Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5-aminolevulinate synthase, nonspecific, mitochondrial

Gene

Alas1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, nonspecific, mitochondrial (Alas1), 5-aminolevulinate synthase (Alas2), 5-aminolevulinate synthase (Alas1), 5-aminolevulinate synthase (Alas2), 5-aminolevulinate synthase (Alas1), 5-aminolevulinate synthase, erythroid-specific, mitochondrial (Alas2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei219SubstrateBy similarity1
Binding sitei336SubstrateBy similarity1
Binding sitei355SubstrateBy similarity1
Binding sitei388Pyridoxal phosphateBy similarity1
Binding sitei416Pyridoxal phosphateBy similarity1
Binding sitei444Pyridoxal phosphateBy similarity1
Active sitei447By similarity1
Binding sitei476Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei477Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei564SubstrateBy similarity1

GO - Molecular functioni

  • 5-aminolevulinate synthase activity Source: RGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • cellular response to insulin stimulus Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  • response to cAMP Source: RGD
  • response to cobalt ion Source: RGD
  • response to drug Source: RGD
  • response to ethanol Source: RGD
  • response to gonadotropin Source: RGD
  • response to herbicide Source: RGD
  • response to hypoxia Source: RGD
  • response to nickel cation Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to platinum ion Source: RGD

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processHeme biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, nonspecific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-H
Alternative name(s):
5-aminolevulinic acid synthase 1
Delta-ALA synthase 1
Delta-aminolevulinate synthase 1
Gene namesi
Name:Alas1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68392 Alas1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56MitochondrionAdd BLAST56
ChainiPRO_000000123257 – 6425-aminolevulinate synthase, nonspecific, mitochondrialAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei447N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP13195
PRIDEiP13195

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065087

Structurei

3D structure databases

ProteinModelPortaliP13195
SMRiP13195
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1360 Eukaryota
COG0156 LUCA
HOVERGENiHBG005954
InParanoidiP13195
KOiK00643
PhylomeDBiP13195

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR010961 4pyrrol_synth_NH2levulA_synth
IPR015118 5aminolev_synth_preseq
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PF09029 Preseq_ALAS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01821 5aminolev_synth, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METVVRRCPF LSRVPQAFLQ KAGKSLLFYA QNCPKMMEVG AKPAPRTVST
60 70 80 90 100
SAAQCQQVKE TPPANEKEKT AKAAVQQAPD ESQMAQTPDG TQLPPGHPSP
110 120 130 140 150
STSQSSGSKC PFLAAQLSQT GSSVFRKASL ELQEDVQEMH AVRKEVAQSP
160 170 180 190 200
VLPSLVNAKR DGEGPSPLLK NFQDIMRKQR PERVSHLLQD NLPKSVSTFQ
210 220 230 240 250
YDHFFEKKID EKKNDHTYRV FKTVNRRAQI FPMADDYTDS LITKKQVSVW
260 270 280 290 300
CSNDYLGMSR HPRVCGAVIE TVKQHGAGAG GTRNISGTSK FHVELEQELA
310 320 330 340 350
DLHGKDAALL FSSCFVANDS TLFTLAKMMP GCEIYSDSGN HASMIQGIRN
360 370 380 390 400
SRVPKYIFRH NDVNHLRELL QRSDPSVPKI VAFETVHSMD GAVCPLEELC
410 420 430 440 450
DVAHEFGAIT FVDEVHAVGL YGASGGGIGD RDGVMPKMDI ISGTLGKAFG
460 470 480 490 500
CVGGYIASTS LLIDTVRSYA AGFIFTTSLP PMLLAGALES VRILKSNEGR
510 520 530 540 550
ALRRQHQRNV KLMRQMLMDA GLPVIHCPSH IIPVRVADAA KNTEICDELM
560 570 580 590 600
TRHNIYVQAI NYPTVPRGEE LLRIAPTPHH TPQMMNFFLE KLLLTWKRVG
610 620 630 640
LELKPHSSAE CNFCRRPLHF EVMSEREKAY FSGMSKMVSA QA
Length:642
Mass (Da):71,021
Last modified:September 27, 2004 - v2
Checksum:iB28FC05D5F834886
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118 – 136SQTGS…LQEDV → ARRAAASSARPVWSFRRTW in AAA40790 (PubMed:3356687).CuratedAdd BLAST19
Sequence conflicti144K → T in AAA40790 (PubMed:3356687).Curated1
Sequence conflicti195S → V in AAA40790 (PubMed:3356687).Curated1
Sequence conflicti244 – 245KK → NN in AAA40790 (PubMed:3356687).Curated2
Sequence conflicti251C → S in AAA40790 (PubMed:3356687).Curated1
Sequence conflicti377V → L in AAA40724 (PubMed:3182776).Curated1
Sequence conflicti587F → Y in AAA40790 (PubMed:3356687).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03190 mRNA Translation: AAA40790.1
J04044 mRNA Translation: AAA40724.1
BC061793 mRNA Translation: AAH61793.1
PIRiA28191 SYRTAL
RefSeqiNP_077810.2, NM_024484.2
UniGeneiRn.97126

Genome annotation databases

GeneIDi65155
KEGGirno:65155

Similar proteinsi

Entry informationi

Entry nameiHEM1_RAT
AccessioniPrimary (citable) accession number: P13195
Secondary accession number(s): Q6P782
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: September 27, 2004
Last modified: May 23, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health