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UniProtKB - P13134 (KEX2_YEAST)
Protein
Kexin
Gene
KEX2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Processing of precursors of alpha-factors and killer toxin.
Catalytic activityi
- Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors. EC:3.4.21.61
Cofactori
Ca2+Note: Binds 3 Ca2+ ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 135 | Calcium 1 | 1 | |
Active sitei | 175 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Metal bindingi | 184 | Calcium 1 | 1 | |
Active sitei | 213 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Metal bindingi | 227 | Calcium 1 | 1 | |
Metal bindingi | 277 | Calcium 2 | 1 | |
Metal bindingi | 320 | Calcium 2 | 1 | |
Metal bindingi | 350 | Calcium 2 | 1 | |
Active sitei | 385 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- serine-type endopeptidase activity Source: SGD
GO - Biological processi
- peptide pheromone maturation Source: SGD
- protein processing Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.21.61, 984 |
Protein family/group databases
MEROPSi | S08.070 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:KEX2 Synonyms:QDS1 Ordered Locus Names:YNL238W ORF Names:N1122 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005182, KEX2 |
VEuPathDBi | FungiDB:YNL238W |
Subcellular locationi
Golgi apparatus
Golgi apparatus
- integral component of Golgi membrane Source: GO_Central
- trans-Golgi network Source: SGD
Vacuole
- fungal-type vacuole Source: SGD
Other locations
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 114 – 678 | LumenalSequence analysisAdd BLAST | 565 | |
Transmembranei | 679 – 699 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 700 – 814 | CytoplasmicSequence analysisAdd BLAST | 115 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000027043 | 20 – 109 | Add BLAST | 90 | |
PropeptideiPRO_0000027044 | 110 – 113 | Removed by dipeptidylpeptidase STE13 | 4 | |
ChainiPRO_0000027045 | 114 – 814 | KexinAdd BLAST | 701 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 42 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 163 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 230 ↔ 377 | |||
Disulfide bondi | 322 ↔ 352 | |||
Glycosylationi | 404 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 480 | N-linked (GlcNAc...) asparagine | 1 |
Post-translational modificationi
O-glycosylated.
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
MaxQBi | P13134 |
PaxDbi | P13134 |
PRIDEi | P13134 |
PTM databases
iPTMneti | P13134 |
Interactioni
Binary interactionsi
P13134
With | #Exp. | IntAct |
---|---|---|
SLA1 [P32790] | 16 | EBI-9658,EBI-17313 |
Protein-protein interaction databases
BioGRIDi | 35601, 239 interactors |
DIPi | DIP-6287N |
IntActi | P13134, 15 interactors |
MINTi | P13134 |
STRINGi | 4932.YNL238W |
Miscellaneous databases
RNActi | P13134, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P13134 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13134 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 141 – 453 | Peptidase S8PROSITE-ProRule annotationAdd BLAST | 313 | |
Domaini | 462 – 596 | P/Homo BPROSITE-ProRule annotationAdd BLAST | 135 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 651 – 671 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 756 – 814 | DisorderedSequence analysisAdd BLAST | 59 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 756 – 782 | Polar residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 790 – 814 | Polar residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3525, Eukaryota |
GeneTreei | ENSGT00940000167869 |
HOGENOMi | CLU_002976_2_1_1 |
InParanoidi | P13134 |
OMAi | HIHTTDI |
Family and domain databases
CDDi | cd04059, Peptidases_S8_Protein_convertases_Kexins_Furin-like, 1 hit |
Gene3Di | 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR034182, Kexin/furin IPR002884, P_dom IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel |
Pfami | View protein in Pfam PF01483, P_proprotein, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51829, P_HOMO_B, 1 hit PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P13134-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVRKYITLC FWWAFSTSAL VSSQQIPLKD HTSRQYFAVE SNETLSRLEE
60 70 80 90 100
MHPNWKYEHD VRGLPNHYVF SKELLKLGKR SSLEELQGDN NDHILSVHDL
110 120 130 140 150
FPRNDLFKRL PVPAPPMDSS LLPVKEAEDK LSINDPLFER QWHLVNPSFP
160 170 180 190 200
GSDINVLDLW YNNITGAGVV AAIVDDGLDY ENEDLKDNFC AEGSWDFNDN
210 220 230 240 250
TNLPKPRLSD DYHGTRCAGE IAAKKGNNFC GVGVGYNAKI SGIRILSGDI
260 270 280 290 300
TTEDEAASLI YGLDVNDIYS CSWGPADDGR HLQGPSDLVK KALVKGVTEG
310 320 330 340 350
RDSKGAIYVF ASGNGGTRGD NCNYDGYTNS IYSITIGAID HKDLHPPYSE
360 370 380 390 400
GCSAVMAVTY SSGSGEYIHS SDINGRCSNS HGGTSAAAPL AAGVYTLLLE
410 420 430 440 450
ANPNLTWRDV QYLSILSAVG LEKNADGDWR DSAMGKKYSH RYGFGKIDAH
460 470 480 490 500
KLIEMSKTWE NVNAQTWFYL PTLYVSQSTN STEETLESVI TISEKSLQDA
510 520 530 540 550
NFKRIEHVTV TVDIDTEIRG TTTVDLISPA GIISNLGVVR PRDVSSEGFK
560 570 580 590 600
DWTFMSVAHW GENGVGDWKI KVKTTENGHR IDFHSWRLKL FGESIDSSKT
610 620 630 640 650
ETFVFGNDKE EVEPAATEST VSQYSASSTS ISISATSTSS ISIGVETSAI
660 670 680 690 700
PQTTTASTDP DSDPNTPKKL SSPRQAMHYF LTIFLIGATF LVLYFMFFMK
710 720 730 740 750
SRRRIRRSRA ETYEFDIIDT DSEYDSTLDN GTSGITEPEE VEDFDFDLSD
760 770 780 790 800
EDHLASLSSS ENGDAEHTID SVLTNENPFS DPIKQKFPND ANAESASNKL
810
QELQPDVPPS SGRS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22870 Genomic DNA Translation: AAA34719.1 M24201 Genomic DNA Translation: AAA34718.1 Z69381 Genomic DNA Translation: CAA93360.1 Z71514 Genomic DNA Translation: CAA96143.1 BK006947 Genomic DNA Translation: DAA10321.1 |
PIRi | A28931, KXBY |
RefSeqi | NP_014161.1, NM_001183076.1 |
Genome annotation databases
EnsemblFungii | YNL238W_mRNA; YNL238W; YNL238W |
GeneIDi | 855483 |
KEGGi | sce:YNL238W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22870 Genomic DNA Translation: AAA34719.1 M24201 Genomic DNA Translation: AAA34718.1 Z69381 Genomic DNA Translation: CAA93360.1 Z71514 Genomic DNA Translation: CAA96143.1 BK006947 Genomic DNA Translation: DAA10321.1 |
PIRi | A28931, KXBY |
RefSeqi | NP_014161.1, NM_001183076.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OT5 | X-ray | 2.40 | A/B | 123-599 | [»] | |
1R64 | X-ray | 2.20 | A/B | 121-601 | [»] | |
2ID4 | X-ray | 1.90 | A/B | 114-613 | [»] | |
SMRi | P13134 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35601, 239 interactors |
DIPi | DIP-6287N |
IntActi | P13134, 15 interactors |
MINTi | P13134 |
STRINGi | 4932.YNL238W |
Protein family/group databases
MEROPSi | S08.070 |
PTM databases
iPTMneti | P13134 |
Proteomic databases
MaxQBi | P13134 |
PaxDbi | P13134 |
PRIDEi | P13134 |
Genome annotation databases
EnsemblFungii | YNL238W_mRNA; YNL238W; YNL238W |
GeneIDi | 855483 |
KEGGi | sce:YNL238W |
Organism-specific databases
SGDi | S000005182, KEX2 |
VEuPathDBi | FungiDB:YNL238W |
Phylogenomic databases
eggNOGi | KOG3525, Eukaryota |
GeneTreei | ENSGT00940000167869 |
HOGENOMi | CLU_002976_2_1_1 |
InParanoidi | P13134 |
OMAi | HIHTTDI |
Enzyme and pathway databases
BRENDAi | 3.4.21.61, 984 |
Miscellaneous databases
EvolutionaryTracei | P13134 |
PROi | PR:P13134 |
RNActi | P13134, protein |
Family and domain databases
CDDi | cd04059, Peptidases_S8_Protein_convertases_Kexins_Furin-like, 1 hit |
Gene3Di | 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR034182, Kexin/furin IPR002884, P_dom IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel |
Pfami | View protein in Pfam PF01483, P_proprotein, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51829, P_HOMO_B, 1 hit PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KEX2_YEAST | |
Accessioni | P13134Primary (citable) accession number: P13134 Secondary accession number(s): D6W0V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 207 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families