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UniProtKB - P13186 (KIN2_YEAST)
Protein
Serine/threonine-protein kinase KIN2
Gene
KIN2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Serine/threonine protein kinase involved in the regulation of exocytosis. Induces phosphorylation of SEC9 and its release from the plasma membrane to the cytosol.
2 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 128 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 248 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 105 – 113 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: SGD
- protein serine kinase activity Source: RHEA
GO - Biological processi
- exocytosis Source: UniProtKB-KW
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: SGD
- positive regulation of exocytosis Source: SGD
- positive regulation of IRE1-mediated unfolded protein response Source: SGD
- protein autophosphorylation Source: SGD
- protein phosphorylation Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Exocytosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:KIN2 Ordered Locus Names:YLR096W ORF Names:L8004.3 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004086, KIN2 |
VEuPathDBi | FungiDB:YLR096W |
Subcellular locationi
Plasma membrane
Cytoplasm and Cytosol
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cellular bud neck Source: SGD
- cellular bud tip Source: SGD
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086133 | 1 – 1147 | Serine/threonine-protein kinase KIN2Add BLAST | 1147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 22 | PhosphoserineCombined sources | 1 | |
Modified residuei | 146 | PhosphoserineCombined sources | 1 | |
Modified residuei | 549 | PhosphoserineCombined sources | 1 | |
Modified residuei | 609 | PhosphoserineCombined sources | 1 | |
Modified residuei | 888 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P13186 |
PaxDbi | P13186 |
PRIDEi | P13186 |
PTM databases
iPTMneti | P13186 |
Interactioni
Subunit structurei
Interacts with SEC9 and SRO7.
1 PublicationBinary interactionsi
P13186
With | #Exp. | IntAct |
---|---|---|
BCK2 [P33306] | 4 | EBI-9723,EBI-3480 |
BUD14 [P27637] | 4 | EBI-9723,EBI-20747 |
RIM15 [P43565] | 2 | EBI-9723,EBI-15150 |
Protein-protein interaction databases
BioGRIDi | 31369, 231 interactors |
DIPi | DIP-6276N |
IntActi | P13186, 66 interactors |
MINTi | P13186 |
STRINGi | 4932.YLR096W |
Miscellaneous databases
RNActi | P13186, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 377 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 279 | |
Domaini | 1098 – 1147 | KA1PROSITE-ProRule annotationAdd BLAST | 50 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 83 | DisorderedSequence analysisAdd BLAST | 83 | |
Regioni | 572 – 592 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 629 – 687 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 730 – 749 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 860 – 882 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 1001 – 1061 | DisorderedSequence analysisAdd BLAST | 61 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 29 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 573 – 591 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 632 – 648 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 657 – 674 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000176540 |
HOGENOMi | CLU_002664_5_0_1 |
InParanoidi | P13186 |
OMAi | GGHRRKF |
Family and domain databases
InterProi | View protein in InterPro IPR028375, KA1/Ssp2_C IPR001772, KA1_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF02149, KA1, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF103243, SSF103243, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50032, KA1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P13186-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPNPNTADYL VNPNFRTSKG GSLSPTPEAF NDTRVAAPAT LRMMGKQSGP
60 70 80 90 100
RNDQQQAPLM PPADIKQGKE QAAQRQNDAS RPNGAVELRQ FHRRSLGDWE
110 120 130 140 150
FLETVGAGSM GKVKLVKHRQ TKEICVIKIV NRASKAYLHK QHSLPSPKNE
160 170 180 190 200
SEILERQKRL EKEIARDKRT VREASLGQIL YHPHICRLFE MCTMSNHFYM
210 220 230 240 250
LFEYVSGGQL LDYIIQHGSL KEHHARKFAR GIASALQYLH ANNIVHRDLK
260 270 280 290 300
IENIMISSSG EIKIIDFGLS NIFDYRKQLH TFCGSLYFAA PELLKAQPYT
310 320 330 340 350
GPEVDIWSFG IVLYVLVCGK VPFDDENSSI LHEKIKKGKV DYPSHLSIEV
360 370 380 390 400
ISLLTRMIVV DPLRRATLKN VVEHPWMNRG YDFKAPSYVP NRVPLTPEMI
410 420 430 440 450
DSQVLKEMYR LEFIDDIEDT RRSLIRLVTE KEYIQLSQEY WDKLSNAKGL
460 470 480 490 500
SSSLNNNYLN STAQQTLIQN HITSNPSQSG YNEPDSNFED PTLAYHPLLS
510 520 530 540 550
IYHLVSEMVA RKLAKLQRRQ ALALQAQAQQ RQQQQQVALG TKVALNNNSP
560 570 580 590 600
DIMTKMRSPQ KEVVPNPGIF QVPAIGTSGT SNNTNTSNKP PLHVMVPPKL
610 620 630 640 650
TIPEQAHTSP TSRKSSDIHT ELNGVLKSTP VPVSGEYQQR SASPVVGEHQ
660 670 680 690 700
EKNTIGGIFR RISQSGQSQH PTRQQEPLPE REPPTYMSKS NEISIKVPKS
710 720 730 740 750
HSRTISDYIP SARRYPSYVP NSVDVKQKPA KNTTIAPPIR SVSQKQNSDL
760 770 780 790 800
PALPQNAELI VQKQRQKLLQ ENLDKLQIND NDNNNVNAVV DGINNDNSDH
810 820 830 840 850
YLSVPKGRKL HPSARAKSVG HARRESLKFT RPPIPAALPP SDMTNDNGFL
860 870 880 890 900
GEANKERYNP VSSNFSTVPE DSTTYSNDTN NRLTSVYSQE LTEKQILEEA
910 920 930 940 950
SKAPPGSMPS IDYPKSMFLK GFFSVQTTSS KPLPIVRHNI ISVLTRMNID
960 970 980 990 1000
FKEVKGGFIC VQQRPSIETA AVPVITTTGV GLDSGKAMDL QNSLDSQLSS
1010 1020 1030 1040 1050
SYHSTASSAS RNSSIKRQGS YKRGQNNIPL TPLATNTHQR NSSIPMSPNY
1060 1070 1080 1090 1100
GNQSNGTSGE LSSMSLDYVQ QQDDILTTSR AQNINNVNGQ TEQTNTSGIK
1110 1120 1130 1140
ERPPIKFEIH IVKVRIVGLA GVHFKKVSGN TWLYKELASY ILKELNL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 216 – 217 | QH → HD in AAA34723 (PubMed:2957690).Curated | 2 | |
Sequence conflicti | 675 – 707 | QEPLP…RTISD → SGTYSSKENLQHICQNQMKF PSKYRKAIVVLYQT (PubMed:2957690).CuratedAdd BLAST | 33 | |
Sequence conflicti | 756 – 758 | NAE → KRQ in AAA34723 (PubMed:2957690).Curated | 3 | |
Sequence conflicti | 805 | P → PLSVP (PubMed:2957690).Curated | 1 | |
Sequence conflicti | 1034 – 1037 | ATNT → TTNSI (PubMed:2957690).Curated | 4 | |
Sequence conflicti | 1041 – 1042 | NS → KT in AAA34723 (PubMed:2957690).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M69018 Genomic DNA Translation: AAA34723.1 Z73268 Genomic DNA Translation: CAA97659.1 Z73269 Genomic DNA Translation: CAA97661.1 U53876 Genomic DNA Translation: AAB67540.1 BK006945 Genomic DNA Translation: DAA09412.1 |
PIRi | S64930 |
RefSeqi | NP_013197.1, NM_001181983.1 |
Genome annotation databases
EnsemblFungii | YLR096W_mRNA; YLR096W; YLR096W |
GeneIDi | 850785 |
KEGGi | sce:YLR096W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M69018 Genomic DNA Translation: AAA34723.1 Z73268 Genomic DNA Translation: CAA97659.1 Z73269 Genomic DNA Translation: CAA97661.1 U53876 Genomic DNA Translation: AAB67540.1 BK006945 Genomic DNA Translation: DAA09412.1 |
PIRi | S64930 |
RefSeqi | NP_013197.1, NM_001181983.1 |
3D structure databases
SMRi | P13186 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31369, 231 interactors |
DIPi | DIP-6276N |
IntActi | P13186, 66 interactors |
MINTi | P13186 |
STRINGi | 4932.YLR096W |
PTM databases
iPTMneti | P13186 |
Proteomic databases
MaxQBi | P13186 |
PaxDbi | P13186 |
PRIDEi | P13186 |
Genome annotation databases
EnsemblFungii | YLR096W_mRNA; YLR096W; YLR096W |
GeneIDi | 850785 |
KEGGi | sce:YLR096W |
Organism-specific databases
SGDi | S000004086, KIN2 |
VEuPathDBi | FungiDB:YLR096W |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000176540 |
HOGENOMi | CLU_002664_5_0_1 |
InParanoidi | P13186 |
OMAi | GGHRRKF |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Miscellaneous databases
PROi | PR:P13186 |
RNActi | P13186, protein |
Family and domain databases
InterProi | View protein in InterPro IPR028375, KA1/Ssp2_C IPR001772, KA1_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF02149, KA1, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF103243, SSF103243, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50032, KA1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KIN2_YEAST | |
Accessioni | P13186Primary (citable) accession number: P13186 Secondary accession number(s): D6VY96, Q12384 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | December 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 203 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - SIMILARITY comments
Index of protein domains and families