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UniProtKB - P13482 (TREA_ECOLI)
Protein
Periplasmic trehalase
Gene
treA
Organism
Escherichia coli (strain K12)
Status
Functioni
Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system.
Catalytic activityi
- EC:3.2.1.28UniRule annotation2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 152 | SubstrateCurated | 1 | |
Binding sitei | 196 | SubstrateCurated | 1 | |
Binding sitei | 310 | Substrate; via carbonyl oxygenCurated | 1 | |
Active sitei | 312 | Proton donor/acceptor2 Publications | 1 | |
Active sitei | 496 | Proton donor/acceptor2 Publications | 1 | |
Binding sitei | 511 | SubstrateCurated | 1 |
GO - Molecular functioni
- alpha,alpha-trehalase activity Source: UniProtKB
GO - Biological processi
- cellular hyperosmotic response Source: EcoCyc
- cellular response to DNA damage stimulus Source: EcoliWiki
- trehalose catabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:TREHALAPERI-MONOMER |
BRENDAi | 3.2.1.28, 2026 |
Protein family/group databases
CAZyi | GH37, Glycoside Hydrolase Family 37 |
Names & Taxonomyi
Protein namesi | Recommended name: Periplasmic trehalaseUniRule annotation (EC:3.2.1.28UniRule annotation)Alternative name(s): Alpha,alpha-trehalaseUniRule annotation Alpha,alpha-trehalose glucohydrolaseUniRule annotation Short name: Tre37A |
Gene namesi | Name:treAUniRule annotation Synonyms:osmA Ordered Locus Names:b1197, JW1186 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | UniRule annotation1 PublicationAdd BLAST | 30 | |
ChainiPRO_0000012041 | 31 – 565 | Periplasmic trehalaseAdd BLAST | 535 |
Proteomic databases
jPOSTi | P13482 |
PaxDbi | P13482 |
PRIDEi | P13482 |
Expressioni
Inductioni
By growth at high osmolarity, is regulated by cAMP.
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260095, 135 interactors |
DIPi | DIP-11022N |
IntActi | P13482, 5 interactors |
STRINGi | 511145.b1197 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P13482 |
SMRi | P13482 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13482 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 159 – 160 | Substrate bindingCurated | 2 | |
Regioni | 205 – 207 | Substrate bindingCurated | 3 | |
Regioni | 277 – 279 | Substrate bindingCurated | 3 | |
Regioni | 538 – 565 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 544 – 565 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 37 family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1626, Bacteria |
HOGENOMi | CLU_006451_3_1_6 |
InParanoidi | P13482 |
OMAi | TNGVLIW |
PhylomeDBi | P13482 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
HAMAPi | MF_01060, Peripl_trehalase, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001661, Glyco_hydro_37 IPR018232, Glyco_hydro_37_CS IPR023720, Trehalase_periplasmic |
PANTHERi | PTHR23403, PTHR23403, 1 hit |
Pfami | View protein in Pfam PF01204, Trehalase, 1 hit |
PRINTSi | PR00744, GLHYDRLASE37 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00927, TREHALASE_1, 1 hit PS00928, TREHALASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P13482-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSPAPSRPQ KMALIPACIF LCFAALSVQA EETPVTPQPP DILLGPLFND
60 70 80 90 100
VQNAKLFPDQ KTFADAVPNS DPLMILADYR MQQNQSGFDL RHFVNVNFTL
110 120 130 140 150
PKEGEKYVPP EGQSLREHID GLWPVLTRST ENTEKWDSLL PLPEPYVVPG
160 170 180 190 200
GRFREVYYWD SYFTMLGLAE SGHWDKVADM VANFAHEIDT YGHIPNGNRS
210 220 230 240 250
YYLSRSQPPF FALMVELLAQ HEGDAALKQY LPQMQKEYAY WMDGVENLQA
260 270 280 290 300
GQQEKRVVKL QDGTLLNRYW DDRDTPRPES WVEDIATAKS NPNRPATEIY
310 320 330 340 350
RDLRSAAASG WDFSSRWMDN PQQLNTLRTT SIVPVDLNSL MFKMEKILAR
360 370 380 390 400
ASKAAGDNAM ANQYETLANA RQKGIEKYLW NDQQGWYADY DLKSHKVRNQ
410 420 430 440 450
LTAAALFPLY VNAAAKDRAN KMATATKTHL LQPGGLNTTS VKSGQQWDAP
460 470 480 490 500
NGWAPLQWVA TEGLQNYGQK EVAMDISWHF LTNVQHTYDR EKKLVEKYDV
510 520 530 540 550
STTGTGGGGG EYPLQDGFGW TNGVTLKMLD LICPKEQPCD NVPATRPTVK
560
SATTQPSTKE AQPTP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15868 Genomic DNA Translation: CAA33878.1 U00096 Genomic DNA Translation: AAC74281.1 AP009048 Genomic DNA Translation: BAA36054.1 |
PIRi | S04782 |
RefSeqi | NP_415715.1, NC_000913.3 WP_000841714.1, NZ_SSZK01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC74281; AAC74281; b1197 BAA36054; BAA36054; BAA36054 |
GeneIDi | 945757 |
KEGGi | ecj:JW1186 eco:b1197 |
PATRICi | fig|1411691.4.peg.1089 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15868 Genomic DNA Translation: CAA33878.1 U00096 Genomic DNA Translation: AAC74281.1 AP009048 Genomic DNA Translation: BAA36054.1 |
PIRi | S04782 |
RefSeqi | NP_415715.1, NC_000913.3 WP_000841714.1, NZ_SSZK01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JF4 | X-ray | 2.20 | A | 31-565 | [»] | |
2JG0 | X-ray | 1.50 | A | 31-565 | [»] | |
2JJB | X-ray | 1.90 | A/B/C/D | 31-565 | [»] | |
2WYN | X-ray | 2.10 | A/B/C/D | 31-565 | [»] | |
AlphaFoldDBi | P13482 | |||||
SMRi | P13482 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260095, 135 interactors |
DIPi | DIP-11022N |
IntActi | P13482, 5 interactors |
STRINGi | 511145.b1197 |
Protein family/group databases
CAZyi | GH37, Glycoside Hydrolase Family 37 |
Proteomic databases
jPOSTi | P13482 |
PaxDbi | P13482 |
PRIDEi | P13482 |
Genome annotation databases
EnsemblBacteriai | AAC74281; AAC74281; b1197 BAA36054; BAA36054; BAA36054 |
GeneIDi | 945757 |
KEGGi | ecj:JW1186 eco:b1197 |
PATRICi | fig|1411691.4.peg.1089 |
Organism-specific databases
EchoBASEi | EB1010 |
Phylogenomic databases
eggNOGi | COG1626, Bacteria |
HOGENOMi | CLU_006451_3_1_6 |
InParanoidi | P13482 |
OMAi | TNGVLIW |
PhylomeDBi | P13482 |
Enzyme and pathway databases
BioCyci | EcoCyc:TREHALAPERI-MONOMER |
BRENDAi | 3.2.1.28, 2026 |
Miscellaneous databases
EvolutionaryTracei | P13482 |
PROi | PR:P13482 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
HAMAPi | MF_01060, Peripl_trehalase, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001661, Glyco_hydro_37 IPR018232, Glyco_hydro_37_CS IPR023720, Trehalase_periplasmic |
PANTHERi | PTHR23403, PTHR23403, 1 hit |
Pfami | View protein in Pfam PF01204, Trehalase, 1 hit |
PRINTSi | PR00744, GLHYDRLASE37 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00927, TREHALASE_1, 1 hit PS00928, TREHALASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TREA_ECOLI | |
Accessioni | P13482Primary (citable) accession number: P13482 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families