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Protein

Elongation factor 2

Gene

EF2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33GTPBy similarity8
Nucleotide bindingi108 – 112GTPBy similarity5
Nucleotide bindingi162 – 165GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: FlyBase

GO - Biological processi

  • translational elongation Source: FlyBase

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-156902 Peptide chain elongation
R-DME-5358493 Synthesis of diphthamide-EEF2
R-DME-6798695 Neutrophil degranulation
R-DME-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:EF2
Synonyms:Ef2b
ORF Names:CG2238
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000559 EF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000910122 – 844Elongation factor 2Add BLAST843

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphothreonineBy similarity1
Modified residuei59PhosphothreonineBy similarity1
Modified residuei701DiphthamideBy similarity1

Post-translational modificationi

Phosphorylation by EF-2 kinase completely inactivates EF-2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13060
PRIDEiP13060

Expressioni

Developmental stagei

Expression commences during embryonic germ band elongation and persists throughout development and in the adult. Highest level of expression observed in late embryonic, late larval and early pupal stages.1 Publication

Gene expression databases

BgeeiFBgn0000559
GenevisibleiP13060 DM

Interactioni

Protein-protein interaction databases

BioGridi61367, 74 interactors
DIPiDIP-19881N
IntActiP13060, 39 interactors
MINTiP13060
STRINGi7227.FBpp0305182

Structurei

3D structure databases

ProteinModelPortaliP13060
SMRiP13060
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 348tr-type GPROSITE-ProRule annotationAdd BLAST332

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0469 Eukaryota
COG0480 LUCA
GeneTreeiENSGT00900000141114
HOGENOMiHOG000248060
InParanoidiP13060
KOiK03234
OMAiEHLISGM
OrthoDBiEOG091G0A2J
PhylomeDBiP13060

Family and domain databases

Gene3Di3.30.230.10, 2 hits
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNFTVDEIR GLMDKKRNIR NMSVIAHVDH GKSTLTDSLV SKAGIIAGAK
60 70 80 90 100
AGETRFTDTR KDEQERCITI KSTAISMYFE VEEKDLVFIT HPDQREKECK
110 120 130 140 150
GFLINLIDSP GHVDFSSEVT AALRVTDGAL VVVDCVSGVC VQTETVLRQA
160 170 180 190 200
IAERIKPILF MNKMDRALLE LQLDAEELYQ TFQRIVENVN VIIATYNDDG
210 220 230 240 250
GPMGEVRVDP SKGSVGFGSG LHGWAFTLKQ FSEMYSEKFK IDVVKLMNRL
260 270 280 290 300
WGENFFNAKT KKWQKQKEAD NKRSFCMYIL DPIYKVFDAI MNYKKEEIGT
310 320 330 340 350
LLEKIGVTLK HEDKDKDGKA LLKTVMRTWL PAGEALLQMI AIHLPSPVVA
360 370 380 390 400
QKYRMEMLYE GPHDDEAAIA VKSCDPDGPL MMYISKMVPT SDKGRFYAFG
410 420 430 440 450
RVFAGKVATG QKCRIMGPNY TPGKKEDLYE KAIQRTILMM GRYVEAIEDV
460 470 480 490 500
PSGNICGLVG VDQFLVKTGT ITTFKDAHNM KVMKFSVSPV VRVAVEPKNP
510 520 530 540 550
ADLPKLVEGL KRLAKSDPMV QCIIEESGEH IIAGAGELHL EICLKDLEED
560 570 580 590 600
HACIPLKKSD PVVSYRETVS EESDQMCLSK SPNKHNRLLM KALPMPDGLP
610 620 630 640 650
EDIDNGDVSA KDEFKARARY LSEKYDYDVT EARKIWCFGP DGTGPNFILD
660 670 680 690 700
CTKSVQYLNE IKDSVVAGFQ WASKEGILAD ENLRGVRFNI YDVTLHADAI
710 720 730 740 750
HRGGGQIIPT TRRCLYAAAI TAKPRLMEPV YLCEIQCPEV AVGGIYGVLN
760 770 780 790 800
RRRGHVFEEN QVVGTPMFVV KAYLPVNESF GFTADLRSNT GGQAFPQCVF
810 820 830 840
DHWQVLPGDP SEPSSKPYAI VQDTRKRKGL KEGLPDLSQY LDKL
Length:844
Mass (Da):94,459
Last modified:January 23, 2007 - v4
Checksum:i547A6ED0658B6B6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti607D → E in CAA33804 (PubMed:2508059).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15805 mRNA Translation: CAA33804.1
AE014134 Genomic DNA Translation: AAF57226.2
AE014134 Genomic DNA Translation: AAG22125.3
AE014134 Genomic DNA Translation: AAN11135.2
AY075481 mRNA Translation: AAL68292.1
PIRiS05988
RefSeqiNP_525105.2, NM_080366.3
NP_724357.2, NM_165394.3
NP_724358.2, NM_165395.2
UniGeneiDm.6600

Genome annotation databases

EnsemblMetazoaiFBtr0085911; FBpp0085265; FBgn0000559
FBtr0085912; FBpp0085266; FBgn0000559
FBtr0332966; FBpp0305182; FBgn0000559
GeneIDi35422
KEGGidme:Dmel_CG2238

Similar proteinsi

Entry informationi

Entry nameiEF2_DROME
AccessioniPrimary (citable) accession number: P13060
Secondary accession number(s): A0A023GPJ5, Q9I7H2, Q9V9R0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 181 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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