADSYRVATV IATDDDNRTA DGQYKSRRKM PAKVNKKENL
60 70 80 90 100
DDLKQELDID FHKISPEELY QRFQTHPENG LSHAKAKENL ERDGPNALTP
110 120 130 140 150
PKQTPEWVKF CKNLFGGFAM LLWIGAILCF VAYSIQASTS EEPADDNLYL
160 170 180 190 200
GIVLSAVVIV TGIFSYYQES KSSKIMESFK NMVPQFATVI REGEKLTLRA
210 220 230 240 250
EDLVLGDVVE VKFGDRIPAD IRIIEARNFK VDNSSLTGES EPQSRGAEFT
260 270 280 290 300
HENPLETKNL AFFSTNAVEG TAKGVVISCG DHTVMGRIAG LASGLDTGET
310 320 330 340 350
PIAKEIHHFI HLITGVAVFL GVTFFVIAFI LGYHWLDAVI FLIGIIVANV
360 370 380 390 400
PEGLLATVTV CLTLTAKRMA SKNCLVKNLE AVETLGSTST ICSDKTGTLT
410 420 430 440 450
QNRMTVAHMW FDNQIIEADT TEDQSGVQYD RTSPGFKALS RIATLCNRAE
460 470 480 490 500
FKGGQDGVPI LKKEVSGDAS EAALLKCMEL ALGDVMNIRK RNKKIAEVPF
510 520 530 540 550
NSTNKYQVSI HETEDTNDPR YLLVMKGAPE RILERCSTIF INGKEKVLDE
560 570 580 590 600
EMKEAFNNAY MELGGLGERV LGFCDFMLPS DKYPNGFKFN TDDINFPIDN
610 620 630 640 650
LRFVGLMSMI DPPRAAVPDA VAKCRSAGIK VIMVTGDHPI TAKAIAKSVG
660 670 680 690 700
IISEGNETVE DIAQRLNIPV SEVNPREAKA AVVHGAELRD VSSDQLDEIL
710 720 730 740 750
RYHTEIVFAR TSPQQKLIIV EGCQRMGAIV AVTGDGVNDS PALKKADIGV
760 770 780 790 800
AMGIAGSDVS KQAADMILLD DNFASIVTGV EEGRLIFDNL KKSIAYTLTS
810 820 830 840 850
NIPEISPFLA FILCDIPLPL GTVTILCIDL GTDMVPAISL AYEHAEADIM
860 870 880 890 900
KRPPRDPFND KLVNSRLISM AYGQIGMIQA AAGFFVYFVI MAENGFLPKK
910 920 930 940 950
LFGIRKMWDS KAVNDLTDSY GQEWTYRDRK TLEYTCHTAF FISIVVVQWA
960 970 980 990 1000
DLIICKTRRN SIFQQGMRNW ALNFGLVFET VLAAFLSYCP GMEKGLRMYP
1010 1020 1030 1040
LKLVWWFPAI PFALAIFIYD ETRRFYLRRN PGGWLEQETY Y
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE1JIR4 | E1JIR4_DROME | Sodium/potassium-transporting ATPas... | Atpalpha (Na[+], alpha-ATPase, Atp, Atp a, ATP-alpha | 1,002 | Annotation score: | ||
A0A0B4KGG8 | A0A0B4KGG8_DROME | Sodium/potassium-transporting ATPas... | Atpalpha (Na[+], alpha-ATPase, Atp, Atp a, ATP-alpha | 1,002 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 | L → M in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 85 | K → R in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 97 | Missing in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 112 – 113 | KN → ED in CAA32638 (PubMed:2540956).Curated | 2 | |
Sequence conflicti | 117 – 118 | GF → V in CAA32638 (PubMed:2540956).Curated | 2 | |
Sequence conflicti | 163 | I → V in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 192 | E → G in AAC05260 (PubMed:9648860).Curated | 1 | |
Sequence conflicti | 196 – 197 | LT → PS in CAA32638 (PubMed:2540956).Curated | 2 | |
Sequence conflicti | 207 – 208 | DV → VL in CAA32638 (PubMed:2540956).Curated | 2 | |
Sequence conflicti | 211 – 212 | VK → LE in CAA32638 (PubMed:2540956).Curated | 2 | |
Sequence conflicti | 216 – 221 | RIPADI → LIPLVY in CAA32638 (PubMed:2540956).Curated | 6 | |
Sequence conflicti | 228 | N → D in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 270 – 272 | GTA → ALP in CAA32638 (PubMed:2540956).Curated | 3 | |
Sequence conflicti | 290 | G → A in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 299 | Missing in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 402 | N → T in CAA35504 (PubMed:2557235).Curated | 1 | |
Sequence conflicti | 488 | I → N in CAA35504 (PubMed:2557235).Curated | 1 | |
Sequence conflicti | 811 | F → S in CAA32638 (PubMed:2540956).Curated | 1 | |
Sequence conflicti | 843 | E → D in CAA32638 (PubMed:2540956).Curated | 1 |
RNA editingi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 429 | Y → C in RNA edited version. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000417 | 1 – 39 | Missing in isoform 2, isoform 3, isoform 6 and isoform 7. 2 PublicationsAdd BLAST | 39 | |
Alternative sequenceiVSP_008074 | 1 – 8 | MALRSDYE → MSAQ in isoform 4. Curated | 8 | |
Alternative sequenceiVSP_008075 | 835 – 865 | VPAIS…KLVNS → IPAISLAYEGPEADIMKRRP RNPEIDNLVNE in isoform 5. CuratedAdd BLAST | 31 | |
Alternative sequenceiVSP_008076 | 835 – 865 | VPAIS…KLVNS → IPAISLAYEQAESDIMKRQP RDPYRDNLVNR in isoform 7. CuratedAdd BLAST | 31 | |
Alternative sequenceiVSP_008077 | 836 – 878 | PAISL…QIGMI → SLDLCPKPKLHRRLKLKLLL SQYHSSKLYSYTSCSPSQLI VKN in isoform 3. 1 PublicationAdd BLAST | 43 | |
Alternative sequenceiVSP_000418 | 844 – 865 | HAEAD…KLVNS → TAESDIMKRQPRNPFQDKLV NE in isoform 6. CuratedAdd BLAST | 22 | |
Alternative sequenceiVSP_008078 | 879 – 1041 | Missing in isoform 3. 1 PublicationAdd BLAST | 163 |
Sequence databases
Genome annotation databases
EnsemblMetazoai | FBtr0089509; FBpp0088501; FBgn0002921 [P13607-2] FBtr0089510; FBpp0088502; FBgn0002921 [P13607-1] FBtr0089511; FBpp0088503; FBgn0002921 [P13607-2] FBtr0089512; FBpp0088504; FBgn0002921 [P13607-3] FBtr0089513; FBpp0088984; FBgn0002921 [P13607-2] FBtr0089514; FBpp0088983; FBgn0002921 [P13607-6] FBtr0089515; FBpp0088982; FBgn0002921 [P13607-7] FBtr0089516; FBpp0088981; FBgn0002921 [P13607-6] FBtr0333324; FBpp0305516; FBgn0002921 [P13607-2] FBtr0333325; FBpp0305517; FBgn0002921 [P13607-2] |
GeneIDi | 48971 |
KEGGi | dme:Dmel_CG5670 |
UCSCi | CG5670-RE, d. melanogaster CG5670-RH, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicing, RNA editingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | P13607 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 71866, 59 interactors |
DIPi | DIP-19649N |
IntActi | P13607, 36 interactors |
STRINGi | 7227.FBpp0088502 |
Proteomic databases
PaxDbi | P13607 |
PRIDEi | P13607 |
Protocols and materials databases
DNASUi | 48971 |
Genome annotation databases
EnsemblMetazoai | FBtr0089509; FBpp0088501; FBgn0002921 [P13607-2] FBtr0089510; FBpp0088502; FBgn0002921 [P13607-1] FBtr0089511; FBpp0088503; FBgn0002921 [P13607-2] FBtr0089512; FBpp0088504; FBgn0002921 [P13607-3] FBtr0089513; FBpp0088984; FBgn0002921 [P13607-2] FBtr0089514; FBpp0088983; FBgn0002921 [P13607-6] FBtr0089515; FBpp0088982; FBgn0002921 [P13607-7] FBtr0089516; FBpp0088981; FBgn0002921 [P13607-6] FBtr0333324; FBpp0305516; FBgn0002921 [P13607-2] FBtr0333325; FBpp0305517; FBgn0002921 [P13607-2] |
GeneIDi | 48971 |
KEGGi | dme:Dmel_CG5670 |
UCSCi | CG5670-RE, d. melanogaster CG5670-RH, d. melanogaster |
Organism-specific databases
CTDi | 48971 |
FlyBasei | FBgn0002921, Atpalpha |
VEuPathDBi | VectorBase:FBgn0002921 |
Phylogenomic databases
eggNOGi | KOG0203, Eukaryota |
InParanoidi | P13607 |
OMAi | FINLVTD |
PhylomeDBi | P13607 |
Enzyme and pathway databases
Reactomei | R-DME-936837, Ion transport by P-type ATPases |
SignaLinki | P13607 |
Miscellaneous databases
BioGRID-ORCSi | 48971, 1 hit in 3 CRISPR screens |
ChiTaRSi | Atpalpha, fly |
GenomeRNAii | 48971 |
PROi | PR:P13607 |
Gene expression databases
Bgeei | FBgn0002921, Expressed in adult Malpighian tubule (Drosophila) and 37 other tissues |
ExpressionAtlasi | P13607, baseline and differential |
Genevisiblei | P13607, DM |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATNA_DROME | |
Accessioni | P13607Primary (citable) accession number: P13607 Secondary accession number(s): A4V366 Q9VDG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | June 20, 2001 | |
Last modified: | February 23, 2022 | |
This is version 214 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families