UniProtKB - P13393 (TBP_YEAST)
TATA-box-binding protein
SPT15
Functioni
General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.
3 PublicationsGO - Molecular functioni
- chromatin binding Source: SGD
- disordered domain specific binding Source: CAFA
- DNA binding, bending Source: SGD
- DNA-binding transcription factor binding Source: CAFA
- general transcription initiation factor activity Source: GO_Central
- RNA polymerase I general transcription initiation factor binding Source: SGD
- RNA polymerase II core promoter sequence-specific DNA binding Source: SGD
- RNA polymerase II general transcription initiation factor activity Source: GO_Central
- RNA polymerase III general transcription initiation factor activity Source: SGD
- RNA polymerase III transcription regulatory region sequence-specific DNA binding Source: SGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: SGD
- TFIIA-class transcription factor complex binding Source: CAFA
GO - Biological processi
- DNA-templated transcription, initiation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: ComplexPortal
- nucleolar large rRNA transcription by RNA polymerase I Source: SGD
- positive regulation of transcription by RNA polymerase II Source: ComplexPortal
- regulation of transcription by RNA polymerase III Source: ComplexPortal
- RNA polymerase III preinitiation complex assembly Source: SGD
- RNA polymerase II preinitiation complex assembly Source: SGD
- RNA polymerase I preinitiation complex assembly Source: SGD
- transposon integration Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-73772, RNA Polymerase I Promoter Escape R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-76061, RNA Polymerase III Transcription Initiation From Type 1 Promoter R-SCE-76066, RNA Polymerase III Transcription Initiation From Type 2 Promoter R-SCE-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: TATA-box-binding proteinAlternative name(s): TATA sequence-binding protein Short name: TBP TATA-binding factor TATA-box factor Transcription factor D Transcription initiation factor TFIID TBP subunit |
Gene namesi | Name:SPT15 Synonyms:BTF1, TBP1 Ordered Locus Names:YER148W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000950, SPT15 |
VEuPathDBi | FungiDB:YER148W |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: SGD
- transcription factor TFIIA complex Source: CAFA
- transcription factor TFIID complex Source: SGD
Other locations
- protein-containing complex Source: CAFA
- transcription factor TFIIIB complex Source: SGD
- transcription regulator complex Source: ComplexPortal
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000153990 | 2 – 240 | TATA-box-binding proteinAdd BLAST | 239 |
Proteomic databases
MaxQBi | P13393 |
PaxDbi | P13393 |
PRIDEi | P13393 |
PTM databases
iPTMneti | P13393 |
Interactioni
Subunit structurei
Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.
Interacts with TFC8.
2 PublicationsBinary interactionsi
P13393
GO - Molecular functioni
- disordered domain specific binding Source: CAFA
- DNA-binding transcription factor binding Source: CAFA
- RNA polymerase I general transcription initiation factor binding Source: SGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: SGD
- TFIIA-class transcription factor complex binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 36897, 318 interactors |
ComplexPortali | CPX-1141, MOT1-TBP transcription regulation complex CPX-1143, Transcription factor TFIIIB complex CPX-1642, General transcription factor complex TFIID |
DIPi | DIP-44N |
IntActi | P13393, 74 interactors |
MINTi | P13393 |
STRINGi | 4932.YER148W |
Miscellaneous databases
RNActi | P13393, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P13393 |
SMRi | P13393 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13393 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 67 – 143 | 1Add BLAST | 77 | |
Repeati | 157 – 234 | 2Add BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 61 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 21 – 40 | Polar residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3302, Eukaryota |
GeneTreei | ENSGT00940000159561 |
HOGENOMi | CLU_060161_4_2_1 |
InParanoidi | P13393 |
OMAi | NCEYEPE |
Family and domain databases
CDDi | cd04516, TBP_eukaryotes, 1 hit |
Gene3Di | 3.30.310.10, 2 hits |
HAMAPi | MF_00408, TATA_bind_prot_arch, 1 hit |
InterProi | View protein in InterPro IPR000814, TBP IPR030491, TBP_CS IPR012295, TBP_dom_sf IPR033710, TBP_eukaryotic |
PANTHERi | PTHR10126, PTHR10126, 1 hit |
Pfami | View protein in Pfam PF00352, TBP, 2 hits |
PRINTSi | PR00686, TIFACTORIID |
PROSITEi | View protein in PROSITE PS00351, TFIID, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA
60 70 80 90 100
PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA
110 120 130 140 150
AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA
160 170 180 190 200
KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP
210 220 230 240
KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | A → T in AAA35146 (PubMed:2682626).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27135 Genomic DNA Translation: AAA35147.1 X16860 Genomic DNA Translation: CAA34751.1 M26403 Genomic DNA Translation: AAA35146.1 M29459 Genomic DNA Translation: AAA34458.1 U18917 Genomic DNA Translation: AAB64675.1 BK006939 Genomic DNA Translation: DAA07809.1 |
PIRi | A30366 |
RefSeqi | NP_011075.3, NM_001179038.3 |
Genome annotation databases
EnsemblFungii | YER148W_mRNA; YER148W; YER148W |
GeneIDi | 856891 |
KEGGi | sce:YER148W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27135 Genomic DNA Translation: AAA35147.1 X16860 Genomic DNA Translation: CAA34751.1 M26403 Genomic DNA Translation: AAA35146.1 M29459 Genomic DNA Translation: AAA34458.1 U18917 Genomic DNA Translation: AAB64675.1 BK006939 Genomic DNA Translation: DAA07809.1 |
PIRi | A30366 |
RefSeqi | NP_011075.3, NM_001179038.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NGM | X-ray | 2.95 | A/E/I/M | 61-240 | [»] | |
1NH2 | X-ray | 1.90 | A | 61-240 | [»] | |
1RM1 | X-ray | 2.50 | A | 1-240 | [»] | |
1TBA | NMR | - | B | 61-240 | [»] | |
1TBP | X-ray | 2.60 | A/B | 62-240 | [»] | |
1YTB | X-ray | 1.80 | A/B | 61-240 | [»] | |
1YTF | X-ray | 2.50 | A | 61-240 | [»] | |
4B0A | X-ray | 1.97 | A | 61-240 | [»] | |
4V1N | electron microscopy | 7.80 | O | 61-240 | [»] | |
4V1O | electron microscopy | 9.70 | O | 61-240 | [»] | |
5FMF | electron microscopy | 6.00 | Q | 61-240 | [»] | |
5FYW | electron microscopy | 4.35 | O | 1-240 | [»] | |
5FZ5 | electron microscopy | 8.80 | O | 1-240 | [»] | |
5OQJ | electron microscopy | 4.70 | O | 1-240 | [»] | |
5OQM | electron microscopy | 5.80 | O | 1-240 | [»] | |
5SVA | electron microscopy | 15.30 | j | 1-240 | [»] | |
6CNB | electron microscopy | 4.10 | R | 61-240 | [»] | |
6CNC | electron microscopy | 4.10 | R | 61-240 | [»] | |
6CND | electron microscopy | 4.80 | R | 61-240 | [»] | |
6CNF | electron microscopy | 4.50 | R | 61-240 | [»] | |
6E16 | X-ray | 2.40 | A | 61-240 | [»] | |
6E24 | X-ray | 3.00 | A | 61-240 | [»] | |
6EU0 | electron microscopy | 4.00 | Y | 1-240 | [»] | |
6F40 | electron microscopy | 3.70 | U | 1-240 | [»] | |
6F41 | electron microscopy | 4.30 | U | 1-240 | [»] | |
6F42 | electron microscopy | 5.50 | U | 1-240 | [»] | |
6F44 | electron microscopy | 4.20 | U | 1-240 | [»] | |
6GYK | electron microscopy | 5.10 | O | 1-240 | [»] | |
6GYL | electron microscopy | 4.80 | O | 1-240 | [»] | |
6GYM | electron microscopy | 6.70 | O | 1-240 | [»] | |
6TBM | electron microscopy | 20.00 | M | 1-240 | [»] | |
7O4I | electron microscopy | 3.20 | O | 1-240 | [»] | |
7O4J | electron microscopy | 2.90 | O | 1-240 | [»] | |
7O72 | electron microscopy | 3.40 | O | 1-240 | [»] | |
7O73 | electron microscopy | 3.40 | O | 1-240 | [»] | |
7O75 | electron microscopy | 3.20 | O | 1-240 | [»] | |
BMRBi | P13393 | |||||
SMRi | P13393 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36897, 318 interactors |
ComplexPortali | CPX-1141, MOT1-TBP transcription regulation complex CPX-1143, Transcription factor TFIIIB complex CPX-1642, General transcription factor complex TFIID |
DIPi | DIP-44N |
IntActi | P13393, 74 interactors |
MINTi | P13393 |
STRINGi | 4932.YER148W |
PTM databases
iPTMneti | P13393 |
Proteomic databases
MaxQBi | P13393 |
PaxDbi | P13393 |
PRIDEi | P13393 |
Genome annotation databases
EnsemblFungii | YER148W_mRNA; YER148W; YER148W |
GeneIDi | 856891 |
KEGGi | sce:YER148W |
Organism-specific databases
SGDi | S000000950, SPT15 |
VEuPathDBi | FungiDB:YER148W |
Phylogenomic databases
eggNOGi | KOG3302, Eukaryota |
GeneTreei | ENSGT00940000159561 |
HOGENOMi | CLU_060161_4_2_1 |
InParanoidi | P13393 |
OMAi | NCEYEPE |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-73772, RNA Polymerase I Promoter Escape R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-76061, RNA Polymerase III Transcription Initiation From Type 1 Promoter R-SCE-76066, RNA Polymerase III Transcription Initiation From Type 2 Promoter R-SCE-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
EvolutionaryTracei | P13393 |
PROi | PR:P13393 |
RNActi | P13393, protein |
Family and domain databases
CDDi | cd04516, TBP_eukaryotes, 1 hit |
Gene3Di | 3.30.310.10, 2 hits |
HAMAPi | MF_00408, TATA_bind_prot_arch, 1 hit |
InterProi | View protein in InterPro IPR000814, TBP IPR030491, TBP_CS IPR012295, TBP_dom_sf IPR033710, TBP_eukaryotic |
PANTHERi | PTHR10126, PTHR10126, 1 hit |
Pfami | View protein in Pfam PF00352, TBP, 2 hits |
PRINTSi | PR00686, TIFACTORIID |
PROSITEi | View protein in PROSITE PS00351, TFIID, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | TBP_YEAST | |
Accessioni | P13393Primary (citable) accession number: P13393 Secondary accession number(s): D3DM55 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 220 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families