UniProtKB - P13597 (ICAM1_MOUSE)
Intercellular adhesion molecule 1
Icam1
Functioni
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
By similarityGO - Molecular functioni
- integrin binding Source: MGI
- protein-containing complex binding Source: MGI
GO - Biological processi
- acute inflammatory response to antigenic stimulus Source: MGI
- adhesion of symbiont to host Source: MGI
- cell adhesion Source: MGI
- cell adhesion mediated by integrin Source: MGI
- cell-cell adhesion Source: MGI
- cellular response to amyloid-beta Source: MGI
- cellular response to glucose stimulus Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- establishment of endothelial barrier Source: MGI
- establishment of endothelial intestinal barrier Source: MGI
- establishment of Sertoli cell barrier Source: MGI
- leukocyte cell-cell adhesion Source: MGI
- membrane to membrane docking Source: Ensembl
- negative regulation of calcium ion transport Source: MGI
- negative regulation of endothelial cell apoptotic process Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- positive regulation of actin filament polymerization Source: MGI
- positive regulation of cellular extravasation Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of GTPase activity Source: MGI
- positive regulation of leukocyte adhesion to vascular endothelial cell Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
- positive regulation of vasoconstriction Source: MGI
- receptor-mediated virion attachment to host cell Source: MGI
- regulation of cell shape Source: MGI
- regulation of ruffle assembly Source: MGI
- sensory perception of sound Source: MGI
- T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: MGI
- T cell antigen processing and presentation Source: MGI
- T cell extravasation Source: MGI
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-216083, Integrin cell surface interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Intercellular adhesion molecule 1Short name: ICAM-1 Alternative name(s): MALA-2 MyD10 CD_antigen: CD54 |
Gene namesi | Name:Icam1 Synonyms:Icam-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96392, Icam1 |
VEuPathDBi | HostDB:ENSMUSG00000037405 |
Subcellular locationi
Other locations
Extracellular region or secreted
- extracellular exosome Source: MGI
- extracellular space Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
- immunological synapse Source: MGI
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 485 | ExtracellularSequence analysisAdd BLAST | 458 | |
Transmembranei | 486 – 509 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 510 – 537 | CytoplasmicSequence analysisAdd BLAST | 28 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | By similarityAdd BLAST | 27 | |
ChainiPRO_0000014785 | 28 – 537 | Intercellular adhesion molecule 1Add BLAST | 510 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 47 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 48 ↔ 91 | PROSITE-ProRule annotation | ||
Disulfide bondi | 52 ↔ 95 | PROSITE-ProRule annotation | ||
Disulfide bondi | 134 ↔ 188 | PROSITE-ProRule annotation | ||
Glycosylationi | 185 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Glycosylationi | 204 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 239 ↔ 292 | PROSITE-ProRule annotation | ||
Glycosylationi | 267 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 311 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 334 ↔ 374 | PROSITE-ProRule annotation | ||
Glycosylationi | 362 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Glycosylationi | 388 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 406 ↔ 422 | By similarity | ||
Glycosylationi | 409 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 422 ↔ 461 | PROSITE-ProRule annotation | ||
Disulfide bondi | 434 ↔ 461 | By similarity | ||
Glycosylationi | 456 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 469 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 469 | Not glycosylated1 Publication | 1 | |
Sitei | 485 | Not glycosylated1 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3362 |
EPDi | P13597 |
jPOSTi | P13597 |
MaxQBi | P13597 |
PaxDbi | P13597 |
PeptideAtlasi | P13597 |
PRIDEi | P13597 |
ProteomicsDBi | 269522 [P13597-1] 269523 [P13597-2] |
PTM databases
GlyGeni | P13597, 10 sites |
iPTMneti | P13597 |
PhosphoSitePlusi | P13597 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000037405, Expressed in lung and 236 other tissues |
ExpressionAtlasi | P13597, baseline and differential |
Genevisiblei | P13597, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with MUC1 and promotes cell aggregation in epithelial cells.
Interacts with ARHGEF26/SGEF.
Interacts (on T cell side) with CD81, CD247 and CD9 at immunological synapses between antigen-presenting cells and T cells.
By similarityGO - Molecular functioni
- integrin binding Source: MGI
Protein-protein interaction databases
DIPi | DIP-29096N |
IntActi | P13597, 1 interactor |
STRINGi | 10090.ENSMUSP00000083587 |
Miscellaneous databases
RNActi | P13597, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 102 | Ig-like C2-type 1Add BLAST | 62 | |
Domaini | 127 – 195 | Ig-like C2-type 2Add BLAST | 69 | |
Domaini | 232 – 299 | Ig-like C2-type 3Add BLAST | 68 | |
Domaini | 327 – 381 | Ig-like C2-type 4Add BLAST | 55 | |
Domaini | 415 – 468 | Ig-like C2-type 5Add BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 151 – 153 | Cell attachment site; atypicalSequence analysis | 3 | |
Motifi | 179 – 181 | Cell attachment siteSequence analysis | 3 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S45R, Eukaryota |
GeneTreei | ENSGT00940000162311 |
HOGENOMi | CLU_036160_1_1_1 |
InParanoidi | P13597 |
OMAi | NLTVYWF |
PhylomeDBi | P13597 |
TreeFami | TF333745 |
Family and domain databases
Gene3Di | 2.60.40.10, 5 hits |
InterProi | View protein in InterPro IPR003988, ICAM IPR013768, ICAM_N IPR003987, ICAM_VCAM_N IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub |
Pfami | View protein in Pfam PF03921, ICAM_N, 1 hit PF13895, Ig_2, 1 hit |
PRINTSi | PR01473, ICAM PR01472, ICAMVCAM1 |
SMARTi | View protein in SMART SM00409, IG, 4 hits |
SUPFAMi | SSF48726, SSF48726, 5 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASTRAKPTL PLLLALVTVV IPGPGDAQVS IHPREAFLPQ GGSVQVNCSS
60 70 80 90 100
SCKEDLSLGL ETQWLKDELE SGPNWKLFEL SEIGEDSSPL CFENCGTVQS
110 120 130 140 150
SASATITVYS FPESVELRPL PAWQQVGKDL TLRCHVDGGA PRTQLSAVLL
160 170 180 190 200
RGEEILSRQP VGGHPKDPKE ITFTVLASRG DHGANFSCRT ELDLRPQGLA
210 220 230 240 250
LFSNVSEARS LRTFDLPATI PKLDTPDLLE VGTQQKLFCS LEGLFPASEA
260 270 280 290 300
RIYLELGGQM PTQESTNSSD SVSATALVEV TEEFDRTLPL RCVLELADQI
310 320 330 340 350
LETQRTLTVY NFSAPVLTLS QLEVSEGSQV TVKCEAHSGS KVVLLSGVEP
360 370 380 390 400
RPPTPQVQFT LNASSEDHKR SFFCSAALEV AGKFLFKNQT LELHVLYGPR
410 420 430 440 450
LDETDCLGNW TWQEGSQQTL KCQAWGNPSP KMTCRRKADG ALLPIGVVKS
460 470 480 490 500
VKQEMNGTYV CHAFSSHGNV TRNVYLTVLY HSQNNWTIII LVPVLLVIVG
510 520 530
LVMAASYVYN RQRKIRIYKL QKAQEEAIKL KGQAPPP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 243 | G → A in AAA37876 (PubMed:2479693).Curated | 1 | |
Sequence conflicti | 370 | R → A in CAA34621 (PubMed:2573511).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002518 | 1 – 34 | MASTR…SIHPR → MITHRHPVREKSINSYQFIK EKQFPAEN in isoform 2. CuratedAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16624 mRNA Translation: CAA34621.1 X16625 mRNA Translation: CAA34622.1 M31585 mRNA Translation: AAA37876.1 X52264 mRNA Translation: CAA36507.1 M90551 , M90546, M90547, M90548, M90549, M90550 Genomic DNA Translation: AAA37875.1 X54331 mRNA No translation available. |
CCDSi | CCDS22889.1 [P13597-1] |
PIRi | A45815 I49769 S06016 |
RefSeqi | NP_034623.1, NM_010493.3 [P13597-1] |
Genome annotation databases
Ensembli | ENSMUST00000086399; ENSMUSP00000083587; ENSMUSG00000037405 [P13597-1] |
GeneIDi | 15894 |
KEGGi | mmu:15894 |
UCSCi | uc009ojx.1, mouse [P13597-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding ICAM-1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16624 mRNA Translation: CAA34621.1 X16625 mRNA Translation: CAA34622.1 M31585 mRNA Translation: AAA37876.1 X52264 mRNA Translation: CAA36507.1 M90551 , M90546, M90547, M90548, M90549, M90550 Genomic DNA Translation: AAA37875.1 X54331 mRNA No translation available. |
CCDSi | CCDS22889.1 [P13597-1] |
PIRi | A45815 I49769 S06016 |
RefSeqi | NP_034623.1, NM_010493.3 [P13597-1] |
3D structure databases
AlphaFoldDBi | P13597 |
SMRi | P13597 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-29096N |
IntActi | P13597, 1 interactor |
STRINGi | 10090.ENSMUSP00000083587 |
PTM databases
GlyGeni | P13597, 10 sites |
iPTMneti | P13597 |
PhosphoSitePlusi | P13597 |
Proteomic databases
CPTACi | non-CPTAC-3362 |
EPDi | P13597 |
jPOSTi | P13597 |
MaxQBi | P13597 |
PaxDbi | P13597 |
PeptideAtlasi | P13597 |
PRIDEi | P13597 |
ProteomicsDBi | 269522 [P13597-1] 269523 [P13597-2] |
Protocols and materials databases
Antibodypediai | 795, 3152 antibodies from 53 providers |
DNASUi | 15894 |
Genome annotation databases
Ensembli | ENSMUST00000086399; ENSMUSP00000083587; ENSMUSG00000037405 [P13597-1] |
GeneIDi | 15894 |
KEGGi | mmu:15894 |
UCSCi | uc009ojx.1, mouse [P13597-1] |
Organism-specific databases
CTDi | 3383 |
MGIi | MGI:96392, Icam1 |
VEuPathDBi | HostDB:ENSMUSG00000037405 |
Phylogenomic databases
eggNOGi | ENOG502S45R, Eukaryota |
GeneTreei | ENSGT00940000162311 |
HOGENOMi | CLU_036160_1_1_1 |
InParanoidi | P13597 |
OMAi | NLTVYWF |
PhylomeDBi | P13597 |
TreeFami | TF333745 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-216083, Integrin cell surface interactions |
Miscellaneous databases
BioGRID-ORCSi | 15894, 5 hits in 75 CRISPR screens |
ChiTaRSi | Icam1, mouse |
PROi | PR:P13597 |
RNActi | P13597, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037405, Expressed in lung and 236 other tissues |
ExpressionAtlasi | P13597, baseline and differential |
Genevisiblei | P13597, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 5 hits |
InterProi | View protein in InterPro IPR003988, ICAM IPR013768, ICAM_N IPR003987, ICAM_VCAM_N IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub |
Pfami | View protein in Pfam PF03921, ICAM_N, 1 hit PF13895, Ig_2, 1 hit |
PRINTSi | PR01473, ICAM PR01472, ICAMVCAM1 |
SMARTi | View protein in SMART SM00409, IG, 4 hits |
SUPFAMi | SSF48726, SSF48726, 5 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ICAM1_MOUSE | |
Accessioni | P13597Primary (citable) accession number: P13597 Secondary accession number(s): Q61828 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 212 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families