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Protein

Sodium transport ATPase 1

Gene

ENA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the sodium or lithium ions to allow salt tolerance. Is negatively modulated by SIS2/HAL3.2 Publications

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O + Na+(In) = ADP + phosphate + Na+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3694-aspartylphosphate intermediateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • potassium-transporting ATPase activity Source: SGD
  • sodium-exporting ATPase activity, phosphorylative mechanism Source: SGD

GO - Biological processi

  • cellular response to glucose starvation Source: SGD
  • hyperosmotic response Source: SGD
  • potassium ion transport Source: SGD
  • response to pH Source: SGD
  • response to salt stress Source: UniProtKB
  • sodium ion transport Source: SGD
  • transmembrane transport Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processIon transport, Sodium transport, Transport
LigandATP-binding, Magnesium, Nucleotide-binding, Sodium

Enzyme and pathway databases

BioCyciYEAST:G3O-29654-MONOMER
BRENDAi3.6.3.7 1113

Protein family/group databases

TCDBi3.A.3.9.1 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium transport ATPase 1 (EC:3.6.3.7)
Gene namesi
Name:ENA1
Synonyms:HOR6, PMR2, PMR2A
Ordered Locus Names:YDR040C
ORF Names:YD6888.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR040C
SGDiS000002447 ENA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63CytoplasmicSequence analysisAdd BLAST63
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 90ExtracellularSequence analysis6
Transmembranei91 – 111HelicalSequence analysisAdd BLAST21
Topological domaini112 – 282CytoplasmicSequence analysisAdd BLAST171
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Topological domaini304 – 312ExtracellularSequence analysis9
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Topological domaini334 – 815CytoplasmicSequence analysisAdd BLAST482
Transmembranei816 – 836HelicalSequence analysisAdd BLAST21
Topological domaini837 – 848ExtracellularSequence analysisAdd BLAST12
Transmembranei849 – 869HelicalSequence analysisAdd BLAST21
Topological domaini870 – 885CytoplasmicSequence analysisAdd BLAST16
Transmembranei886 – 906HelicalSequence analysisAdd BLAST21
Topological domaini907 – 943ExtracellularSequence analysisAdd BLAST37
Transmembranei944 – 964HelicalSequence analysisAdd BLAST21
Topological domaini965 – 991CytoplasmicSequence analysisAdd BLAST27
Transmembranei992 – 1012HelicalSequence analysisAdd BLAST21
Topological domaini1013 – 1021ExtracellularSequence analysis9
Transmembranei1022 – 1042HelicalSequence analysisAdd BLAST21
Topological domaini1043 – 1091CytoplasmicSequence analysisAdd BLAST49

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462421 – 1091Sodium transport ATPase 1Add BLAST1091

Proteomic databases

MaxQBiP13587
PaxDbiP13587
PRIDEiP13587

PTM databases

iPTMnetiP13587

Expressioni

Inductioni

By sodium ions. Induction at low salt concentrations (0.3 M) is mediated by the high-osmolarity glycerol (HOG)-MAP kinase pathway, a system activated by non-specific osmotic stress, and by the protein kinase A pathway. At high salt concentrations (0.8 M) is mediated by the protein phosphatase calcineurin, which is specifically activated by sodium ions.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi32095, 83 interactors
DIPiDIP-4493N
IntActiP13587, 4 interactors
STRINGi4932.YDR040C

Structurei

3D structure databases

ProteinModelPortaliP13587
SMRiP13587
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000144699
HOGENOMiHOG000265621
InParanoidiP13587
KOiK01536
OMAiWIIMATS
OrthoDBiEOG092C0DMA

Family and domain databases

CDDicd02086 P-type_ATPase_Na_ENA, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006414 P-type_ATPase_IID
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 2 hits
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01523 ATPase-IID_K-Na, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

P13587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEGTTKENN NAEFNAYHTL TAEEAAEFIG TSLTEGLTQD EFVHRLKTVG
60 70 80 90 100
ENTLGDDTKI DYKAMVLHQV CNAMIMVLLI SMIISFAMHD WITGGVISFV
110 120 130 140 150
IAVNVLIGLV QEYKATKTMN SLKNLSSPNA HVIRNGKSET INSKDVVPGD
160 170 180 190 200
ICLVKVGDTI PADLRLIETK NFDTDESLLT GESLPVSKDA NLVFGKEEET
210 220 230 240 250
SVGDRLNLAF SSSAVVKGRA KGIVIKTALN SEIGKIAKSL QGDSGLISRD
260 270 280 290 300
PSKSWLQNTW ISTKKVTGAF LGTNVGTPLH RKLSKLAVLL FWIAVLFAII
310 320 330 340 350
VMASQKFDVD KRVAIYAICV ALSMIPSSLV VVLTITMSVG AAVMVSRNVI
360 370 380 390 400
VRKLDSLEAL GAVNDICSDK TGTLTQGKML ARQIWIPRFG TITISNSDDP
410 420 430 440 450
FNPNEGNVSL IPRFSPYEYS HNEDGDVGIL QNFKDRLYEK DLPEDIDMDL
460 470 480 490 500
FQKWLETATL ANIATVFKDD ATDCWKAHGD PTEIAIQVFA TKMDLPHNAL
510 520 530 540 550
TGEKSTNQSN ENDQSSLSQH NEKPGSAQFE HIAEFPFDST VKRMSSVYYN
560 570 580 590 600
NHNETYNIYG KGAFESIISC CSSWYGKDGV KITPLTDCDV ETIRKNVYSL
610 620 630 640 650
SNEGLRVLGF ASKSFTKDQV NDDQLKNITS NRATAESDLV FLGLIGIYDP
660 670 680 690 700
PRNETAGAVK KFHQAGINVH MLTGDFVGTA KAIAQEVGIL PTNLYHYSQE
710 720 730 740 750
IVDSMVMTGS QFDGLSEEEV DDLPVLPLVI ARCSPQTKVR MIEALHRRKK
760 770 780 790 800
FCTMTGDGVN DSPSLKMANV GIAMGINGSD VSKEASDIVL SDDNFASILN
810 820 830 840 850
AVEEGRRMTD NIQKFVLQLL AENVAQALYL IIGLVFRDEN GKSVFPLSPV
860 870 880 890 900
EVLWIIVVTS CFPAMGLGLE KAAPDLMDRP PHDSEVGIFT WEVIIDTFAY
910 920 930 940 950
GIIMTGSCMA SFTGSLYGIN SGRLGHDCDG TYNSSCRDVY RSRSAAFATM
960 970 980 990 1000
TWCALILAWE VVDMRRSFFR MHPDTDSPVK EFFRSIWGNQ FLFWSIIFGF
1010 1020 1030 1040 1050
VSAFPVVYIP VINDKVFLHK PIGAEWGLAI AFTIAFWIGA ELYKCGKRRY
1060 1070 1080 1090
FKTQRAHNPE NDLESNNKRD PFEAYSTSTT IHTEVNIGIK Q
Length:1,091
Mass (Da):120,357
Last modified:January 1, 1990 - v1
Checksum:i6480DBCD92B555E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24069 Genomic DNA Translation: AAA65600.1
Z54075 Genomic DNA Translation: CAA90779.1
Z74336 Genomic DNA Translation: CAA98867.1
X58626 Genomic DNA Translation: CAA41479.1
J04186 Genomic DNA No translation available.
BK006938 Genomic DNA Translation: DAA11888.1
PIRiS05788 PWBYR2
RefSeqiNP_010325.1, NM_001180348.1

Genome annotation databases

EnsemblFungiiYDR040C; YDR040C; YDR040C
GeneIDi851610
KEGGisce:YDR040C

Similar proteinsi

Entry informationi

Entry nameiATN1_YEAST
AccessioniPrimary (citable) accession number: P13587
Secondary accession number(s): D6VS28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 18, 2018
This is version 188 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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