Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

UQCRFS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).By similarity
Cytochrome b-c1 complex subunit 9: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (PubMed:2996928, PubMed:8386158). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer (PubMed:2996928, PubMed:8386158). One of the fragments, called subunit 9, corresponds to the transit peptide (PubMed:2996928, PubMed:8386158). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function (By similarity). It is unsure whether the UQCRFS1 fragments, including subunit 9, are structural subunits (By similarity).By similarity2 Publications

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Caution

Several peptides are generated during UQCRFS1 insertion. According to some authors, the identification of the transit peptide as the subunit 9, does not necessary imply that it must be considered as a structural subunit of the complex III dimer as additional fragments from UQCRFS1 are also present.By similarity

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit. Fe-S cluster delivery to the Rieske protein is mediated by components of the iron sulfur (Fe-S) cluster assembly machinery that reside in the mitochondrial matrix (including HSC20 and LYRM7) (By similarity).PROSITE-ProRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi217Iron-sulfur (2Fe-2S)1
Metal bindingi219Iron-sulfur (2Fe-2S); via pros nitrogen1
Metal bindingi236Iron-sulfur (2Fe-2S)1
Metal bindingi239Iron-sulfur (2Fe-2S); via pros nitrogen1

GO - Molecular functioni

GO - Biological processi

  • mitochondrial electron transport, ubiquinol to cytochrome c Source: GO_Central

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Protein family/group databases

TCDBi3.D.3.2.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Rieske protein UQCRFS1
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Cytochrome b-c1 complex subunit 91 Publication
Short name:
Su91 Publication
Short name:
Subunit 91 Publication
Alternative name(s):
8 kDa subunit 91 Publication
Complex III subunit IX
Cytochrome b-c1 complex subunit 11
Ubiquinol-cytochrome c reductase 8 kDa protein1 Publication
Gene namesi
Name:UQCRFS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei103 – 140HelicalAdd BLAST38

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 78Mitochondrion2 PublicationsAdd BLAST78
ChainiPRO_00000306591 – 78Cytochrome b-c1 complex subunit 91 PublicationAdd BLAST78
ChainiPRO_000003066079 – 274Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi222 ↔ 238PROSITE-ProRule annotation1 Publication

Post-translational modificationi

Proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer (By similarity). Several fragments are generated during UQCRFS1 insertion, most probably due to the endogenous matrix-processing peptidase (MPP) activity (By similarity). The action of the protease is also necessary for the clearance of the UQCRFS1 fragments (By similarity).By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PeptideAtlasiP13272
PRIDEiP13272

Interactioni

Subunit structurei

Binds to the mitochondrial respiratory complex III dimer. The monomeric module of the mitochondrial respiratory complex III contains 11 subunits: 3 respiratory subunits involved in its catalytic activity (cytochrome b, cytochrome c1 and Rieske protein UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1) (PubMed:8386158). Incorporation of the Rieske protein UQCRFS1 is the penultimate step in complex III assembly (By similarity). Interacts with TTC19, which is involved in the clearance of UQCRFS1 fragments (By similarity).By similarity1 Publication

Protein-protein interaction databases

CORUMiP13272
DIPiDIP-38974N
IntActiP13272, 3 interactors

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13272
SMRiP13272
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13272

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 272RieskePROSITE-ProRule annotationAdd BLAST86

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG001040
InParanoidiP13272
KOiK00411

Family and domain databases

Gene3Di1.20.5.270, 1 hit
2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR037008 bc1_Rieske_TM_sf
IPR011070 Globular_prot_asu/bsu
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR014349 Rieske_Fe-S_prot
IPR005805 Rieske_Fe-S_prot_C
IPR004192 Rieske_TM
IPR015248 Ubiqinol_cyt_c_Rdtase_N
IPR006317 Ubiquinol_cyt_c_Rdtase_Fe-S-su
PANTHERiPTHR10134 PTHR10134, 1 hit
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF09165 Ubiq-Cytc-red_N, 1 hit
PF02921 UCR_TM, 1 hit
PRINTSiPR00162 RIESKE
SUPFAMiSSF50022 SSF50022, 1 hit
SSF56568 SSF56568, 1 hit
TIGRFAMsiTIGR01416 Rieske_proteo, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13272-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSVAARSGP FAPVLSATSR GVAGALRPLV QAAVPATSES PVLDLKRSVL
60 70 80 90 100
CRESLRGQAA GRPLVASVSL NVPASVRYSH TDIKVPDFSD YRRPEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NVVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PSYEFTSDDM VIVG
Length:274
Mass (Da):29,547
Last modified:February 1, 1996 - v3
Checksum:i7C5FC17D2A0DD1C9
GO

Sequence cautioni

The sequence AAA30515 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150S → A AA sequence (PubMed:3036596).Curated1
Sequence conflicti269D → G AA sequence (PubMed:3036596).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34336 mRNA Translation: AAA30515.1 Sequence problems.
S58789 mRNA Translation: AAB26197.1
BC115994 mRNA Translation: AAI15995.1
BC133620 mRNA Translation: AAI33621.1
BC133632 mRNA Translation: AAI33633.1
PIRiA46063 A34660
RefSeqiNP_777238.1, NM_174813.2
UniGeneiBt.265

Genome annotation databases

GeneIDi287020
KEGGibta:287020

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34336 mRNA Translation: AAA30515.1 Sequence problems.
S58789 mRNA Translation: AAB26197.1
BC115994 mRNA Translation: AAI15995.1
BC133620 mRNA Translation: AAI33621.1
BC133632 mRNA Translation: AAI33633.1
PIRiA46063 A34660
RefSeqiNP_777238.1, NM_174813.2
UniGeneiBt.265

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BCCX-ray3.16A192-195[»]
E79-274[»]
1BE3X-ray3.00E79-274[»]
I1-78[»]
1BGYX-ray3.00E/Q79-274[»]
I/U1-78[»]
1L0LX-ray2.35E79-274[»]
I1-78[»]
1L0NX-ray2.60E79-274[»]
I1-78[»]
1NTKX-ray2.60E79-274[»]
I1-57[»]
1NTMX-ray2.40E79-274[»]
I1-57[»]
1NTZX-ray2.60E79-274[»]
I1-57[»]
1NU1X-ray3.20E79-274[»]
I1-57[»]
1PP9X-ray2.10E/R79-274[»]
I/V1-78[»]
1PPJX-ray2.10E/R79-274[»]
I/V1-78[»]
1QCRX-ray2.70E79-274[»]
I21-48[»]
1RIEX-ray1.50A146-274[»]
1SQBX-ray2.69E79-274[»]
I1-78[»]
1SQPX-ray2.70E79-274[»]
I1-78[»]
1SQQX-ray3.00E79-274[»]
I1-78[»]
1SQVX-ray2.85E79-274[»]
I1-78[»]
1SQXX-ray2.60E79-274[»]
I1-78[»]
2A06X-ray2.10E/R79-274[»]
I/V1-78[»]
2FYUX-ray2.26E79-274[»]
I1-78[»]
2YBBelectron microscopy19.00E/e79-274[»]
I/i14-78[»]
4D6TX-ray3.57E/I/R/V1-274[»]
4D6UX-ray4.09E/I/R/V1-274[»]
5GPNelectron microscopy5.40I/U1-78[»]
5KLVX-ray2.65E79-274[»]
I1-78[»]
5LUFelectron microscopy9.10e/q79-274[»]
i/u1-78[»]
5NMIX-ray3.50E/I/R/V1-274[»]
6FO0electron microscopy4.10E/R1-274[»]
6FO6electron microscopy4.10E/R1-274[»]
ProteinModelPortaliP13272
SMRiP13272
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP13272
DIPiDIP-38974N
IntActiP13272, 3 interactors

Protein family/group databases

TCDBi3.D.3.2.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Proteomic databases

PeptideAtlasiP13272
PRIDEiP13272

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287020
KEGGibta:287020

Organism-specific databases

CTDi7386

Phylogenomic databases

HOVERGENiHBG001040
InParanoidiP13272
KOiK00411

Miscellaneous databases

EvolutionaryTraceiP13272

Family and domain databases

Gene3Di1.20.5.270, 1 hit
2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR037008 bc1_Rieske_TM_sf
IPR011070 Globular_prot_asu/bsu
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR014349 Rieske_Fe-S_prot
IPR005805 Rieske_Fe-S_prot_C
IPR004192 Rieske_TM
IPR015248 Ubiqinol_cyt_c_Rdtase_N
IPR006317 Ubiquinol_cyt_c_Rdtase_Fe-S-su
PANTHERiPTHR10134 PTHR10134, 1 hit
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF09165 Ubiq-Cytc-red_N, 1 hit
PF02921 UCR_TM, 1 hit
PRINTSiPR00162 RIESKE
SUPFAMiSSF50022 SSF50022, 1 hit
SSF56568 SSF56568, 1 hit
TIGRFAMsiTIGR01416 Rieske_proteo, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUCRI_BOVIN
AccessioniPrimary (citable) accession number: P13272
Secondary accession number(s): A3KN53, P07588, Q1LZH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again