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Protein

Aerobic glycerol-3-phosphate dehydrogenase

Gene

glpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.

Miscellaneous

There are two sn-glycerol-3-phosphate dehydrogenase isozymes in E.coli: one is aerobic, the other anaerobic.

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (aerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Aerobic glycerol-3-phosphate dehydrogenase (glpD)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (aerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 33FADSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

  • glycerol-3-phosphate catabolic process Source: EcoCyc
  • glycerol catabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processGlycerol metabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:AERGLYC3PDEHYDROG-MONOMER
MetaCyc:AERGLYC3PDEHYDROG-MONOMER
BRENDAi1.1.1.94 2026
UniPathwayi
UPA00618;UER00674

Names & Taxonomyi

Protein namesi
Recommended name:
Aerobic glycerol-3-phosphate dehydrogenase (EC:1.1.5.3)
Gene namesi
Name:glpD
Synonyms:glyD
Ordered Locus Names:b3426, JW3389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10394 glpD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001260981 – 501Aerobic glycerol-3-phosphate dehydrogenaseAdd BLAST501

Proteomic databases

EPDiP13035
PaxDbiP13035
PRIDEiP13035

2D gel databases

SWISS-2DPAGEiP13035

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
damP0AEE83EBI-548509,EBI-548491

Protein-protein interaction databases

BioGridi4261265, 432 interactors
DIPiDIP-9793N
IntActiP13035, 91 interactors
STRINGi316385.ECDH10B_3600

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13035
SMRiP13035
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13035

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C6V Bacteria
COG0578 LUCA
HOGENOMiHOG000004811
InParanoidiP13035
KOiK00111
PhylomeDBiP13035

Family and domain databases

Gene3Di1.10.8.870, 1 hit
3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR031656 DAO_C
IPR038299 DAO_C_sf
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR000447 G3P_DH_FAD-dep
PANTHERiPTHR11985 PTHR11985, 1 hit
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PF16901 DAO_C, 1 hit
PRINTSiPR01001 FADG3PDH
SUPFAMiSSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS00977 FAD_G3PDH_1, 1 hit
PS00978 FAD_G3PDH_2, 1 hit

Sequencei

Sequence statusi: Complete.

P13035-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METKDLIVIG GGINGAGIAA DAAGRGLSVL MLEAQDLACA TSSASSKLIH
60 70 80 90 100
GGLRYLEHYE FRLVSEALAE REVLLKMAPH IAFPMRFRLP HRPHLRPAWM
110 120 130 140 150
IRIGLFMYDH LGKRTSLPGS TGLRFGANSV LKPEIKRGFE YSDCWVDDAR
160 170 180 190 200
LVLANAQMVV RKGGEVLTRT RATSARRENG LWIVEAEDID TGKKYSWQAR
210 220 230 240 250
GLVNATGPWV KQFFDDGMHL PSPYGIRLIK GSHIVVPRVH TQKQAYILQN
260 270 280 290 300
EDKRIVFVIP WMDEFSIIGT TDVEYKGDPK AVKIEESEIN YLLNVYNTHF
310 320 330 340 350
KKQLSRDDIV WTYSGVRPLC DDESDSPQAI TRDYTLDIHD ENGKAPLLSV
360 370 380 390 400
FGGKLTTYRK LAEHALEKLT PYYQGIGPAW TKESVLPGGA IEGDRDDYAA
410 420 430 440 450
RLRRRYPFLT ESLARHYART YGSNSELLLG NAGTVSDLGE DFGHEFYEAE
460 470 480 490 500
LKYLVDHEWV RRADDALWRR TKQGMWLNAD QQSRVSQWLV EYTQQRLSLA

S
Length:501
Mass (Da):56,751
Last modified:May 1, 1992 - v3
Checksum:i1C4D341E9E4536AB
GO

Sequence cautioni

The sequence AAA23888 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23A → G in BAA00327 (Ref. 2) Curated1
Sequence conflicti328 – 353QAITR…SVFGG → HVLPVITPLIFMMKMAKHRC CRYSAA in BAA00327 (Ref. 2) CuratedAdd BLAST26
Sequence conflicti464 – 501DDALW…LSLAS → ARRPVASHKTRHVAKCGSTI SCESVAGGVYAAEVIAGVVN in BAA00327 (Ref. 2) CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55989 Genomic DNA Translation: AAA24636.1
M96795 Genomic DNA Translation: AAC28164.1
D00425 Genomic DNA Translation: BAA00327.1
U18997 Genomic DNA Translation: AAA58224.1
U00096 Genomic DNA Translation: AAC76451.1
AP009048 Genomic DNA Translation: BAE77866.1
M21277 Genomic DNA Translation: AAA23885.1
M54940 Genomic DNA Translation: AAA23888.1 Different initiation.
PIRiA39186 DEECGD
RefSeqiNP_417884.1, NC_000913.3
WP_000448136.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76451; AAC76451; b3426
BAE77866; BAE77866; BAE77866
GeneIDi947934
KEGGiecj:JW3389
eco:b3426
PATRICifig|511145.12.peg.3521

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55989 Genomic DNA Translation: AAA24636.1
M96795 Genomic DNA Translation: AAC28164.1
D00425 Genomic DNA Translation: BAA00327.1
U18997 Genomic DNA Translation: AAA58224.1
U00096 Genomic DNA Translation: AAC76451.1
AP009048 Genomic DNA Translation: BAE77866.1
M21277 Genomic DNA Translation: AAA23885.1
M54940 Genomic DNA Translation: AAA23888.1 Different initiation.
PIRiA39186 DEECGD
RefSeqiNP_417884.1, NC_000913.3
WP_000448136.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QCUX-ray1.75A/B1-501[»]
2R45X-ray2.30A/B1-501[»]
2R46X-ray2.10A/B1-501[»]
2R4EX-ray2.10A/B1-501[»]
2R4JX-ray1.96A/B1-501[»]
ProteinModelPortaliP13035
SMRiP13035
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261265, 432 interactors
DIPiDIP-9793N
IntActiP13035, 91 interactors
STRINGi316385.ECDH10B_3600

2D gel databases

SWISS-2DPAGEiP13035

Proteomic databases

EPDiP13035
PaxDbiP13035
PRIDEiP13035

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76451; AAC76451; b3426
BAE77866; BAE77866; BAE77866
GeneIDi947934
KEGGiecj:JW3389
eco:b3426
PATRICifig|511145.12.peg.3521

Organism-specific databases

EchoBASEiEB0389
EcoGeneiEG10394 glpD

Phylogenomic databases

eggNOGiENOG4105C6V Bacteria
COG0578 LUCA
HOGENOMiHOG000004811
InParanoidiP13035
KOiK00111
PhylomeDBiP13035

Enzyme and pathway databases

UniPathwayi
UPA00618;UER00674

BioCyciEcoCyc:AERGLYC3PDEHYDROG-MONOMER
MetaCyc:AERGLYC3PDEHYDROG-MONOMER
BRENDAi1.1.1.94 2026

Miscellaneous databases

EvolutionaryTraceiP13035
PROiPR:P13035

Family and domain databases

Gene3Di1.10.8.870, 1 hit
3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR031656 DAO_C
IPR038299 DAO_C_sf
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR000447 G3P_DH_FAD-dep
PANTHERiPTHR11985 PTHR11985, 1 hit
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PF16901 DAO_C, 1 hit
PRINTSiPR01001 FADG3PDH
SUPFAMiSSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS00977 FAD_G3PDH_1, 1 hit
PS00978 FAD_G3PDH_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLPD_ECOLI
AccessioniPrimary (citable) accession number: P13035
Secondary accession number(s): P78115, Q2M790, Q47234
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 1, 1992
Last modified: November 7, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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