UniProtKB - P13045 (GAL3_YEAST)
Protein
Protein GAL3
Gene
GAL3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.
Miscellaneous
Present with 721 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- galactokinase activity Source: GO_Central
GO - Biological processi
- galactose metabolic process Source: SGD
- maintenance of protein location Source: SGD
- positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
Keywordsi
Biological process | Carbohydrate metabolism, Galactose metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Protein GAL3 |
Gene namesi | Name:GAL3 Ordered Locus Names:YDR009W ORF Names:YD8119.14 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDR009W |
SGDi | S000002416, GAL3 |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184658 | 1 – 520 | Protein GAL3Add BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P13045 |
PaxDbi | P13045 |
PRIDEi | P13045 |
PTM databases
iPTMneti | P13045 |
Expressioni
Inductioni
The GAL3 gene is a member of the family of galactose inducible, glucose-repressible genes (which include GAL1, GAL2, GAL7, GAL10, GAL80, and MELI).
Interactioni
Binary interactionsi
P13045
With | #Exp. | IntAct |
---|---|---|
GAL80 [P04387] | 5 | EBI-7282,EBI-2061197 |
Protein-protein interaction databases
BioGRIDi | 32061, 64 interactors |
ComplexPortali | CPX-1042, GAL3-GAL80 transcription regulation complex |
DIPi | DIP-5725N |
IntActi | P13045, 4 interactors |
MINTi | P13045 |
STRINGi | 4932.YDR009W |
Miscellaneous databases
RNActi | P13045, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P13045 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0631, Eukaryota |
GeneTreei | ENSGT00950000183187 |
HOGENOMi | CLU_017814_6_2_1 |
InParanoidi | P13045 |
OMAi | HQEIDLQ |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.70.890, 1 hit |
InterProi | View protein in InterPro IPR000705, Galactokinase IPR019741, Galactokinase_CS IPR019539, GalKase_N IPR013750, GHMP_kinase_C_dom IPR036554, GHMP_kinase_C_sf IPR006204, GHMP_kinase_N_dom IPR006206, Mevalonate/galactokinase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
Pfami | View protein in Pfam PF10509, GalKase_gal_bdg, 1 hit PF08544, GHMP_kinases_C, 1 hit PF00288, GHMP_kinases_N, 1 hit |
PIRSFi | PIRSF000530, Galactokinase, 1 hit |
PRINTSi | PR00473, GALCTOKINASE |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55060, SSF55060, 1 hit |
TIGRFAMsi | TIGR00131, gal_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00106, GALACTOKINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P13045-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTNVPIFSS PVRDLPRSFE QKHLAVVDAF FQTYHVKPDF IARSPGRVNL
60 70 80 90 100
IGEHIDYCDF SVLPLAIDVD MLCAVKILDE KNPSITLTNA DPKFAQRKFD
110 120 130 140 150
LPLDGSYMAI DPSVSEWSNY FKCGLHVAHS YLKKIAPERF NNTPLVGAQI
160 170 180 190 200
FCQSDIPTGG GLSSAFTCAA ALATIRANMG KNFDISKKDL TRITAVAEHY
210 220 230 240 250
VGVNNGGMDQ ATSVYGEEDH ALYVEFRPKL KATPFKFPQL KNHEISFVIA
260 270 280 290 300
NTLVKSNKFE TAPTNYNLRV IEVTVAANAL ATRYSVALPS HKDNSNSERG
310 320 330 340 350
NLRDFMDAYY ARYENQAQPW NGDIGTGIER LLKMLQLVEE SFSRKKSGFT
360 370 380 390 400
VHEASTALNC SREEFTRDYL TTFPVRFQVL KLYQRAKHVY SESLRVLKAL
410 420 430 440 450
KMMTSATFHT DEDFFTDFGR LMNESQASCD KLYECSCIET NQICSIALAN
460 470 480 490 500
GSFGSRLTGA GWGGCTIHLV PSGANGNVEQ VRKALIEKFY NVRYPDLTDE
510 520
ELKDAIIVSK PALGTCLYEQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 – 45 | KPDFIARSP → NLILSLGLL in AAA34625 (PubMed:3062381).Curated | 9 | |
Sequence conflicti | 170 – 171 | AA → GR in AAA34625 (PubMed:3062381).Curated | 2 | |
Sequence conflicti | 192 – 211 | RITAV…GMDQA → ASQRLRSTMLESIMVVWIKQ in AAA34625 (PubMed:3062381).CuratedAdd BLAST | 20 | |
Sequence conflicti | 227 – 241 | RPKLK…FPQLK → MAKTKWPHFQVSSIE in AAA34625 (PubMed:3062381).CuratedAdd BLAST | 15 | |
Sequence conflicti | 251 – 294 | NTLVK…SHKDN → ILCTRSNNRTLLHISLSCSL LTLLYISTFAREPCGPDTLG LTISQGQ in AAA34625 (PubMed:3062381).CuratedAdd BLAST | 44 | |
Sequence conflicti | 303 – 338 | RDFMD…MLQLV → EIYGCLLRPDTKTKPNHGME ISELVLNVYSRCYNWY in AAA34625 (PubMed:3062381).CuratedAdd BLAST | 36 | |
Sequence conflicti | 407 – 425 | TFHTD…LMNES → IFTRTRFLYRFWPTNE in AAA34625 (PubMed:3062381).CuratedAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21615 Genomic DNA Translation: AAA34625.1 X95966 Genomic DNA Translation: CAA65201.1 Z48008 Genomic DNA Translation: CAA88069.1 Z74305 Genomic DNA Translation: CAA98829.1 AY723766 Genomic DNA Translation: AAU09683.1 BK006938 Genomic DNA Translation: DAA11856.1 |
PIRi | S50990 |
RefSeqi | NP_010292.1, NM_001180317.1 |
Genome annotation databases
EnsemblFungii | YDR009W_mRNA; YDR009W; YDR009W |
GeneIDi | 851572 |
KEGGi | sce:YDR009W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21615 Genomic DNA Translation: AAA34625.1 X95966 Genomic DNA Translation: CAA65201.1 Z48008 Genomic DNA Translation: CAA88069.1 Z74305 Genomic DNA Translation: CAA98829.1 AY723766 Genomic DNA Translation: AAU09683.1 BK006938 Genomic DNA Translation: DAA11856.1 |
PIRi | S50990 |
RefSeqi | NP_010292.1, NM_001180317.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3V2U | X-ray | 2.10 | C/D | 2-520 | [»] | |
3V5R | X-ray | 2.10 | A/B | 17-520 | [»] | |
SMRi | P13045 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32061, 64 interactors |
ComplexPortali | CPX-1042, GAL3-GAL80 transcription regulation complex |
DIPi | DIP-5725N |
IntActi | P13045, 4 interactors |
MINTi | P13045 |
STRINGi | 4932.YDR009W |
PTM databases
iPTMneti | P13045 |
Proteomic databases
MaxQBi | P13045 |
PaxDbi | P13045 |
PRIDEi | P13045 |
Genome annotation databases
EnsemblFungii | YDR009W_mRNA; YDR009W; YDR009W |
GeneIDi | 851572 |
KEGGi | sce:YDR009W |
Organism-specific databases
EuPathDBi | FungiDB:YDR009W |
SGDi | S000002416, GAL3 |
Phylogenomic databases
eggNOGi | KOG0631, Eukaryota |
GeneTreei | ENSGT00950000183187 |
HOGENOMi | CLU_017814_6_2_1 |
InParanoidi | P13045 |
OMAi | HQEIDLQ |
Miscellaneous databases
PROi | PR:P13045 |
RNActi | P13045, protein |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.70.890, 1 hit |
InterProi | View protein in InterPro IPR000705, Galactokinase IPR019741, Galactokinase_CS IPR019539, GalKase_N IPR013750, GHMP_kinase_C_dom IPR036554, GHMP_kinase_C_sf IPR006204, GHMP_kinase_N_dom IPR006206, Mevalonate/galactokinase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
Pfami | View protein in Pfam PF10509, GalKase_gal_bdg, 1 hit PF08544, GHMP_kinases_C, 1 hit PF00288, GHMP_kinases_N, 1 hit |
PIRSFi | PIRSF000530, Galactokinase, 1 hit |
PRINTSi | PR00473, GALCTOKINASE |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55060, SSF55060, 1 hit |
TIGRFAMsi | TIGR00131, gal_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00106, GALACTOKINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GAL3_YEAST | |
Accessioni | P13045Primary (citable) accession number: P13045 Secondary accession number(s): D6VRZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names