UniProtKB - P13445 (RPOS_ECOLI)
Protein
RNA polymerase sigma factor RpoS
Gene
rpoS
Organism
Escherichia coli (strain K12)
Status
Functioni
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.UniRule annotation5 Publications
Protects stationary phase cells from killing induced by endoribonuclease MazF.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 288 – 307 | H-T-H motifUniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- bacterial-type RNA polymerase core enzyme binding Source: EcoCyc
- DNA binding Source: UniProtKB-UniRule
- DNA-binding transcription factor activity Source: InterPro
- sigma factor activity Source: EcoCyc
GO - Biological processi
Keywordsi
Molecular function | DNA-binding, Sigma factor |
Biological process | Stress response, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:RPOS-MONOMER MetaCyc:RPOS-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase sigma factor RpoSUniRule annotationAlternative name(s): Sigma SUniRule annotation Sigma-38UniRule annotation |
Gene namesi | Name:rpoSUniRule annotation Synonyms:appR, katF, nur, otsX, sigS Ordered Locus Names:b2741, JW5437 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Stationary phase cells lose resistance to killing by endoribonuclease MazF.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 173 | K → E: Eliminates RpoS proteolysis. Lack of interaction with RssB. 1 Publication | 1 | |
Mutagenesisi | 174 | E → T: 2-fold increase in RpoS half-life. Does not affect interaction with RssB. 1 Publication | 1 | |
Mutagenesisi | 177 | V → K: 3-fold increase in RpoS half-life. 1 Publication | 1 | |
Mutagenesisi | 178 | Y → L: Does not affect RpoS half-life. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:7256 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093970 | 1 – 330 | RNA polymerase sigma factor RpoSAdd BLAST | 330 |
Proteomic databases
jPOSTi | P13445 |
PaxDbi | P13445 |
PRIDEi | P13445 |
Expressioni
Inductioni
Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. Transcription is repressed by antitoxin MqsA (PubMed:21516113). mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease.6 Publications
Interactioni
Subunit structurei
Interacts with the RNA polymerase core enzyme and RssB.
UniRule annotation2 PublicationsBinary interactionsi
P13445
With | #Exp. | IntAct |
---|---|---|
rpoB [P0A8V2] | 5 | EBI-557581,EBI-544996 |
GO - Molecular functioni
- bacterial-type RNA polymerase core enzyme binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4262274, 308 interactors 851542, 1 interactor |
ComplexPortali | CPX-4883, DNA-directed RNA polymerase holoenzyme complex, SigmaS variant CPX-5024, rpoS-rssB sigma-antisigma complex |
DIPi | DIP-10777N |
IntActi | P13445, 10 interactors |
STRINGi | 511145.b2741 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P13445 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 89 | Sigma-70 factor domain-1Add BLAST | 34 | |
Regioni | 94 – 164 | Sigma-70 factor domain-2Add BLAST | 71 | |
Regioni | 174 – 249 | Sigma-70 factor domain-3Add BLAST | 76 | |
Regioni | 262 – 315 | Sigma-70 factor domain-4Add BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 118 – 121 | Interaction with polymerase core subunit RpoC | 4 |
Domaini
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).By similarity
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0568, Bacteria |
HOGENOMi | CLU_014793_3_5_6 |
InParanoidi | P13445 |
PhylomeDBi | P13445 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
HAMAPi | MF_00959, Sigma70_RpoS, 1 hit |
InterProi | View protein in InterPro IPR014284, RNA_pol_sigma-70_dom IPR000943, RNA_pol_sigma70 IPR009042, RNA_pol_sigma70_r1_2 IPR007627, RNA_pol_sigma70_r2 IPR007624, RNA_pol_sigma70_r3 IPR007630, RNA_pol_sigma70_r4 IPR013325, RNA_pol_sigma_r2 IPR013324, RNA_pol_sigma_r3/r4-like IPR012761, RNA_pol_sigma_RpoS IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00140, Sigma70_r1_2, 1 hit PF04542, Sigma70_r2, 1 hit PF04539, Sigma70_r3, 1 hit PF04545, Sigma70_r4, 1 hit |
PRINTSi | PR00046, SIGMA70FCT |
SUPFAMi | SSF88659, SSF88659, 2 hits SSF88946, SSF88946, 1 hit |
TIGRFAMsi | TIGR02394, rpoS_proteo, 1 hit TIGR02937, sigma70-ECF, 1 hit |
PROSITEi | View protein in PROSITE PS00715, SIGMA70_1, 1 hit PS00716, SIGMA70_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P13445-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA
60 70 80 90 100
TQRVLDATQL YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR
110 120 130 140 150
LVVKIARRYG NRGLALLDLI EEGNLGLIRA VEKFDPERGF RFSTYATWWI
160 170 180 190 200
RQTIERAIMN QTRTIRLPIH IVKELNVYLR TARELSHKLD HEPSAEEIAE
210 220 230 240 250
QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD EKENGPEDTT
260 270 280 290 300
QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
310 320 330
RVRQIQVEGL RRLREILQTQ GLNIEALFRE
Sequence cautioni
The sequence BAE76818 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
The sequence CAA34435 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA78692 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | F → L in CAA78692 (PubMed:1437569).Curated | 1 | |
Sequence conflicti | 29 | A → P in CAA34435 (PubMed:2690013).Curated | 1 | |
Sequence conflicti | 33 | Q → L in BAA02747 (PubMed:8475100).Curated | 1 | |
Sequence conflicti | 33 | Q → L in BAA21003 (PubMed:8208244).Curated | 1 | |
Sequence conflicti | 196 | E → V in CAA34435 (PubMed:2690013).Curated | 1 | |
Sequence conflicti | 328 – 330 | FRE → LPRVSKHLSERPVSSEAGFF CAQ in CAA34435 (PubMed:2690013).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16400 Genomic DNA Translation: CAA34435.1 Different initiation. D13548 Genomic DNA Translation: BAA02747.1 Z14969 Genomic DNA Translation: CAA78692.1 Different initiation. U29579 Genomic DNA Translation: AAA69251.1 U00096 Genomic DNA Translation: AAC75783.1 AP009048 Genomic DNA Translation: BAE76818.1 Sequence problems. D17549 Genomic DNA Translation: BAA21003.1 L07869 Unassigned DNA Translation: AAA17876.1 |
PIRi | A65055, RNECKF |
RefSeqi | NP_417221.1, NC_000913.3 WP_000081588.1, NZ_CP014272.1 |
Genome annotation databases
EnsemblBacteriai | AAC75783; AAC75783; b2741 BAE76818; BAE76818; BAE76818 |
GeneIDi | 947210 |
KEGGi | ecj:JW5437 eco:b2741 |
PATRICi | fig|511145.12.peg.2835 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16400 Genomic DNA Translation: CAA34435.1 Different initiation. D13548 Genomic DNA Translation: BAA02747.1 Z14969 Genomic DNA Translation: CAA78692.1 Different initiation. U29579 Genomic DNA Translation: AAA69251.1 U00096 Genomic DNA Translation: AAC75783.1 AP009048 Genomic DNA Translation: BAE76818.1 Sequence problems. D17549 Genomic DNA Translation: BAA21003.1 L07869 Unassigned DNA Translation: AAA17876.1 |
PIRi | A65055, RNECKF |
RefSeqi | NP_417221.1, NC_000913.3 WP_000081588.1, NZ_CP014272.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IPL | X-ray | 3.60 | F | 1-328 | [»] | |
5IPM | X-ray | 4.20 | F | 1-328 | [»] | |
5IPN | X-ray | 4.61 | F | 1-328 | [»] | |
6FI7 | NMR | - | A | 245-330 | [»] | |
6IDO | X-ray | 3.75 | A/B | 256-330 | [»] | |
6KJ6 | electron microscopy | 3.80 | F | 1-330 | [»] | |
6UTV | X-ray | 3.45 | FFF | 1-328 | [»] | |
6UTW | X-ray | 3.85 | FFF | 1-328 | [»] | |
6UTX | X-ray | 4.05 | FFF | 1-328 | [»] | |
6UTY | X-ray | 4.15 | FFF | 1-328 | [»] | |
6UTZ | X-ray | 3.80 | FFF | 1-328 | [»] | |
6UU0 | X-ray | 3.90 | FFF | 1-328 | [»] | |
6UU1 | X-ray | 4.10 | FFF | 1-328 | [»] | |
6UU2 | X-ray | 4.40 | FFF | 1-328 | [»] | |
6UU3 | X-ray | 4.00 | FFF | 1-328 | [»] | |
6UU4 | X-ray | 4.30 | FFF | 1-328 | [»] | |
6UU5 | X-ray | 5.40 | FFF | 1-328 | [»] | |
6UU6 | X-ray | 4.20 | FFF | 1-328 | [»] | |
6UU7 | X-ray | 4.40 | FFF | 1-328 | [»] | |
6UUA | X-ray | 4.00 | FFF | 1-328 | [»] | |
6UUB | X-ray | 3.96 | FFF | 1-328 | [»] | |
6UUC | X-ray | 4.10 | FFF | 1-328 | [»] | |
SMRi | P13445 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262274, 308 interactors 851542, 1 interactor |
ComplexPortali | CPX-4883, DNA-directed RNA polymerase holoenzyme complex, SigmaS variant CPX-5024, rpoS-rssB sigma-antisigma complex |
DIPi | DIP-10777N |
IntActi | P13445, 10 interactors |
STRINGi | 511145.b2741 |
Proteomic databases
jPOSTi | P13445 |
PaxDbi | P13445 |
PRIDEi | P13445 |
Genome annotation databases
EnsemblBacteriai | AAC75783; AAC75783; b2741 BAE76818; BAE76818; BAE76818 |
GeneIDi | 947210 |
KEGGi | ecj:JW5437 eco:b2741 |
PATRICi | fig|511145.12.peg.2835 |
Organism-specific databases
EchoBASEi | EB0505 |
Phylogenomic databases
eggNOGi | COG0568, Bacteria |
HOGENOMi | CLU_014793_3_5_6 |
InParanoidi | P13445 |
PhylomeDBi | P13445 |
Enzyme and pathway databases
BioCyci | EcoCyc:RPOS-MONOMER MetaCyc:RPOS-MONOMER |
Miscellaneous databases
PHI-basei | PHI:7256 |
PROi | PR:P13445 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
HAMAPi | MF_00959, Sigma70_RpoS, 1 hit |
InterProi | View protein in InterPro IPR014284, RNA_pol_sigma-70_dom IPR000943, RNA_pol_sigma70 IPR009042, RNA_pol_sigma70_r1_2 IPR007627, RNA_pol_sigma70_r2 IPR007624, RNA_pol_sigma70_r3 IPR007630, RNA_pol_sigma70_r4 IPR013325, RNA_pol_sigma_r2 IPR013324, RNA_pol_sigma_r3/r4-like IPR012761, RNA_pol_sigma_RpoS IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00140, Sigma70_r1_2, 1 hit PF04542, Sigma70_r2, 1 hit PF04539, Sigma70_r3, 1 hit PF04545, Sigma70_r4, 1 hit |
PRINTSi | PR00046, SIGMA70FCT |
SUPFAMi | SSF88659, SSF88659, 2 hits SSF88946, SSF88946, 1 hit |
TIGRFAMsi | TIGR02394, rpoS_proteo, 1 hit TIGR02937, sigma70-ECF, 1 hit |
PROSITEi | View protein in PROSITE PS00715, SIGMA70_1, 1 hit PS00716, SIGMA70_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPOS_ECOLI | |
Accessioni | P13445Primary (citable) accession number: P13445 Secondary accession number(s): Q2MA88, Q79EB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families