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UniProtKB - P13114 (CHSY_ARATH)
Protein
Chalcone synthase
Gene
CHS
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activityi
- 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoAPROSITE-ProRule annotationEC:2.3.1.74PROSITE-ProRule annotation
: flavonoid biosynthesis Pathwayi
This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 169 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- acyltransferase activity, transferring groups other than amino-acyl groups Source: GO_Central
- chalcone synthase activity Source: UniProtKB-EC
- naringenin-chalcone synthase activity Source: UniProtKB-EC
GO - Biological processi
- auxin polar transport Source: TAIR
- flavonoid biosynthetic process Source: TAIR
- polyketide biosynthetic process Source: GO_Central
- regulation of anthocyanin biosynthetic process Source: TAIR
- response to auxin Source: TAIR
- response to gravity Source: TAIR
- response to jasmonic acid Source: TAIR
- response to oxidative stress Source: TAIR
- response to UV-B Source: TAIR
- response to wounding Source: TAIR
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Flavonoid biosynthesis |
Enzyme and pathway databases
BioCyci | ARA:AT5G13930-MONOMER |
BRENDAi | 2.3.1.74, 399 |
UniPathwayi | UPA00154 |
Names & Taxonomyi
Protein namesi | Recommended name: Chalcone synthase (EC:2.3.1.74)Alternative name(s): Naringenin-chalcone synthase Protein TRANSPARENT TESTA 4 |
Gene namesi | Name:CHS Synonyms:TT4 Ordered Locus Names:At5g13930 ORF Names:MAC12.11, MAC12.14 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G13930 |
TAIRi | locus:2159098, AT5G13930 |
Subcellular locationi
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
Nucleus
- nucleus Source: TAIR
Vacuole
- plant-type vacuole membrane Source: TAIR
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 309 | H → Y in JK196. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | ||||
ChainiPRO_0000215954 | 2 – 395 | Chalcone synthaseAdd BLAST | 394 | ||
Isoform 2 (identifier: P13114-2) | |||||
Initiator methioninei | RemovedCombined sources |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 2 | N-acetylvalineCombined sources | 1 | ||
Isoform 2 (identifier: P13114-2) | |||||
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | P13114 |
PRIDEi | P13114 |
ProteomicsDBi | 246835 [P13114-1] |
PTM databases
iPTMneti | P13114 |
Expressioni
Gene expression databases
ExpressionAtlasi | P13114, baseline and differential |
Genevisiblei | P13114, AT |
Interactioni
Binary interactionsi
P13114
With | #Exp. | IntAct |
---|---|---|
ARF18 [Q9C5W9] | 3 | EBI-1546775,EBI-3946783 |
CHI1 [P41088] | 4 | EBI-1546775,EBI-1546761 |
DFRA [P51102] | 4 | EBI-1546775,EBI-1546795 |
FLS1 [Q96330] | 3 | EBI-1546775,EBI-1774454 |
Protein-protein interaction databases
BioGRIDi | 16519, 11 interactors |
IntActi | P13114, 8 interactors |
MINTi | P13114 |
STRINGi | 3702.AT5G13930.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P13114 |
SMRi | P13114 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S4A6, Eukaryota |
HOGENOMi | CLU_034992_2_0_1 |
InParanoidi | P13114 |
OMAi | NFMGCYA |
PhylomeDBi | P13114 |
Family and domain databases
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR012328, Chalcone/stilbene_synt_C IPR001099, Chalcone/stilbene_synt_N IPR018088, Chalcone/stilbene_synthase_AS IPR011141, Polyketide_synthase_type-III IPR016039, Thiolase-like |
PANTHERi | PTHR11877, PTHR11877, 1 hit |
Pfami | View protein in Pfam PF02797, Chal_sti_synt_C, 1 hit PF00195, Chal_sti_synt_N, 1 hit |
PIRSFi | PIRSF000451, PKS_III, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
PROSITEi | View protein in PROSITE PS00441, CHALCONE_SYNTH, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketIsoform 1 (identifier: P13114-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVMAGASSLD EIRQAQRADG PAGILAIGTA NPENHVLQAE YPDYYFRITN
60 70 80 90 100
SEHMTDLKEK FKRMCDKSTI RKRHMHLTEE FLKENPHMCA YMAPSLDTRQ
110 120 130 140 150
DIVVVEVPKL GKEAAVKAIK EWGQPKSKIT HVVFCTTSGV DMPGADYQLT
160 170 180 190 200
KLLGLRPSVK RLMMYQQGCF AGGTVLRIAK DLAENNRGAR VLVVCSEITA
210 220 230 240 250
VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK PIFEMVSAAQ
260 270 280 290 300
TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIVKSLD EAFKPLGISD
310 320 330 340 350
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVLSEY GNMSSACVLF
360 370 380 390
ILDEMRRKSA KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | P → H in AAL06937 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 54 | M → K in AAL06937 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 268 | G → S in AAB35812 (PubMed:8528278).Curated | 1 | |
Sequence conflicti | 376 | L → S in AAL06937 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 384 | T → S in AAL06937 (PubMed:14593172).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057945 | 1 – 2 | Missing in isoform 2. Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20308 Genomic DNA Translation: AAA32771.1 S80554 Genomic DNA Translation: AAB35812.1 Y18602 Genomic DNA Translation: CAC80089.1 Y18603 Genomic DNA Translation: CAC80090.1 AF112086 Genomic DNA Translation: AAF23561.1 AB005230 Genomic DNA Translation: BAB11121.1 CP002688 Genomic DNA Translation: AED91961.1 AY044331 mRNA Translation: AAK73272.1 AY054278 mRNA Translation: AAL06937.1 AY058155 mRNA Translation: AAL25571.1 AY090376 mRNA Translation: AAL91279.1 AY087778 mRNA Translation: AAM65314.1 BT000596 mRNA Translation: AAN18165.1 Z17650 mRNA Translation: CAA79023.1 Z17649 mRNA Translation: CAA79022.1 |
PIRi | A27721, SYMUCN |
RefSeqi | NP_196897.1, NM_121396.4 [P13114-1] |
Genome annotation databases
EnsemblPlantsi | AT5G13930.1; AT5G13930.1; AT5G13930 [P13114-1] |
GeneIDi | 831241 |
Gramenei | AT5G13930.1; AT5G13930.1; AT5G13930 [P13114-1] |
KEGGi | ath:AT5G13930 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20308 Genomic DNA Translation: AAA32771.1 S80554 Genomic DNA Translation: AAB35812.1 Y18602 Genomic DNA Translation: CAC80089.1 Y18603 Genomic DNA Translation: CAC80090.1 AF112086 Genomic DNA Translation: AAF23561.1 AB005230 Genomic DNA Translation: BAB11121.1 CP002688 Genomic DNA Translation: AED91961.1 AY044331 mRNA Translation: AAK73272.1 AY054278 mRNA Translation: AAL06937.1 AY058155 mRNA Translation: AAL25571.1 AY090376 mRNA Translation: AAL91279.1 AY087778 mRNA Translation: AAM65314.1 BT000596 mRNA Translation: AAN18165.1 Z17650 mRNA Translation: CAA79023.1 Z17649 mRNA Translation: CAA79022.1 |
PIRi | A27721, SYMUCN |
RefSeqi | NP_196897.1, NM_121396.4 [P13114-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6DXB | X-ray | 1.55 | A/B/C/D | 1-395 | [»] | |
6DXD | X-ray | 1.59 | A/B/C/D | 1-395 | [»] | |
6DXE | X-ray | 1.61 | A/C | 1-395 | [»] | |
AlphaFoldDBi | P13114 | |||||
SMRi | P13114 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 16519, 11 interactors |
IntActi | P13114, 8 interactors |
MINTi | P13114 |
STRINGi | 3702.AT5G13930.1 |
PTM databases
iPTMneti | P13114 |
Proteomic databases
PaxDbi | P13114 |
PRIDEi | P13114 |
ProteomicsDBi | 246835 [P13114-1] |
Genome annotation databases
EnsemblPlantsi | AT5G13930.1; AT5G13930.1; AT5G13930 [P13114-1] |
GeneIDi | 831241 |
Gramenei | AT5G13930.1; AT5G13930.1; AT5G13930 [P13114-1] |
KEGGi | ath:AT5G13930 |
Organism-specific databases
Araporti | AT5G13930 |
TAIRi | locus:2159098, AT5G13930 |
Phylogenomic databases
eggNOGi | ENOG502S4A6, Eukaryota |
HOGENOMi | CLU_034992_2_0_1 |
InParanoidi | P13114 |
OMAi | NFMGCYA |
PhylomeDBi | P13114 |
Enzyme and pathway databases
UniPathwayi | UPA00154 |
BioCyci | ARA:AT5G13930-MONOMER |
BRENDAi | 2.3.1.74, 399 |
Miscellaneous databases
PROi | PR:P13114 |
Gene expression databases
ExpressionAtlasi | P13114, baseline and differential |
Genevisiblei | P13114, AT |
Family and domain databases
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR012328, Chalcone/stilbene_synt_C IPR001099, Chalcone/stilbene_synt_N IPR018088, Chalcone/stilbene_synthase_AS IPR011141, Polyketide_synthase_type-III IPR016039, Thiolase-like |
PANTHERi | PTHR11877, PTHR11877, 1 hit |
Pfami | View protein in Pfam PF02797, Chal_sti_synt_C, 1 hit PF00195, Chal_sti_synt_N, 1 hit |
PIRSFi | PIRSF000451, PKS_III, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
PROSITEi | View protein in PROSITE PS00441, CHALCONE_SYNTH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHSY_ARATH | |
Accessioni | P13114Primary (citable) accession number: P13114 Secondary accession number(s): Q43132, Q940L6, Q941R9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families