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Protein

Kexin

Gene

KEX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Processing of precursors of alpha-factors and killer toxin.

Catalytic activityi

Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors.

Cofactori

Ca2+Note: Binds 3 Ca2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi135Calcium 11
Active sitei175Charge relay system1
Metal bindingi184Calcium 11
Active sitei213Charge relay system1
Metal bindingi227Calcium 11
Metal bindingi277Calcium 21
Metal bindingi320Calcium 21
Metal bindingi350Calcium 21
Active sitei385Charge relay system1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • serine-type endopeptidase activity Source: SGD

GO - Biological processi

  • peptide pheromone maturation Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YNL238W-MONOMER
BRENDAi3.4.21.61 984

Protein family/group databases

MEROPSiS08.070

Names & Taxonomyi

Protein namesi
Recommended name:
Kexin (EC:3.4.21.61)
Alternative name(s):
Protease KEX2
Proteinase YSCF
Gene namesi
Name:KEX2
Synonyms:QDS1
Ordered Locus Names:YNL238W
ORF Names:N1122
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000005182 KEX2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini114 – 678LumenalSequence analysisAdd BLAST565
Transmembranei679 – 699HelicalSequence analysisAdd BLAST21
Topological domaini700 – 814CytoplasmicSequence analysisAdd BLAST115

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002704320 – 109Add BLAST90
PropeptideiPRO_0000027044110 – 113Removed by dipeptidylpeptidase STE134
ChainiPRO_0000027045114 – 814KexinAdd BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi163N-linked (GlcNAc...) asparagine1
Disulfide bondi230 ↔ 377
Disulfide bondi322 ↔ 352
Glycosylationi404N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi480N-linked (GlcNAc...) asparagine1

Post-translational modificationi

O-glycosylated.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP13134
PaxDbiP13134
PRIDEiP13134

PTM databases

iPTMnetiP13134

Miscellaneous databases

PMAP-CutDBiP13134

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SLA1P3279016EBI-9658,EBI-17313

Protein-protein interaction databases

BioGridi35601, 237 interactors
DIPiDIP-6287N
IntActiP13134, 15 interactors
MINTiP13134
STRINGi4932.YNL238W

Structurei

Secondary structure

1814
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13134
SMRiP13134
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13134

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini171 – 453Peptidase S8Add BLAST283
Domaini462 – 596P/Homo BPROSITE-ProRule annotationAdd BLAST135

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi625 – 642Ser/Thr-richAdd BLAST18

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00750000117358
HOGENOMiHOG000192536
InParanoidiP13134
KOiK01341
OMAiHIHTTDI
OrthoDBiEOG092C0JIG

Family and domain databases

CDDicd04059 Peptidases_S8_Protein_converta, 1 hit
Gene3Di2.60.120.260, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR034182 Kexin/furin
IPR002884 P_dom
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
PfamiView protein in Pfam
PF01483 P_proprotein, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF49785 SSF49785, 1 hit
SSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51829 P_HOMO_B, 1 hit
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13134-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVRKYITLC FWWAFSTSAL VSSQQIPLKD HTSRQYFAVE SNETLSRLEE
60 70 80 90 100
MHPNWKYEHD VRGLPNHYVF SKELLKLGKR SSLEELQGDN NDHILSVHDL
110 120 130 140 150
FPRNDLFKRL PVPAPPMDSS LLPVKEAEDK LSINDPLFER QWHLVNPSFP
160 170 180 190 200
GSDINVLDLW YNNITGAGVV AAIVDDGLDY ENEDLKDNFC AEGSWDFNDN
210 220 230 240 250
TNLPKPRLSD DYHGTRCAGE IAAKKGNNFC GVGVGYNAKI SGIRILSGDI
260 270 280 290 300
TTEDEAASLI YGLDVNDIYS CSWGPADDGR HLQGPSDLVK KALVKGVTEG
310 320 330 340 350
RDSKGAIYVF ASGNGGTRGD NCNYDGYTNS IYSITIGAID HKDLHPPYSE
360 370 380 390 400
GCSAVMAVTY SSGSGEYIHS SDINGRCSNS HGGTSAAAPL AAGVYTLLLE
410 420 430 440 450
ANPNLTWRDV QYLSILSAVG LEKNADGDWR DSAMGKKYSH RYGFGKIDAH
460 470 480 490 500
KLIEMSKTWE NVNAQTWFYL PTLYVSQSTN STEETLESVI TISEKSLQDA
510 520 530 540 550
NFKRIEHVTV TVDIDTEIRG TTTVDLISPA GIISNLGVVR PRDVSSEGFK
560 570 580 590 600
DWTFMSVAHW GENGVGDWKI KVKTTENGHR IDFHSWRLKL FGESIDSSKT
610 620 630 640 650
ETFVFGNDKE EVEPAATEST VSQYSASSTS ISISATSTSS ISIGVETSAI
660 670 680 690 700
PQTTTASTDP DSDPNTPKKL SSPRQAMHYF LTIFLIGATF LVLYFMFFMK
710 720 730 740 750
SRRRIRRSRA ETYEFDIIDT DSEYDSTLDN GTSGITEPEE VEDFDFDLSD
760 770 780 790 800
EDHLASLSSS ENGDAEHTID SVLTNENPFS DPIKQKFPND ANAESASNKL
810
QELQPDVPPS SGRS
Length:814
Mass (Da):90,003
Last modified:January 1, 1990 - v1
Checksum:i5DCEEDADCF439C78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22870 Genomic DNA Translation: AAA34719.1
M24201 Genomic DNA Translation: AAA34718.1
Z69381 Genomic DNA Translation: CAA93360.1
Z71514 Genomic DNA Translation: CAA96143.1
BK006947 Genomic DNA Translation: DAA10321.1
PIRiA28931 KXBY
RefSeqiNP_014161.1, NM_001183076.1

Genome annotation databases

EnsemblFungiiYNL238W; YNL238W; YNL238W
GeneIDi855483
KEGGisce:YNL238W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22870 Genomic DNA Translation: AAA34719.1
M24201 Genomic DNA Translation: AAA34718.1
Z69381 Genomic DNA Translation: CAA93360.1
Z71514 Genomic DNA Translation: CAA96143.1
BK006947 Genomic DNA Translation: DAA10321.1
PIRiA28931 KXBY
RefSeqiNP_014161.1, NM_001183076.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OT5X-ray2.40A/B123-599[»]
1R64X-ray2.20A/B121-601[»]
2ID4X-ray1.90A/B114-613[»]
ProteinModelPortaliP13134
SMRiP13134
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35601, 237 interactors
DIPiDIP-6287N
IntActiP13134, 15 interactors
MINTiP13134
STRINGi4932.YNL238W

Protein family/group databases

MEROPSiS08.070

PTM databases

iPTMnetiP13134

Proteomic databases

MaxQBiP13134
PaxDbiP13134
PRIDEiP13134

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL238W; YNL238W; YNL238W
GeneIDi855483
KEGGisce:YNL238W

Organism-specific databases

SGDiS000005182 KEX2

Phylogenomic databases

GeneTreeiENSGT00750000117358
HOGENOMiHOG000192536
InParanoidiP13134
KOiK01341
OMAiHIHTTDI
OrthoDBiEOG092C0JIG

Enzyme and pathway databases

BioCyciYEAST:YNL238W-MONOMER
BRENDAi3.4.21.61 984

Miscellaneous databases

EvolutionaryTraceiP13134
PMAP-CutDBiP13134
PROiPR:P13134

Family and domain databases

CDDicd04059 Peptidases_S8_Protein_converta, 1 hit
Gene3Di2.60.120.260, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR034182 Kexin/furin
IPR002884 P_dom
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
PfamiView protein in Pfam
PF01483 P_proprotein, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF49785 SSF49785, 1 hit
SSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51829 P_HOMO_B, 1 hit
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKEX2_YEAST
AccessioniPrimary (citable) accession number: P13134
Secondary accession number(s): D6W0V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 7, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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