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Protein

Probable fructose-bisphosphate aldolase

Gene

fbaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Probable fructose-bisphosphate aldolase (fbaA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Glyceraldehyde 3-phosphateBy similarity1
Active sitei85Proton donorBy similarity1
Metal bindingi86Zinc 1; catalyticBy similarity1
Metal bindingi107Zinc 2By similarity1
Metal bindingi137Zinc 2By similarity1
Metal bindingi181Zinc 1; catalyticBy similarity1
Binding sitei182Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi209Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis, Sporulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU37120-MONOMER
SABIO-RKiP13243
UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Probable fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fbaA
Synonyms:fba, fba1, tsr
Ordered Locus Names:BSU37120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787051 – 285Probable fructose-bisphosphate aldolaseAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212Phosphothreonine1 Publication1
Modified residuei234Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated during sporulation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13243
PRIDEiP13243

PTM databases

iPTMnetiP13243

Interactioni

Protein-protein interaction databases

IntActiP13243, 1 interactor
MINTiP13243
STRINGi224308.Bsubs1_010100020066

Structurei

3D structure databases

ProteinModelPortaliP13243
SMRiP13243
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 212Dihydroxyacetone phosphate bindingBy similarity3
Regioni231 – 234Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D2N Bacteria
COG0191 LUCA
HOGENOMiHOG000227793
InParanoidiP13243
KOiK01624
OMAiELCKDCI
PhylomeDBiP13243

Family and domain databases

CDDicd00947 TBP_aldolase_IIB, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR011289 Fruc_bis_ald_class-2
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01859 fruc_bis_ald_, 1 hit
PROSITEiView protein in PROSITE
PS00602 ALDOLASE_CLASS_II_1, 1 hit
PS00806 ALDOLASE_CLASS_II_2, 1 hit

Sequencei

Sequence statusi: Complete.

P13243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLVSMTEML NTAKEKGYAV GQFNLNNLEF TQAILQAAEE EKSPVILGVS
60 70 80 90 100
EGAGRYMGGF KTVVAMVKAL MEEYKVTVPV AIHLDHGSSF ESCAKAIHAG
110 120 130 140 150
FTSVMIDASH HPFEENVATT AKVVELAHFH GVSVEAELGT VGGQEDDVIA
160 170 180 190 200
EGVIYADPKE CQELVERTGI DCLAPALGSV HGPYKGEPNL GFKEMEEIGK
210 220 230 240 250
STGLPLVLHG GTGIPTADIK KSISLGTAKI NVNTENQISS AKAVRETLAA
260 270 280
KPDEYDPRKY LGPAREAIKE TVIGKMREFG SSNQA
Length:285
Mass (Da):30,401
Last modified:June 1, 1994 - v2
Checksum:i482D3CE048583BCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22039 Unassigned DNA Translation: AAA16803.1
S42590 Genomic DNA Translation: AAB22716.1
Z49782 Genomic DNA Translation: CAA89873.1
AL009126 Genomic DNA Translation: CAB15729.1
PIRiS55426 D32354
RefSeqiNP_391593.1, NC_000964.3
WP_003243339.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB15729; CAB15729; BSU37120
GeneIDi937040
KEGGibsu:BSU37120
PATRICifig|224308.179.peg.4021

Similar proteinsi

Entry informationi

Entry nameiALF_BACSU
AccessioniPrimary (citable) accession number: P13243
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 1994
Last modified: February 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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