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Protein

Antiholin

Gene

rI

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in lysis inhibition. Interacts with and inhibits the holin thereby delaying the host cell lysis timing. Lysis inhibition is imposed when a cell infected by a T4-like virus is superinfected by new incoming viruses. This is probably due to the capsid content of the new phage being released in the periplasm because of blockage of entry due to superinfection exclusion. These periplasmically released viral proteins must somehow activate the periplasmic antiholin, which in turn will block the holin multimerization.UniRule annotation3 Publications

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • negative regulation of holin activity Source: CACAO

Keywordsi

Biological processCytolysis, Host cell lysis by virus, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
AntiholinUniRule annotation
Alternative name(s):
Protein rI
Gene namesi
Name:rI
Synonyms:58.6, rIA, tk.-2
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 24Helical; Signal-anchor for type II membrane proteinUniRule annotation1 PublicationAdd BLAST24
Topological domaini25 – 97PeriplasmicUniRule annotation2 PublicationsAdd BLAST73

GO - Cellular componenti

  • host cell plasma membrane Source: CACAO
  • integral component of membrane Source: UniProtKB-UniRule

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69C → S: Complete loss of lysis. 1 Publication1
Mutagenesisi75C → S: Complete loss of lysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000033461 – 97AntiholinAdd BLAST97

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 75UniRule annotation1 Publication

Post-translational modificationi

Disulfide bond is required for functionality.UniRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Interacts (via C-terminus) with holin (via C-terminus); this interaction blocks the holin homomultimerization and delays the host cell lysis.UniRule annotation1 Publication

Family & Domainsi

Domaini

The transmembrane signal-anchor domain can escape spontaneously from the cell inner membrane. It is called SAR for signal-anchor-release for that reason and explains why the antiholin is an extremly instable protein. Superinfection might stabilize it so that it can inhibit holin multimerization and delay lysis.UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the T4likevirus antiholin family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090001EV

Family and domain databases

HAMAPiMF_04105 ANTIH_T4, 1 hit
InterProiView protein in InterPro
IPR034696 RI_T4

Sequencei

Sequence statusi: Complete.

P13304-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALKATALFA MLGLSFVLSP SIEANVDPHF DKFMESGIRH VYMLFENKSV
60 70 80 90
ESSEQFYSFM RTTYKNDPCS SDFECIERGA EMAQSYARIM NIKLETE
Length:97
Mass (Da):11,125
Last modified:January 1, 1990 - v1
Checksum:iEA0EBE97B91265D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04567 Genomic DNA Translation: CAA28236.1
U76612 Genomic DNA Translation: AAB26962.1
AF158101 Genomic DNA Translation: AAD42646.1
RefSeqiNP_049717.1, NC_000866.4

Genome annotation databases

GeneIDi1258591
KEGGivg:1258591

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04567 Genomic DNA Translation: CAA28236.1
U76612 Genomic DNA Translation: AAB26962.1
AF158101 Genomic DNA Translation: AAD42646.1
RefSeqiNP_049717.1, NC_000866.4

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258591
KEGGivg:1258591

Phylogenomic databases

OrthoDBiVOG090001EV

Family and domain databases

HAMAPiMF_04105 ANTIH_T4, 1 hit
InterProiView protein in InterPro
IPR034696 RI_T4
ProtoNetiSearch...

Entry informationi

Entry nameiANTIH_BPT4
AccessioniPrimary (citable) accession number: P13304
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 10, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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