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Protein

Enhancer of split m8 protein

Gene

E(spl)

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the control of cell fate choice by uncommitted neuroectodermal cells in the embryo (PubMed:2540957). Transcriptional repressor (PubMed:8001118). Binds DNA on N-box motifs: 5'-CACNAG-3' (PubMed:8001118). Part of the Notch signaling pathway (PubMed:22357926).3 Publications

GO - Molecular functioni

  • DNA binding transcription factor activity Source: FlyBase
  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • compound eye development Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • ectoderm development Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • mesoderm development Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • regulation of R8 cell spacing in compound eye Source: FlyBase
  • sensory organ precursor cell fate determination Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of split m8 protein
Short name:
E(spl)m8
Gene namesi
Name:E(spl)
Synonyms:m8
ORF Names:CG8365
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000591 E(spl)

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271751 – 179Enhancer of split m8 proteinAdd BLAST179

Proteomic databases

PaxDbiP13098
PRIDEiP13098

PTM databases

iPTMnetiP13098

Expressioni

Developmental stagei

In larvae, detected in neuroblasts (at protein level) (PubMed:22357926). Mesectodermal expression appears shortly before the onset of gastrulation (PubMed:2540957). In imaginal disks, expression is seen primarily within presumptive proneural clusters of eye-antennal, wing and leg disks (PubMed:2540957).2 Publications

Gene expression databases

BgeeiFBgn0000591
GenevisibleiP13098 DM

Interactioni

Subunit structurei

Homodimer (PubMed:22357926). Heterodimers with dpn (PubMed:22357926).Transcription repression requires formation of a complex with a corepressor protein (Groucho) (PubMed:8001118).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi68061, 34 interactors
DIPiDIP-22511N
ELMiP13098
IntActiP13098, 14 interactors
MINTiP13098
STRINGi7227.FBpp0084335

Structurei

3D structure databases

ProteinModelPortaliP13098
SMRiP13098
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 65bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini83 – 116OrangePROSITE-ProRule annotationAdd BLAST34

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 179WRPW motif4

Domaini

The orange domain and the basic helix-loop-helix motif mediate repression of specific transcriptional activators, such as basic helix-loop-helix protein dimers.1 Publication
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.1 Publication

Phylogenomic databases

eggNOGiKOG4304 Eukaryota
ENOG4111F0X LUCA
InParanoidiP13098
KOiK09090
OMAiGRVYKNL
OrthoDBiEOG091G0NI7
PhylomeDBiP13098

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003650 Orange_dom
PfamiView protein in Pfam
PF07527 Hairy_orange, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00511 ORANGE, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS51054 ORANGE, 1 hit

Sequencei

Sequence statusi: Complete.

P13098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYTTKTQIY QKVKKPMLER QRRARMNKCL DNLKTLVAEL RGDDGILRMD
60 70 80 90 100
KAEMLESAVI FMRQQKTPKK VAQEEQSLPL DSFKNGYMNA VNEVSRVMAS
110 120 130 140 150
TPGMSVDLGK SVMTHLGRVY KNLQQFHEAQ SAADFIQNSM DCSSMDKAPL
160 170
SPASSGYHSD CDSPAPSPQP MQQPLWRPW
Length:179
Mass (Da):20,303
Last modified:December 1, 2000 - v2
Checksum:i017B139DE0C328B4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165 – 167APS → PPT in CAA34554 (PubMed:2540957).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti114T → I in strain: NVIII-2, NVIII-5 and NVIII-m15. 1 Publication1
Natural varianti133A → T in strain: NVIII-m11 and NVIII-m19. 1 Publication1
Natural varianti134D → N in strain: NVIII-m19. 1 Publication1
Natural varianti141D → V in strain: NVIII-m19. 1 Publication1
Natural varianti174P → T in strain: NVIII-m12. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16553 Genomic DNA Translation: CAA34554.1
AY779906 Genomic DNA Translation: AAV59051.1
AY779907 Genomic DNA Translation: AAV59063.1
AY779908 Genomic DNA Translation: AAV59075.1
AY779909 Genomic DNA Translation: AAV59087.1
AY779910 Genomic DNA Translation: AAV59099.1
AY779911 Genomic DNA Translation: AAV59111.1
AY779912 Genomic DNA Translation: AAV59123.1
AY779913 Genomic DNA Translation: AAV59135.1
AY779914 Genomic DNA Translation: AAV59147.1
AY779915 Genomic DNA Translation: AAV59159.1
AY779916 Genomic DNA Translation: AAV59171.1
AY779917 Genomic DNA Translation: AAV59183.1
AY779918 Genomic DNA Translation: AAV59195.1
AY779919 Genomic DNA Translation: AAV59207.1
AY779920 Genomic DNA Translation: AAV59219.1
AY779921 Genomic DNA Translation: AAV59231.1
AE014297 Genomic DNA Translation: AAF56555.1
BT028829 mRNA Translation: ABI34210.1
BT028834 mRNA Translation: ABI34215.2
BT028861 mRNA Translation: ABI34242.1
BT028867 mRNA Translation: ABI34248.2
DQ138662 Genomic DNA Translation: ABA86268.1
PIRiS03627
RefSeqiNP_524513.1, NM_079789.3
UniGeneiDm.27416

Genome annotation databases

EnsemblMetazoaiFBtr0084961; FBpp0084335; FBgn0000591
GeneIDi43161
KEGGidme:Dmel_CG8365

Similar proteinsi

Entry informationi

Entry nameiESM8_DROME
AccessioniPrimary (citable) accession number: P13098
Secondary accession number(s): Q0IGQ3
, Q2XYK0, Q5S426, Q5S462, Q5S474, Q5S4H0, Q5S4I2, Q9VBI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 2000
Last modified: July 18, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase

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