UniProtKB - P13272 (UCRI_BOVIN)
Cytochrome b-c1 complex subunit Rieske, mitochondrial
UQCRFS1
Functioni
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q0 site in cytochrome b to cytochrome c1 (By similarity).
Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).
By similarityComponent of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer. One of the fragments, called subunit 9, corresponds to its mitochondrial targeting sequence (MTS) (PubMed:8386158, PubMed:2996928).
The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function (By similarity).
By similarity2 PublicationsMiscellaneous
Caution
Catalytic activityi
- a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H+(out)By similarityEC:7.1.1.8By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 217 | Iron-sulfur (2Fe-2S)Combined sources1 Publication | 1 | |
Metal bindingi | 219 | Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 236 | Iron-sulfur (2Fe-2S)Combined sources1 Publication | 1 | |
Metal bindingi | 239 | Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 241 | Iron-sulfur (2Fe-2S)Combined sources1 Publication | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity Source: GO_Central
- ubiquinol-cytochrome-c reductase activity Source: UniProtKB-EC
GO - Biological processi
- mitochondrial electron transport, ubiquinol to cytochrome c Source: GO_Central
- mitochondrial respiratory chain complex III assembly Source: Ensembl
Keywordsi
Molecular function | Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding |
Protein family/group databases
TCDBi | 3.D.3.2.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:7.1.1.8)Alternative name(s): Complex III subunit 5 Cytochrome b-c1 complex subunit 5 Rieske iron-sulfur protein Short name: RISP Rieske protein UQCRFS1 Ubiquinol-cytochrome c reductase iron-sulfur subunit Cleaved into the following chain: Cytochrome b-c1 complex subunit 91 Publication Short name: Su91 Publication Short name: Subunit 91 Publication Alternative name(s): 8 kDa subunit 91 Publication Complex III subunit IX Cytochrome b-c1 complex subunit 11 UQCRFS1 mitochondrial targeting sequence Short name: UQCRFS1 MTS Ubiquinol-cytochrome c reductase 8 kDa protein1 Publication |
Gene namesi | Name:UQCRFS1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000046786 |
VGNCi | VGNC:53044, UQCRFS1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Single-pass membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: AgBase
- mitochondrial respiratory chain complex III Source: GO_Central
- mitochondrial respiratory chain complex IV Source: Ensembl
- mitochondrion Source: AgBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 79 – 103 | Mitochondrial matrix1 PublicationAdd BLAST | 25 | |
Transmembranei | 104 – 140 | Helical1 PublicationAdd BLAST | 37 | |
Topological domaini | 141 – 274 | Mitochondrial intermembrane1 PublicationAdd BLAST | 134 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030659 | 1 – 78 | Cytochrome b-c1 complex subunit 92 PublicationsAdd BLAST | 78 | |
ChainiPRO_0000030660 | 79 – 274 | Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST | 196 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 222 ↔ 238 | Combined sources2 Publications |
Post-translational modificationi
Keywords - PTMi
Disulfide bondProteomic databases
PeptideAtlasi | P13272 |
PRIDEi | P13272 |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1 (PubMed:8386158). The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI1III2IV1 and megacomplex MCI2III2IV2) (PubMed:27830641). Incorporation of the Rieske protein UQCRFS1 is the penultimate step in complex III assembly.
Interacts with TTC19, which is involved in the clearance of UQCRFS1 fragments (By similarity).
By similarity2 PublicationsComponent of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) (PubMed:8386158). Subunit 9 corresponds to the mitochondrial targeting sequence (MTS) of Rieske protein UQCRFS1. It is retained after processing and incorporated inside complex III, where it remains bound to the complex and localizes between the 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 (PubMed:9651245).
2 PublicationsProtein-protein interaction databases
CORUMi | P13272 |
DIPi | DIP-38974N |
IntActi | P13272, 3 interactors |
STRINGi | 9913.ENSBTAP00000054196 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P13272 |
SMRi | P13272 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13272 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 187 – 272 | RieskePROSITE-ProRule annotationAdd BLAST | 86 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1671, Eukaryota |
GeneTreei | ENSGT00390000001014 |
InParanoidi | P13272 |
OMAi | DFLLNMS |
OrthoDBi | 1174160at2759 |
Family and domain databases
Gene3Di | 1.20.5.270, 1 hit 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR037008, bc1_Rieske_TM_sf IPR011070, Globular_prot_asu/bsu IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR014349, Rieske_Fe-S_prot IPR005805, Rieske_Fe-S_prot_C IPR004192, Rieske_TM IPR015248, Ubiqinol_cyt_c_Rdtase_N IPR006317, Ubiquinol_cyt_c_Rdtase_Fe-S-su |
PANTHERi | PTHR10134, PTHR10134, 1 hit |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF09165, Ubiq-Cytc-red_N, 1 hit PF02921, UCR_TM, 1 hit |
PRINTSi | PR00162, RIESKE |
SUPFAMi | SSF50022, SSF50022, 1 hit SSF56568, SSF56568, 1 hit |
TIGRFAMsi | TIGR01416, Rieske_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLSVAARSGP FAPVLSATSR GVAGALRPLV QAAVPATSES PVLDLKRSVL
60 70 80 90 100
CRESLRGQAA GRPLVASVSL NVPASVRYSH TDIKVPDFSD YRRPEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NVVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PSYEFTSDDM VIVG
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 150 | S → A AA sequence (PubMed:3036596).Curated | 1 | |
Sequence conflicti | 269 | D → G AA sequence (PubMed:3036596).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34336 mRNA Translation: AAA30515.1 Sequence problems. S58789 mRNA Translation: AAB26197.1 BC115994 mRNA Translation: AAI15995.1 BC133620 mRNA Translation: AAI33621.1 BC133632 mRNA Translation: AAI33633.1 |
PIRi | A46063, A34660 |
RefSeqi | NP_777238.1, NM_174813.2 |
Genome annotation databases
Ensembli | ENSBTAT00000066053; ENSBTAP00000054196; ENSBTAG00000046786 |
GeneIDi | 287020 |
KEGGi | bta:287020 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34336 mRNA Translation: AAA30515.1 Sequence problems. S58789 mRNA Translation: AAB26197.1 BC115994 mRNA Translation: AAI15995.1 BC133620 mRNA Translation: AAI33621.1 BC133632 mRNA Translation: AAI33633.1 |
PIRi | A46063, A34660 |
RefSeqi | NP_777238.1, NM_174813.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BCC | X-ray | 3.16 | A | 192-195 | [»] | |
E | 79-274 | [»] | ||||
1BE3 | X-ray | 3.00 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1BGY | X-ray | 3.00 | E/Q | 79-274 | [»] | |
I/U | 1-78 | [»] | ||||
1L0L | X-ray | 2.35 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1L0N | X-ray | 2.60 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1NTK | X-ray | 2.60 | E | 79-274 | [»] | |
I | 1-57 | [»] | ||||
1NTM | X-ray | 2.40 | E | 79-274 | [»] | |
I | 1-57 | [»] | ||||
1NTZ | X-ray | 2.60 | E | 79-274 | [»] | |
I | 1-57 | [»] | ||||
1NU1 | X-ray | 3.20 | E | 79-274 | [»] | |
I | 1-57 | [»] | ||||
1PP9 | X-ray | 2.10 | E/R | 79-274 | [»] | |
I/V | 1-78 | [»] | ||||
1PPJ | X-ray | 2.10 | E/R | 79-274 | [»] | |
I/V | 1-78 | [»] | ||||
1QCR | X-ray | 2.70 | E | 79-274 | [»] | |
I | 21-48 | [»] | ||||
1RIE | X-ray | 1.50 | A | 146-274 | [»] | |
1SQB | X-ray | 2.69 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1SQP | X-ray | 2.70 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1SQQ | X-ray | 3.00 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1SQV | X-ray | 2.85 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
1SQX | X-ray | 2.60 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
2A06 | X-ray | 2.10 | E/R | 79-274 | [»] | |
I/V | 1-78 | [»] | ||||
2FYU | X-ray | 2.26 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
2YBB | electron microscopy | 19.00 | E/e | 79-274 | [»] | |
I/i | 14-78 | [»] | ||||
4D6T | X-ray | 3.57 | E/I/R/V | 1-274 | [»] | |
4D6U | X-ray | 4.09 | E/I/R/V | 1-274 | [»] | |
5GPN | electron microscopy | 5.40 | I/U | 1-78 | [»] | |
5KLV | X-ray | 2.65 | E | 79-274 | [»] | |
I | 1-78 | [»] | ||||
5LUF | electron microscopy | 9.10 | e/q | 79-274 | [»] | |
i/u | 1-78 | [»] | ||||
5NMI | X-ray | 3.50 | E/I/R/V | 1-274 | [»] | |
6FO0 | electron microscopy | 4.10 | E/R | 1-274 | [»] | |
6FO6 | electron microscopy | 4.10 | E/R | 1-274 | [»] | |
AlphaFoldDBi | P13272 | |||||
SMRi | P13272 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | P13272 |
DIPi | DIP-38974N |
IntActi | P13272, 3 interactors |
STRINGi | 9913.ENSBTAP00000054196 |
Protein family/group databases
TCDBi | 3.D.3.2.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Proteomic databases
PeptideAtlasi | P13272 |
PRIDEi | P13272 |
Genome annotation databases
Ensembli | ENSBTAT00000066053; ENSBTAP00000054196; ENSBTAG00000046786 |
GeneIDi | 287020 |
KEGGi | bta:287020 |
Organism-specific databases
CTDi | 7386 |
VEuPathDBi | HostDB:ENSBTAG00000046786 |
VGNCi | VGNC:53044, UQCRFS1 |
Phylogenomic databases
eggNOGi | KOG1671, Eukaryota |
GeneTreei | ENSGT00390000001014 |
InParanoidi | P13272 |
OMAi | DFLLNMS |
OrthoDBi | 1174160at2759 |
Miscellaneous databases
EvolutionaryTracei | P13272 |
Gene expression databases
ExpressionAtlasi | P13272, baseline and differential |
Family and domain databases
Gene3Di | 1.20.5.270, 1 hit 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR037008, bc1_Rieske_TM_sf IPR011070, Globular_prot_asu/bsu IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR014349, Rieske_Fe-S_prot IPR005805, Rieske_Fe-S_prot_C IPR004192, Rieske_TM IPR015248, Ubiqinol_cyt_c_Rdtase_N IPR006317, Ubiquinol_cyt_c_Rdtase_Fe-S-su |
PANTHERi | PTHR10134, PTHR10134, 1 hit |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF09165, Ubiq-Cytc-red_N, 1 hit PF02921, UCR_TM, 1 hit |
PRINTSi | PR00162, RIESKE |
SUPFAMi | SSF50022, SSF50022, 1 hit SSF56568, SSF56568, 1 hit |
TIGRFAMsi | TIGR01416, Rieske_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UCRI_BOVIN | |
Accessioni | P13272Primary (citable) accession number: P13272 Secondary accession number(s): A3KN53, P07588, Q1LZH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | February 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 185 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families