TATA-box-binding protein

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• chromatin binding Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "TATA-binding protein variants that bypass the requirement for Mot1 in vivo."
    Sprouse R.O., Wells M.N., Auble D.T.
    J. Biol. Chem. 284:4525-4535(2009) [PubMed] [Europe PMC] [Abstract]
• disordered domain specific binding Source: CAFA <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "The N-terminal regions of estrogen receptor alpha and beta are unstructured in vitro and show different TBP binding properties."
    Warnmark A., Wikstrom A., Wright A.P., Gustafsson J.A., Hard T.
    J. Biol. Chem. 276:45939-45944(2001) [PubMed] [Europe PMC] [Abstract]
• DNA binding, bending Source: SGDInferred from direct assayⁱ
  • "Transcription factor TFIID induces DNA bending upon binding to the TATA element."
    Horikoshi M., Bertuccioli C., Takada R., Wang J., Yamamoto T., Roeder R.G.
    Proc. Natl. Acad. Sci. U.S.A. 89:1060-1064(1992) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase I general transcription initiation factor binding Source: SGDInferred from direct assayⁱ
  • "The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor."
    Steffan J.S., Keys D.A., Dodd J.A., Nomura M.
    Genes Dev. 10:2551-2563(1996) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II activating transcription factor binding Source: SGDInferred from direct assayⁱ
  • "Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA."
    Klein J., Nolden M., Sanders S.L., Kirchner J., Weil P.A., Melcher K.
    J. Biol. Chem. 278:6779-6786(2003) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II core promoter sequence-specific DNA binding Source: SGDInferred from direct assayⁱ
  • Ref.3

    "Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box."
    Horikoshi M., Wang C.K., Fujii H., Cromlish J.A., Weil P.A., Roeder R.G.
    Nature 341:299-303(1989) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-34; 122-162 AND 198-233, CLEAVAGE OF INITIATOR METHIONINE.
• RNA polymerase II general transcription initiation factor activity Source: SGD
• RNA polymerase III general transcription initiation factor activity Source: SGD
• RNA polymerase III regulatory region DNA binding Source: SGDInferred from direct assayⁱ
  • "Cloning and functional characterization of the gene encoding the TFIIIB90 subunit of RNA polymerase III transcription factor TFIIIB."
    Roberts S., Miller S.J., Lane W.S., Lee S., Hahn S.
    J. Biol. Chem. 271:14903-14909(1996) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase I transcription regulator recruiting activity Source: SGDInferred from direct assayⁱ
  • "The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor."
    Steffan J.S., Keys D.A., Dodd J.A., Nomura M.
    Genes Dev. 10:2551-2563(1996) [PubMed] [Europe PMC] [Abstract]
• TFIIA-class transcription factor complex binding Source: CAFAInferred from physical interactionⁱ
  • Ref.17

    "Crystal structure of a yeast TFIIA/TBP/DNA complex."
    Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
    Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 61-240.
• transcription factor binding Source: CAFAInferred from physical interactionⁱ
  • "Structural and functional properties of the N transcriptional activation domain of thyroid transcription factor-1: similarities with the acidic activation domains."
    Tell G., Perrone L., Fabbro D., Pellizzari L., Pucillo C., De Felice M., Acquaviva R., Formisano S., Damante G.
    Biochem. J. 329:395-403(1998) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• nucleolar large rRNA transcription by RNA polymerase I Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells."
    Cormack B.P., Struhl K.
    Cell 69:685-696(1992) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase III preinitiation complex assembly Source: SGDInferred from direct assayⁱ
  • "The TATA-binding protein participates in TFIIIB assembly on tRNA genes."
    Huet J., Sentenac A.
    Nucleic Acids Res. 20:6451-6454(1992) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II preinitiation complex assembly Source: SGDInferred from mutant phenotypeⁱ
  • "Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly."
    Shen W.C., Bhaumik S.R., Causton H.C., Simon I., Zhu X., Jennings E.G., Wang T.H., Young R.A., Green M.R.
    EMBO J. 22:3395-3402(2003) [PubMed] [Europe PMC] [Abstract]
• transcription by RNA polymerase II Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• transposon integration Source: SGDInferred from direct assayⁱ
  • "The Brf and TATA-binding protein subunits of the RNA polymerase III transcription factor IIIB mediate position-specific integration of the gypsy-like element, Ty3."
    Yieh L., Kassavetis G., Geiduschek E.P., Sandmeyer S.B.
    J. Biol. Chem. 275:29800-29807(2000) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• disordered domain specific binding Source: CAFAInferred from physical interactionⁱ
  • "The N-terminal regions of estrogen receptor alpha and beta are unstructured in vitro and show different TBP binding properties."
    Warnmark A., Wikstrom A., Wright A.P., Gustafsson J.A., Hard T.
    J. Biol. Chem. 276:45939-45944(2001) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase I general transcription initiation factor binding Source: SGDInferred from direct assayⁱ
  • "The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor."
    Steffan J.S., Keys D.A., Dodd J.A., Nomura M.
    Genes Dev. 10:2551-2563(1996) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II activating transcription factor binding Source: SGDInferred from direct assayⁱ
  • "Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA."
    Klein J., Nolden M., Sanders S.L., Kirchner J., Weil P.A., Melcher K.
    J. Biol. Chem. 278:6779-6786(2003) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase I transcription regulator recruiting activity Source: SGDInferred from direct assayⁱ
  • "The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor."
    Steffan J.S., Keys D.A., Dodd J.A., Nomura M.
    Genes Dev. 10:2551-2563(1996) [PubMed] [Europe PMC] [Abstract]
• TFIIA-class transcription factor complex binding Source: CAFAInferred from physical interactionⁱ
  • Ref.17

    "Crystal structure of a yeast TFIIA/TBP/DNA complex."
    Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
    Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 61-240.
• transcription factor binding Source: CAFAInferred from physical interactionⁱ
  • "Structural and functional properties of the N transcriptional activation domain of thyroid transcription factor-1: similarities with the acidic activation domains."
    Tell G., Perrone L., Fabbro D., Pellizzari L., Pucillo C., De Felice M., Acquaviva R., Formisano S., Damante G.
    Biochem. J. 329:395-403(1998) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA 
        60         70         80         90        100
PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA 
       110        120        130        140        150
AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA 
       160        170        180        190        200
KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP 
       210        220        230        240
KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM            

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families
3. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
4. Yeast chromosome V
   Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names