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UniProtKB - P13188 (SYQ_YEAST)
Protein
Glutamine--tRNA ligase
Gene
GLN4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 37500 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.1.1.18By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 291 | L-glutamineBy similarity | 1 | |
Binding sitei | 440 | L-glutamineBy similarity | 1 | |
Binding sitei | 459 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 259 – 261 | ATPBy similarity | 3 | |
Nucleotide bindingi | 265 – 271 | ATPBy similarity | 7 | |
Nucleotide bindingi | 488 – 489 | ATPBy similarity | 2 | |
Nucleotide bindingi | 496 – 498 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamine-tRNA ligase activity Source: SGD
- mRNA binding Source: SGD
GO - Biological processi
- glutaminyl-tRNA aminoacylation Source: SGD
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.18, 984 6.3.5.7, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamine--tRNA ligase (EC:6.1.1.18By similarity)Alternative name(s): Glutaminyl-tRNA synthetase Short name: GlnRS |
Gene namesi | Name:GLN4 Ordered Locus Names:YOR168W ORF Names:O3601 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005694, GLN4 |
VEuPathDBi | FungiDB:YOR168W |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195866 | 1 – 809 | Glutamine--tRNA ligaseAdd BLAST | 809 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 378 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P13188 |
PaxDbi | P13188 |
PRIDEi | P13188 |
PTM databases
iPTMneti | P13188 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34564, 264 interactors |
IntActi | P13188, 1 interactor |
MINTi | P13188 |
STRINGi | 4932.YOR168W |
Miscellaneous databases
RNActi | P13188, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P13188 |
SMRi | P13188 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 185 – 216 | DisorderedSequence analysisAdd BLAST | 32 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 258 – 268 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 495 – 499 | 'KMSKS' region | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 185 – 203 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG1148, Eukaryota |
GeneTreei | ENSGT00940000168831 |
HOGENOMi | CLU_001882_2_3_1 |
InParanoidi | P13188 |
OMAi | INNFCAQ |
Family and domain databases
Gene3Di | 1.10.10.2420, 1 hit 1.10.8.1290, 1 hit 2.40.240.10, 2 hits 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR004514, Gln-tRNA-synth IPR007638, Gln-tRNA-synth_Ib_RNA-bd_2 IPR007639, Gln-tRNA-synth_Ib_RNA-bd_N IPR042558, Gln-tRNA-synth_Ib_RNA-bd_N_1 IPR042559, Gln-tRNA-synth_Ib_RNA-bd_N_2 IPR000924, Glu/Gln-tRNA-synth IPR020058, Glu/Gln-tRNA-synth_Ib_cat-dom IPR020059, Glu/Gln-tRNA-synth_Ib_codon-bd IPR020056, Rbsml_L25/Gln-tRNA_synth_N IPR011035, Ribosomal_L25/Gln-tRNA_synth IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749, tRNA-synt_1c, 1 hit PF03950, tRNA-synt_1c_C, 1 hit PF04558, tRNA_synt_1c_R1, 1 hit PF04557, tRNA_synt_1c_R2, 1 hit |
PRINTSi | PR00987, TRNASYNTHGLU |
SUPFAMi | SSF50715, SSF50715, 1 hit |
TIGRFAMsi | TIGR00440, glnS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P13188-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS
60 70 80 90 100
TRALVHNLAS FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE
110 120 130 140 150
ASTKMGMNEN SGVGIEITED QVRNYVMQYI QENKERILTE RYKLVPGIFA
160 170 180 190 200
DVKNLKELKW ADPRSFKPII DQEVLKLLGP KDERDLIKKK TKNNEKKKTN
210 220 230 240 250
SAKKSSDNSA SSGPKRTMFN EGFLGDLHKV GENPQAYPEL MKEHLEVTGG
260 270 280 290 300
KVRTRFPPEP NGYLHIGHSK AIMVNFGYAK YHNGTCYLRF DDTNPEKEAP
310 320 330 340 350
EYFESIKRMV SWLGFKPWKI TYSSDYFDEL YRLAEVLIKN GKAYVCHCTA
360 370 380 390 400
EEIKRGRGIK EDGTPGGERY ACKHRDQSIE QNLQEFRDMR DGKYKPGEAI
410 420 430 440 450
LRMKQDLNSP SPQMWDLIAY RVLNAPHPRT GTKWRIYPTY DFTHCLVDSM
460 470 480 490 500
ENITHSLCTT EFYLSRESYE WLCDQVHVFR PAQREYGRLN ITGTVLSKRK
510 520 530 540 550
IAQLVDEKFV RGWDDPRLFT LEAIRRRGVP PGAILSFINT LGVTTSTTNI
560 570 580 590 600
QVVRFESAVR KYLEDTTPRL MFVLDPVEVV VDNLSDDYEE LATIPYRPGT
610 620 630 640 650
PEFGERTVPF TNKFYIERSD FSENVDDKEF FRLTPNQPVG LIKVSHTVSF
660 670 680 690 700
KSLEKDEAGK IIRIHVNYDN KVEEGSKPKK PKTYIQWVPI SSKYNSPLRV
710 720 730 740 750
TETRVYNQLF KSENPSSHPE GFLKDINPES EVVYKESVME HNFGDVVKNS
760 770 780 790 800
PWVVDSVKNS EFYVEEDKDS KEVCRFQAMR VGYFTLDKES TTSKVILNRI
VSLKDATSK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 179 | G → Q (PubMed:3301841).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29184 Genomic DNA Translation: AAA34646.1 M29185 Genomic DNA No translation available. U55021 Genomic DNA Translation: AAB47415.1 Z75076 Genomic DNA Translation: CAA99374.1 BK006948 Genomic DNA Translation: DAA10942.1 |
PIRi | S67056, SYBYQT |
RefSeqi | NP_014811.3, NM_001183587.3 |
Genome annotation databases
EnsemblFungii | YOR168W_mRNA; YOR168W; YOR168W |
GeneIDi | 854339 |
KEGGi | sce:YOR168W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29184 Genomic DNA Translation: AAA34646.1 M29185 Genomic DNA No translation available. U55021 Genomic DNA Translation: AAB47415.1 Z75076 Genomic DNA Translation: CAA99374.1 BK006948 Genomic DNA Translation: DAA10942.1 |
PIRi | S67056, SYBYQT |
RefSeqi | NP_014811.3, NM_001183587.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3TL4 | X-ray | 2.30 | X | 1-187 | [»] | |
4H3S | X-ray | 2.15 | A | 1-809 | [»] | |
AlphaFoldDBi | P13188 | |||||
SMRi | P13188 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34564, 264 interactors |
IntActi | P13188, 1 interactor |
MINTi | P13188 |
STRINGi | 4932.YOR168W |
PTM databases
iPTMneti | P13188 |
Proteomic databases
MaxQBi | P13188 |
PaxDbi | P13188 |
PRIDEi | P13188 |
Genome annotation databases
EnsemblFungii | YOR168W_mRNA; YOR168W; YOR168W |
GeneIDi | 854339 |
KEGGi | sce:YOR168W |
Organism-specific databases
SGDi | S000005694, GLN4 |
VEuPathDBi | FungiDB:YOR168W |
Phylogenomic databases
eggNOGi | KOG1148, Eukaryota |
GeneTreei | ENSGT00940000168831 |
HOGENOMi | CLU_001882_2_3_1 |
InParanoidi | P13188 |
OMAi | INNFCAQ |
Enzyme and pathway databases
BRENDAi | 6.1.1.18, 984 6.3.5.7, 984 |
Miscellaneous databases
PROi | PR:P13188 |
RNActi | P13188, protein |
Family and domain databases
Gene3Di | 1.10.10.2420, 1 hit 1.10.8.1290, 1 hit 2.40.240.10, 2 hits 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR004514, Gln-tRNA-synth IPR007638, Gln-tRNA-synth_Ib_RNA-bd_2 IPR007639, Gln-tRNA-synth_Ib_RNA-bd_N IPR042558, Gln-tRNA-synth_Ib_RNA-bd_N_1 IPR042559, Gln-tRNA-synth_Ib_RNA-bd_N_2 IPR000924, Glu/Gln-tRNA-synth IPR020058, Glu/Gln-tRNA-synth_Ib_cat-dom IPR020059, Glu/Gln-tRNA-synth_Ib_codon-bd IPR020056, Rbsml_L25/Gln-tRNA_synth_N IPR011035, Ribosomal_L25/Gln-tRNA_synth IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749, tRNA-synt_1c, 1 hit PF03950, tRNA-synt_1c_C, 1 hit PF04558, tRNA_synt_1c_R1, 1 hit PF04557, tRNA_synt_1c_R2, 1 hit |
PRINTSi | PR00987, TRNASYNTHGLU |
SUPFAMi | SSF50715, SSF50715, 1 hit |
TIGRFAMsi | TIGR00440, glnS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYQ_YEAST | |
Accessioni | P13188Primary (citable) accession number: P13188 Secondary accession number(s): D6W2M6, Q12005 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families