Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lantibiotic nisin-A

Gene

spaN

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Miscellaneous

In strain 6F3 the nisA gene is encoded on an unnamed plasmid. The reported protein sequences are identical.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Bacteriocin, Lantibiotic

Protein family/group databases

TCDBi1.C.20.1.1 the nisin (nisin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Lantibiotic nisin-A
Gene namesi
Name:spaN
Synonyms:nisA
Encoded oniPlasmid unnamed1 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Biotechnological usei

Used as a food preservative.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000171221 – 232 PublicationsAdd BLAST23
PeptideiPRO_000001712324 – 57Lantibiotic nisin-AAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25(Z)-2,3-didehydrobutyrine1 Publication1
Cross-linki26 ↔ 30Lanthionine (Ser-Cys)1 Publication
Modified residuei282,3-didehydroalanine (Ser)1 Publication1
Cross-linki31 ↔ 34Beta-methyllanthionine (Thr-Cys)1 Publication
Cross-linki36 ↔ 42Beta-methyllanthionine (Thr-Cys)1 Publication
Cross-linki46 ↔ 49Beta-methyllanthionine (Thr-Cys)1 Publication
Cross-linki48 ↔ 51Beta-methyllanthionine (Thr-Cys)1 Publication
Modified residuei562,3-didehydroalanine (Ser)1 Publication1

Post-translational modificationi

Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
The structure of the 2,3-didehydrobutyrine is not discussed in PubMed:8454055. However, in Fig. 1 the residue is diagrammed as the Z-isomer.

Keywords - PTMi

Thioether bond

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP13068
SMRiP13068
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type A lantibiotic family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR006079 Lantibiotic_typ-A_Bacillales
PfamiView protein in Pfam
PF02052 Gallidermin, 1 hit
PRINTSiPR00324 NISIN
TIGRFAMsiTIGR03731 lantibio_gallid, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13068-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTKDFNLDL VSVSKKDSGA SPRITSISLC TPGCKTGALM GCNMKTATCH

CSIHVSK
Length:57
Mass (Da):5,963
Last modified:January 1, 1990 - v1
Checksum:i315E4428AC70BFEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04057 Genomic DNA Translation: AAA88606.1
M65089 Genomic DNA Translation: AAA73038.1
M24527 Genomic DNA Translation: AAA26948.1
X68307 Genomic DNA Translation: CAA48380.1
M27277 Genomic DNA Translation: AAA25188.1
D00696 Genomic DNA Translation: BAA00602.1
L16226 Genomic DNA Translation: AAA25189.1
M79445 Genomic DNA Translation: AAA25198.1
PIRiA31915 NILLSA
RefSeqiWP_014570405.1, NZ_LKLX01000138.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04057 Genomic DNA Translation: AAA88606.1
M65089 Genomic DNA Translation: AAA73038.1
M24527 Genomic DNA Translation: AAA26948.1
X68307 Genomic DNA Translation: CAA48380.1
M27277 Genomic DNA Translation: AAA25188.1
D00696 Genomic DNA Translation: BAA00602.1
L16226 Genomic DNA Translation: AAA25189.1
M79445 Genomic DNA Translation: AAA25198.1
PIRiA31915 NILLSA
RefSeqiWP_014570405.1, NZ_LKLX01000138.1

3D structure databases

ProteinModelPortaliP13068
SMRiP13068
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.20.1.1 the nisin (nisin) family

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR006079 Lantibiotic_typ-A_Bacillales
PfamiView protein in Pfam
PF02052 Gallidermin, 1 hit
PRINTSiPR00324 NISIN
TIGRFAMsiTIGR03731 lantibio_gallid, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLANN_LACLL
AccessioniPrimary (citable) accession number: P13068
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again