UniProtKB - P13385 (TDGF1_HUMAN)
Protein
Teratocarcinoma-derived growth factor 1
Gene
TDGF1
Organism
Homo sapiens (Human)
Status
Functioni
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).2 Publications
GO - Molecular functioni
- activin receptor binding Source: GO_Central
- growth factor activity Source: UniProtKB
- nodal binding Source: GO_Central
- signaling receptor binding Source: UniProtKB
GO - Biological processi
- activation of MAPK activity Source: UniProtKB
- anatomical structure development Source: GO_Central
- anterior/posterior axis specification, embryo Source: UniProtKB
- anterior/posterior pattern specification Source: GO_Central
- blood vessel development Source: GO_Central
- cell differentiation Source: UniProtKB
- cell migration involved in sprouting angiogenesis Source: UniProtKB
- cellular response to epidermal growth factor stimulus Source: UniProtKB
- cellular response to fibroblast growth factor stimulus Source: UniProtKB
- cellular response to hepatocyte growth factor stimulus Source: UniProtKB
- cellular response to interferon-gamma Source: UniProtKB
- cellular response to interleukin-6 Source: UniProtKB
- cellular response to tumor necrosis factor Source: UniProtKB
- determination of left/right symmetry Source: GO_Central
- embryo development ending in birth or egg hatching Source: UniProtKB
- heart development Source: UniProtKB
- mammary gland development Source: UniProtKB
- morphogenesis of a branching structure Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- nodal signaling pathway Source: GO_Central
- peptidyl-serine phosphorylation Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- positive regulation of endothelial cell migration Source: UniProtKB
- positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
- regulation of signal transduction Source: UniProtKB
- somatic stem cell population maintenance Source: Reactome
Keywordsi
Molecular function | Growth factor |
Enzyme and pathway databases
PathwayCommonsi | P13385 |
Reactomei | R-HSA-1181150, Signaling by NODAL R-HSA-1433617, Regulation of signaling by NODAL R-HSA-2892247, POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation |
SIGNORi | P13385 |
Names & Taxonomyi
Protein namesi | Recommended name: Teratocarcinoma-derived growth factor 1Alternative name(s): Cripto-1 growth factor Short name: CRGF Epidermal growth factor-like cripto protein CR1 |
Gene namesi | Name:TDGF1 Synonyms:CRIPTO |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11701, TDGF1 |
MIMi | 187395, gene+phenotype |
neXtProti | NX_P13385 |
VEuPathDBi | HostDB:ENSG00000241186.7 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Plasma membrane
- Cell membrane 2 Publications; GPI-anchor 2 Publications
Note: Released from the cell membrane by GPI cleavage.1 Publication
Extracellular region or secreted
- extracellular region Source: GO_Central
- extracellular space Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- extrinsic component of plasma membrane Source: UniProtKB
- plasma membrane Source: Reactome
Other locations
- anchored component of membrane Source: UniProtKB-KW
- cell surface Source: UniProtKB
- membrane raft Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 188 | Y → YVVVVV: Alters the localization and decreases the biological activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6997 |
GeneReviewsi | TDGF1 |
MalaCardsi | TDGF1 |
MIMi | 187395, gene+phenotype |
OpenTargetsi | ENSG00000241186 |
Orphaneti | 93925, Alobar holoprosencephaly 93924, Lobar holoprosencephaly 280200, Microform holoprosencephaly 93926, Midline interhemispheric variant of holoprosencephaly 220386, Semilobar holoprosencephaly 280195, Septopreoptic holoprosencephaly |
PharmGKBi | PA36420 |
Miscellaneous databases
Pharosi | P13385, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3713025 |
Genetic variation databases
BioMutai | TDGF1 |
DMDMi | 117473 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | 1 PublicationAdd BLAST | 30 | |
ChainiPRO_0000007503 | 31 – 150 | Teratocarcinoma-derived growth factor 1Add BLAST | 120 | |
PropeptideiPRO_0000395410 | 151 – 188 | Removed in mature formAdd BLAST | 38 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 82 ↔ 89 | PROSITE-ProRule annotation | ||
Disulfide bondi | 83 ↔ 95 | PROSITE-ProRule annotation | ||
Disulfide bondi | 97 ↔ 106 | PROSITE-ProRule annotation | ||
Disulfide bondi | 115 ↔ 133 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 128 ↔ 149 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 131 ↔ 140 | PROSITE-ProRule annotation1 Publication | ||
Lipidationi | 150 | GPI-anchor amidated aspartate1 Publication | 1 |
Post-translational modificationi
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing TDGF1 as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
EPDi | P13385 |
jPOSTi | P13385 |
MassIVEi | P13385 |
PaxDbi | P13385 |
PeptideAtlasi | P13385 |
PRIDEi | P13385 |
ProteomicsDBi | 52909 |
PTM databases
GlyGeni | P13385, 1 site |
Expressioni
Tissue specificityi
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.1 Publication
Gene expression databases
Bgeei | ENSG00000241186, Expressed in cortex of kidney and 97 other tissues |
ExpressionAtlasi | P13385, baseline and differential |
Genevisiblei | P13385, HS |
Organism-specific databases
HPAi | ENSG00000241186, Tissue enhanced (kidney, lymphoid tissue, ovary) |
Interactioni
Subunit structurei
Interacts with the activin type-1 receptor ACVR1B.
1 PublicationGO - Molecular functioni
- activin receptor binding Source: GO_Central
- growth factor activity Source: UniProtKB
- nodal binding Source: GO_Central
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 112856, 43 interactors |
IntActi | P13385, 28 interactors |
MINTi | P13385 |
STRINGi | 9606.ENSP00000296145 |
Miscellaneous databases
RNActi | P13385, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 78 – 107 | EGF-likePROSITE-ProRule annotationAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.Curated
Keywords - Domaini
EGF-like domain, SignalPhylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000159076 |
InParanoidi | P13385 |
OMAi | PMGIQNS |
OrthoDBi | 1387592at2759 |
PhylomeDBi | P13385 |
TreeFami | TF333187 |
Family and domain databases
InterProi | View protein in InterPro IPR017047, Cripto_growth_factor IPR019011, Cryptic/Cripto_CFC-dom IPR000742, EGF-like_dom |
Pfami | View protein in Pfam PF09443, CFC, 1 hit |
PIRSFi | PIRSF036301, Cripto_growth_factor, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS50026, EGF_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P13385-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDCRKMARFS YSVIWIMAIS KVFELGLVAG LGHQEFARPS RGYLAFRDDS
60 70 80 90 100
IWPQEEPAIR PRSSQRVPPM GIQHSKELNR TCCLNGGTCM LGSFCACPPS
110 120 130 140 150
FYGRNCEHDV RKENCGSVPH DTWLPKKCSL CKCWHGQLRC FPQAFLPGCD
160 170 180
GLVMDEHLVA SRTPELPPSA RTTTFMLVGI CLSIQSYY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF5H1T8 | F5H1T8_HUMAN | Growth factor | TDGF1 | 172 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024262 | 22 | V → A2 PublicationsCorresponds to variant dbSNP:rs11130097Ensembl. | 1 | |
Natural variantiVAR_021903 | 43 | Y → D. Corresponds to variant dbSNP:rs2293025Ensembl. | 1 | |
Natural variantiVAR_048975 | 111 | R → G. Corresponds to variant dbSNP:rs34501971EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14253 mRNA Translation: CAA32467.1 M96955 Genomic DNA Translation: AAA61134.1 AC104304 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64773.1 BC022393 mRNA Translation: AAH22393.1 BC067844 mRNA Translation: AAH67844.1 |
CCDSi | CCDS2742.1 |
PIRi | B39787, A30362 |
RefSeqi | NP_001167607.1, NM_001174136.1 NP_003203.1, NM_003212.3 |
Genome annotation databases
Ensembli | ENST00000296145; ENSP00000296145; ENSG00000241186 |
GeneIDi | 6997 |
KEGGi | hsa:6997 |
UCSCi | uc003cpv.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14253 mRNA Translation: CAA32467.1 M96955 Genomic DNA Translation: AAA61134.1 AC104304 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64773.1 BC022393 mRNA Translation: AAH22393.1 BC067844 mRNA Translation: AAH67844.1 |
CCDSi | CCDS2742.1 |
PIRi | B39787, A30362 |
RefSeqi | NP_001167607.1, NM_001174136.1 NP_003203.1, NM_003212.3 |
3D structure databases
SMRi | P13385 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112856, 43 interactors |
IntActi | P13385, 28 interactors |
MINTi | P13385 |
STRINGi | 9606.ENSP00000296145 |
Chemistry databases
ChEMBLi | CHEMBL3713025 |
PTM databases
GlyGeni | P13385, 1 site |
Genetic variation databases
BioMutai | TDGF1 |
DMDMi | 117473 |
Proteomic databases
EPDi | P13385 |
jPOSTi | P13385 |
MassIVEi | P13385 |
PaxDbi | P13385 |
PeptideAtlasi | P13385 |
PRIDEi | P13385 |
ProteomicsDBi | 52909 |
Protocols and materials databases
Antibodypediai | 35134, 651 antibodies |
DNASUi | 6997 |
Genome annotation databases
Ensembli | ENST00000296145; ENSP00000296145; ENSG00000241186 |
GeneIDi | 6997 |
KEGGi | hsa:6997 |
UCSCi | uc003cpv.4, human |
Organism-specific databases
CTDi | 6997 |
DisGeNETi | 6997 |
GeneCardsi | TDGF1 |
GeneReviewsi | TDGF1 |
HGNCi | HGNC:11701, TDGF1 |
HPAi | ENSG00000241186, Tissue enhanced (kidney, lymphoid tissue, ovary) |
MalaCardsi | TDGF1 |
MIMi | 187395, gene+phenotype |
neXtProti | NX_P13385 |
OpenTargetsi | ENSG00000241186 |
Orphaneti | 93925, Alobar holoprosencephaly 93924, Lobar holoprosencephaly 280200, Microform holoprosencephaly 93926, Midline interhemispheric variant of holoprosencephaly 220386, Semilobar holoprosencephaly 280195, Septopreoptic holoprosencephaly |
PharmGKBi | PA36420 |
VEuPathDBi | HostDB:ENSG00000241186.7 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000159076 |
InParanoidi | P13385 |
OMAi | PMGIQNS |
OrthoDBi | 1387592at2759 |
PhylomeDBi | P13385 |
TreeFami | TF333187 |
Enzyme and pathway databases
PathwayCommonsi | P13385 |
Reactomei | R-HSA-1181150, Signaling by NODAL R-HSA-1433617, Regulation of signaling by NODAL R-HSA-2892247, POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation |
SIGNORi | P13385 |
Miscellaneous databases
BioGRID-ORCSi | 6997, 396 hits in 957 CRISPR screens |
ChiTaRSi | TDGF1, human |
GeneWikii | Teratocarcinoma-derived_growth_factor_1 |
GenomeRNAii | 6997 |
Pharosi | P13385, Tbio |
PROi | PR:P13385 |
RNActi | P13385, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000241186, Expressed in cortex of kidney and 97 other tissues |
ExpressionAtlasi | P13385, baseline and differential |
Genevisiblei | P13385, HS |
Family and domain databases
InterProi | View protein in InterPro IPR017047, Cripto_growth_factor IPR019011, Cryptic/Cripto_CFC-dom IPR000742, EGF-like_dom |
Pfami | View protein in Pfam PF09443, CFC, 1 hit |
PIRSFi | PIRSF036301, Cripto_growth_factor, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS50026, EGF_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDGF1_HUMAN | |
Accessioni | P13385Primary (citable) accession number: P13385 Secondary accession number(s): Q8TCC1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | April 7, 2021 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families