UniProtKB - P13286 (PHKG1_RAT)
Protein
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
Gene
Phkg1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).By similarity
Catalytic activityi
- 2 ATP + phosphorylase b = 2 ADP + phosphorylase a. EC:2.7.11.19
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 49 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 150 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 26 – 34 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: RGD
- calmodulin binding Source: UniProtKB-KW
- enzyme binding Source: RGD
- phosphorylase kinase activity Source: RGD
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
- tau-protein kinase activity Source: UniProtKB-EC
GO - Biological processi
- glycogen biosynthetic process Source: InterPro
- glycogen metabolic process Source: RGD
- protein phosphorylation Source: RGD
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.19, 5301 |
Reactomei | R-RNO-70221, Glycogen breakdown (glycogenolysis) |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Phkg1 Synonyms:Phkg |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3325, Phkg1 |
Subcellular locationi
Other locations
- phosphorylase kinase complex Source: RGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086511 | 1 – 388 | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformAdd BLAST | 388 |
Proteomic databases
PaxDbi | P13286 |
PRIDEi | P13286 |
PTM databases
iPTMneti | P13286 |
PhosphoSitePlusi | P13286 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000000920, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | P13286, RN |
Interactioni
Subunit structurei
Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- enzyme binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248008, 1 interactor |
STRINGi | 10116.ENSRNOP00000001222 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 288 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 269 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 303 – 327 | Calmodulin-binding (domain-N)Add BLAST | 25 | |
Regioni | 343 – 367 | Calmodulin-binding (domain-C)Add BLAST | 25 |
Domaini
The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0599, Eukaryota |
GeneTreei | ENSGT00940000158139 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | P13286 |
OMAi | YQYRRAK |
OrthoDBi | 330091at2759 |
PhylomeDBi | P13286 |
TreeFami | TF320349 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR002291, Phosph_kin_gamma IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01049, PHOSPHBKNASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P13286-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRDEALPDS HSAQNFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI
60 70 80 90 100
IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF
110 120 130 140 150
FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVVCT LHKLNIVHRD
160 170 180 190 200
LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM
210 220 230 240 250
DEGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG
260 270 280 290 300
SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS
310 320 330 340 350
PRGKFKVICL TVLASVRIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY
360 370 380
AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07320 mRNA Translation: CAA30280.1 |
PIRi | S00731 |
RefSeqi | NP_113761.1, NM_031573.1 |
Genome annotation databases
Ensembli | ENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920 |
GeneIDi | 29353 |
KEGGi | rno:29353 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07320 mRNA Translation: CAA30280.1 |
PIRi | S00731 |
RefSeqi | NP_113761.1, NM_031573.1 |
3D structure databases
SMRi | P13286 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248008, 1 interactor |
STRINGi | 10116.ENSRNOP00000001222 |
PTM databases
iPTMneti | P13286 |
PhosphoSitePlusi | P13286 |
Proteomic databases
PaxDbi | P13286 |
PRIDEi | P13286 |
Genome annotation databases
Ensembli | ENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920 |
GeneIDi | 29353 |
KEGGi | rno:29353 |
Organism-specific databases
CTDi | 5260 |
RGDi | 3325, Phkg1 |
Phylogenomic databases
eggNOGi | KOG0599, Eukaryota |
GeneTreei | ENSGT00940000158139 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | P13286 |
OMAi | YQYRRAK |
OrthoDBi | 330091at2759 |
PhylomeDBi | P13286 |
TreeFami | TF320349 |
Enzyme and pathway databases
BRENDAi | 2.7.11.19, 5301 |
Reactomei | R-RNO-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
PROi | PR:P13286 |
Gene expression databases
Bgeei | ENSRNOG00000000920, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | P13286, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR002291, Phosph_kin_gamma IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01049, PHOSPHBKNASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHKG1_RAT | |
Accessioni | P13286Primary (citable) accession number: P13286 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families