UniProtKB - P13514 (LSPA_KLEAK)
Protein
Lipoprotein signal peptidase
Gene
lspA
Organism
Klebsiella aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006) (Enterobacter aerogenes)
Status
Functioni
This protein specifically catalyzes the removal of signal peptides from prolipoproteins.
Catalytic activityi
- Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. EC:3.4.23.36
: lipoprotein biosynthesis (signal peptide cleavage) Pathwayi
This protein is involved in the pathway lipoprotein biosynthesis (signal peptide cleavage), which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (signal peptide cleavage) and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 114 | By similarity | 1 | |
Active sitei | 141 | By similarity | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Enzyme and pathway databases
BioCyci | EAER1028307:G1GYC-2217-MONOMER |
UniPathwayi | UPA00665 |
Protein family/group databases
MEROPSi | A08.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Lipoprotein signal peptidase (EC:3.4.23.36)Alternative name(s): Prolipoprotein signal peptidase Signal peptidase II Short name: SPase II |
Gene namesi | Name:lspA Synonyms:lsp Ordered Locus Names:EAE_10850 |
Organismi | Klebsiella aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006) (Enterobacter aerogenes) |
Taxonomic identifieri | 1028307 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane By similarity; Multi-pass membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 22 | HelicalSequence analysisAdd BLAST | 11 | |
Topological domaini | 23 – 69 | PeriplasmicSequence analysisAdd BLAST | 47 | |
Transmembranei | 70 – 88 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 89 – 95 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 96 – 113 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 114 – 138 | PeriplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 139 – 153 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 154 – 165 | CytoplasmicSequence analysisAdd BLAST | 12 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178781 | 1 – 165 | Lipoprotein signal peptidaseAdd BLAST | 165 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase A8 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0597, Bacteria |
HOGENOMi | CLU_083252_4_0_6 |
OMAi | MELWRIR |
Family and domain databases
HAMAPi | MF_00161, LspA, 1 hit |
InterProi | View protein in InterPro IPR001872, Peptidase_A8 |
PANTHERi | PTHR33695, PTHR33695, 1 hit |
Pfami | View protein in Pfam PF01252, Peptidase_A8, 1 hit |
PRINTSi | PR00781, LIPOSIGPTASE |
TIGRFAMsi | TIGR00077, lspA, 1 hit |
PROSITEi | View protein in PROSITE PS00855, SPASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P13514-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKSICSTGL RWLWVVVAVL IIDLGSKFLI LQNFALGETV SLFPSLNLHY
60 70 80 90 100
ARNYGAAFSF LADSGGWQRW FFAGIAVGIC VVLAVLMYRS KATQKLNNIA
110 120 130 140 150
YALIIGGALG NLFDRLWHGF VVDMIDFYVG DWHFATFNLA DSAICIGAAL
160
IVLEGFLPSS DKKTS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26713 Genomic DNA Translation: AAA24804.1 CP002824 Genomic DNA Translation: AEG97086.1 |
RefSeqi | WP_015368327.1, NC_015663.1 YP_004592365.1, NC_015663.1 |
Genome annotation databases
EnsemblBacteriai | AEG97086; AEG97086; EAE_10850 |
GeneIDi | 56719371 |
KEGGi | eae:EAE_10850 |
PATRICi | fig|1028307.3.peg.2162 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26713 Genomic DNA Translation: AAA24804.1 CP002824 Genomic DNA Translation: AEG97086.1 |
RefSeqi | WP_015368327.1, NC_015663.1 YP_004592365.1, NC_015663.1 |
3D structure databases
SMRi | P13514 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1028307.EAE_10850 |
Protein family/group databases
MEROPSi | A08.001 |
Genome annotation databases
EnsemblBacteriai | AEG97086; AEG97086; EAE_10850 |
GeneIDi | 56719371 |
KEGGi | eae:EAE_10850 |
PATRICi | fig|1028307.3.peg.2162 |
Phylogenomic databases
eggNOGi | COG0597, Bacteria |
HOGENOMi | CLU_083252_4_0_6 |
OMAi | MELWRIR |
Enzyme and pathway databases
UniPathwayi | UPA00665 |
BioCyci | EAER1028307:G1GYC-2217-MONOMER |
Family and domain databases
HAMAPi | MF_00161, LspA, 1 hit |
InterProi | View protein in InterPro IPR001872, Peptidase_A8 |
PANTHERi | PTHR33695, PTHR33695, 1 hit |
Pfami | View protein in Pfam PF01252, Peptidase_A8, 1 hit |
PRINTSi | PR00781, LIPOSIGPTASE |
TIGRFAMsi | TIGR00077, lspA, 1 hit |
PROSITEi | View protein in PROSITE PS00855, SPASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LSPA_KLEAK | |
Accessioni | P13514Primary (citable) accession number: P13514 Secondary accession number(s): G0E467 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Peptidase families
Classification of peptidase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families