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Protein

Fe(3+) dicitrate transport protein FecA

Gene

fecA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FecA is the outer membrane receptor protein in the Fe3+ dicitrate transport system.

GO - Molecular functioni

  • ferric iron transmembrane transporter activity Source: EcoCyc
  • iron ion binding Source: InterPro
  • signaling receptor activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processIon transport, Iron transport, Transport
LigandIron

Enzyme and pathway databases

BioCyciEcoCyc:EG10286-MONOMER
MetaCyc:EG10286-MONOMER

Protein family/group databases

TCDBi1.B.14.1.20 the outer membrane receptor (omr) family

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+) dicitrate transport protein FecA
Alternative name(s):
Iron(III) dicitrate transport protein FecA
Gene namesi
Name:fecA
Ordered Locus Names:b4291, JW4251
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10286 fecA

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB04147 Lauryl Dimethylamine-N-Oxide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000003474634 – 774Fe(3+) dicitrate transport protein FecAAdd BLAST741

Proteomic databases

PaxDbiP13036
PRIDEiP13036

Expressioni

Inductioni

For induction, the TonB and the ExbB proteins have to be active. Regulation by the iron level mediated by the Fur protein and induction by citrate plus iron suggest that the Fe3+ dicitrate complex must enter the periplasm where it binds to a transmembrane protein, which regulates transcription of the fec genes directly or via a further inductor. Induced 2-fold by hydroxyurea (PubMed:20005847).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261554, 298 interactors
ComplexPortaliCPX-3576 Ferric-citrate outer membrane transporter complex
DIPiDIP-9583N
IntActiP13036, 3 interactors
STRINGi316385.ECDH10B_4489

Structurei

Secondary structure

1774
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13036
SMRiP13036
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13036

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi56 – 63TonB box8
Motifi757 – 774TonB C-terminal boxAdd BLAST18

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105GAV Bacteria
COG4772 LUCA
HOGENOMiHOG000219169
InParanoidiP13036
KOiK16091
PhylomeDBiP13036

Family and domain databases

Gene3Di2.170.130.10, 1 hit
2.40.170.20, 1 hit
InterProiView protein in InterPro
IPR012910 Plug_dom
IPR037066 Plug_dom_sf
IPR011662 Secretin/TonB_short_N
IPR000531 TonB-dep_rcpt_b-brl
IPR010916 TonB_box_CS
IPR036942 TonB_rcpt_b-brl_sf
IPR010917 TonB_rcpt_CS
IPR010105 TonB_sidphr_rcpt
PfamiView protein in Pfam
PF07715 Plug, 1 hit
PF07660 STN, 1 hit
PF00593 TonB_dep_Rec, 1 hit
SMARTiView protein in SMART
SM00965 STN, 1 hit
TIGRFAMsiTIGR01783 TonB-siderophor, 1 hit
PROSITEiView protein in PROSITE
PS00430 TONB_DEPENDENT_REC_1, 1 hit
PS01156 TONB_DEPENDENT_REC_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13036-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTPLRVFRKT TPLVNTIRLS LLPLAGLSFS AFAAQVNIAP GSLDKALNQY
60 70 80 90 100
AAHSGFTLSV DASLTRGKQS NGLHGDYDVE SGLQQLLDGS GLQVKPLGNN
110 120 130 140 150
SWTLEPAPAP KEDALTVVGD WLGDARENDV FEHAGARDVI RREDFAKTGA
160 170 180 190 200
TTMREVLNRI PGVSAPENNG TGSHDLAMNF GIRGLNPRLA SRSTVLMDGI
210 220 230 240 250
PVPFAPYGQP QLSLAPVSLG NMDAIDVVRG GGAVRYGPQS VGGVVNFVTR
260 270 280 290 300
AIPQDFGIEA GVEGQLSPTS SQNNPKETHN LMVGGTADNG FGTALLYSGT
310 320 330 340 350
RGSDWREHSA TRIDDLMLKS KYAPDEVHTF NSLLQYYDGE ADMPGGLSRA
360 370 380 390 400
DYDADRWQST RPYDRFWGRR KLASLGYQFQ PDSQHKFNIQ GFYTQTLRSG
410 420 430 440 450
YLEQGKRITL SPRNYWVRGI EPRYSQIFMI GPSAHEVGVG YRYLNESTHE
460 470 480 490 500
MRYYTATSSG QLPSGSSPYD RDTRSGTEAH AWYLDDKIDI GNWTITPGMR
510 520 530 540 550
FEHIESYQNN AITGTHEEVS YNAPLPALNV LYHLTDSWNL YANTEGSFGT
560 570 580 590 600
VQYSQIGKAV QSGNVEPEKA RTWELGTRYD DGALTAEMGL FLINFNNQYD
610 620 630 640 650
SNQTNDTVTA RGKTRHTGLE TQARYDLGTL TPTLDNVSIY ASYAYVNAEI
660 670 680 690 700
REKGDTYGNL VPFSPKHKGT LGVDYKPGNW TFNLNSDFQS SQFADNANTV
710 720 730 740 750
KESADGSTGR IPGFMLWGAR VAYDFGPQMA DLNLAFGVKN IFDQDYFIRS
760 770
YDDNNKGIYA GQPRTLYMQG SLKF
Length:774
Mass (Da):85,322
Last modified:February 1, 1995 - v2
Checksum:i16B5B510276C3B09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16T → A in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti190A → T in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti300T → M in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti300T → M (PubMed:2254251).Curated1
Sequence conflicti357W → R in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti444L → V in AAA23760 (PubMed:2836368).Curated1
Sequence conflicti749R → A in AAA23760 (PubMed:2836368).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20981 Genomic DNA Translation: AAA23760.1
U14003 Genomic DNA Translation: AAA97187.1
U00096 Genomic DNA Translation: AAC77247.1
AP009048 Genomic DNA Translation: BAE78282.1
M63115 Genomic DNA Translation: AAA23768.1
M26397 Genomic DNA Translation: AAA23761.1
PIRiE65242 QRECFA
RefSeqiNP_418711.1, NC_000913.3
WP_000188283.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77247; AAC77247; b4291
BAE78282; BAE78282; BAE78282
GeneIDi946427
KEGGiecj:JW4251
eco:b4291
PATRICifig|1411691.4.peg.2408

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20981 Genomic DNA Translation: AAA23760.1
U14003 Genomic DNA Translation: AAA97187.1
U00096 Genomic DNA Translation: AAC77247.1
AP009048 Genomic DNA Translation: BAE78282.1
M63115 Genomic DNA Translation: AAA23768.1
M26397 Genomic DNA Translation: AAA23761.1
PIRiE65242 QRECFA
RefSeqiNP_418711.1, NC_000913.3
WP_000188283.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMOX-ray2.00A1-774[»]
1KMPX-ray2.50A1-774[»]
1PNZX-ray2.50A34-774[»]
1PO0X-ray2.15A34-774[»]
1PO3X-ray3.40A/B34-774[»]
1ZZVNMR-A34-113[»]
2D1UNMR-A34-129[»]
ProteinModelPortaliP13036
SMRiP13036
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261554, 298 interactors
ComplexPortaliCPX-3576 Ferric-citrate outer membrane transporter complex
DIPiDIP-9583N
IntActiP13036, 3 interactors
STRINGi316385.ECDH10B_4489

Chemistry databases

DrugBankiDB04147 Lauryl Dimethylamine-N-Oxide

Protein family/group databases

TCDBi1.B.14.1.20 the outer membrane receptor (omr) family

Proteomic databases

PaxDbiP13036
PRIDEiP13036

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77247; AAC77247; b4291
BAE78282; BAE78282; BAE78282
GeneIDi946427
KEGGiecj:JW4251
eco:b4291
PATRICifig|1411691.4.peg.2408

Organism-specific databases

EchoBASEiEB0282
EcoGeneiEG10286 fecA

Phylogenomic databases

eggNOGiENOG4105GAV Bacteria
COG4772 LUCA
HOGENOMiHOG000219169
InParanoidiP13036
KOiK16091
PhylomeDBiP13036

Enzyme and pathway databases

BioCyciEcoCyc:EG10286-MONOMER
MetaCyc:EG10286-MONOMER

Miscellaneous databases

EvolutionaryTraceiP13036
PROiPR:P13036

Family and domain databases

Gene3Di2.170.130.10, 1 hit
2.40.170.20, 1 hit
InterProiView protein in InterPro
IPR012910 Plug_dom
IPR037066 Plug_dom_sf
IPR011662 Secretin/TonB_short_N
IPR000531 TonB-dep_rcpt_b-brl
IPR010916 TonB_box_CS
IPR036942 TonB_rcpt_b-brl_sf
IPR010917 TonB_rcpt_CS
IPR010105 TonB_sidphr_rcpt
PfamiView protein in Pfam
PF07715 Plug, 1 hit
PF07660 STN, 1 hit
PF00593 TonB_dep_Rec, 1 hit
SMARTiView protein in SMART
SM00965 STN, 1 hit
TIGRFAMsiTIGR01783 TonB-siderophor, 1 hit
PROSITEiView protein in PROSITE
PS00430 TONB_DEPENDENT_REC_1, 1 hit
PS01156 TONB_DEPENDENT_REC_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFECA_ECOLI
AccessioniPrimary (citable) accession number: P13036
Secondary accession number(s): Q2M624
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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