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UniProtKB - P13267 (DPO3_BACSU)
Protein
DNA polymerase III PolC-type
Gene
polC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
Miscellaneous
Mutant azp12 has a form of DNA polymerase III resistant to hydroxyphenylazopyrimidines.
Catalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- 3'-5' exonuclease activity Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA-directed DNA polymerase activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | Antibiotic resistance, DNA replication |
Enzyme and pathway databases
BioCyci | BSUB:BSU16580-MONOMER |
SABIO-RKi | P13267 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:polC Synonyms:dnaF, mutI Ordered Locus Names:BSU16580 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 427 | E → A: Destroys both polymerase and exonuclease activities. 1 Publication | 1 | |
Mutagenesisi | 427 | E → Q: Destroys exonuclease activity; decreases polymerase activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3897 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204573 | 1 – 1437 | DNA polymerase III PolC-typeAdd BLAST | 1437 |
Proteomic databases
PaxDbi | P13267 |
PRIDEi | P13267 |
Interactioni
Protein-protein interaction databases
IntActi | P13267, 3 interactors |
STRINGi | 224308.BSU16580 |
Chemistry databases
BindingDBi | P13267 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 421 – 578 | ExonucleaseAdd BLAST | 158 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 613 – 1437 | dNTP polymerization siteSequence analysisAdd BLAST | 825 | |
Regioni | 1393 – 1437 | Contains the active site of the enzymeAdd BLAST | 45 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2176, Bacteria |
InParanoidi | P13267 |
OMAi | YYAAYFT |
PhylomeDBi | P13267 |
Family and domain databases
Gene3Di | 1.10.150.700, 1 hit 3.30.420.10, 1 hit |
HAMAPi | MF_00356, DNApol_PolC, 1 hit |
InterProi | View protein in InterPro IPR011708, DNA_pol3_alpha_NTPase_dom IPR040982, DNA_pol3_finger IPR024754, DNA_PolC-like_N_II IPR028112, DNA_PolC-type_N_I IPR029460, DNAPol_HHH IPR006054, DnaQ IPR013520, Exonuclease_RNaseT/DNA_pol3 IPR012340, NA-bd_OB-fold IPR004013, PHP_dom IPR003141, Pol/His_phosphatase_N IPR006308, PolC_gram_pos IPR044923, PolC_middle_finger_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF14480, DNA_pol3_a_NI, 1 hit PF11490, DNA_pol3_a_NII, 1 hit PF07733, DNA_pol3_alpha, 2 hits PF17657, DNA_pol3_finger, 1 hit PF14579, HHH_6, 1 hit PF02811, PHP, 1 hit PF00929, RNase_T, 1 hit |
SMARTi | View protein in SMART SM00479, EXOIII, 1 hit SM00481, POLIIIAc, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF53098, SSF53098, 1 hit |
TIGRFAMsi | TIGR00573, dnaq, 1 hit TIGR01405, polC_Gram_pos, 1 hit |
i Sequence
Sequence statusi: Complete.
P13267-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQLSVNRRQ FQILLQQINM TDDTFMTYFE HGEIKKLTIH KASKSWHFHF
60 70 80 90 100
QFKSLLPFQI YDTLTTRLTQ SFAHIAKVTS SIEVQDAEVS ESIVQDYWSR
110 120 130 140 150
CIEELQGISP PIISLLNQQK PKLKGNKLIV KTKTDTEAAA LKNKYSSMIQ
160 170 180 190 200
AEYRQFGFPD LQLDAEIFVS EQEVQKFREQ KLAEDQERAM QALIEMEKKD
210 220 230 240 250
KESDEDQAPS GPLVIGYQIK DNEEIRTLDS IMDEERRITV QGYVFDVETR
260 270 280 290 300
ELKSGRTLCI FKITDYTNSI LIKMFAREKE DAALMKSLKK GMWVKARGSI
310 320 330 340 350
QNDTFVRDLV MIANDVNEIK AKTREDSAPE GEKRVELHLH SPMSQMDAVT
360 370 380 390 400
GIGKLVEQAK KWGHEAIALT DHAVVQSFPD AYSAAKKHGI KMIYGMEANL
410 420 430 440 450
VDDGVPIAYN AAHRLLEEET YVVFDVETTG LSAVYDTIIE LAAVKVKGGE
460 470 480 490 500
IIDKFEAFAN PHRPLSATII ELTGITDDML QDAPDVVDVI RDFREWIGDD
510 520 530 540 550
ILVAHNASFD MGFLNVAYKK LLEVEKAKNP VIDTLELGRF LYPEFKNHRL
560 570 580 590 600
NTLCKKFDIE LTQHHRAIYD TEATAYLLLK MLKDAAEKGI QYHDELNENM
610 620 630 640 650
GQSNAYQRSR PYHATLLAVN STGLKNLFKL VSLSHIHYFY RVPRIPRSQL
660 670 680 690 700
EKYREGLLIG SACDRGEVFE GMMQKSPEEV EDIASFYDYL EVQPPEVYRH
710 720 730 740 750
LLELELVRDE KALKEIIANI TKLGEKLNKP VVATGNVHYL NDEDKIYRKI
760 770 780 790 800
LISSQGGANP LNRHELPKVH FRTTDEMLEA FSFLGEEKAK EIVVTNTQKV
810 820 830 840 850
ASLVDDIKPI KDDLYTPKIE GADEEIREMS YQRARSIYGE ELPEIVEARI
860 870 880 890 900
EKELKSIIGH GFAVIYLISH KLVKRSLDDG YLVGSRGSVG SSLVATLTEI
910 920 930 940 950
TEVNPLPPHY VCPECQHSEF FNDGSVGSGF DLPDKTCPHC GTPLKKDGHD
960 970 980 990 1000
IPFETFLGFK GDKVPDIDLN FSGEYQPQAH NYTKVLFGED NVYRAGTIGT
1010 1020 1030 1040 1050
VAEKTAYGYV KGYAGDNNLH MRGAEIDRLV QGCTGVKRTT GQHPGGIIVV
1060 1070 1080 1090 1100
PDYMDIYDFS PIQFPADATG SEWKTTHFDF HSIHDNLLKL DILGHDDPTV
1110 1120 1130 1140 1150
IRMLQDLSGI DPKTIPTDDP EVMKIFQGTE SLGVTEEQIG CKTGTLGIPE
1160 1170 1180 1190 1200
FGTRFVRQML EDTKPTTFSE LVQISGLSHG TDVWLGNAQE LIHNNICELS
1210 1220 1230 1240 1250
EVIGCRDDIM VYLIYQGLEP SLAFKIMEFV RKGKGLTPEW EEEMKNNNVP
1260 1270 1280 1290 1300
DWYIDSCKKI KYMFPKAHAA AYVLMAVRIA YFKVHHALLY YAAYFTVRAD
1310 1320 1330 1340 1350
DFDIDTMIKG STAIRAVMED INAKGLDASP KEKNLLTVLE LALEMCERGY
1360 1370 1380 1390 1400
SFQKVDLYRS SATEFIIDGN SLIPPFNSIP GLGTNAALNI VKAREEGEFL
1410 1420 1430
SKEDLQKRGK VSKTILEYLD RHGCLESLPD QNQLSLF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 – 188 | EDQER → KTKSW in AAA22666 (PubMed:2499883).Curated | 5 | |
Sequence conflicti | 495 – 496 | EW → VR in AAA22666 (PubMed:2499883).Curated | 2 | |
Sequence conflicti | 829 | M → S in AAA22666 (PubMed:2499883).Curated | 1 | |
Sequence conflicti | 1015 | G → E in AAA22666 (PubMed:2499883).Curated | 1 | |
Sequence conflicti | 1190 | E → D in AAA22666 (PubMed:2499883).Curated | 1 | |
Sequence conflicti | 1405 – 1406 | LQ → FE in AAA22666 (PubMed:2499883).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 1175 | S → A in mutant AZP12. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52116 Genomic DNA Translation: CAA36362.1 M22996 Genomic DNA Translation: AAA22666.1 AL009126 Genomic DNA Translation: CAB13531.1 S55653 Genomic DNA Translation: AAP13898.1 M33543 Genomic DNA Translation: AAA22667.1 Z18631 Genomic DNA No translation available. |
PIRi | F69680 |
RefSeqi | NP_389540.1, NC_000964.3 WP_003245843.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13531; CAB13531; BSU_16580 |
GeneIDi | 939620 |
KEGGi | bsu:BSU16580 |
PATRICi | fig|224308.179.peg.1799 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52116 Genomic DNA Translation: CAA36362.1 M22996 Genomic DNA Translation: AAA22666.1 AL009126 Genomic DNA Translation: CAB13531.1 S55653 Genomic DNA Translation: AAP13898.1 M33543 Genomic DNA Translation: AAA22667.1 Z18631 Genomic DNA No translation available. |
PIRi | F69680 |
RefSeqi | NP_389540.1, NC_000964.3 WP_003245843.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | P13267 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P13267, 3 interactors |
STRINGi | 224308.BSU16580 |
Chemistry databases
BindingDBi | P13267 |
ChEMBLi | CHEMBL3897 |
Proteomic databases
PaxDbi | P13267 |
PRIDEi | P13267 |
Genome annotation databases
EnsemblBacteriai | CAB13531; CAB13531; BSU_16580 |
GeneIDi | 939620 |
KEGGi | bsu:BSU16580 |
PATRICi | fig|224308.179.peg.1799 |
Phylogenomic databases
eggNOGi | COG2176, Bacteria |
InParanoidi | P13267 |
OMAi | YYAAYFT |
PhylomeDBi | P13267 |
Enzyme and pathway databases
BioCyci | BSUB:BSU16580-MONOMER |
SABIO-RKi | P13267 |
Miscellaneous databases
PROi | PR:P13267 |
Family and domain databases
Gene3Di | 1.10.150.700, 1 hit 3.30.420.10, 1 hit |
HAMAPi | MF_00356, DNApol_PolC, 1 hit |
InterProi | View protein in InterPro IPR011708, DNA_pol3_alpha_NTPase_dom IPR040982, DNA_pol3_finger IPR024754, DNA_PolC-like_N_II IPR028112, DNA_PolC-type_N_I IPR029460, DNAPol_HHH IPR006054, DnaQ IPR013520, Exonuclease_RNaseT/DNA_pol3 IPR012340, NA-bd_OB-fold IPR004013, PHP_dom IPR003141, Pol/His_phosphatase_N IPR006308, PolC_gram_pos IPR044923, PolC_middle_finger_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF14480, DNA_pol3_a_NI, 1 hit PF11490, DNA_pol3_a_NII, 1 hit PF07733, DNA_pol3_alpha, 2 hits PF17657, DNA_pol3_finger, 1 hit PF14579, HHH_6, 1 hit PF02811, PHP, 1 hit PF00929, RNase_T, 1 hit |
SMARTi | View protein in SMART SM00479, EXOIII, 1 hit SM00481, POLIIIAc, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF53098, SSF53098, 1 hit |
TIGRFAMsi | TIGR00573, dnaq, 1 hit TIGR01405, polC_Gram_pos, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO3_BACSU | |
Accessioni | P13267Primary (citable) accession number: P13267 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | August 1, 1991 | |
Last modified: | September 29, 2021 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families