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Protein

Gamma-interferon-inducible lysosomal thiol reductase

Gene

IFI30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).By similarity

Miscellaneous

Both precursor form and mature form have thiol reductase activity.

Kineticsi

Kinetic parameters shown are for mature enzyme.
  1. KM=1200 µM for F(ab')2 fragment when denatured1 Publication
  2. KM=10 µM for F(ab')2 fragment1 Publication

    pH dependencei

    Optimum pH is 4-5.1 Publication

    GO - Molecular functioni

    • oxidoreductase activity, acting on a sulfur group of donors Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processImmunity

    Enzyme and pathway databases

    ReactomeiR-HSA-2132295 MHC class II antigen presentation
    R-HSA-877300 Interferon gamma signaling

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-interferon-inducible lysosomal thiol reductase (EC:1.8.-.-2 Publications)
    Alternative name(s):
    Gamma-interferon-inducible protein IP-30
    Legumaturain
    Gene namesi
    Name:IFI30
    Synonyms:GILT, IP30
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 19

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000216490.3
    HGNCiHGNC:5398 IFI30
    MIMi604664 gene
    neXtProtiNX_P13284

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Lysosome, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi72C → S: Abolishes reducing activity, does not affect dimerization. Abolishes reducing activity; when associated with S-75. 2 Publications1
    Mutagenesisi75C → S: Abolishes reducing activity, does not affect dimerization. Abolishes reducing activity; when associated with S-72. 2 Publications1

    Organism-specific databases

    DisGeNETi10437
    OpenTargetsiENSG00000216490
    PharmGKBiPA29644

    Polymorphism and mutation databases

    BioMutaiIFI30
    DMDMi327478582

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 26Sequence analysisAdd BLAST26
    PropeptideiPRO_000002132827 – 57Removed in mature form1 PublicationAdd BLAST31
    ChainiPRO_000002132958 – 232Gamma-interferon-inducible lysosomal thiol reductaseAdd BLAST175
    PropeptideiPRO_0000021330233 – 250Removed in mature form1 PublicationAdd BLAST18

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi72 ↔ 75Redox-active2 Publications
    Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    N-glycosylated. Sugar chains contain mannose-6-phosphate.2 Publications
    Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiP13284
    MaxQBiP13284
    PaxDbiP13284
    PeptideAtlasiP13284
    PRIDEiP13284
    ProteomicsDBi52905

    PTM databases

    iPTMnetiP13284
    PhosphoSitePlusiP13284

    Expressioni

    Inductioni

    Expressed constitutively in antigen-presenting cells and induced by IFN-gamma in other cell types.1 Publication

    Gene expression databases

    BgeeiENSG00000216490
    CleanExiHS_IFI30
    ExpressionAtlasiP13284 baseline and differential
    GenevisibleiP13284 HS

    Organism-specific databases

    HPAiHPA026650

    Interactioni

    Subunit structurei

    Dimer; disulfide-linked.2 Publications

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi115704, 43 interactors
    IntActiP13284, 8 interactors
    STRINGi9606.ENSP00000384886

    Structurei

    3D structure databases

    ProteinModelPortaliP13284
    SMRiP13284
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GILT family.Curated

    Keywords - Domaini

    Redox-active center, Signal

    Phylogenomic databases

    eggNOGiKOG3160 Eukaryota
    ENOG4111IDT LUCA
    GeneTreeiENSGT00390000010450
    HOGENOMiHOG000238423
    HOVERGENiHBG005837
    InParanoidiP13284
    KOiK08059
    OMAiVSGRWEF
    OrthoDBiEOG091G0IMC
    PhylomeDBiP13284
    TreeFamiTF315141

    Family and domain databases

    InterProiView protein in InterPro
    IPR004911 Interferon-induced_GILT
    PANTHERiPTHR13234 PTHR13234, 1 hit
    PfamiView protein in Pfam
    PF03227 GILT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13284-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTLSPLLLFL PPLLLLLDVP TAAVQASPLQ ALDFFGNGPP VNYKTGNLYL
    60 70 80 90 100
    RGPLKKSNAP LVNVTLYYEA LCGGCRAFLI RELFPTWLLV MEILNVTLVP
    110 120 130 140 150
    YGNAQEQNVS GRWEFKCQHG EEECKFNKVE ACVLDELDME LAFLTIVCME
    160 170 180 190 200
    EFEDMERSLP LCLQLYAPGL SPDTIMECAM GDRGMQLMHA NAQRTDALQP
    210 220 230 240 250
    PHEYVPWVTV NGKPLEDQTQ LLTLVCQLYQ GKKPDVCPSS TSSLRSVCFK
    Length:250
    Mass (Da):27,964
    Last modified:April 5, 2011 - v3
    Checksum:i54B4950E3788CD5A
    GO

    Sequence cautioni

    The sequence AAA36105 differs from that shown. Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.Curated
    The sequence AAA36105 differs from that shown. Reason: Frameshift at positions 146 and 174.Curated
    The sequence AAA36105 differs from that shown. Reason: Erroneous termination at position 251. Translated as stop.Curated
    The sequence AAF04618 differs from that shown. Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti76R → Q in AAH31020 (PubMed:15489334).Curated1
    Sequence conflicti98L → S in AAA36105 (PubMed:3136170).Curated1
    Sequence conflicti119H → L in AAA36105 (PubMed:3136170).Curated1
    Sequence conflicti148C → WHG in AAA36105 (PubMed:3136170).Curated1
    Sequence conflicti223T → A in BAC98466 (Ref. 3) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J03909 mRNA Translation: AAA36105.1 Sequence problems.
    AF097362 mRNA Translation: AAF04618.1 Sequence problems.
    AB049659 mRNA Translation: BAC98466.1
    AC007192 Genomic DNA No translation available.
    AC093080 Genomic DNA No translation available.
    AC068499 Genomic DNA No translation available.
    CH471106 Genomic DNA Translation: EAW84662.1
    BC021136 mRNA Translation: AAH21136.1
    BC031020 mRNA Translation: AAH31020.1
    CCDSiCCDS46015.1
    PIRiA43708
    RefSeqiNP_006323.2, NM_006332.4
    UniGeneiHs.14623

    Genome annotation databases

    EnsembliENST00000407280; ENSP00000384886; ENSG00000216490
    GeneIDi10437
    KEGGihsa:10437
    UCSCiuc002nic.3 human

    Similar proteinsi

    Entry informationi

    Entry nameiGILT_HUMAN
    AccessioniPrimary (citable) accession number: P13284
    Secondary accession number(s): Q76MF9
    , Q8NEI4, Q8WU77, Q9UL08
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: April 5, 2011
    Last modified: June 20, 2018
    This is version 140 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

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