UniProtKB - P13033 (GLPB_ECOLI)
Protein
Anaerobic glycerol-3-phosphate dehydrogenase subunit B
Gene
glpB
Organism
Escherichia coli (strain K12)
Status
Functioni
Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.
Catalytic activityi
- EC:1.1.5.3
Cofactori
: glycerol degradation via glycerol kinase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).Proteins known to be involved in this subpathway in this organism are:
- Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB), Anaerobic glycerol-3-phosphate dehydrogenase subunit C (glpC), Anaerobic glycerol-3-phosphate dehydrogenase subunit A (glpA), Glycerol-3-phosphate dehydrogenase (glpA), Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.
GO - Molecular functioni
- FMN binding Source: EcoCyc
- glycerol-3-phosphate dehydrogenase (quinone) activity Source: EcoCyc
- sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- glycerol-3-phosphate catabolic process Source: EcoCyc
- glycerol catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
BioCyci | EcoCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER MetaCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER |
UniPathwayi | UPA00618;UER00673 |
Names & Taxonomyi
Protein namesi | Recommended name: Anaerobic glycerol-3-phosphate dehydrogenase subunit B (EC:1.1.5.3)Short name: Anaerobic G-3-P dehydrogenase subunit B Short name: Anaerobic G3Pdhase B |
Gene namesi | Name:glpB Ordered Locus Names:b2242, JW2236 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- glycerol-3-phosphate dehydrogenase complex Source: GO_Central
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204559 | 1 – 419 | Anaerobic glycerol-3-phosphate dehydrogenase subunit BAdd BLAST | 419 |
Proteomic databases
PaxDbi | P13033 |
PRIDEi | P13033 |
Interactioni
Subunit structurei
Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.
Protein-protein interaction databases
BioGRIDi | 4260490, 447 interactors 851074, 1 interactor |
ComplexPortali | CPX-4841, Anaerobic glycerol-3-phosphate dehydrogenase complex |
DIPi | DIP-9791N |
IntActi | P13033, 5 interactors |
STRINGi | 511145.b2242 |
Family & Domainsi
Sequence similaritiesi
Belongs to the anaerobic G-3-P dehydrogenase subunit B family.Curated
Phylogenomic databases
eggNOGi | COG3075, Bacteria |
HOGENOMi | CLU_047793_0_0_6 |
InParanoidi | P13033 |
PhylomeDBi | P13033 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00753, Glycerol3P_GlpB, 1 hit |
InterProi | View protein in InterPro IPR003953, FAD-binding_2 IPR036188, FAD/NAD-bd_sf IPR009158, G3P_DH_GlpB_su |
Pfami | View protein in Pfam PF00890, FAD_binding_2, 1 hit |
PIRSFi | PIRSF000141, Anaerobic_G3P_dh, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03378, glycerol3P_GlpB, 1 hit |
i Sequence
Sequence statusi: Complete.
P13033-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFDTVIMGG GLAGLLCGLQ LQKHGLRCAI VTRGQSALHF SSGSLDLLSH
60 70 80 90 100
LPDGQPVTDI HSGLESLRQQ APAHPYSLLE PQRVLDLACQ AQALIAESGA
110 120 130 140 150
QLQGSVELAH QRVTPLGTLR STWLSSPEVP VWPLPAKKIC VVGISGLMDF
160 170 180 190 200
QAHLAAASLR ELGLAVETAE IELPELDVLR NNATEFRAVN IARFLDNEEN
210 220 230 240 250
WPLLLDALIP VANTCEMILM PACFGLADDK LWRWLNEKLP CSLMLLPTLP
260 270 280 290 300
PSVLGIRLQN QLQRQFVRQG GVWMPGDEVK KVTCKNGVVN EIWTRNHADI
310 320 330 340 350
PLRPRFAVLA SGSFFSGGLV AERNGIREPI LGLDVLQTAT RGEWYKGDFF
360 370 380 390 400
APQPWQQFGV TTDETLRPSQ AGQTIENLFA IGSVLGGFDP IAQGCGGGVC
410
AVSALHAAQQ IAQRAGGQQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20938 Genomic DNA Translation: AAA83865.1 U00096 Genomic DNA Translation: AAC75302.1 AP009048 Genomic DNA Translation: BAA16061.1 |
PIRi | B32006, DEECNB |
RefSeqi | NP_416745.1, NC_000913.3 WP_001209927.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75302; AAC75302; b2242 BAA16061; BAA16061; BAA16061 |
GeneIDi | 57727980 946733 |
KEGGi | ecj:JW2236 eco:b2242 |
PATRICi | fig|511145.12.peg.2331 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20938 Genomic DNA Translation: AAA83865.1 U00096 Genomic DNA Translation: AAC75302.1 AP009048 Genomic DNA Translation: BAA16061.1 |
PIRi | B32006, DEECNB |
RefSeqi | NP_416745.1, NC_000913.3 WP_001209927.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260490, 447 interactors 851074, 1 interactor |
ComplexPortali | CPX-4841, Anaerobic glycerol-3-phosphate dehydrogenase complex |
DIPi | DIP-9791N |
IntActi | P13033, 5 interactors |
STRINGi | 511145.b2242 |
Proteomic databases
PaxDbi | P13033 |
PRIDEi | P13033 |
Genome annotation databases
EnsemblBacteriai | AAC75302; AAC75302; b2242 BAA16061; BAA16061; BAA16061 |
GeneIDi | 57727980 946733 |
KEGGi | ecj:JW2236 eco:b2242 |
PATRICi | fig|511145.12.peg.2331 |
Organism-specific databases
EchoBASEi | EB0387 |
Phylogenomic databases
eggNOGi | COG3075, Bacteria |
HOGENOMi | CLU_047793_0_0_6 |
InParanoidi | P13033 |
PhylomeDBi | P13033 |
Enzyme and pathway databases
UniPathwayi | UPA00618;UER00673 |
BioCyci | EcoCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER MetaCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER |
Miscellaneous databases
PROi | PR:P13033 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00753, Glycerol3P_GlpB, 1 hit |
InterProi | View protein in InterPro IPR003953, FAD-binding_2 IPR036188, FAD/NAD-bd_sf IPR009158, G3P_DH_GlpB_su |
Pfami | View protein in Pfam PF00890, FAD_binding_2, 1 hit |
PIRSFi | PIRSF000141, Anaerobic_G3P_dh, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03378, glycerol3P_GlpB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLPB_ECOLI | |
Accessioni | P13033Primary (citable) accession number: P13033 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families